Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters

Detlef Schikora; Michael Weber

doi:doi:10.11648/j.ijcocr.20190405.11

| Peer-Reviewed

Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters

Detlef Schikora, Michael Weber

Published in International Journal of Clinical Oncology and Cancer Research (Volume 4, Issue 5)

Received: 8 October 2019 Accepted: 6 November 2019 Published: 13 November 2019

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

We demonstrate a new diagnostic method, the Photodynamic Infrared Spectroscoppy (PDIS), which is able to detect circulating tumor clusters and circulating tumor cells in the circulatory system. In particular the circulating tumor clusters are agreed as the main cause for metastases formation and therefore for the cancer mortality. The cancer mortality rate is unchanged worldwide in the last twenty years, indicating the absence of clinically effective metastases prevention therapies. No diagnostic method is existing, which allows to detect tumor clusters in vivo in the blood, which is the prerequisite for tumor cluster destruction and metastases prevention. The PDIS method is based on photodynamic physics and high resolution spectroscopy and is using calibrated spectroscopic data for the diagnostic analysis of the blood screening spectra. In the paper we are focussing to brest cancer diagnostics using the photosensitizer Indocyangreen. A flow appartus is described, which enables to calibrate the fluorescence spectra of single tumor cells and single tumor clusters. Due to the calibrated diagnostic data, the PDIS enables the identification of circulating tumot cells and tumor clusters in the bloodstream with the ultimate accuracy of one cluster per 6 l blood and with a sensitivity of 98%. Circulating tumor cell clusters are formed only in solid tumors, that’s why they are appropriate objects to validate cancer treatments and to recognice cancer formation. Due to the one-to one correspondence between tumor clusters in blood and the existence of solid tumors in the organism, the PDIS as diagnostic method can be used to control and to verify any oncologic treatment with respect to its clinical efficay. The common "wait and hope" strategy after finishing a chemotheraypy can be replaced by an effective follow-up strategy. Furthermore, the PDIS diagnostics can be used to recognize a tumor formation in the earliest possible stage, the carcinom in-situ stage. Finally the in-vivo detection of tumor clusters in the blood enables the immediate and controlled destruction of the these clusters by Photodynamic Therapy or other oncologic methods and therefore an increase of the overall survival of cancer patients and a decrease of the cancer mortalitay rate.

Published in	International Journal of Clinical Oncology and Cancer Research (Volume 4, Issue 5)
DOI	10.11648/j.ijcocr.20190405.11
Page(s)	38-44
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Photodynamic Diagnosis, Circulating Tumor Cell Clusters, Fluorescence Spectroscopy, High Resolution Blood Screening, Cancer Metastases Prevention, Cancer Treatments Verification

References

[1]	Massague J, Obenauf AC. Metastatic colonization by circulating tumour cells. Nature. 2016; 529 (7586): 298–306. doi: 10.1038/nature17038.
[2]	Anna Fabisiewicz, Ewa Grzybowska CTC clusters in cancer progression and metastasis Med Oncol (2017) 34: 12 DOI 10.1007/s12032-016-0875-0.
[3]	Watanabe S. The metastasizability of tumor cells. Cancer. 1954; 7 (2): 215–23.
[4]	Aceto N, Bardia A, Miyamoto DT, Donaldson MC, Wittner BS, Spencer JA, et al. Circulating tumor cell clusters are oligoclonal precursors of breast cancer metastasis. Cell. 2014; 158 (5): 1110–064. 22. doi: 10.1016/j.cell.2014.07.013.
[5]	Aceto N, Toner M, Maheswaran S, Haber DA. En route to metastasis: circulating tumor cell clusters and epithelial-to-mes- enchymal transition. Trends Cancer. 2015; 1 (1): 44–52. doi: 10.1016/j.trecan.2015.07.006.
[6]	Hong Y, Fang F, Zhang Q. Circulating tumor cell clusters: what we know and what we expect (review). Int J Oncol. 2016;. doi: 10.3892/ijo.2016.3747.
[7]	Au SH, Storey BD, Moore JC, Tang Q, Chen YL, Javaid S, et al. Clusters of circulating tumor cells traverse capillary-sized vessels. Proc Natl Acad Sci USA. 2016; 113 (18): 4947–52. doi: 10.1073/pnas.1524448113.
[8]	Cheung KJ, Padmanaban V, Silvestri V, Schipper K, Cohen JD, Fairchild AN, et al. Polyclonal breast cancer metastases arise from collective dissemination of keratin 14-expressing tumor cell clusters. Proc Natl Acad Sci USA. 2016; 113 (7): E854–63. doi: 10.1073/pnas.1508541113.
[9]	Szczerba et al. "Neutrophils escort circulating tumr cells to enable cell cycle progression". 2019 Nature, 37-39.
[10]	Gkountela S. (2019), Cell 176, 98-112.
[11]	Jablonski A., Efficiency of anti-Stokes fluorescence in dyes. In: Nature. Band 131, (1933), S. 839–840.
[12]	Cristofanilli M, Budd GT, Ellis MJ, Stopeck A, Matera J, Miller MC, et. al. Circulating tumor cells, diseases progression and survival in metastatic breast cancer NEngl J Med 2004: 351 (8): 781-791.
[13]	Bäumler W, Abels C, Karrer S, Weiß T, Messmann H, Landthaler M, Szeimies R Photo-oxidative killing of human colonic cancer cells using indocyanine green and infrared light Br J Cancer (1999) May; 80 (3-4): 360-363.
[14]	D. Schikora and M. Weber, British Journal of Cancer, submission#TH-2019-4280.

Cite This Article

Plain Text BibTeX RIS

APA Style

Detlef Schikora, Michael Weber. (2019). Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters. International Journal of Clinical Oncology and Cancer Research, 4(5), 38-44. https://doi.org/10.11648/j.ijcocr.20190405.11

Copy | Download

ACS Style

Detlef Schikora; Michael Weber. Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters. Int. J. Clin. Oncol. Cancer Res. 2019, 4(5), 38-44. doi: 10.11648/j.ijcocr.20190405.11

Copy | Download

AMA Style

Detlef Schikora, Michael Weber. Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters. Int J Clin Oncol Cancer Res. 2019;4(5):38-44. doi: 10.11648/j.ijcocr.20190405.11

Copy | Download

@article{10.11648/j.ijcocr.20190405.11,
  author = {Detlef Schikora and Michael Weber},
  title = {Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {4},
  number = {5},
  pages = {38-44},
  doi = {10.11648/j.ijcocr.20190405.11},
  url = {https://doi.org/10.11648/j.ijcocr.20190405.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20190405.11},
  abstract = {We demonstrate a new diagnostic method, the Photodynamic Infrared Spectroscoppy (PDIS), which is able to detect circulating tumor clusters and circulating tumor cells in the circulatory system. In particular the circulating tumor clusters are agreed as the main cause for metastases formation and therefore for the cancer mortality. The cancer mortality rate is unchanged worldwide in the last twenty years, indicating the absence of clinically effective metastases prevention therapies. No diagnostic method is existing, which allows to detect tumor clusters in vivo in the blood, which is the prerequisite for tumor cluster destruction and metastases prevention. The PDIS method is based on photodynamic physics and high resolution spectroscopy and is using calibrated spectroscopic data for the diagnostic analysis of the blood screening spectra. In the paper we are focussing to brest cancer diagnostics using the photosensitizer Indocyangreen. A flow appartus is described, which enables to calibrate the fluorescence spectra of single tumor cells and single tumor clusters. Due to the calibrated diagnostic data, the PDIS enables the identification of circulating tumot cells and tumor clusters in the bloodstream with the ultimate accuracy of one cluster per 6 l blood and with a sensitivity of 98%. Circulating tumor cell clusters are formed only in solid tumors, that’s why they are appropriate objects to validate cancer treatments and to recognice cancer formation. Due to the one-to one correspondence between tumor clusters in blood and the existence of solid tumors in the organism, the PDIS as diagnostic method can be used to control and to verify any oncologic treatment with respect to its clinical efficay. The common "wait and hope" strategy after finishing a chemotheraypy can be replaced by an effective follow-up strategy. Furthermore, the PDIS diagnostics can be used to recognize a tumor formation in the earliest possible stage, the carcinom in-situ stage. Finally the in-vivo detection of tumor clusters in the blood enables the immediate and controlled destruction of the these clusters by Photodynamic Therapy or other oncologic methods and therefore an increase of the overall survival of cancer patients and a decrease of the cancer mortalitay rate.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters
AU  - Detlef Schikora
AU  - Michael Weber
Y1  - 2019/11/13
PY  - 2019
N1  - https://doi.org/10.11648/j.ijcocr.20190405.11
DO  - 10.11648/j.ijcocr.20190405.11
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20190405.11
AB  - We demonstrate a new diagnostic method, the Photodynamic Infrared Spectroscoppy (PDIS), which is able to detect circulating tumor clusters and circulating tumor cells in the circulatory system. In particular the circulating tumor clusters are agreed as the main cause for metastases formation and therefore for the cancer mortality. The cancer mortality rate is unchanged worldwide in the last twenty years, indicating the absence of clinically effective metastases prevention therapies. No diagnostic method is existing, which allows to detect tumor clusters in vivo in the blood, which is the prerequisite for tumor cluster destruction and metastases prevention. The PDIS method is based on photodynamic physics and high resolution spectroscopy and is using calibrated spectroscopic data for the diagnostic analysis of the blood screening spectra. In the paper we are focussing to brest cancer diagnostics using the photosensitizer Indocyangreen. A flow appartus is described, which enables to calibrate the fluorescence spectra of single tumor cells and single tumor clusters. Due to the calibrated diagnostic data, the PDIS enables the identification of circulating tumot cells and tumor clusters in the bloodstream with the ultimate accuracy of one cluster per 6 l blood and with a sensitivity of 98%. Circulating tumor cell clusters are formed only in solid tumors, that’s why they are appropriate objects to validate cancer treatments and to recognice cancer formation. Due to the one-to one correspondence between tumor clusters in blood and the existence of solid tumors in the organism, the PDIS as diagnostic method can be used to control and to verify any oncologic treatment with respect to its clinical efficay. The common "wait and hope" strategy after finishing a chemotheraypy can be replaced by an effective follow-up strategy. Furthermore, the PDIS diagnostics can be used to recognize a tumor formation in the earliest possible stage, the carcinom in-situ stage. Finally the in-vivo detection of tumor clusters in the blood enables the immediate and controlled destruction of the these clusters by Photodynamic Therapy or other oncologic methods and therefore an increase of the overall survival of cancer patients and a decrease of the cancer mortalitay rate.
VL  - 4
IS  - 5
ER  -

Copy | Download

Author Information

Detlef Schikora

Department of Biophysics, Faculty of Science, University of Paderborn, Paderborn, Germany
Michael Weber

Laser Clinic Lauenfoerde, Cancer Division, Lauenfoerde, Germany

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Detlef Schikora, Michael Weber. (2019). Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters. International Journal of Clinical Oncology and Cancer Research, 4(5), 38-44. https://doi.org/10.11648/j.ijcocr.20190405.11

Copy | Download

ACS Style

Detlef Schikora; Michael Weber. Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters. Int. J. Clin. Oncol. Cancer Res. 2019, 4(5), 38-44. doi: 10.11648/j.ijcocr.20190405.11

Copy | Download

AMA Style

Detlef Schikora, Michael Weber. Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters. Int J Clin Oncol Cancer Res. 2019;4(5):38-44. doi: 10.11648/j.ijcocr.20190405.11

Copy | Download

@article{10.11648/j.ijcocr.20190405.11,
  author = {Detlef Schikora and Michael Weber},
  title = {Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {4},
  number = {5},
  pages = {38-44},
  doi = {10.11648/j.ijcocr.20190405.11},
  url = {https://doi.org/10.11648/j.ijcocr.20190405.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20190405.11},
  abstract = {We demonstrate a new diagnostic method, the Photodynamic Infrared Spectroscoppy (PDIS), which is able to detect circulating tumor clusters and circulating tumor cells in the circulatory system. In particular the circulating tumor clusters are agreed as the main cause for metastases formation and therefore for the cancer mortality. The cancer mortality rate is unchanged worldwide in the last twenty years, indicating the absence of clinically effective metastases prevention therapies. No diagnostic method is existing, which allows to detect tumor clusters in vivo in the blood, which is the prerequisite for tumor cluster destruction and metastases prevention. The PDIS method is based on photodynamic physics and high resolution spectroscopy and is using calibrated spectroscopic data for the diagnostic analysis of the blood screening spectra. In the paper we are focussing to brest cancer diagnostics using the photosensitizer Indocyangreen. A flow appartus is described, which enables to calibrate the fluorescence spectra of single tumor cells and single tumor clusters. Due to the calibrated diagnostic data, the PDIS enables the identification of circulating tumot cells and tumor clusters in the bloodstream with the ultimate accuracy of one cluster per 6 l blood and with a sensitivity of 98%. Circulating tumor cell clusters are formed only in solid tumors, that’s why they are appropriate objects to validate cancer treatments and to recognice cancer formation. Due to the one-to one correspondence between tumor clusters in blood and the existence of solid tumors in the organism, the PDIS as diagnostic method can be used to control and to verify any oncologic treatment with respect to its clinical efficay. The common "wait and hope" strategy after finishing a chemotheraypy can be replaced by an effective follow-up strategy. Furthermore, the PDIS diagnostics can be used to recognize a tumor formation in the earliest possible stage, the carcinom in-situ stage. Finally the in-vivo detection of tumor clusters in the blood enables the immediate and controlled destruction of the these clusters by Photodynamic Therapy or other oncologic methods and therefore an increase of the overall survival of cancer patients and a decrease of the cancer mortalitay rate.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Cancer Metastases Prevention by Photodynamic In-vivo Detection and Destruction of Circulating Tumor Cell Clusters
AU  - Detlef Schikora
AU  - Michael Weber
Y1  - 2019/11/13
PY  - 2019
N1  - https://doi.org/10.11648/j.ijcocr.20190405.11
DO  - 10.11648/j.ijcocr.20190405.11
T2  - International Journal of Clinical Oncology and Cancer Research
JF  - International Journal of Clinical Oncology and Cancer Research
JO  - International Journal of Clinical Oncology and Cancer Research
SP  - 38
EP  - 44
PB  - Science Publishing Group
SN  - 2578-9511
UR  - https://doi.org/10.11648/j.ijcocr.20190405.11
AB  - We demonstrate a new diagnostic method, the Photodynamic Infrared Spectroscoppy (PDIS), which is able to detect circulating tumor clusters and circulating tumor cells in the circulatory system. In particular the circulating tumor clusters are agreed as the main cause for metastases formation and therefore for the cancer mortality. The cancer mortality rate is unchanged worldwide in the last twenty years, indicating the absence of clinically effective metastases prevention therapies. No diagnostic method is existing, which allows to detect tumor clusters in vivo in the blood, which is the prerequisite for tumor cluster destruction and metastases prevention. The PDIS method is based on photodynamic physics and high resolution spectroscopy and is using calibrated spectroscopic data for the diagnostic analysis of the blood screening spectra. In the paper we are focussing to brest cancer diagnostics using the photosensitizer Indocyangreen. A flow appartus is described, which enables to calibrate the fluorescence spectra of single tumor cells and single tumor clusters. Due to the calibrated diagnostic data, the PDIS enables the identification of circulating tumot cells and tumor clusters in the bloodstream with the ultimate accuracy of one cluster per 6 l blood and with a sensitivity of 98%. Circulating tumor cell clusters are formed only in solid tumors, that’s why they are appropriate objects to validate cancer treatments and to recognice cancer formation. Due to the one-to one correspondence between tumor clusters in blood and the existence of solid tumors in the organism, the PDIS as diagnostic method can be used to control and to verify any oncologic treatment with respect to its clinical efficay. The common "wait and hope" strategy after finishing a chemotheraypy can be replaced by an effective follow-up strategy. Furthermore, the PDIS diagnostics can be used to recognize a tumor formation in the earliest possible stage, the carcinom in-situ stage. Finally the in-vivo detection of tumor clusters in the blood enables the immediate and controlled destruction of the these clusters by Photodynamic Therapy or other oncologic methods and therefore an increase of the overall survival of cancer patients and a decrease of the cancer mortalitay rate.
VL  - 4
IS  - 5
ER  -

Copy | Download