Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins

Kewen Zheng

doi:doi:10.11648/j.ijcu.20190302.14

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Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins

Kewen Zheng

Published in International Journal of Clinical Urology (Volume 3, Issue 2)

Received: 15 October 2019 Accepted: 6 November 2019 Published: 12 November 2019

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Abstract

Background The main component of realgar is arsenic disulfide (As2S2). Modern medical research shows that realgar can inhibit tumorigenesis and development of tumor by regulating cell proliferation and apoptosis. However, the lack of the traditional realgar preparations present with lower bioavailability, which further affects the therapeutic effect. With the improvement of new drug preparation processes, nano-androgen is increasingly used for anti-tumor drugs. Objective In order to investigate the effect of nano-realgar on proliferation of bladder cancer cell line RT4, we performed the research. Methods Nano-realgar was prepared by mechanical milling method. RT4 cell was used as target cell. MTT assay was used to detect the proliferation of RT4 cell treated with nano-realgar. And western blot was used to detect the expression of apoptosis-related proteins (Bax, Caspase-3) in the RT4 cell treated with nano-realgar. Results Nano-realgar could significantly inhibit the proliferation of RT4 cell. After treated with 10 ug/ml, 20 ug/ml and 40 ug/ml nano-realgar for 24 h and 48 h, the proliferation inhibition rate of RT4 cell increased significantly compared with the negative control group (P<0.05), and the expression of apoptosis-related proteins, Bad and Caspase-3, increased significantly after treated with 20 ug/ml and 40 ug/ml nano-realgar for 48 h (P<0.05). Conclusion Nano-realgar can inhibit the proliferation of RT4 cell, and induces the expression of Bax and Caspase-3.

Published in	International Journal of Clinical Urology (Volume 3, Issue 2)
DOI	10.11648/j.ijcu.20190302.14
Page(s)	46-49
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Proliferation, Nano-realgar, Bax, Caspase-3, Bladder Cancer Cell

References

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APA Style

Kewen Zheng. (2019). Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins. International Journal of Clinical Urology, 3(2), 46-49. https://doi.org/10.11648/j.ijcu.20190302.14

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ACS Style

Kewen Zheng. Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins. Int. J. Clin. Urol. 2019, 3(2), 46-49. doi: 10.11648/j.ijcu.20190302.14

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AMA Style

Kewen Zheng. Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins. Int J Clin Urol. 2019;3(2):46-49. doi: 10.11648/j.ijcu.20190302.14

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@article{10.11648/j.ijcu.20190302.14,
  author = {Kewen Zheng},
  title = {Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins},
  journal = {International Journal of Clinical Urology},
  volume = {3},
  number = {2},
  pages = {46-49},
  doi = {10.11648/j.ijcu.20190302.14},
  url = {https://doi.org/10.11648/j.ijcu.20190302.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcu.20190302.14},
  abstract = {Background The main component of realgar is arsenic disulfide (As2S2). Modern medical research shows that realgar can inhibit tumorigenesis and development of tumor by regulating cell proliferation and apoptosis. However, the lack of the traditional realgar preparations present with lower bioavailability, which further affects the therapeutic effect. With the improvement of new drug preparation processes, nano-androgen is increasingly used for anti-tumor drugs. Objective In order to investigate the effect of nano-realgar on proliferation of bladder cancer cell line RT4, we performed the research. Methods Nano-realgar was prepared by mechanical milling method. RT4 cell was used as target cell. MTT assay was used to detect the proliferation of RT4 cell treated with nano-realgar. And western blot was used to detect the expression of apoptosis-related proteins (Bax, Caspase-3) in the RT4 cell treated with nano-realgar. Results Nano-realgar could significantly inhibit the proliferation of RT4 cell. After treated with 10 ug/ml, 20 ug/ml and 40 ug/ml nano-realgar for 24 h and 48 h, the proliferation inhibition rate of RT4 cell increased significantly compared with the negative control group (P<0.05), and the expression of apoptosis-related proteins, Bad and Caspase-3, increased significantly after treated with 20 ug/ml and 40 ug/ml nano-realgar for 48 h (P<0.05). Conclusion Nano-realgar can inhibit the proliferation of RT4 cell, and induces the expression of Bax and Caspase-3.},
 year = {2019}
}

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TY - JOUR
T1 - Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins
AU - Kewen Zheng
Y1 - 2019/11/12
PY - 2019
N1 - https://doi.org/10.11648/j.ijcu.20190302.14
DO - 10.11648/j.ijcu.20190302.14
T2 - International Journal of Clinical Urology
JF - International Journal of Clinical Urology
JO - International Journal of Clinical Urology
SP - 46
EP - 49
PB - Science Publishing Group
SN - 2640-1355
UR - https://doi.org/10.11648/j.ijcu.20190302.14
AB - Background The main component of realgar is arsenic disulfide (As2S2). Modern medical research shows that realgar can inhibit tumorigenesis and development of tumor by regulating cell proliferation and apoptosis. However, the lack of the traditional realgar preparations present with lower bioavailability, which further affects the therapeutic effect. With the improvement of new drug preparation processes, nano-androgen is increasingly used for anti-tumor drugs. Objective In order to investigate the effect of nano-realgar on proliferation of bladder cancer cell line RT4, we performed the research. Methods Nano-realgar was prepared by mechanical milling method. RT4 cell was used as target cell. MTT assay was used to detect the proliferation of RT4 cell treated with nano-realgar. And western blot was used to detect the expression of apoptosis-related proteins (Bax, Caspase-3) in the RT4 cell treated with nano-realgar. Results Nano-realgar could significantly inhibit the proliferation of RT4 cell. After treated with 10 ug/ml, 20 ug/ml and 40 ug/ml nano-realgar for 24 h and 48 h, the proliferation inhibition rate of RT4 cell increased significantly compared with the negative control group (P<0.05), and the expression of apoptosis-related proteins, Bad and Caspase-3, increased significantly after treated with 20 ug/ml and 40 ug/ml nano-realgar for 48 h (P<0.05). Conclusion Nano-realgar can inhibit the proliferation of RT4 cell, and induces the expression of Bax and Caspase-3.
VL - 3
IS - 2
ER -

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Kewen Zheng

Department of Urology, The First Affiliated Hospital of Wenzhou Medical University, The First Clinical College of Wenzhou Medical University, Wenzhou City, R. P. China

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APA Style

Kewen Zheng. (2019). Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins. International Journal of Clinical Urology, 3(2), 46-49. https://doi.org/10.11648/j.ijcu.20190302.14

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ACS Style

Kewen Zheng. Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins. Int. J. Clin. Urol. 2019, 3(2), 46-49. doi: 10.11648/j.ijcu.20190302.14

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AMA Style

Kewen Zheng. Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins. Int J Clin Urol. 2019;3(2):46-49. doi: 10.11648/j.ijcu.20190302.14

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@article{10.11648/j.ijcu.20190302.14,
  author = {Kewen Zheng},
  title = {Nano-realgar Inhibits the Proliferation of Bladder Cancer Cell and Induces the Expression of Apoptosis-related Proteins},
  journal = {International Journal of Clinical Urology},
  volume = {3},
  number = {2},
  pages = {46-49},
  doi = {10.11648/j.ijcu.20190302.14},
  url = {https://doi.org/10.11648/j.ijcu.20190302.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcu.20190302.14},
  abstract = {Background The main component of realgar is arsenic disulfide (As2S2). Modern medical research shows that realgar can inhibit tumorigenesis and development of tumor by regulating cell proliferation and apoptosis. However, the lack of the traditional realgar preparations present with lower bioavailability, which further affects the therapeutic effect. With the improvement of new drug preparation processes, nano-androgen is increasingly used for anti-tumor drugs. Objective In order to investigate the effect of nano-realgar on proliferation of bladder cancer cell line RT4, we performed the research. Methods Nano-realgar was prepared by mechanical milling method. RT4 cell was used as target cell. MTT assay was used to detect the proliferation of RT4 cell treated with nano-realgar. And western blot was used to detect the expression of apoptosis-related proteins (Bax, Caspase-3) in the RT4 cell treated with nano-realgar. Results Nano-realgar could significantly inhibit the proliferation of RT4 cell. After treated with 10 ug/ml, 20 ug/ml and 40 ug/ml nano-realgar for 24 h and 48 h, the proliferation inhibition rate of RT4 cell increased significantly compared with the negative control group (P<0.05), and the expression of apoptosis-related proteins, Bad and Caspase-3, increased significantly after treated with 20 ug/ml and 40 ug/ml nano-realgar for 48 h (P<0.05). Conclusion Nano-realgar can inhibit the proliferation of RT4 cell, and induces the expression of Bax and Caspase-3.},
 year = {2019}
}

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