FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of <i>Cissus Multistriata</i> and Physiological Changes Induced in Male Rats Exposed to <i>Naja Nigricollis</i> Venom

James Omale; Umar Farouq Idris; Ojodale Adejoh; Abu Paul

doi:doi:10.11648/j.ijcm.20170101.14

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FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom

James Omale, Umar Farouq Idris, Ojodale Adejoh, Abu Paul

Published in International Journal of Chinese Medicine (Volume 1, Issue 1)

Received: 3 February 2017 Accepted: 22 February 2017 Published: 9 March 2017

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Abstract

The present study sought to develop a blueprint for the identification of phyto-chemicals present in the methanol leaves extract of Cissus multistriata by Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometer (GC-MS) and test its antivenom activities against Naja nigricollis venom in male rats. Powdered leaves of C. multistriata were extracted using 50% methanol. FTIR analysis was carried out using SHIMADZU FTIR-8400S while GC-MS analysis was carried out using a SHIMADZU Japan GCMS-QP2010 PLUS mass spectrometer coupled to a SHIMADZU gas chromatograph. The antivenom property of the plant was tested by intraperitoneal (i. p.) injection of the extract at a dose of 100 mg/kg body weight of rats. Venom was administered i. p. at a dose of 1 mg/kg body weight of rats after 30 minutes. The IR spectrum suggested the presence of methyl, hydroxyl and unsaturated carbon-carbon double bonds in the bioactive compounds of the leaf extract. Two likely compounds as derived from the GC-MS chart are 2-(2-hydroxypropyl)-1,4-benzenediol, 2, 6, 8-Trimethylbicyclo[4, 2, 0]oct-2-ene-1,8-diol. Envenomation with N. nigricollis venom led to significant decreases in the levels of total protein and albumin with concomitant increase in creatinine, urea and uric acid levels. The plasma activities of alkaline phosphatase (ALP), aspartate amino transaminase (AST), and alkaline amino transferase (ALT) were significantly increased as well as the serum levels of sodium, potassium and chloride in rats challenged with venom compared with control rats. The above results indicate that C. multistriata contains numerous bioactive compounds and can neutralize N. nigricollis snake venom.

Published in	International Journal of Chinese Medicine (Volume 1, Issue 1)
DOI	10.11648/j.ijcm.20170101.14
Page(s)	24-31
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Bioactive Compounds, Electrolytes, Fourier Transform Infrared Spectroscopy, Gas Chromatography-Mass Spectrometer, Liver Function Tests, Plasma, Snake Venom

References

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James Omale, Umar Farouq Idris, Ojodale Adejoh, Abu Paul. (2017). FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom. International Journal of Chinese Medicine, 1(1), 24-31. https://doi.org/10.11648/j.ijcm.20170101.14

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James Omale; Umar Farouq Idris; Ojodale Adejoh; Abu Paul. FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom. Int. J. Chin. Med. 2017, 1(1), 24-31. doi: 10.11648/j.ijcm.20170101.14

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AMA Style

James Omale, Umar Farouq Idris, Ojodale Adejoh, Abu Paul. FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom. Int J Chin Med. 2017;1(1):24-31. doi: 10.11648/j.ijcm.20170101.14

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@article{10.11648/j.ijcm.20170101.14,
  author = {James Omale and Umar Farouq Idris and Ojodale Adejoh and Abu Paul},
  title = {FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom},
  journal = {International Journal of Chinese Medicine},
  volume = {1},
  number = {1},
  pages = {24-31},
  doi = {10.11648/j.ijcm.20170101.14},
  url = {https://doi.org/10.11648/j.ijcm.20170101.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20170101.14},
  abstract = {The present study sought to develop a blueprint for the identification of phyto-chemicals present in the methanol leaves extract of Cissus multistriata by Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometer (GC-MS) and test its antivenom activities against Naja nigricollis venom in male rats. Powdered leaves of C. multistriata were extracted using 50% methanol. FTIR analysis was carried out using SHIMADZU FTIR-8400S while GC-MS analysis was carried out using a SHIMADZU Japan GCMS-QP2010 PLUS mass spectrometer coupled to a SHIMADZU gas chromatograph. The antivenom property of the plant was tested by intraperitoneal (i. p.) injection of the extract at a dose of 100 mg/kg body weight of rats. Venom was administered i. p. at a dose of 1 mg/kg body weight of rats after 30 minutes. The IR spectrum suggested the presence of methyl, hydroxyl and unsaturated carbon-carbon double bonds in the bioactive compounds of the leaf extract. Two likely compounds as derived from the GC-MS chart are 2-(2-hydroxypropyl)-1,4-benzenediol, 2, 6, 8-Trimethylbicyclo[4, 2, 0]oct-2-ene-1,8-diol. Envenomation with N. nigricollis venom led to significant decreases in the levels of total protein and albumin with concomitant increase in creatinine, urea and uric acid levels. The plasma activities of alkaline phosphatase (ALP), aspartate amino transaminase (AST), and alkaline amino transferase (ALT) were significantly increased as well as the serum levels of sodium, potassium and chloride in rats challenged with venom compared with control rats. The above results indicate that C. multistriata contains numerous bioactive compounds and can neutralize N. nigricollis snake venom.},
 year = {2017}
}

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TY - JOUR
T1 - FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom
AU - James Omale
AU - Umar Farouq Idris
AU - Ojodale Adejoh
AU - Abu Paul
Y1 - 2017/03/09
PY - 2017
N1 - https://doi.org/10.11648/j.ijcm.20170101.14
DO - 10.11648/j.ijcm.20170101.14
T2 - International Journal of Chinese Medicine
JF - International Journal of Chinese Medicine
JO - International Journal of Chinese Medicine
SP - 24
EP - 31
PB - Science Publishing Group
SN - 2578-9473
UR - https://doi.org/10.11648/j.ijcm.20170101.14
AB - The present study sought to develop a blueprint for the identification of phyto-chemicals present in the methanol leaves extract of Cissus multistriata by Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometer (GC-MS) and test its antivenom activities against Naja nigricollis venom in male rats. Powdered leaves of C. multistriata were extracted using 50% methanol. FTIR analysis was carried out using SHIMADZU FTIR-8400S while GC-MS analysis was carried out using a SHIMADZU Japan GCMS-QP2010 PLUS mass spectrometer coupled to a SHIMADZU gas chromatograph. The antivenom property of the plant was tested by intraperitoneal (i. p.) injection of the extract at a dose of 100 mg/kg body weight of rats. Venom was administered i. p. at a dose of 1 mg/kg body weight of rats after 30 minutes. The IR spectrum suggested the presence of methyl, hydroxyl and unsaturated carbon-carbon double bonds in the bioactive compounds of the leaf extract. Two likely compounds as derived from the GC-MS chart are 2-(2-hydroxypropyl)-1,4-benzenediol, 2, 6, 8-Trimethylbicyclo[4, 2, 0]oct-2-ene-1,8-diol. Envenomation with N. nigricollis venom led to significant decreases in the levels of total protein and albumin with concomitant increase in creatinine, urea and uric acid levels. The plasma activities of alkaline phosphatase (ALP), aspartate amino transaminase (AST), and alkaline amino transferase (ALT) were significantly increased as well as the serum levels of sodium, potassium and chloride in rats challenged with venom compared with control rats. The above results indicate that C. multistriata contains numerous bioactive compounds and can neutralize N. nigricollis snake venom.
VL - 1
IS - 1
ER -

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Author Information

James Omale

Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Umar Farouq Idris

Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Ojodale Adejoh

Department of Biochemistry, Kogi State University, Anyigba, Nigeria
Abu Paul

Department of Biochemistry, Kogi State University, Anyigba, Nigeria

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James Omale, Umar Farouq Idris, Ojodale Adejoh, Abu Paul. (2017). FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom. International Journal of Chinese Medicine, 1(1), 24-31. https://doi.org/10.11648/j.ijcm.20170101.14

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James Omale; Umar Farouq Idris; Ojodale Adejoh; Abu Paul. FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom. Int. J. Chin. Med. 2017, 1(1), 24-31. doi: 10.11648/j.ijcm.20170101.14

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AMA Style

James Omale, Umar Farouq Idris, Ojodale Adejoh, Abu Paul. FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom. Int J Chin Med. 2017;1(1):24-31. doi: 10.11648/j.ijcm.20170101.14

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@article{10.11648/j.ijcm.20170101.14,
  author = {James Omale and Umar Farouq Idris and Ojodale Adejoh and Abu Paul},
  title = {FTIR and GC-MS Analyses of Phytochemicals from Methanol Leaf Extract of Cissus Multistriata and Physiological Changes Induced in Male Rats Exposed to Naja Nigricollis Venom},
  journal = {International Journal of Chinese Medicine},
  volume = {1},
  number = {1},
  pages = {24-31},
  doi = {10.11648/j.ijcm.20170101.14},
  url = {https://doi.org/10.11648/j.ijcm.20170101.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijcm.20170101.14},
  abstract = {The present study sought to develop a blueprint for the identification of phyto-chemicals present in the methanol leaves extract of Cissus multistriata by Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometer (GC-MS) and test its antivenom activities against Naja nigricollis venom in male rats. Powdered leaves of C. multistriata were extracted using 50% methanol. FTIR analysis was carried out using SHIMADZU FTIR-8400S while GC-MS analysis was carried out using a SHIMADZU Japan GCMS-QP2010 PLUS mass spectrometer coupled to a SHIMADZU gas chromatograph. The antivenom property of the plant was tested by intraperitoneal (i. p.) injection of the extract at a dose of 100 mg/kg body weight of rats. Venom was administered i. p. at a dose of 1 mg/kg body weight of rats after 30 minutes. The IR spectrum suggested the presence of methyl, hydroxyl and unsaturated carbon-carbon double bonds in the bioactive compounds of the leaf extract. Two likely compounds as derived from the GC-MS chart are 2-(2-hydroxypropyl)-1,4-benzenediol, 2, 6, 8-Trimethylbicyclo[4, 2, 0]oct-2-ene-1,8-diol. Envenomation with N. nigricollis venom led to significant decreases in the levels of total protein and albumin with concomitant increase in creatinine, urea and uric acid levels. The plasma activities of alkaline phosphatase (ALP), aspartate amino transaminase (AST), and alkaline amino transferase (ALT) were significantly increased as well as the serum levels of sodium, potassium and chloride in rats challenged with venom compared with control rats. The above results indicate that C. multistriata contains numerous bioactive compounds and can neutralize N. nigricollis snake venom.},
 year = {2017}
}

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