Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq

Ali Shakir Kamil Al-Suraifi; Ahmed Darwish Jabbar Al-Rubaie; Sareaa Maseer Gatya Al-Mayahie; Naeem Mohammed Mohsen Al-Abedy

doi:doi:10.11648/j.ijbecs.20160201.11

Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq

Ali Shakir Kamil Al-Suraifi, Ahmed Darwish Jabbar Al-Rubaie, Sareaa Maseer Gatya Al-Mayahie, Naeem Mohammed Mohsen Al-Abedy

Published in International Journal of Biomedical Engineering and Clinical Science (Volume 2, Issue 1)

Received: 29 August 2016 Accepted: 3 November 2016 Published: 9 December 2016

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Abstract

Hepatitis B virus (HBV) is the leading cause to liver disease, cirrhosis and primary liver cancer. About 1 million people die from HBV each year, which equates to about 2 HBV related deaths each minute. Depending on the virus sequence homogeneity as a minimum 10 genotypes (A to J) and numerous subgenotypes have been identified. Hepatitis B virus variants may be differ in their virulence, models of serologic reactivity, pathogenicity, response to treatment and global distribution. This study was carried out to detect HBV genotypes among Iraqi hepatitis type B patients in Wasit Province/ Iraq using nested PCR protocol. A total of 105 outpatients (65 males and 40 females, aged 1-95 years) clinically suspected as having viral hepatitis were included in this study. All the patients' sera (105 samples) were positive for HBV surface antigen (HBsAg) by ELISA screen test. Whereas 72 (60.5%) and 33 (31.4%) of these samples were positive and negative for HBV DNA, respectively, by first PCR. Survey of DNA positive samples for HBV genotypes by nested PCR (second PCR) demonstrated a unique results that no single genotype was found and all of these samples had mixed genotypes of which the pattern A+B+C+D+E was the most common (77.7%), followed by A+B+D+E (16.66%), A+B+C (2.77%), A+B+E (1.38%), and A+D+E (1.38%), whereas genotype F was not found in any patient. Statistically, there was non-significant difference in distribution of genotypes among males and females. The presence of mixed infection with about 5 HBV genotypes among most of our patients lead us to conclude that these patients are incurred to different sources of infection at different times and this required an epidemiological evaluation of HBV infection among our patients not only in Wasit Province but also all over Iraq to control this abnormal acquisition of these genotypes by Iraqi people.

Published in	International Journal of Biomedical Engineering and Clinical Science (Volume 2, Issue 1)
DOI	10.11648/j.ijbecs.20160201.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

HBV, Genotypes, Wasit Province, Iraq

References

[1]	Zainal IG, Safaa AA, Wajeeh KO. Biochemical parameters in relation to serum alphafetoprotein and leptin levels in Iraqi pateints with chronic liver diseases. Inter J life sci pharma res. 2013; 3(1): 16-22.
[2]	Brown RS, McMahon BJ, Lok ASF, Wong JB, Ahmed TA, Mouchli MA, Wang Z, Prokop LJ, Murad MH, Mohammed K. Antiviral therapy in chronic hepatitis B viral infection during pregnancy: a systematic review and meta-analysis. J Hepatology. 2016; 63(1): 319-333.
[3]	WHO Guidelines for the prevention, care and treatment of persons with chronic hepatitis B infection. Geneva, 2015; 27: 10-22.
[4]	Sunbul M. Hepatitis B virus genotypes: global distribution and clinical importance. World J Gastroenterology. 2014; 20(18): 5427-5434.
[5]	Valsamakis A. Molecular testing in the diagnosis and management of chronic hepatitis B. Clin Microbiol Rev. 2007; 20(3): 426-439.
[6]	Olinger CM, Lazouskaya NV, Eremin VF, Muller CP. Multiple genotypes and subtypes of hepatitis B and C viruses in Belarus: similarities with Russia and western European influences. Clin Microbiol Infect. 2008; 14(issue 6):575-581.
[7]	Allain JP. Epidemiology of hepatitis B virus and genotype. J Clin Virol. 2006; 36 (Suppl 1): S12-S17.
[8]	Mojiri A, Khadang B, Moaddeb A, Ghaedi M, Heidari T, Torab A, Salah A, Amirzadeh S, Jowkar Z, Mehrabani D, Amini-Bavil-Olyaee S, Behzad-Behbahani A, Dehyadegari M, Saberifirozi M, Ardabili M, Beheshti M, Rahsaz M, Banihashemi M, Azarpira N, Geramizadeh. B Hepatitis B virus genotypes in southwest Iran: molecular, serological and clinical outcomes. World J Gastroenterology. 2008; 14(10):1510-1513.
[9]	Liu CJ, Kao JH. Global perspective on the natural history of chronic hepatitis B: role of hepatitis B virus genotypes A. J. Semin Liver Dis. 2013; 33(2):97-102.
[10]	Mello FC, Melo MM, Martins RM, Gomes SA, Souto FJ, Nabuco LC, Villela-Nogueira CA, Coelho HS, Franz HC, Saraiva JC, Virgolino HA, Motta-Castro AR. Hepatitis B virus genotypes circulating in Brazil: molecular characterization of genotype F isolates. BMC Microbiol. 2007; 7(103): 1-9.
[11]	Naito H, Hayashi S, Abe K. Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers. J Clin Microbiol. 2001; 39(1): 362-364.
[12]	Baig S, Siddiqui AA, Ahmed W, Qureshi H, Arif A. The association of complex liver disorders with HBV genotypes prevalent in Pakistan. Virol J. 2007; 4(128): 1-7.
[13]	Ross SM. Introduction to probability and statistics for Engineers and Scientists (4th edn.), Elsevier, London. 2009.
[14]	Perrillo R, Richman D, Sherman K. Pocket guide to hepatitis B. University of Wisconsin Board of Regents and MDG Development Group. (2009): 1-63.
[15]	Raimondo G, Allain JP, Brunetto MR, Buendia MA, Chen DS, Colombo M, Craxì A, Donato F, Ferrari C, Gaeta GB, Gerlich WH, Levrero M, Locarnini S, Michalak T, Mondelli MU, Pawlotsky JM, Pollicino T, Prati D, Puoti M, Samuel D, Shouval D, Smedile A, Squadrito G, Trépo C, Villa E, Will H, Zanetti AR, Zoulim F. Statements from the Taormina expert meeting on occult hepatitis B virus infection. J Hepatology. 2008; 49(4): 652-657.
[16]	AL-Hmudi AH. Virological and immunogenetic study of hepatitis B virus in patients of Basra Province–Iraq. Ph.D. thesis in Microbiology, College of Science, University of Basrah. 2011.
[17]	Salim RW, Abdullah BA. The Prevalence of hepatitis B virus in high risk groups in Nineveh Governorate/ Iraq. J Baghdad Sci. 2014; 11(2): 888-893.
[18]	Al-Aboudi SAK, Al-Hmudi HA. Some of immunogenetic status of HBsAg negative, HBcAb positive Blood donors in Basra Province-Iraq. Ann Biol Res. 2015; 6(4): 47-54.
[19]	Khaled IAE, Mahmoud OM, Saleh AF, Baioumi EA. Prevalence of HBV genotypes in Egypt among hepatitis patients. J Ame Sci. 2010; 6(11): 185-190.
[20]	Ghafourian S, Mohebi R, Khosravi A, Maleki A, Davoodian A, Sadeghifard N. Detection of hepatitis B virus DNA by Real-Time PCR in chronic hepatitis B patients, Ilam, Iran. Middle-East J Sci Res. 2011; 9 (4): 478-480.
[21]	Weber B, Melchior W, Gehrke R, Doerr HW, Berger A, Rabenau H. Hepatitis B virus marker in anti HBc only positive individuals. J Med Virol. 2001; 64(3): 309-312.
[22]	Keeffe EB, Dieterich DT, Han SHB, Jacobson IM, Martin P, Schiff ER, Tobias H, Wright TL. A Treatment algorithm for the management of chronic hepatitis B virus infection in the United States: an update. J Clin Gastroenterolgy Hepatology. 2006; 4(8): 936-962.
[23]	Yim HJ, Lok AS. Natural history of chronic hepatitis B virus infection: what we knew in 1981 and what we know in 2005. J Hepatology. 2006; 43(2 Suppl 1): 173-181.
[24]	Fattovich G, Bortolotti F, Donato F. Natural history of chronic hepatitis B: special emphasis on disease progression and prognostic factors. J Hepatology. 2008; 48(2): 335-352.
[25]	McMahon BJ. The natural history of chronic hepatitis B virus infection. Hepatology. 2009; 49 (5): S45-S55.
[26]	Gish RG, Given BD, Lai CL, Locarnini SA, Lau JY, Lewis DL, Schluep T. Chronic hepatitis B: virology, natural history, current management and a glimpse at future opportunities. J antiviral res. 2015; 121: 47-58.
[27]	Bartholomeusz A, Schaefer S. Hepatitis B virus genotypes: comparison of genotyping methods. Rev Med Virol. 2004; 14: 3-16.
[28]	Toan NL, Song LH, Kremsner PG, Duy DN, Binh VQ, Koeberlein B, Kaiser S, Kandolf R, Torresi J, Bock CT. Impact of the hepatitis B virus genotype and genotype mixtures on the course of liver disease in Vietnam. J Hepatology. 2006; 43(6): 1375-1384.
[29]	Geramizadeh B, Kaboli R, Behzad-Behbahani A, Rahsaz M, Azarpira N, Aghdai M, Aytollahi M, Yaghoobi R, Baneehashemee M. A nested PCR method for the identification of hepatitis B virus genotype in paraffin blocks of formalin-fixed liver biopsies. Arch Iranian Med. 2008; 11(4): 455-458.
[30]	Rashid PMA, Salih GF. Identification and genotyping of hepatitis B virus by PCR assay using genotype specific primers. Europ Sci J. 2014; 10 (9): 424-433.
[31]	Ahmed AM. Determination of hepatitis B virus genotypes among Iraqi chronic hepatitis B patients and inactive HBV carriers. Ph.D. Thesis, Genetic Engineering and Biotechnology Institute, Baghdad University, Iraq, 2013; pp. 195.
[32]	Issa AH, Hamdan NA, Thwiny HT. Hepatitis B virus subclinical case infection in Basra patients discovered by preoperation of viral screening investigations. World J Pharm Res. 2015; 4(4): 1599-1610.
[33]	Abdo AA, Al-Jarallah BM, Sanai FM, Hersi AS, Al-Swat K, Azzam NA, Al-Dukhayil M, Al-Maarik A, Al-Faleh FZ. Hepatitis B genotypes: relation to clinical outcome in patients with chronic hepatitis B in Saudi Arabia. World J Gastroenterology. 2006; 12(43): 7019-7024.
[34]	Zekri AR, Hafez MM, Mohamed NI, Hassan ZK, El-Sayed MH, Khaled MM, Mansour T. Hepatitis B virus (HBV) genotypes in Egyptian pediatric cancer patients with acute and chronic active HBV infection. Virol J. 2007; 4(74): 1-7.
[35]	Yoosefi K, Taheri M, Khosravi S, Saneie-Moghadam E, Mohagheghi-Fard AH. Genotyping of hepatitis B virus by multiplex PCR in Sistan and Baluchestan Province. Zahedan. J Res Med Sci. 2015); 15: 29-33.
[36]	Chen B, Kao FJ, Liu CJ, Chen DS, Chen PJ. Genotypic dominance and novel recombinations in HBV genotype B and C coinfected intravenous drug users. J Med Virol. 2004; 73: 13-22.
[37]	Mercier M, Laperche S, Girault A, Sureau C, Servant-Delmas A. Overestimation of incidence of hepatitis B virus mixed-genotype infections by use of the new line probe INNO-LiPA genotyping assay. J Clin Microbiol. 2011; 49(3). 1154-1156.
[38]	Antaki N, Haffar S, Ali DS, Assaad F, Abou HR, Zeibane N, Nasserelddine M, Ibrahim N, Alhaj N, Jabbour E, Aaraj R, Antaki F, Kebbewar K. High prevalence of HBV genotype D in Syria and the clinical characteristics of hepatitis B e antigen-negative chronic hepatitis B. J Epidemiol Infect. 2010; 138(1): 40-44.
[39]	Bozdayi G, Türkyilmaz AR, Idilman R, Karatayli E, Rota S, Yurdaydin C, Bozdayi AM. Complete genome sequence and phylogenetic analysis of hepatitis B virus isolated from Turkish patients with chronic HBV infection. J Med Virol. 2005; 76(4): 476-481.
[40]	Yalcin K, Degertekin H, Bahcecioglu IH, Demir A, Aladag M, Yildirim B, Horasanli S, Ciftci S, Badur S. Hepatitis B virus genotype D prevails in patients with persistently elevated or normal ALT levels in Turkey. J Infect. 2004; 32(1). 24-29.
[41]	Nie JJ, Sun KX, Li J, Wang J, Jin H, Wang L, Lu FM, Li T, Yan L, Yang JX, Sun MS, Zhuang H. A type-specific nested PCR assay established and applied for investigation of HBV genotype and subgenotype in Chinese patients with chronic HBV infection. Virolo J. 2012; 9(121): 1-9.
[42]	Chen BF, Chen PJ, Jow GM, Sablon E, Liu CJ, Chen DS, Kao JH. High prevalence of mixed genotype infections in hepatitis B virus infected intravenous drug users. J Med Virol. 2004; 74(4): 536-42.

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APA Style

Ali Shakir Kamil Al-Suraifi, Ahmed Darwish Jabbar Al-Rubaie, Sareaa Maseer Gatya Al-Mayahie, Naeem Mohammed Mohsen Al-Abedy. (2016). Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq. International Journal of Biomedical Engineering and Clinical Science, 2(1), 1-7. https://doi.org/10.11648/j.ijbecs.20160201.11

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Ali Shakir Kamil Al-Suraifi; Ahmed Darwish Jabbar Al-Rubaie; Sareaa Maseer Gatya Al-Mayahie; Naeem Mohammed Mohsen Al-Abedy. Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq. Int. J. Biomed. Eng. Clin. Sci. 2016, 2(1), 1-7. doi: 10.11648/j.ijbecs.20160201.11

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Ali Shakir Kamil Al-Suraifi, Ahmed Darwish Jabbar Al-Rubaie, Sareaa Maseer Gatya Al-Mayahie, Naeem Mohammed Mohsen Al-Abedy. Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq. Int J Biomed Eng Clin Sci. 2016;2(1):1-7. doi: 10.11648/j.ijbecs.20160201.11

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@article{10.11648/j.ijbecs.20160201.11,
  author = {Ali Shakir Kamil Al-Suraifi and Ahmed Darwish Jabbar Al-Rubaie and Sareaa Maseer Gatya Al-Mayahie and Naeem Mohammed Mohsen Al-Abedy},
  title = {Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq},
  journal = {International Journal of Biomedical Engineering and Clinical Science},
  volume = {2},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ijbecs.20160201.11},
  url = {https://doi.org/10.11648/j.ijbecs.20160201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20160201.11},
  abstract = {Hepatitis B virus (HBV) is the leading cause to liver disease, cirrhosis and primary liver cancer. About 1 million people die from HBV each year, which equates to about 2 HBV related deaths each minute. Depending on the virus sequence homogeneity as a minimum 10 genotypes (A to J) and numerous subgenotypes have been identified. Hepatitis B virus variants may be differ in their virulence, models of serologic reactivity, pathogenicity, response to treatment and global distribution. This study was carried out to detect HBV genotypes among Iraqi hepatitis type B patients in Wasit Province/ Iraq using nested PCR protocol. A total of 105 outpatients (65 males and 40 females, aged 1-95 years) clinically suspected as having viral hepatitis were included in this study. All the patients' sera (105 samples) were positive for HBV surface antigen (HBsAg) by ELISA screen test. Whereas 72 (60.5%) and 33 (31.4%) of these samples were positive and negative for HBV DNA, respectively, by first PCR. Survey of DNA positive samples for HBV genotypes by nested PCR (second PCR) demonstrated a unique results that no single genotype was found and all of these samples had mixed genotypes of which the pattern A+B+C+D+E was the most common (77.7%), followed by A+B+D+E (16.66%), A+B+C (2.77%), A+B+E (1.38%), and A+D+E (1.38%), whereas genotype F was not found in any patient. Statistically, there was non-significant difference in distribution of genotypes among males and females. The presence of mixed infection with about 5 HBV genotypes among most of our patients lead us to conclude that these patients are incurred to different sources of infection at different times and this required an epidemiological evaluation of HBV infection among our patients not only in Wasit Province but also all over Iraq to control this abnormal acquisition of these genotypes by Iraqi people.},
 year = {2016}
}

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TY  - JOUR
T1  - Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq
AU  - Ali Shakir Kamil Al-Suraifi
AU  - Ahmed Darwish Jabbar Al-Rubaie
AU  - Sareaa Maseer Gatya Al-Mayahie
AU  - Naeem Mohammed Mohsen Al-Abedy
Y1  - 2016/12/09
PY  - 2016
N1  - https://doi.org/10.11648/j.ijbecs.20160201.11
DO  - 10.11648/j.ijbecs.20160201.11
T2  - International Journal of Biomedical Engineering and Clinical Science
JF  - International Journal of Biomedical Engineering and Clinical Science
JO  - International Journal of Biomedical Engineering and Clinical Science
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2472-1301
UR  - https://doi.org/10.11648/j.ijbecs.20160201.11
AB  - Hepatitis B virus (HBV) is the leading cause to liver disease, cirrhosis and primary liver cancer. About 1 million people die from HBV each year, which equates to about 2 HBV related deaths each minute. Depending on the virus sequence homogeneity as a minimum 10 genotypes (A to J) and numerous subgenotypes have been identified. Hepatitis B virus variants may be differ in their virulence, models of serologic reactivity, pathogenicity, response to treatment and global distribution. This study was carried out to detect HBV genotypes among Iraqi hepatitis type B patients in Wasit Province/ Iraq using nested PCR protocol. A total of 105 outpatients (65 males and 40 females, aged 1-95 years) clinically suspected as having viral hepatitis were included in this study. All the patients' sera (105 samples) were positive for HBV surface antigen (HBsAg) by ELISA screen test. Whereas 72 (60.5%) and 33 (31.4%) of these samples were positive and negative for HBV DNA, respectively, by first PCR. Survey of DNA positive samples for HBV genotypes by nested PCR (second PCR) demonstrated a unique results that no single genotype was found and all of these samples had mixed genotypes of which the pattern A+B+C+D+E was the most common (77.7%), followed by A+B+D+E (16.66%), A+B+C (2.77%), A+B+E (1.38%), and A+D+E (1.38%), whereas genotype F was not found in any patient. Statistically, there was non-significant difference in distribution of genotypes among males and females. The presence of mixed infection with about 5 HBV genotypes among most of our patients lead us to conclude that these patients are incurred to different sources of infection at different times and this required an epidemiological evaluation of HBV infection among our patients not only in Wasit Province but also all over Iraq to control this abnormal acquisition of these genotypes by Iraqi people.
VL  - 2
IS  - 1
ER  -

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Author Information

Ali Shakir Kamil Al-Suraifi

Department of Biology, College of Science, Wasit University, Al-Kut, Iraq
Ahmed Darwish Jabbar Al-Rubaie

Department of Biology, College of Science, Wasit University, Al-Kut, Iraq
Sareaa Maseer Gatya Al-Mayahie

Department of Biology, College of Science, Wasit University, Al-Kut, Iraq
Naeem Mohammed Mohsen Al-Abedy

Molecular Biology Laboratory, Alkarama Teaching Hospital, Al-Kut, Iraq

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APA Style

Ali Shakir Kamil Al-Suraifi, Ahmed Darwish Jabbar Al-Rubaie, Sareaa Maseer Gatya Al-Mayahie, Naeem Mohammed Mohsen Al-Abedy. (2016). Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq. International Journal of Biomedical Engineering and Clinical Science, 2(1), 1-7. https://doi.org/10.11648/j.ijbecs.20160201.11

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ACS Style

Ali Shakir Kamil Al-Suraifi; Ahmed Darwish Jabbar Al-Rubaie; Sareaa Maseer Gatya Al-Mayahie; Naeem Mohammed Mohsen Al-Abedy. Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq. Int. J. Biomed. Eng. Clin. Sci. 2016, 2(1), 1-7. doi: 10.11648/j.ijbecs.20160201.11

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AMA Style

Ali Shakir Kamil Al-Suraifi, Ahmed Darwish Jabbar Al-Rubaie, Sareaa Maseer Gatya Al-Mayahie, Naeem Mohammed Mohsen Al-Abedy. Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq. Int J Biomed Eng Clin Sci. 2016;2(1):1-7. doi: 10.11648/j.ijbecs.20160201.11

Copy | Download

@article{10.11648/j.ijbecs.20160201.11,
  author = {Ali Shakir Kamil Al-Suraifi and Ahmed Darwish Jabbar Al-Rubaie and Sareaa Maseer Gatya Al-Mayahie and Naeem Mohammed Mohsen Al-Abedy},
  title = {Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq},
  journal = {International Journal of Biomedical Engineering and Clinical Science},
  volume = {2},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.ijbecs.20160201.11},
  url = {https://doi.org/10.11648/j.ijbecs.20160201.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijbecs.20160201.11},
  abstract = {Hepatitis B virus (HBV) is the leading cause to liver disease, cirrhosis and primary liver cancer. About 1 million people die from HBV each year, which equates to about 2 HBV related deaths each minute. Depending on the virus sequence homogeneity as a minimum 10 genotypes (A to J) and numerous subgenotypes have been identified. Hepatitis B virus variants may be differ in their virulence, models of serologic reactivity, pathogenicity, response to treatment and global distribution. This study was carried out to detect HBV genotypes among Iraqi hepatitis type B patients in Wasit Province/ Iraq using nested PCR protocol. A total of 105 outpatients (65 males and 40 females, aged 1-95 years) clinically suspected as having viral hepatitis were included in this study. All the patients' sera (105 samples) were positive for HBV surface antigen (HBsAg) by ELISA screen test. Whereas 72 (60.5%) and 33 (31.4%) of these samples were positive and negative for HBV DNA, respectively, by first PCR. Survey of DNA positive samples for HBV genotypes by nested PCR (second PCR) demonstrated a unique results that no single genotype was found and all of these samples had mixed genotypes of which the pattern A+B+C+D+E was the most common (77.7%), followed by A+B+D+E (16.66%), A+B+C (2.77%), A+B+E (1.38%), and A+D+E (1.38%), whereas genotype F was not found in any patient. Statistically, there was non-significant difference in distribution of genotypes among males and females. The presence of mixed infection with about 5 HBV genotypes among most of our patients lead us to conclude that these patients are incurred to different sources of infection at different times and this required an epidemiological evaluation of HBV infection among our patients not only in Wasit Province but also all over Iraq to control this abnormal acquisition of these genotypes by Iraqi people.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Unusual HBV Mixed Genotype Infections Among Hepatitis Type B Iraqi Patients in Wasit Province/Iraq
AU  - Ali Shakir Kamil Al-Suraifi
AU  - Ahmed Darwish Jabbar Al-Rubaie
AU  - Sareaa Maseer Gatya Al-Mayahie
AU  - Naeem Mohammed Mohsen Al-Abedy
Y1  - 2016/12/09
PY  - 2016
N1  - https://doi.org/10.11648/j.ijbecs.20160201.11
DO  - 10.11648/j.ijbecs.20160201.11
T2  - International Journal of Biomedical Engineering and Clinical Science
JF  - International Journal of Biomedical Engineering and Clinical Science
JO  - International Journal of Biomedical Engineering and Clinical Science
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2472-1301
UR  - https://doi.org/10.11648/j.ijbecs.20160201.11
AB  - Hepatitis B virus (HBV) is the leading cause to liver disease, cirrhosis and primary liver cancer. About 1 million people die from HBV each year, which equates to about 2 HBV related deaths each minute. Depending on the virus sequence homogeneity as a minimum 10 genotypes (A to J) and numerous subgenotypes have been identified. Hepatitis B virus variants may be differ in their virulence, models of serologic reactivity, pathogenicity, response to treatment and global distribution. This study was carried out to detect HBV genotypes among Iraqi hepatitis type B patients in Wasit Province/ Iraq using nested PCR protocol. A total of 105 outpatients (65 males and 40 females, aged 1-95 years) clinically suspected as having viral hepatitis were included in this study. All the patients' sera (105 samples) were positive for HBV surface antigen (HBsAg) by ELISA screen test. Whereas 72 (60.5%) and 33 (31.4%) of these samples were positive and negative for HBV DNA, respectively, by first PCR. Survey of DNA positive samples for HBV genotypes by nested PCR (second PCR) demonstrated a unique results that no single genotype was found and all of these samples had mixed genotypes of which the pattern A+B+C+D+E was the most common (77.7%), followed by A+B+D+E (16.66%), A+B+C (2.77%), A+B+E (1.38%), and A+D+E (1.38%), whereas genotype F was not found in any patient. Statistically, there was non-significant difference in distribution of genotypes among males and females. The presence of mixed infection with about 5 HBV genotypes among most of our patients lead us to conclude that these patients are incurred to different sources of infection at different times and this required an epidemiological evaluation of HBV infection among our patients not only in Wasit Province but also all over Iraq to control this abnormal acquisition of these genotypes by Iraqi people.
VL  - 2
IS  - 1
ER  -

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