Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice

Danny Ka-Ho Wong; Saisai Zhang; Gerald Wong; Ching Yan Serene Cheng; Regina Cheuk-Lam Lo; Fen Liu; Lung-Yi Mak; Peng Xiao; Wai-Kay Seto; Man Fung Yuen

doi:doi:10.11648/j.ijcm.20210502.11

Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice

Danny Ka-Ho Wong, Saisai Zhang, Gerald Wong, Ching Yan Serene Cheng, Regina Cheuk-Lam Lo, Fen Liu, Lung-Yi Mak, Peng Xiao, Wai-Kay Seto, Man Fung Yuen

Published in International Journal of Chinese Medicine (Volume 5, Issue 2)

Received: 29 March 2021 Accepted: 14 April 2021 Published: 26 April 2021

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Abstract

Background: Effective treatment against non-alcoholic fatty liver disease (NAFLD) is lacking. Song Zhi Wan (SZW), a Chinese formulation medicine comprising eight herbal ingredients, has been demonstrated to confer a liver protective effect in chronic hepatitis C patients. We aimed to investigate the effect of SZW on NAFLD using a high-fat diet (HFD; 60% fat) induced obese mouse model. Methods: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Serum total cholesterol and triglyceride levels, liver transaminases, and histologic steatosis were assessed. Results: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. HFD-fed mice receiving either SZW formulation had a smaller increase in serum total cholesterol (mean increase 7.9% – 39.4%) and a significant reduction in triglyceride (mean reduction 4.2% – 27.4%; P < 0.05). A dosage dependent effect on serum total cholesterol and triglyceride was observed with modified SZW (=0.043 and 0.006, respectively). 90% of placebo mice and 59% of SZW-treated mice had severe steatosis (P=0.079). With an escalating dosage of original SZW, there was a decreasing proportion of mice with severe steatosis (P=0.013). Conclusion: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice.

Published in	International Journal of Chinese Medicine (Volume 5, Issue 2)
DOI	10.11648/j.ijcm.20210502.11
Page(s)	22-28
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Dyslipidemia, Herbal Medicine, Liver Steatosis, Non-alcoholic Fatty Liver Disease, Obese Mice

References

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APA Style

Danny Ka-Ho Wong, Saisai Zhang, Gerald Wong, Ching Yan Serene Cheng, Regina Cheuk-Lam Lo, et al. (2021). Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice. International Journal of Chinese Medicine, 5(2), 22-28. https://doi.org/10.11648/j.ijcm.20210502.11

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ACS Style

Danny Ka-Ho Wong; Saisai Zhang; Gerald Wong; Ching Yan Serene Cheng; Regina Cheuk-Lam Lo, et al. Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice. Int. J. Chin. Med. 2021, 5(2), 22-28. doi: 10.11648/j.ijcm.20210502.11

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AMA Style

Danny Ka-Ho Wong, Saisai Zhang, Gerald Wong, Ching Yan Serene Cheng, Regina Cheuk-Lam Lo, et al. Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice. Int J Chin Med. 2021;5(2):22-28. doi: 10.11648/j.ijcm.20210502.11

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@article{10.11648/j.ijcm.20210502.11,
  author = {Danny Ka-Ho Wong and Saisai Zhang and Gerald Wong and Ching Yan Serene Cheng and Regina Cheuk-Lam Lo and Fen Liu and Lung-Yi Mak and Peng Xiao and Wai-Kay Seto and Man Fung Yuen},
  title = {Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice},
  journal = {International Journal of Chinese Medicine},
  volume = {5},
  number = {2},
  pages = {22-28},
  doi = {10.11648/j.ijcm.20210502.11},
  url = {https://doi.org/10.11648/j.ijcm.20210502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20210502.11},
  abstract = {Background: Effective treatment against non-alcoholic fatty liver disease (NAFLD) is lacking. Song Zhi Wan (SZW), a Chinese formulation medicine comprising eight herbal ingredients, has been demonstrated to confer a liver protective effect in chronic hepatitis C patients. We aimed to investigate the effect of SZW on NAFLD using a high-fat diet (HFD; 60% fat) induced obese mouse model. Methods: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Serum total cholesterol and triglyceride levels, liver transaminases, and histologic steatosis were assessed. Results: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. HFD-fed mice receiving either SZW formulation had a smaller increase in serum total cholesterol (mean increase 7.9% – 39.4%) and a significant reduction in triglyceride (mean reduction 4.2% – 27.4%; P P=0.079). With an escalating dosage of original SZW, there was a decreasing proportion of mice with severe steatosis (P=0.013). Conclusion: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice.},
 year = {2021}
}

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TY  - JOUR
T1  - Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice
AU  - Danny Ka-Ho Wong
AU  - Saisai Zhang
AU  - Gerald Wong
AU  - Ching Yan Serene Cheng
AU  - Regina Cheuk-Lam Lo
AU  - Fen Liu
AU  - Lung-Yi Mak
AU  - Peng Xiao
AU  - Wai-Kay Seto
AU  - Man Fung Yuen
Y1  - 2021/04/26
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcm.20210502.11
DO  - 10.11648/j.ijcm.20210502.11
T2  - International Journal of Chinese Medicine
JF  - International Journal of Chinese Medicine
JO  - International Journal of Chinese Medicine
SP  - 22
EP  - 28
PB  - Science Publishing Group
SN  - 2578-9473
UR  - https://doi.org/10.11648/j.ijcm.20210502.11
AB  - Background: Effective treatment against non-alcoholic fatty liver disease (NAFLD) is lacking. Song Zhi Wan (SZW), a Chinese formulation medicine comprising eight herbal ingredients, has been demonstrated to confer a liver protective effect in chronic hepatitis C patients. We aimed to investigate the effect of SZW on NAFLD using a high-fat diet (HFD; 60% fat) induced obese mouse model. Methods: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Serum total cholesterol and triglyceride levels, liver transaminases, and histologic steatosis were assessed. Results: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. HFD-fed mice receiving either SZW formulation had a smaller increase in serum total cholesterol (mean increase 7.9% – 39.4%) and a significant reduction in triglyceride (mean reduction 4.2% – 27.4%; P P=0.079). With an escalating dosage of original SZW, there was a decreasing proportion of mice with severe steatosis (P=0.013). Conclusion: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice.
VL  - 5
IS  - 2
ER  -

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Author Information

Danny Ka-Ho Wong

Department of Medicine, The University of Hong Kong, Hong Kong SAR
Saisai Zhang

Department of Medicine, The University of Hong Kong, Hong Kong SAR
Gerald Wong

Department of Medicine, The University of Hong Kong, Hong Kong SAR
Ching Yan Serene Cheng

Department of Medicine, The University of Hong Kong, Hong Kong SAR
Regina Cheuk-Lam Lo

State Key Laboratory of Liver Research, The University of Hong Kong, Hong Kong SAR
Fen Liu

Department of Gastroenterology and Hepatology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China
Lung-Yi Mak

Department of Medicine, The University of Hong Kong, Hong Kong SAR
Peng Xiao

Department of Medicine, The University of Hong Kong-Shenzhen Hospital, Shenzhen, China
Wai-Kay Seto

Department of Medicine, The University of Hong Kong, Hong Kong SAR
Man Fung Yuen

Department of Medicine, The University of Hong Kong, Hong Kong SAR

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Plain Text BibTeX RIS

APA Style

Danny Ka-Ho Wong, Saisai Zhang, Gerald Wong, Ching Yan Serene Cheng, Regina Cheuk-Lam Lo, et al. (2021). Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice. International Journal of Chinese Medicine, 5(2), 22-28. https://doi.org/10.11648/j.ijcm.20210502.11

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ACS Style

Danny Ka-Ho Wong; Saisai Zhang; Gerald Wong; Ching Yan Serene Cheng; Regina Cheuk-Lam Lo, et al. Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice. Int. J. Chin. Med. 2021, 5(2), 22-28. doi: 10.11648/j.ijcm.20210502.11

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AMA Style

Danny Ka-Ho Wong, Saisai Zhang, Gerald Wong, Ching Yan Serene Cheng, Regina Cheuk-Lam Lo, et al. Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice. Int J Chin Med. 2021;5(2):22-28. doi: 10.11648/j.ijcm.20210502.11

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@article{10.11648/j.ijcm.20210502.11,
  author = {Danny Ka-Ho Wong and Saisai Zhang and Gerald Wong and Ching Yan Serene Cheng and Regina Cheuk-Lam Lo and Fen Liu and Lung-Yi Mak and Peng Xiao and Wai-Kay Seto and Man Fung Yuen},
  title = {Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice},
  journal = {International Journal of Chinese Medicine},
  volume = {5},
  number = {2},
  pages = {22-28},
  doi = {10.11648/j.ijcm.20210502.11},
  url = {https://doi.org/10.11648/j.ijcm.20210502.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20210502.11},
  abstract = {Background: Effective treatment against non-alcoholic fatty liver disease (NAFLD) is lacking. Song Zhi Wan (SZW), a Chinese formulation medicine comprising eight herbal ingredients, has been demonstrated to confer a liver protective effect in chronic hepatitis C patients. We aimed to investigate the effect of SZW on NAFLD using a high-fat diet (HFD; 60% fat) induced obese mouse model. Methods: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Serum total cholesterol and triglyceride levels, liver transaminases, and histologic steatosis were assessed. Results: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. HFD-fed mice receiving either SZW formulation had a smaller increase in serum total cholesterol (mean increase 7.9% – 39.4%) and a significant reduction in triglyceride (mean reduction 4.2% – 27.4%; P P=0.079). With an escalating dosage of original SZW, there was a decreasing proportion of mice with severe steatosis (P=0.013). Conclusion: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice.},
 year = {2021}
}

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TY  - JOUR
T1  - Effect of a Herbal Formula Song Zhi Wan on Non-alcoholic Fatty liver Disease in Obese Mice
AU  - Danny Ka-Ho Wong
AU  - Saisai Zhang
AU  - Gerald Wong
AU  - Ching Yan Serene Cheng
AU  - Regina Cheuk-Lam Lo
AU  - Fen Liu
AU  - Lung-Yi Mak
AU  - Peng Xiao
AU  - Wai-Kay Seto
AU  - Man Fung Yuen
Y1  - 2021/04/26
PY  - 2021
N1  - https://doi.org/10.11648/j.ijcm.20210502.11
DO  - 10.11648/j.ijcm.20210502.11
T2  - International Journal of Chinese Medicine
JF  - International Journal of Chinese Medicine
JO  - International Journal of Chinese Medicine
SP  - 22
EP  - 28
PB  - Science Publishing Group
SN  - 2578-9473
UR  - https://doi.org/10.11648/j.ijcm.20210502.11
AB  - Background: Effective treatment against non-alcoholic fatty liver disease (NAFLD) is lacking. Song Zhi Wan (SZW), a Chinese formulation medicine comprising eight herbal ingredients, has been demonstrated to confer a liver protective effect in chronic hepatitis C patients. We aimed to investigate the effect of SZW on NAFLD using a high-fat diet (HFD; 60% fat) induced obese mouse model. Methods: C57BL/6J mice were fed with HFD for 10 weeks, followed by daily oral administration of various dosages of SZW (low [n=6], normal [n=10], high [n=10]) or water (n=10) for 8 weeks. Another formulation of SZW (modified SZW), in which two ingredients were replaced by radish seed and barley, was tested. Serum total cholesterol and triglyceride levels, liver transaminases, and histologic steatosis were assessed. Results: At the end of experiment, the HFD-fed placebo mice had a mean increase in serum total cholesterol and triglyceride by 57.7% and 35.0%, respectively. HFD-fed mice receiving either SZW formulation had a smaller increase in serum total cholesterol (mean increase 7.9% – 39.4%) and a significant reduction in triglyceride (mean reduction 4.2% – 27.4%; P P=0.079). With an escalating dosage of original SZW, there was a decreasing proportion of mice with severe steatosis (P=0.013). Conclusion: SZW administration can attenuate hypercholesterolemia, reduce dyslipidemia and alleviate steatosis in HFD-induced obese mice.
VL  - 5
IS  - 2
ER  -

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