Helicobacter pylori play an essential role in the pathogenesis of upper gastrointestinal disorders. The diagnostic role of the bacterium thus has been a subject of intense investigations. In this study we used an immune-chromatographic method and the polymerase chain reaction (PCR) to detect H. pylori in the saliva of patients with clinically diagnosed upper gastrointestinal disorders. Thirty such patients reporting to the Korle-Bu Teaching Hospital (Accra, Ghana) for upper gastrointestinal endoscopy consented for this study. Saliva samples were collected from each subject and analysed for H. pylori antibodies using a rapid immuno-chromatographic assay and H. pylori DNA by nested PCR using specific primers. Ten (33.3%) out of the 30 samples tested positive for the saliva antibody test with the most prevalent gastrointestinal disorders among the positive subjects being peptic ulcer (60%) followed by gastritis (30%) and esophagitis (10%). Following nested PCR analysis, a 346bp fragment of the vacA (m2) gene region of H. pylori was amplified in 9 (90%) out the 10 samples that were positive by the rapid immuno-chromatographic assay. Saliva samples could serve as a reliable non-invasive alternative to detect the presence of H. pylori infection in synergy with available diagnostic methods in Ghana.
| Published in | International Journal of Genetics and Genomics (Volume 2, Issue 5) |
| DOI | 10.11648/j.ijgg.20140205.11 |
| Page(s) | 80-83 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2014. Published by Science Publishing Group |
Helicobacter Pylori, Saliva, Polymerase Chain Reaction
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APA Style
Richard H. Asmah, Timothy Archampong, Charles A. Brown, Samuel B. Ntiamoah, Ebenezer K. Aidoo, et al. (2014). Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana. International Journal of Genetics and Genomics, 2(5), 80-83. https://doi.org/10.11648/j.ijgg.20140205.11
ACS Style
Richard H. Asmah; Timothy Archampong; Charles A. Brown; Samuel B. Ntiamoah; Ebenezer K. Aidoo, et al. Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana. Int. J. Genet. Genomics 2014, 2(5), 80-83. doi: 10.11648/j.ijgg.20140205.11
AMA Style
Richard H. Asmah, Timothy Archampong, Charles A. Brown, Samuel B. Ntiamoah, Ebenezer K. Aidoo, et al. Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana. Int J Genet Genomics. 2014;2(5):80-83. doi: 10.11648/j.ijgg.20140205.11
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Download@article{10.11648/j.ijgg.20140205.11,
author = {Richard H. Asmah and Timothy Archampong and Charles A. Brown and Samuel B. Ntiamoah and Ebenezer K. Aidoo and Richard Gyasi and Edwin K. Wiredu},
title = {Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana},
journal = {International Journal of Genetics and Genomics},
volume = {2},
number = {5},
pages = {80-83},
doi = {10.11648/j.ijgg.20140205.11},
url = {https://doi.org/10.11648/j.ijgg.20140205.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20140205.11},
abstract = {Helicobacter pylori play an essential role in the pathogenesis of upper gastrointestinal disorders. The diagnostic role of the bacterium thus has been a subject of intense investigations. In this study we used an immune-chromatographic method and the polymerase chain reaction (PCR) to detect H. pylori in the saliva of patients with clinically diagnosed upper gastrointestinal disorders. Thirty such patients reporting to the Korle-Bu Teaching Hospital (Accra, Ghana) for upper gastrointestinal endoscopy consented for this study. Saliva samples were collected from each subject and analysed for H. pylori antibodies using a rapid immuno-chromatographic assay and H. pylori DNA by nested PCR using specific primers. Ten (33.3%) out of the 30 samples tested positive for the saliva antibody test with the most prevalent gastrointestinal disorders among the positive subjects being peptic ulcer (60%) followed by gastritis (30%) and esophagitis (10%). Following nested PCR analysis, a 346bp fragment of the vacA (m2) gene region of H. pylori was amplified in 9 (90%) out the 10 samples that were positive by the rapid immuno-chromatographic assay. Saliva samples could serve as a reliable non-invasive alternative to detect the presence of H. pylori infection in synergy with available diagnostic methods in Ghana.},
year = {2014}
}
TY - JOUR T1 - Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana AU - Richard H. Asmah AU - Timothy Archampong AU - Charles A. Brown AU - Samuel B. Ntiamoah AU - Ebenezer K. Aidoo AU - Richard Gyasi AU - Edwin K. Wiredu Y1 - 2014/11/10 PY - 2014 N1 - https://doi.org/10.11648/j.ijgg.20140205.11 DO - 10.11648/j.ijgg.20140205.11 T2 - International Journal of Genetics and Genomics JF - International Journal of Genetics and Genomics JO - International Journal of Genetics and Genomics SP - 80 EP - 83 PB - Science Publishing Group SN - 2376-7359 UR - https://doi.org/10.11648/j.ijgg.20140205.11 AB - Helicobacter pylori play an essential role in the pathogenesis of upper gastrointestinal disorders. The diagnostic role of the bacterium thus has been a subject of intense investigations. In this study we used an immune-chromatographic method and the polymerase chain reaction (PCR) to detect H. pylori in the saliva of patients with clinically diagnosed upper gastrointestinal disorders. Thirty such patients reporting to the Korle-Bu Teaching Hospital (Accra, Ghana) for upper gastrointestinal endoscopy consented for this study. Saliva samples were collected from each subject and analysed for H. pylori antibodies using a rapid immuno-chromatographic assay and H. pylori DNA by nested PCR using specific primers. Ten (33.3%) out of the 30 samples tested positive for the saliva antibody test with the most prevalent gastrointestinal disorders among the positive subjects being peptic ulcer (60%) followed by gastritis (30%) and esophagitis (10%). Following nested PCR analysis, a 346bp fragment of the vacA (m2) gene region of H. pylori was amplified in 9 (90%) out the 10 samples that were positive by the rapid immuno-chromatographic assay. Saliva samples could serve as a reliable non-invasive alternative to detect the presence of H. pylori infection in synergy with available diagnostic methods in Ghana. VL - 2 IS - 5 ER -
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