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BAT26 Microsatellite Marker Polymorphism in Colorectal Cancer in Senegalese Patients

Received: 11 June 2022     Accepted: 30 June 2022     Published: 12 July 2022
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Abstract

Colorectal cancer is a serious problem in cancerology due to its frequency and severity. It is the third most common form of cancer, with one million new cases worldwide each year, and its mortality is around 30%. Despite significant advances in "omics" techniques that allow us to understand the molecular mechanisms involved in the evolution of cancer pathologies, the strongest risk factors currently involved in the ethology of this cancer are those of a genetic nature, with specific mutations. The aim of this study is therefore to accurately establish the incidence of the MSI phenotype in Senegalese patients with colorectal cancer using the BAT26 single nucleotide marker. To identify these phenotypes, the BAT26 loci sequences of 18 cancer patients were compared with a reference sequence and their haplotypes were determined through DnaSP version 5.10. The MEGA software version 7.014 and the Arlequin program version 3.1 were used to highlight the genetic differentiation as well as the demo-genetic evolution of our study populations. The results of this comparison revealed a high microsatellite instability. This nucleotide difference is materialized by adenine to guanine substitutions present in the sequences of 17 cancer patients. This variability between cancer tissues is confirmed by the Nei genetic distance which shows that cancer tissues are genetically heterogeneous. The Fst indicates that there is genetic structuring between the cancer tissue sequences and our control (the reference sequence). These results suggest that this polymorphism of the BAT26 single-nucleotide marker may be involved in the occurrence of this cancer.

Published in International Journal of Genetics and Genomics (Volume 10, Issue 3)
DOI 10.11648/j.ijgg.20221003.11
Page(s) 59-63
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

BAT26, Colorectal Cancer, Instability, Microsatellite, Variability

References
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Cite This Article
  • APA Style

    Seye Yacine, Ndong Anna, Keneme Bineta, Sembene Pape Mbacke. (2022). BAT26 Microsatellite Marker Polymorphism in Colorectal Cancer in Senegalese Patients. International Journal of Genetics and Genomics, 10(3), 59-63. https://doi.org/10.11648/j.ijgg.20221003.11

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    ACS Style

    Seye Yacine; Ndong Anna; Keneme Bineta; Sembene Pape Mbacke. BAT26 Microsatellite Marker Polymorphism in Colorectal Cancer in Senegalese Patients. Int. J. Genet. Genomics 2022, 10(3), 59-63. doi: 10.11648/j.ijgg.20221003.11

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    AMA Style

    Seye Yacine, Ndong Anna, Keneme Bineta, Sembene Pape Mbacke. BAT26 Microsatellite Marker Polymorphism in Colorectal Cancer in Senegalese Patients. Int J Genet Genomics. 2022;10(3):59-63. doi: 10.11648/j.ijgg.20221003.11

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  • @article{10.11648/j.ijgg.20221003.11,
      author = {Seye Yacine and Ndong Anna and Keneme Bineta and Sembene Pape Mbacke},
      title = {BAT26 Microsatellite Marker Polymorphism in Colorectal Cancer in Senegalese Patients},
      journal = {International Journal of Genetics and Genomics},
      volume = {10},
      number = {3},
      pages = {59-63},
      doi = {10.11648/j.ijgg.20221003.11},
      url = {https://doi.org/10.11648/j.ijgg.20221003.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20221003.11},
      abstract = {Colorectal cancer is a serious problem in cancerology due to its frequency and severity. It is the third most common form of cancer, with one million new cases worldwide each year, and its mortality is around 30%. Despite significant advances in "omics" techniques that allow us to understand the molecular mechanisms involved in the evolution of cancer pathologies, the strongest risk factors currently involved in the ethology of this cancer are those of a genetic nature, with specific mutations. The aim of this study is therefore to accurately establish the incidence of the MSI phenotype in Senegalese patients with colorectal cancer using the BAT26 single nucleotide marker. To identify these phenotypes, the BAT26 loci sequences of 18 cancer patients were compared with a reference sequence and their haplotypes were determined through DnaSP version 5.10. The MEGA software version 7.014 and the Arlequin program version 3.1 were used to highlight the genetic differentiation as well as the demo-genetic evolution of our study populations. The results of this comparison revealed a high microsatellite instability. This nucleotide difference is materialized by adenine to guanine substitutions present in the sequences of 17 cancer patients. This variability between cancer tissues is confirmed by the Nei genetic distance which shows that cancer tissues are genetically heterogeneous. The Fst indicates that there is genetic structuring between the cancer tissue sequences and our control (the reference sequence). These results suggest that this polymorphism of the BAT26 single-nucleotide marker may be involved in the occurrence of this cancer.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - BAT26 Microsatellite Marker Polymorphism in Colorectal Cancer in Senegalese Patients
    AU  - Seye Yacine
    AU  - Ndong Anna
    AU  - Keneme Bineta
    AU  - Sembene Pape Mbacke
    Y1  - 2022/07/12
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijgg.20221003.11
    DO  - 10.11648/j.ijgg.20221003.11
    T2  - International Journal of Genetics and Genomics
    JF  - International Journal of Genetics and Genomics
    JO  - International Journal of Genetics and Genomics
    SP  - 59
    EP  - 63
    PB  - Science Publishing Group
    SN  - 2376-7359
    UR  - https://doi.org/10.11648/j.ijgg.20221003.11
    AB  - Colorectal cancer is a serious problem in cancerology due to its frequency and severity. It is the third most common form of cancer, with one million new cases worldwide each year, and its mortality is around 30%. Despite significant advances in "omics" techniques that allow us to understand the molecular mechanisms involved in the evolution of cancer pathologies, the strongest risk factors currently involved in the ethology of this cancer are those of a genetic nature, with specific mutations. The aim of this study is therefore to accurately establish the incidence of the MSI phenotype in Senegalese patients with colorectal cancer using the BAT26 single nucleotide marker. To identify these phenotypes, the BAT26 loci sequences of 18 cancer patients were compared with a reference sequence and their haplotypes were determined through DnaSP version 5.10. The MEGA software version 7.014 and the Arlequin program version 3.1 were used to highlight the genetic differentiation as well as the demo-genetic evolution of our study populations. The results of this comparison revealed a high microsatellite instability. This nucleotide difference is materialized by adenine to guanine substitutions present in the sequences of 17 cancer patients. This variability between cancer tissues is confirmed by the Nei genetic distance which shows that cancer tissues are genetically heterogeneous. The Fst indicates that there is genetic structuring between the cancer tissue sequences and our control (the reference sequence). These results suggest that this polymorphism of the BAT26 single-nucleotide marker may be involved in the occurrence of this cancer.
    VL  - 10
    IS  - 3
    ER  - 

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Author Information
  • Faculty of Medicine, Pharmacy and Odontology, Cheikh Anta Diop University, Dakar, Senegal

  • Department of Animal Biology, Cheikh Anta Diop University, Dakar, Senegal

  • Department of Animal Biology, Cheikh Anta Diop University, Dakar, Senegal

  • Department of Animal Biology, Cheikh Anta Diop University, Dakar, Senegal

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