Acacia Senegal var. senegal (Gum Arabic) as it is known world-wide, is one of the most important trees in Sudan with valuable contribution to the national income export commodity. Gum Arabic is an important ingridunt in several medicinal and food products. Little is known on the genetic markers and diversity of Acacia senegal. To our knowledge, this is the first study represents attempt to characterize genotypic variability of Acacia senegal var. senegal. The aim of this study was the molecular characterization of Acacia senegal var senegal using RAPD technique as molecular marker and to correlate the results obtained from genetic marker with the production and the geographical distribution of the trees. Acacia seeds were collected from different geographical areas of Sudan. Seeds of trees Acacia senegal var. senegal were cultivated in small pots. The seeds were germinated for 4 to 6 weeks then the new leaves were collected for DNA extraction using CTAB protocol. DNA was also extracted from the roots. Two octamer RAPD primers were evaluated for amplification of Acacia DNA. Phylogenetic tree was constructed based on RAPD-PCR which clustered the trees from each geographic area as separate group that were genetically related. The data showed a common ancestor of the three clusters suggesting.
| Published in | International Journal of Genetics and Genomics (Volume 4, Issue 3) |
| DOI | 10.11648/j.ijgg.20160403.12 |
| Page(s) | 20-23 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
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Copyright © The Author(s), 2016. Published by Science Publishing Group |
Acacia Senegal var. senegal, Molecular Characterization, RAPD
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APA Style
Saad Samah Mahgoub Hassan Mohammed, Mukhtar Moawia Mohammed, Warag Essam Eelldin, Elamin Hassan Basher, Aycan Murat, et al. (2016). Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD). International Journal of Genetics and Genomics, 4(3), 20-23. https://doi.org/10.11648/j.ijgg.20160403.12
ACS Style
Saad Samah Mahgoub Hassan Mohammed; Mukhtar Moawia Mohammed; Warag Essam Eelldin; Elamin Hassan Basher; Aycan Murat, et al. Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD). Int. J. Genet. Genomics 2016, 4(3), 20-23. doi: 10.11648/j.ijgg.20160403.12
AMA Style
Saad Samah Mahgoub Hassan Mohammed, Mukhtar Moawia Mohammed, Warag Essam Eelldin, Elamin Hassan Basher, Aycan Murat, et al. Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD). Int J Genet Genomics. 2016;4(3):20-23. doi: 10.11648/j.ijgg.20160403.12
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Download@article{10.11648/j.ijgg.20160403.12,
author = {Saad Samah Mahgoub Hassan Mohammed and Mukhtar Moawia Mohammed and Warag Essam Eelldin and Elamin Hassan Basher and Aycan Murat and Yıldız Mustafa},
title = {Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD)},
journal = {International Journal of Genetics and Genomics},
volume = {4},
number = {3},
pages = {20-23},
doi = {10.11648/j.ijgg.20160403.12},
url = {https://doi.org/10.11648/j.ijgg.20160403.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20160403.12},
abstract = {Acacia Senegal var. senegal (Gum Arabic) as it is known world-wide, is one of the most important trees in Sudan with valuable contribution to the national income export commodity. Gum Arabic is an important ingridunt in several medicinal and food products. Little is known on the genetic markers and diversity of Acacia senegal. To our knowledge, this is the first study represents attempt to characterize genotypic variability of Acacia senegal var. senegal. The aim of this study was the molecular characterization of Acacia senegal var senegal using RAPD technique as molecular marker and to correlate the results obtained from genetic marker with the production and the geographical distribution of the trees. Acacia seeds were collected from different geographical areas of Sudan. Seeds of trees Acacia senegal var. senegal were cultivated in small pots. The seeds were germinated for 4 to 6 weeks then the new leaves were collected for DNA extraction using CTAB protocol. DNA was also extracted from the roots. Two octamer RAPD primers were evaluated for amplification of Acacia DNA. Phylogenetic tree was constructed based on RAPD-PCR which clustered the trees from each geographic area as separate group that were genetically related. The data showed a common ancestor of the three clusters suggesting.},
year = {2016}
}
TY - JOUR T1 - Characterization of Phenotypic Variability of Sudanese Gum Arabic Tree Acacia Senegal var. senegal Using Multiprimer Random Amplificatıon of Polymorphic DNA (RAPD) AU - Saad Samah Mahgoub Hassan Mohammed AU - Mukhtar Moawia Mohammed AU - Warag Essam Eelldin AU - Elamin Hassan Basher AU - Aycan Murat AU - Yıldız Mustafa Y1 - 2016/06/20 PY - 2016 N1 - https://doi.org/10.11648/j.ijgg.20160403.12 DO - 10.11648/j.ijgg.20160403.12 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 20 EP - 23 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20160403.12 AB - Acacia Senegal var. senegal (Gum Arabic) as it is known world-wide, is one of the most important trees in Sudan with valuable contribution to the national income export commodity. Gum Arabic is an important ingridunt in several medicinal and food products. Little is known on the genetic markers and diversity of Acacia senegal. To our knowledge, this is the first study represents attempt to characterize genotypic variability of Acacia senegal var. senegal. The aim of this study was the molecular characterization of Acacia senegal var senegal using RAPD technique as molecular marker and to correlate the results obtained from genetic marker with the production and the geographical distribution of the trees. Acacia seeds were collected from different geographical areas of Sudan. Seeds of trees Acacia senegal var. senegal were cultivated in small pots. The seeds were germinated for 4 to 6 weeks then the new leaves were collected for DNA extraction using CTAB protocol. DNA was also extracted from the roots. Two octamer RAPD primers were evaluated for amplification of Acacia DNA. Phylogenetic tree was constructed based on RAPD-PCR which clustered the trees from each geographic area as separate group that were genetically related. The data showed a common ancestor of the three clusters suggesting. VL - 4 IS - 3 ER -
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