| Peer-Reviewed

Trace Elements Composition and Enzymes Activity of Soil Amended with Municipal Sewage Sludge at Three Locations in Kentucky

Received: 13 April 2020     Accepted: 30 April 2020     Published: 23 September 2020
Views:       Downloads:
Abstract

Biological monitoring is a sensitive indicator of soil ecological stress for early restoration. The addition of manure, such as sewage sludge (SS) to agricultural soil contributes to the enhancement of organic compounds. However, SS may contain toxic metals that potentially affect soil microbial growth and the enzymes they produce. Soil samples were collected from three agricultural locations in Kentucky (Adair, Meade, and Franklin Counties), from areas where municipal SS was applied as a soil amendment for commercial crop production. The objectives of this investigation were to: 1) assess the impact of mixing native agricultural soil with municipal SS on the activities of the three enzymes that hydrolyze urea (urease, urea amidohydrolase, EC 3.5.1.5), sucrose (invertase, β-D-fructofuranosidase), and p-nitrophenyl phosphate (acid and alkaline phosphatase) and 2) determine total microbial activity using the fluorescein diacetate reagent. The concentrations of Cr, Ni, Cu, Zn, Pb, and C content in soil mixed with SS in Franklin location were greater compared to Adair and Meade locations. The elevated soil urease and invertase activities (47 and 89%, respectively) as well as acid and alkaline phosphatase activities (23 and 26%, respectively) in soil amended with SS provided evidence of increased soil microbial population and the enzymes they produce.

Published in International Journal of Applied Agricultural Sciences (Volume 6, Issue 5)
DOI 10.11648/j.ijaas.20200605.11
Page(s) 89-95
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Biosolids, Urease. Invertase, Phosphatase, Total Enzymes Activity, Soil Management Practice

References
[1] Angelovicova L, M Lodenius, E Tulisalo, D Fazekasova. Effect of heavy metals on soil enzyme activity at different field conditions. In Middle Spis mining area (Slovakia). Bull Environ Contam Toxicol 2014, 93: 670-675.
[2] Hargreaves JC, MS Adl, PR Warman. A review of the use of composted municipal solid waste in agriculture. Agr. Ecosyst. Environ. 2008, 123: 1–14.
[3] Antonious, GF. Impact of soil management practices on yield, fruit quality, and antioxidant contents of pepper at four stages of fruit development. J. Environmental Sci. Health, 2014, Part B49: 769-774.
[4] Antonious GF, ET Turley, RR Hill, JC Snyder. Effect of municipal refuse and chicken manure applications on kale and collard green yields and quality, Fruit and Vegetable Research Report, December 30, 2013, PR-673, pages 37-39, University of Kentucky, College of Agriculture, Food and Environment, Lexington, KY 40546.
[5] Antonious G. F. (2003). Impact of soil management and two botanical insecticides on urease and invertase activity. J. Environ. Sci. Health 38: 479-488.
[6] Ozores-Hampton M, R Peach. Biosolids in vegetable production systems. Hort. Technology 2002, 12 (3), 336-340.
[7] Antonious GF. Enzyme activities and heavy metals concentration in soil amended with sewage sludge. J. Environ. Science & Health, Part-A Toxic/Hazardous Substances & Environmental Engineering, 2009, A44, Issue No. 10, p 1019-1024.
[8] Terry RE. Use of soil conditioners to enhance and speed bioremediation of contaminated soils. In: Handbook of Soil Conditioners. Wallace, A., Terry, R. E., Eds., Marcel Dekker Inc., New York, 1998, 551-751.
[9] Antonious GF, JC Snyder. Accumulation of heavy metals in plants and potential phytoremediation of lead by potato, Solanum tuberosum L. J. Environ. Sci. Health 2007, A42, 811-816.
[10] Shiralipour A, DB McConnell, WH Smith. Physical and chemical properties of soils as affected by municipal solid waste compost application. Biomass and Bioenergy 1992, 3, 261-266.
[11] Plaza C, A Polo, G Brunetti, J Garcia-Gil, V D’Orazio. Soil fulvic acid properties as a means to assess the use of pig amendment, Soil Till. Res. 2003, 74: 179–190.
[12] Graber E., I Dror, F Bercovich, M Rosner. Enhanced transport of pesticides in a field trial with treated sewage sludge, Chemosphere 2001, 44: 805–811.
[13] Marschner P, E Kandeler, B Marschner. Structure and function of the soil microbial community in a long-term fertilizer experiment, Soil Biol. Biochem. 2003, 35: 453–461.
[14] Namkoong W, E Hwang, J Park, J Choi. J. Bioremediation of diesel contaminated soil with composting, Environ. Pollut. 2002, 119: 23–31.
[15] Zagal E, N Rodr´ıguez, I Vidal, L Quezada. Actividad microbiana en un suelo de origen volc´anico bajo distinto manejo agron´omico, Agri. T´ec. 2002, 62: 297–309.
[16] Dungan, R., A Ibekwe, S Yates. Effects of propargyl bromide and 1.3-dichloropropene on microbial communities in an organycally amended soil, FEMS Microbiol. Ecol. 2003, 43: 75–87.
[17] B¨ohme, L., U Langer, F B¨ohme. Microbial biomass, enzyme activities and microbial community structure in two European long-term field experiments, Agric. Ecosyst. Environ. 2005, 109: 141–152.
[18] Wanner, U., F F¨uhr, P Burauel. Influence of the amendment of corn straw on the degradation behaviour of the fungicide dithianon in soil, Environ. Pollution 2005, 133: 63–70.
[19] Saha SK, E Pfeufer, R Bessin, S Wright, J Strang. Vegetable Production Guide for Commercial Growers, Cooperative Extension Service Bulletin ID-36, University of Kentucky, College of Agriculture, 2016, Lexington, KY, ID-36.
[20] McLaren AD, AH Pukite, I Barshad. Isolation of humus with enzymatic activity from soil. Soil Sci. 1975, 119: 178-180.
[21] American Public Health Association (APHA). Standard methods for the determination of water and wastewater, 19th ed. 1995.
[22] EPA. Method 6020a: Inductively coupled plasma -mass spectrometry. USEPA: Washington, DC, 1998.
[23] Tabatabi MA, JM Bremner. Assay of urease activity in soils. Soil Biol. Biochem. 1972, 4: 479-487.
[24] Balasubramanian, D, DJ Bagyaraj, G Rangaswami. Studies on the influence of foliar application of chemicals on the microflora and certain enzyme activities in the rhizosphere of Eleusine coracana Gaertn. Plant Soil 1970, 32: 198-206.
[25] Tabatabai MA, JM Bremner. Use of p-nitrophenol phosphate for assay of soil phosphatase activity. Soil Biol. Biochem. 1969, 1: 301-307.
[26] Chakrabarti K, P Bhattacharya, A Chakraborty. Effects of metal-contaminated organic wastes on microbial biomass and activities: A review. In: Heavy Metal Contamination of Soil, Ahmed I., Hayat S, and Pichtel J. (eds), Scince Publishers, Inc., Plymouth, UK, 2005, p 195-204.
[27] Antonious GF. Enzymatic activity and half-lives of capsaicin, dihydrocapsaicin, and pentadecanoic acid methyl ester in soil sprayed with hot pepper extract. J. Environmental Science and Health, 2013. Part-B, 48: 836-843.
[28] SAS Institute Inc. SAS/STAT Guide, Version 6.4 SAS Inc., Campus Drive, Cary, NC 27513; 2016.
[29] Garcia C, T Hernandez, C Costa, B Ceccanti, G Masciandaro, C Ciardi. A study of biochemical parameters of composted and fresh municipal wastes. Bioresource Technology 1993, 44: 17-23.
[30] Yang, Z., Liu, S., Zheng, D., Feng, S. (2006). Effects of cadmium, zinc and lead on soil enzyme activities. J. Environmental Sciences 18, 6, 1135-1141.
[31] Kucharski J, E Jastrzebska, J Wyszkowska, A Hlasko. Effect of pollution with diesel oil and leaded petrol on enzymatic activity of the soil. Zesz. Probl. Postpep. Nauk Rol. 2000, 472: 457-464.
[32] Moore PA, TC Daniel, JT Gilmour, BR Shreve, DR Edwards, BH Wood. Decreasing metal runoff from poultry litter with aluminum sulfate. J Environ Quality 1998, 27: 92-99.
[33] Acosta-Martinez V, Z Reicher, M Bischoff, RF Turco. The role of tree leaf mulch and nitrogen fertilizer on turfgrass soil quality. Biol. Fertil. Soils 1999, 29: 55-61.
[34] Straton ML, JE Rechcigl. Organic mulches, wood products, and compost as soil amendments and conditioners. In: Handbook of soil conditioners. Wallace A, Terry R (eds), Marcel Dekker, Inc., 1998, New York, pp. 43-95.
[35] Lalande R, V Furlan, DA Angers, G Lemieux G. Soil improvement following addition of chipped wood from twigs. Amer. J. Altern. Agric. 1998, 13: 132-137.
[36] Gunasekara AS, B Xing. Sorption and desorption of naphthalate by soil organic matter. J. Environ. Qual. 2003, 32: 240-246.
[37] Antonious GF, GA Patel, JC Snyder, MS Coyne. Pyrethrins and piperonyl butoxide adsorption to soil organic matter. J. Environ. Sci. Health 2004, B39: 19-32.
[38] Antonious GF, JC Snyder. Impact of soil incorporated sewage sludge on herbicide and trace metal mobility in the environment, In: Environmental Engineering & Economics, published 2006 by Dr. Michael Theophanides, Institute for Education & Research, Athens, Greece, Chapter 12, pages 149-164.
[39] Datta A, SK Sanyal, S Saha. A study on natural and synthetic humic acids and their complexing ability towards cadmium. Plant Soil 2001, 235: 115-125.
[40] Tejada M, MT Hernandez, C Garcia. Application of two organic wastes in a soil polluted by lead: Effects on the soil enzymatic activities. J. Environ. Qual. 2007, 36: 216-225.
[41] Jensen, LS and Sommer, SG (2013). Manure Organic Matter – Characteristics and Microbial Transformations. Chapter 5, pages 67-87. In: Animal Manure Recycling Treatment and Management Edited by Sommer, SG.; Christensen, ML; Schmidt, T; and Jensen, LS. John Wiley & Sons Ltd, The Atrium, Southern Gate, Chichester, West Sussex, PO19 8SQ, United Kingdom.
[42] Antonious GF, Z Ray, L Rivers. Sewage sludge reduced dimethoate residues in runoff water. UK Agricultural Experimental Station, Fruit & Vegetable Crops Research Report 2007, # PR-555, pp. 85-87, University of Kentucky, Lexington, KY 40546.
[43] Antonious GF Mobility and half-life of bensulide in agricultural soil. J. Environ. Science Health, Part-B Pesticides, Food Contaminants & Agricultural Wastes, 2010, B45: 1-10.
[44] Eid, E. M., Hussain, A. A. Taher, M. A., Galal, T. M., Shaltout, K. H., Sewelam, N. (2020). Sewage sludge application enhances the growth of Corchorus olitorius plants and provides a sustainable practice for nutrient recirculation in agricultural soils. J. Soil Sci. Plant Nutrition 20, 149–159.
[45] Mierzwa-Hersztek, M., Gondek, K., Klimkowicz-Pawlas, A., Chmiel, M. J., Dziedzic, F., Taras, H. (2019). Assessment of soil quality after biochar application based on enzymatic activity and microbial composition. Int. Agrophys., 33, 331-336.
[46] Antonious, GF (2016). Soil amendments for agricultural production. Chapter 7 In: Organic Fertilizers: From Basic Concepts to Applied Outcomes, Book chapter, pages 157-187. Edited by Larramendy ML & Soloneski S, Published by Intech, Janeza Trdine 9, 51000 Rijeka, Croatia.
[47] Antonious, GF (2018). Biochar and animal manure impact on soil, crop yield and quality. Book Chapter In: Agricultural Waste. Edited by Prof. Anna Aladjadjiyan, National Biomass Association, Bulgaria & Published by Intech- Open Science Books, Janeza Trdine 9, 51000 Rijeka, Croatia.
[48] Hein, T. (2016). Note your grandpa’s poultry litter stockpile. Manure Manager, December issue, 10-12.
Cite This Article
  • APA Style

    George Fouad Antonious, Eric Todd Turley. (2020). Trace Elements Composition and Enzymes Activity of Soil Amended with Municipal Sewage Sludge at Three Locations in Kentucky. International Journal of Applied Agricultural Sciences, 6(5), 89-95. https://doi.org/10.11648/j.ijaas.20200605.11

    Copy | Download

    ACS Style

    George Fouad Antonious; Eric Todd Turley. Trace Elements Composition and Enzymes Activity of Soil Amended with Municipal Sewage Sludge at Three Locations in Kentucky. Int. J. Appl. Agric. Sci. 2020, 6(5), 89-95. doi: 10.11648/j.ijaas.20200605.11

    Copy | Download

    AMA Style

    George Fouad Antonious, Eric Todd Turley. Trace Elements Composition and Enzymes Activity of Soil Amended with Municipal Sewage Sludge at Three Locations in Kentucky. Int J Appl Agric Sci. 2020;6(5):89-95. doi: 10.11648/j.ijaas.20200605.11

    Copy | Download

  • @article{10.11648/j.ijaas.20200605.11,
      author = {George Fouad Antonious and Eric Todd Turley},
      title = {Trace Elements Composition and Enzymes Activity of Soil Amended with Municipal Sewage Sludge at Three Locations in Kentucky},
      journal = {International Journal of Applied Agricultural Sciences},
      volume = {6},
      number = {5},
      pages = {89-95},
      doi = {10.11648/j.ijaas.20200605.11},
      url = {https://doi.org/10.11648/j.ijaas.20200605.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20200605.11},
      abstract = {Biological monitoring is a sensitive indicator of soil ecological stress for early restoration. The addition of manure, such as sewage sludge (SS) to agricultural soil contributes to the enhancement of organic compounds. However, SS may contain toxic metals that potentially affect soil microbial growth and the enzymes they produce. Soil samples were collected from three agricultural locations in Kentucky (Adair, Meade, and Franklin Counties), from areas where municipal SS was applied as a soil amendment for commercial crop production. The objectives of this investigation were to: 1) assess the impact of mixing native agricultural soil with municipal SS on the activities of the three enzymes that hydrolyze urea (urease, urea amidohydrolase, EC 3.5.1.5), sucrose (invertase, β-D-fructofuranosidase), and p-nitrophenyl phosphate (acid and alkaline phosphatase) and 2) determine total microbial activity using the fluorescein diacetate reagent. The concentrations of Cr, Ni, Cu, Zn, Pb, and C content in soil mixed with SS in Franklin location were greater compared to Adair and Meade locations. The elevated soil urease and invertase activities (47 and 89%, respectively) as well as acid and alkaline phosphatase activities (23 and 26%, respectively) in soil amended with SS provided evidence of increased soil microbial population and the enzymes they produce.},
     year = {2020}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Trace Elements Composition and Enzymes Activity of Soil Amended with Municipal Sewage Sludge at Three Locations in Kentucky
    AU  - George Fouad Antonious
    AU  - Eric Todd Turley
    Y1  - 2020/09/23
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijaas.20200605.11
    DO  - 10.11648/j.ijaas.20200605.11
    T2  - International Journal of Applied Agricultural Sciences
    JF  - International Journal of Applied Agricultural Sciences
    JO  - International Journal of Applied Agricultural Sciences
    SP  - 89
    EP  - 95
    PB  - Science Publishing Group
    SN  - 2469-7885
    UR  - https://doi.org/10.11648/j.ijaas.20200605.11
    AB  - Biological monitoring is a sensitive indicator of soil ecological stress for early restoration. The addition of manure, such as sewage sludge (SS) to agricultural soil contributes to the enhancement of organic compounds. However, SS may contain toxic metals that potentially affect soil microbial growth and the enzymes they produce. Soil samples were collected from three agricultural locations in Kentucky (Adair, Meade, and Franklin Counties), from areas where municipal SS was applied as a soil amendment for commercial crop production. The objectives of this investigation were to: 1) assess the impact of mixing native agricultural soil with municipal SS on the activities of the three enzymes that hydrolyze urea (urease, urea amidohydrolase, EC 3.5.1.5), sucrose (invertase, β-D-fructofuranosidase), and p-nitrophenyl phosphate (acid and alkaline phosphatase) and 2) determine total microbial activity using the fluorescein diacetate reagent. The concentrations of Cr, Ni, Cu, Zn, Pb, and C content in soil mixed with SS in Franklin location were greater compared to Adair and Meade locations. The elevated soil urease and invertase activities (47 and 89%, respectively) as well as acid and alkaline phosphatase activities (23 and 26%, respectively) in soil amended with SS provided evidence of increased soil microbial population and the enzymes they produce.
    VL  - 6
    IS  - 5
    ER  - 

    Copy | Download

Author Information
  • Division of Environmental Studies, College of Agriculture, Communities, and the Environment, Kentucky State University, Frankfort, USA

  • Division of Environmental Studies, College of Agriculture, Communities, and the Environment, Kentucky State University, Frankfort, USA

  • Sections