Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors

Mohammad Sahajadul Alam; M. Mizanur Rahman; Md. Monowar Hossain; Mohammed Abu Kawsar Sarker; Md. Monzurul Islam; Rokeya Begum; Most Umme Habiba; Gazi Nurun Nahar Sultana

doi:doi:10.11648/j.ijcocr.20180304.11

Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors

Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, Rokeya Begum, Most Umme Habiba, Gazi Nurun Nahar Sultana

Published in International Journal of Clinical Oncology and Cancer Research (Volume 3, Issue 4)

Received: 24 August 2018 Accepted: 14 September 2018 Published: 17 October 2018

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Abstract

Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.

Published in	International Journal of Clinical Oncology and Cancer Research (Volume 3, Issue 4)
DOI	10.11648/j.ijcocr.20180304.11
Page(s)	49-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Hmlh1 Gene Mutation, Gastric Cancer, Clinicopathological Relevance

References

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[2]	Orditura M, Galizia G, Sforza V, Gambardella V, Fabozzi A, Laterza MM, et al. Treatment of gastric cancer . World Journal of Gastroenterology. 2014 20 (7): 1635–49.
[3]	Cabebe EC, Mehta VK, Fisher G, Talavera F, Movsas M, McKenna R, et al. Gastric Cancer. Medscape Reference. WebMD. Archived from the original on 7 April 2014. Retrieved 4 April 2014.
[4]	Mathias U, Cheng Z, Sunjae L, Evelina S, Linn F, Gholamreza B, Rui B, Muhammad A, Zhengtao L (2017-08-18). A pathology atlas of the human cancer transcriptome. Science. 357 (6352):
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[7]	Smith MG, Hold GL, Tahara E & El-Omar EM. Cellular and molecular aspect of gastric cancer. World Journal of Gastroenterology 2006. 12: 2979–299.
[8]	Ong CAJ, Lao-Sirieix P & Fitzgerald. Biomarkers in Barrett's esophagus and esophageal adenocarcinoma: predictors of progression and prognosis. World Journal of Gastroenterology 2010. 16:5669–5681.
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[13]	Wenxian Z, Binshuang X, Lifeng W, Nong X, Qiong H, Yaping W, et. al. The MLH1 2101C>A (Q701K) variant increases the risk of gastric cancer in Chinese males. BMC Gastroenterology 2011. 11:133.
[14]	Available at https://ghr.nlm.nih.gov/gene/MLH1 accessed on 22.02.2018
[15]	Xian-Qiu X, Wei-Da G, Shi-Zhi W, Zheng-Dong Z, Xiao-Ping R, Feng R et.al. Polymorphisms of mismatch repair gene hMLH1 and hMSH2 and risk of gastric cancer in a Chinese population. Oncology Letters 2012. 3: 591-598.
[16]	Ping L, Xiao-Yong Z, Yun S, Dao-Fu Z. Microsatellite instability in gastric cancer and pre-cancerous Lesions. World Journal of Gastroenterology 2005; 11: 4904-4907.
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Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, et al. (2018). Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors. International Journal of Clinical Oncology and Cancer Research, 3(4), 49-54. https://doi.org/10.11648/j.ijcocr.20180304.11

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ACS Style

Mohammad Sahajadul Alam; M. Mizanur Rahman; Md. Monowar Hossain; Mohammed Abu Kawsar Sarker; Md. Monzurul Islam, et al. Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors. Int. J. Clin. Oncol. Cancer Res. 2018, 3(4), 49-54. doi: 10.11648/j.ijcocr.20180304.11

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AMA Style

Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, et al. Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors. Int J Clin Oncol Cancer Res. 2018;3(4):49-54. doi: 10.11648/j.ijcocr.20180304.11

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@article{10.11648/j.ijcocr.20180304.11,
  author = {Mohammad Sahajadul Alam and M. Mizanur Rahman and Md. Monowar Hossain and Mohammed Abu Kawsar Sarker and Md. Monzurul Islam and Rokeya Begum and Most Umme Habiba and Gazi Nurun Nahar Sultana},
  title = {Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {3},
  number = {4},
  pages = {49-54},
  doi = {10.11648/j.ijcocr.20180304.11},
  url = {https://doi.org/10.11648/j.ijcocr.20180304.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20180304.11},
  abstract = {Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.},
 year = {2018}
}

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TY - JOUR
T1 - Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors
AU - Mohammad Sahajadul Alam
AU - M. Mizanur Rahman
AU - Md. Monowar Hossain
AU - Mohammed Abu Kawsar Sarker
AU - Md. Monzurul Islam
AU - Rokeya Begum
AU - Most Umme Habiba
AU - Gazi Nurun Nahar Sultana
Y1 - 2018/10/17
PY - 2018
N1 - https://doi.org/10.11648/j.ijcocr.20180304.11
DO - 10.11648/j.ijcocr.20180304.11
T2 - International Journal of Clinical Oncology and Cancer Research
JF - International Journal of Clinical Oncology and Cancer Research
JO - International Journal of Clinical Oncology and Cancer Research
SP - 49
EP - 54
PB - Science Publishing Group
SN - 2578-9511
UR - https://doi.org/10.11648/j.ijcocr.20180304.11
AB - Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.
VL - 3
IS - 4
ER -

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Author Information

Mohammad Sahajadul Alam

Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
M. Mizanur Rahman

Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
Md. Monowar Hossain

Department of Surgical Oncology, Khulna Medical College, Khulna, Bangladesh
Mohammed Abu Kawsar Sarker

Department of Surgery, Aichi Medical College, Dhaka, Bangladesh
Md. Monzurul Islam

Department of Surgical Oncology, National Institute of Cancer Research and Hospital, Dhaka, Bangladesh
Rokeya Begum

Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Most Umme Habiba

Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh
Gazi Nurun Nahar Sultana

Centre for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka, Bangladesh

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Plain Text BibTeX RIS

APA Style

Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, et al. (2018). Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors. International Journal of Clinical Oncology and Cancer Research, 3(4), 49-54. https://doi.org/10.11648/j.ijcocr.20180304.11

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ACS Style

Mohammad Sahajadul Alam; M. Mizanur Rahman; Md. Monowar Hossain; Mohammed Abu Kawsar Sarker; Md. Monzurul Islam, et al. Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors. Int. J. Clin. Oncol. Cancer Res. 2018, 3(4), 49-54. doi: 10.11648/j.ijcocr.20180304.11

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AMA Style

Mohammad Sahajadul Alam, M. Mizanur Rahman, Md. Monowar Hossain, Mohammed Abu Kawsar Sarker, Md. Monzurul Islam, et al. Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors. Int J Clin Oncol Cancer Res. 2018;3(4):49-54. doi: 10.11648/j.ijcocr.20180304.11

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@article{10.11648/j.ijcocr.20180304.11,
  author = {Mohammad Sahajadul Alam and M. Mizanur Rahman and Md. Monowar Hossain and Mohammed Abu Kawsar Sarker and Md. Monzurul Islam and Rokeya Begum and Most Umme Habiba and Gazi Nurun Nahar Sultana},
  title = {Biography: Mutated hMLH1 Gene in Bangladeshi Gastric Cancer Patients: Its Relevance to Clinicopathological Factors},
  journal = {International Journal of Clinical Oncology and Cancer Research},
  volume = {3},
  number = {4},
  pages = {49-54},
  doi = {10.11648/j.ijcocr.20180304.11},
  url = {https://doi.org/10.11648/j.ijcocr.20180304.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcocr.20180304.11},
  abstract = {Like all cancers, Gastric cancer (GC) is also known to be of genetic origin. Genes are mutated in every steps of cell division, among them hMLH1 is a DNA mismatch gene which plays important role during carcinogenesis. This study was chalked out to find out the status of mutated hMLH1 Gene and its clinicopathological relevance among Bangladeshi gastric cancer patients. It was a cross sectional study conducted during January 2015 to April 2016. Tissue extracted from gastrectomy specimen of carcinoma stomach patients sent to specified laboratory. In the laboratory after DNA extraction, PCR, sequencing and analysis was done. In this study out of 19, exons 7 and 8 and introns 8 and 9 were studied after primer designing for hMLH1 gene mutation. After analysis, mutated gene were matched with clinicopathological parameters like age, sex, location of tumour, types and grade of tumour to see their relevance. Out of 45 patients, mutation was found in 15 patients (33.3%). Most gene alteration was found in elderly patients, more in male. Antral growth had more (80.0%) mutation and in ulceroproliferative type (66.7%) (p < .005). Mutation was common in intestinal type (73.3%) of GC. hMLH1 gene mutation found more in the moderately differentiated carcinoma patients. Factors like age, sex, morphology, Lauren’s type, grading, personal habits etc. might play pivotal role in the development of gene mutation. Multi-centre large study are required to extract more relevant information in this regard.},
 year = {2018}
}

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