<i>GhCOR27</i>, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress

Jianping Li; Xiaoyan Hao; Zumuremu Tuerxun; Xiaochun Chang; Shengqi Gao; Wenran Hu; Guo Chen; Quansheng Huang

doi:doi:10.11648/j.ijaas.20200601.11

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GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress

Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, Wenran Hu, Guo Chen, Quansheng Huang

Published in International Journal of Applied Agricultural Sciences (Volume 6, Issue 1)

Received: 8 December 2019 Accepted: 25 December 2019 Published: 8 January 2020

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Abstract

The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.

Published in	International Journal of Applied Agricultural Sciences (Volume 6, Issue 1)
DOI	10.11648/j.ijaas.20200601.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Gossypium hirsutum, Cold Stress, Cold-related Gene (COR), Virus-induced Gene Silencing (VIGS)

References

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[17]	Chen, L., et al. (2011) A novel cold-regulated gene, COR25, of Brassica napus is involved in plant response and tolerance to cold stress. Plant cell reports 30 (4): 463-471.
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APA Style

Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, et al. (2020). GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. International Journal of Applied Agricultural Sciences, 6(1), 1-6. https://doi.org/10.11648/j.ijaas.20200601.11

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ACS Style

Jianping Li; Xiaoyan Hao; Zumuremu Tuerxun; Xiaochun Chang; Shengqi Gao, et al. GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. Int. J. Appl. Agric. Sci. 2020, 6(1), 1-6. doi: 10.11648/j.ijaas.20200601.11

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AMA Style

Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, et al. GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. Int J Appl Agric Sci. 2020;6(1):1-6. doi: 10.11648/j.ijaas.20200601.11

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@article{10.11648/j.ijaas.20200601.11,
  author = {Jianping Li and Xiaoyan Hao and Zumuremu Tuerxun and Xiaochun Chang and Shengqi Gao and Wenran Hu and Guo Chen and Quansheng Huang},
  title = {GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {6},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ijaas.20200601.11},
  url = {https://doi.org/10.11648/j.ijaas.20200601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20200601.11},
  abstract = {The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.},
 year = {2020}
}

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TY  - JOUR
T1  - GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress
AU  - Jianping Li
AU  - Xiaoyan Hao
AU  - Zumuremu Tuerxun
AU  - Xiaochun Chang
AU  - Shengqi Gao
AU  - Wenran Hu
AU  - Guo Chen
AU  - Quansheng Huang
Y1  - 2020/01/08
PY  - 2020
N1  - https://doi.org/10.11648/j.ijaas.20200601.11
DO  - 10.11648/j.ijaas.20200601.11
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20200601.11
AB  - The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.
VL  - 6
IS  - 1
ER  -

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Author Information

Jianping Li

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Xiaoyan Hao

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Zumuremu Tuerxun

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Xiaochun Chang

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Shengqi Gao

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Wenran Hu

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Guo Chen

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China
Quansheng Huang

Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

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APA Style

Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, et al. (2020). GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. International Journal of Applied Agricultural Sciences, 6(1), 1-6. https://doi.org/10.11648/j.ijaas.20200601.11

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ACS Style

Jianping Li; Xiaoyan Hao; Zumuremu Tuerxun; Xiaochun Chang; Shengqi Gao, et al. GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. Int. J. Appl. Agric. Sci. 2020, 6(1), 1-6. doi: 10.11648/j.ijaas.20200601.11

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AMA Style

Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, et al. GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. Int J Appl Agric Sci. 2020;6(1):1-6. doi: 10.11648/j.ijaas.20200601.11

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@article{10.11648/j.ijaas.20200601.11,
  author = {Jianping Li and Xiaoyan Hao and Zumuremu Tuerxun and Xiaochun Chang and Shengqi Gao and Wenran Hu and Guo Chen and Quansheng Huang},
  title = {GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress},
  journal = {International Journal of Applied Agricultural Sciences},
  volume = {6},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ijaas.20200601.11},
  url = {https://doi.org/10.11648/j.ijaas.20200601.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20200601.11},
  abstract = {The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress
AU  - Jianping Li
AU  - Xiaoyan Hao
AU  - Zumuremu Tuerxun
AU  - Xiaochun Chang
AU  - Shengqi Gao
AU  - Wenran Hu
AU  - Guo Chen
AU  - Quansheng Huang
Y1  - 2020/01/08
PY  - 2020
N1  - https://doi.org/10.11648/j.ijaas.20200601.11
DO  - 10.11648/j.ijaas.20200601.11
T2  - International Journal of Applied Agricultural Sciences
JF  - International Journal of Applied Agricultural Sciences
JO  - International Journal of Applied Agricultural Sciences
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2469-7885
UR  - https://doi.org/10.11648/j.ijaas.20200601.11
AB  - The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.
VL  - 6
IS  - 1
ER  -

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