An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Transcribed Spacer (ITS 4-5) genetic marker to identify unknown Phyllanthus species. The nuclear region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on GenBank and BoldSystems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and BoldSystems while P. amarus Schum and Thonn, P. muellerianus (O. Ktze) Excel and P. urinaria Linn. were clearly compatible with other works. The sequence data were analyzed and classified with tree-based analyses of Mr.Bayes 3.2.1. in order to reveal their phylogenetic relationship. Results of the nucleotide sequences and fragment analysis were published on BoldSystems for barcoding as non-coding marker translation matrix.
| Published in | International Journal of Genetics and Genomics (Volume 3, Issue 1) |
| DOI | 10.11648/j.ijgg.20150301.11 |
| Page(s) | 1-7 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Phyllanthus species, DNA Extraction, PCR Amplification, Nucleotide Sequences, Gen Bank, Bold Systems, ITS, Molecular Phylogenetics
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APA Style
Daniel Azubuike Awomukwu, Bio Louis Nyananyo, Paula Spies, Bulelani Londoloza Sizani. (2015). Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication. International Journal of Genetics and Genomics, 3(1), 1-7. https://doi.org/10.11648/j.ijgg.20150301.11
ACS Style
Daniel Azubuike Awomukwu; Bio Louis Nyananyo; Paula Spies; Bulelani Londoloza Sizani. Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication. Int. J. Genet. Genomics 2015, 3(1), 1-7. doi: 10.11648/j.ijgg.20150301.11
AMA Style
Daniel Azubuike Awomukwu, Bio Louis Nyananyo, Paula Spies, Bulelani Londoloza Sizani. Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication. Int J Genet Genomics. 2015;3(1):1-7. doi: 10.11648/j.ijgg.20150301.11
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Download@article{10.11648/j.ijgg.20150301.11,
author = {Daniel Azubuike Awomukwu and Bio Louis Nyananyo and Paula Spies and Bulelani Londoloza Sizani},
title = {Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication},
journal = {International Journal of Genetics and Genomics},
volume = {3},
number = {1},
pages = {1-7},
doi = {10.11648/j.ijgg.20150301.11},
url = {https://doi.org/10.11648/j.ijgg.20150301.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20150301.11},
abstract = {An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Transcribed Spacer (ITS 4-5) genetic marker to identify unknown Phyllanthus species. The nuclear region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on GenBank and BoldSystems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and BoldSystems while P. amarus Schum and Thonn, P. muellerianus (O. Ktze) Excel and P. urinaria Linn. were clearly compatible with other works. The sequence data were analyzed and classified with tree-based analyses of Mr.Bayes 3.2.1. in order to reveal their phylogenetic relationship. Results of the nucleotide sequences and fragment analysis were published on BoldSystems for barcoding as non-coding marker translation matrix.},
year = {2015}
}
TY - JOUR T1 - Identification, Validation and Classification of the Genus Phyllanthus in Nigeria Using ITS Genetic Marker and the Taxonomic Implication AU - Daniel Azubuike Awomukwu AU - Bio Louis Nyananyo AU - Paula Spies AU - Bulelani Londoloza Sizani Y1 - 2015/02/16 PY - 2015 N1 - https://doi.org/10.11648/j.ijgg.20150301.11 DO - 10.11648/j.ijgg.20150301.11 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 1 EP - 7 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20150301.11 AB - An extraction, purification, PCR amplification and sequencing of DNA from five species of Phyllanthus in Nigeria namely P. amarus Schum and Thonn, P. urinaria Linn., P. odontadenius Mull-Arg., P. niruroides Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were carried out using nuclear ribosomal Internal Transcribed Spacer (ITS 4-5) genetic marker to identify unknown Phyllanthus species. The nuclear region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on GenBank and BoldSystems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and BoldSystems while P. amarus Schum and Thonn, P. muellerianus (O. Ktze) Excel and P. urinaria Linn. were clearly compatible with other works. The sequence data were analyzed and classified with tree-based analyses of Mr.Bayes 3.2.1. in order to reveal their phylogenetic relationship. Results of the nucleotide sequences and fragment analysis were published on BoldSystems for barcoding as non-coding marker translation matrix. VL - 3 IS - 1 ER -
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