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Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana

Received: 18 June 2014     Accepted: 9 July 2014     Published: 10 November 2014
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Abstract

Helicobacter pylori play an essential role in the pathogenesis of upper gastrointestinal disorders. The diagnostic role of the bacterium thus has been a subject of intense investigations. In this study we used an immune-chromatographic method and the polymerase chain reaction (PCR) to detect H. pylori in the saliva of patients with clinically diagnosed upper gastrointestinal disorders. Thirty such patients reporting to the Korle-Bu Teaching Hospital (Accra, Ghana) for upper gastrointestinal endoscopy consented for this study. Saliva samples were collected from each subject and analysed for H. pylori antibodies using a rapid immuno-chromatographic assay and H. pylori DNA by nested PCR using specific primers. Ten (33.3%) out of the 30 samples tested positive for the saliva antibody test with the most prevalent gastrointestinal disorders among the positive subjects being peptic ulcer (60%) followed by gastritis (30%) and esophagitis (10%). Following nested PCR analysis, a 346bp fragment of the vacA (m2) gene region of H. pylori was amplified in 9 (90%) out the 10 samples that were positive by the rapid immuno-chromatographic assay. Saliva samples could serve as a reliable non-invasive alternative to detect the presence of H. pylori infection in synergy with available diagnostic methods in Ghana.

Published in International Journal of Genetics and Genomics (Volume 2, Issue 5)
DOI 10.11648/j.ijgg.20140205.11
Page(s) 80-83
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2014. Published by Science Publishing Group

Keywords

Helicobacter Pylori, Saliva, Polymerase Chain Reaction

References
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Cite This Article
  • APA Style

    Richard H. Asmah, Timothy Archampong, Charles A. Brown, Samuel B. Ntiamoah, Ebenezer K. Aidoo, et al. (2014). Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana. International Journal of Genetics and Genomics, 2(5), 80-83. https://doi.org/10.11648/j.ijgg.20140205.11

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    ACS Style

    Richard H. Asmah; Timothy Archampong; Charles A. Brown; Samuel B. Ntiamoah; Ebenezer K. Aidoo, et al. Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana. Int. J. Genet. Genomics 2014, 2(5), 80-83. doi: 10.11648/j.ijgg.20140205.11

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    AMA Style

    Richard H. Asmah, Timothy Archampong, Charles A. Brown, Samuel B. Ntiamoah, Ebenezer K. Aidoo, et al. Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana. Int J Genet Genomics. 2014;2(5):80-83. doi: 10.11648/j.ijgg.20140205.11

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  • @article{10.11648/j.ijgg.20140205.11,
      author = {Richard H. Asmah and Timothy Archampong and Charles A. Brown and Samuel B. Ntiamoah and Ebenezer K. Aidoo and Richard Gyasi and Edwin K. Wiredu},
      title = {Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana},
      journal = {International Journal of Genetics and Genomics},
      volume = {2},
      number = {5},
      pages = {80-83},
      doi = {10.11648/j.ijgg.20140205.11},
      url = {https://doi.org/10.11648/j.ijgg.20140205.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijgg.20140205.11},
      abstract = {Helicobacter pylori play an essential role in the pathogenesis of upper gastrointestinal disorders. The diagnostic role of the bacterium thus has been a subject of intense investigations. In this study we used an immune-chromatographic method and the polymerase chain reaction (PCR) to detect H. pylori in the saliva of patients with clinically diagnosed upper gastrointestinal disorders. Thirty such patients reporting to the Korle-Bu Teaching Hospital (Accra, Ghana) for upper gastrointestinal endoscopy consented for this study. Saliva samples were collected from each subject and analysed for H. pylori antibodies using a rapid immuno-chromatographic assay and H. pylori DNA by nested PCR using specific primers. Ten (33.3%) out of the 30 samples tested positive for the saliva antibody test with the most prevalent gastrointestinal disorders among the positive subjects being peptic ulcer (60%) followed by gastritis (30%) and esophagitis (10%). Following nested PCR analysis, a 346bp fragment of the vacA (m2) gene region of H. pylori was amplified in 9 (90%) out the 10 samples that were positive by the rapid immuno-chromatographic assay. Saliva samples could serve as a reliable non-invasive alternative to detect the presence of H. pylori infection in synergy with available diagnostic methods in Ghana.},
     year = {2014}
    }
    

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    T1  - Helicobacter Pylori vacA Gene Detection in Saliva of Patients with Upper Gastrointestinal Disorders in Accra, Ghana
    AU  - Richard H. Asmah
    AU  - Timothy Archampong
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    AB  - Helicobacter pylori play an essential role in the pathogenesis of upper gastrointestinal disorders. The diagnostic role of the bacterium thus has been a subject of intense investigations. In this study we used an immune-chromatographic method and the polymerase chain reaction (PCR) to detect H. pylori in the saliva of patients with clinically diagnosed upper gastrointestinal disorders. Thirty such patients reporting to the Korle-Bu Teaching Hospital (Accra, Ghana) for upper gastrointestinal endoscopy consented for this study. Saliva samples were collected from each subject and analysed for H. pylori antibodies using a rapid immuno-chromatographic assay and H. pylori DNA by nested PCR using specific primers. Ten (33.3%) out of the 30 samples tested positive for the saliva antibody test with the most prevalent gastrointestinal disorders among the positive subjects being peptic ulcer (60%) followed by gastritis (30%) and esophagitis (10%). Following nested PCR analysis, a 346bp fragment of the vacA (m2) gene region of H. pylori was amplified in 9 (90%) out the 10 samples that were positive by the rapid immuno-chromatographic assay. Saliva samples could serve as a reliable non-invasive alternative to detect the presence of H. pylori infection in synergy with available diagnostic methods in Ghana.
    VL  - 2
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Author Information
  • Dept. of Medical Laboratory Sciences, University of Ghana School of Allied Health Science, Accra, Ghana

  • Dept. of Medicine, University of Ghana Medical School, Accra, Ghana

  • Dept. of Medical Laboratory Sciences, University of Ghana School of Allied Health Science, Accra, Ghana

  • Dept. of Medical Laboratory Sciences, University of Ghana School of Allied Health Science, Accra, Ghana

  • Dept. of Microbiology, University of Ghana Medical School, Accra, Ghana

  • Dept. of Pathology, University of Ghana Medical School, Accra, Ghana

  • Dept. of Medical Laboratory Sciences, University of Ghana School of Allied Health Science, Accra, Ghana

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