Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan

Nasir Abbas; Muhammad Suleman; Aamer Bin Zahur; Abdul Ghafoor; Farooq Rashid; Amin Ullah Jan; Fazal Akbar; Shahid Ali; Abdul Aziz; Ziaul Islam; Abdullah Shah

doi:doi:10.11648/j.ijgg.20190703.17

Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan

Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, Amin Ullah Jan, Fazal Akbar, Shahid Ali, Abdul Aziz, Ziaul Islam, Abdullah Shah

Published in International Journal of Genetics and Genomics (Volume 7, Issue 3)

Received: 3 April 2019 Accepted: 17 May 2019 Published: 23 September 2019

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Abstract

Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.

Published in	International Journal of Genetics and Genomics (Volume 7, Issue 3)
DOI	10.11648/j.ijgg.20190703.17
Page(s)	75-79
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Leptin, Sahiwal, Achai, Nili-Ravi, Polymorphism, PCR-RFLP

References

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APA Style

Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, et al. (2019). Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. International Journal of Genetics and Genomics, 7(3), 75-79. https://doi.org/10.11648/j.ijgg.20190703.17

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ACS Style

Nasir Abbas; Muhammad Suleman; Aamer Bin Zahur; Abdul Ghafoor; Farooq Rashid, et al. Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. Int. J. Genet. Genomics 2019, 7(3), 75-79. doi: 10.11648/j.ijgg.20190703.17

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AMA Style

Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, et al. Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. Int J Genet Genomics. 2019;7(3):75-79. doi: 10.11648/j.ijgg.20190703.17

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@article{10.11648/j.ijgg.20190703.17,
  author = {Nasir Abbas and Muhammad Suleman and Aamer Bin Zahur and Abdul Ghafoor and Farooq Rashid and Amin Ullah Jan and Fazal Akbar and Shahid Ali and Abdul Aziz and Ziaul Islam and Abdullah Shah},
  title = {Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan},
  journal = {International Journal of Genetics and Genomics},
  volume = {7},
  number = {3},
  pages = {75-79},
  doi = {10.11648/j.ijgg.20190703.17},
  url = {https://doi.org/10.11648/j.ijgg.20190703.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190703.17},
  abstract = {Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.},
 year = {2019}
}

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TY  - JOUR
T1  - Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan
AU  - Nasir Abbas
AU  - Muhammad Suleman
AU  - Aamer Bin Zahur
AU  - Abdul Ghafoor
AU  - Farooq Rashid
AU  - Amin Ullah Jan
AU  - Fazal Akbar
AU  - Shahid Ali
AU  - Abdul Aziz
AU  - Ziaul Islam
AU  - Abdullah Shah
Y1  - 2019/09/23
PY  - 2019
N1  - https://doi.org/10.11648/j.ijgg.20190703.17
DO  - 10.11648/j.ijgg.20190703.17
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 75
EP  - 79
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20190703.17
AB  - Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.
VL  - 7
IS  - 3
ER  -

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Author Information

Nasir Abbas

National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan
Muhammad Suleman

Key State Laboratory of Cellular Stress Biology, Schoolof Life Sciences, Xiamen University, Xiamen, China
Aamer Bin Zahur

National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan
Abdul Ghafoor

Centre for Biotechnology and Microbiology, University of Swat, Swat, Pakistan
Farooq Rashid

National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan
Amin Ullah Jan

Department of Biotechnology Shaheed Benazir Bhutto University Sheringal Dir Upper, Khyber Pakhtunkhwa, Pakistan
Fazal Akbar

Key State Laboratory of Cellular Stress Biology, Schoolof Life Sciences, Xiamen University, Xiamen, China
Shahid Ali

Key State Laboratory of Cellular Stress Biology, Schoolof Life Sciences, Xiamen University, Xiamen, China
Abdul Aziz

National Institute for Genomics and Advanced Biotechnology (NIGAB), National Agricultural Research Centre, Islamabad, Pakistan
Ziaul Islam

Department of Biotechnology Shaheed Benazir Bhutto University Sheringal Dir Upper, Khyber Pakhtunkhwa, Pakistan
Abdullah Shah

Department of Biotechnology Shaheed Benazir Bhutto University Sheringal Dir Upper, Khyber Pakhtunkhwa, Pakistan

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APA Style

Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, et al. (2019). Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. International Journal of Genetics and Genomics, 7(3), 75-79. https://doi.org/10.11648/j.ijgg.20190703.17

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ACS Style

Nasir Abbas; Muhammad Suleman; Aamer Bin Zahur; Abdul Ghafoor; Farooq Rashid, et al. Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. Int. J. Genet. Genomics 2019, 7(3), 75-79. doi: 10.11648/j.ijgg.20190703.17

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AMA Style

Nasir Abbas, Muhammad Suleman, Aamer Bin Zahur, Abdul Ghafoor, Farooq Rashid, et al. Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan. Int J Genet Genomics. 2019;7(3):75-79. doi: 10.11648/j.ijgg.20190703.17

Copy | Download

@article{10.11648/j.ijgg.20190703.17,
  author = {Nasir Abbas and Muhammad Suleman and Aamer Bin Zahur and Abdul Ghafoor and Farooq Rashid and Amin Ullah Jan and Fazal Akbar and Shahid Ali and Abdul Aziz and Ziaul Islam and Abdullah Shah},
  title = {Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan},
  journal = {International Journal of Genetics and Genomics},
  volume = {7},
  number = {3},
  pages = {75-79},
  doi = {10.11648/j.ijgg.20190703.17},
  url = {https://doi.org/10.11648/j.ijgg.20190703.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20190703.17},
  abstract = {Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Molecular Analysis of Leptin Gene Polymorphism in Achai, Sahiwal Cattle and Nili-ravi Buffalo Breeds of Pakistan
AU  - Nasir Abbas
AU  - Muhammad Suleman
AU  - Aamer Bin Zahur
AU  - Abdul Ghafoor
AU  - Farooq Rashid
AU  - Amin Ullah Jan
AU  - Fazal Akbar
AU  - Shahid Ali
AU  - Abdul Aziz
AU  - Ziaul Islam
AU  - Abdullah Shah
Y1  - 2019/09/23
PY  - 2019
N1  - https://doi.org/10.11648/j.ijgg.20190703.17
DO  - 10.11648/j.ijgg.20190703.17
T2  - International Journal of Genetics and Genomics
JF  - International Journal of Genetics and Genomics
JO  - International Journal of Genetics and Genomics
SP  - 75
EP  - 79
PB  - Science Publishing Group
SN  - 2376-7359
UR  - https://doi.org/10.11648/j.ijgg.20190703.17
AB  - Pakistan is blessed with rich sheep genetic resources and being a source of milk, meat and wool sheep breeds are reared across the country. Despite its great economic importance very limited work has been done in Pakistan on sheep genetic exploration. Leptin hormone plays important role in milk yield, body weight, energy balance, feed intake, immune function and fertility performance in animals. The current study was carried out to investigate genetic polymorphism in exon III of Leptin gene (LEP) in indigenous animal breeds (Nilli-ravi buffalo, Sahiwal and Achai cattle) of Pakistan using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). PCR-RFLP analysis revealed that all the three studied animals breeds were polymorphic for i>LEP gene (having all the three genotypes AA, AB and BB) with frequency of 60%, 33% and 7% in Nili-ravi buffalo, 55%, 41% and 4% in Achai cattle and 52%, 45% and 3% in Sahiwal cattle breed. The genotype BB is highly desirable in cattle and buffalo breeds but its frequency is very low in our indigenous population. Studying genetic polymorphism of milk protein is important due to its application in dairy industry and these in formations could further be utilized for future breeding programs particularly for the enrichment of the rare genotype.
VL  - 7
IS  - 3
ER  -

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