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GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress

Received: 8 December 2019     Accepted: 25 December 2019     Published: 8 January 2020
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Abstract

The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.

Published in International Journal of Applied Agricultural Sciences (Volume 6, Issue 1)
DOI 10.11648/j.ijaas.20200601.11
Page(s) 1-6
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Gossypium hirsutum, Cold Stress, Cold-related Gene (COR), Virus-induced Gene Silencing (VIGS)

References
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[2] Kargiotidou, A., et al. (2010) Cold acclimation and low temperature resistance in cotton: Gossypium hirsutum phospholipase Dα isoforms are differentially regulated by temperature and light. Journal of experimental botany 61 (11): 2991-3002.
[3] Thomashow, M. F. (1999) Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annual review of plant biology 50 (1): 571-599.
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  • APA Style

    Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, et al. (2020). GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. International Journal of Applied Agricultural Sciences, 6(1), 1-6. https://doi.org/10.11648/j.ijaas.20200601.11

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    ACS Style

    Jianping Li; Xiaoyan Hao; Zumuremu Tuerxun; Xiaochun Chang; Shengqi Gao, et al. GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. Int. J. Appl. Agric. Sci. 2020, 6(1), 1-6. doi: 10.11648/j.ijaas.20200601.11

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    AMA Style

    Jianping Li, Xiaoyan Hao, Zumuremu Tuerxun, Xiaochun Chang, Shengqi Gao, et al. GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress. Int J Appl Agric Sci. 2020;6(1):1-6. doi: 10.11648/j.ijaas.20200601.11

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  • @article{10.11648/j.ijaas.20200601.11,
      author = {Jianping Li and Xiaoyan Hao and Zumuremu Tuerxun and Xiaochun Chang and Shengqi Gao and Wenran Hu and Guo Chen and Quansheng Huang},
      title = {GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress},
      journal = {International Journal of Applied Agricultural Sciences},
      volume = {6},
      number = {1},
      pages = {1-6},
      doi = {10.11648/j.ijaas.20200601.11},
      url = {https://doi.org/10.11648/j.ijaas.20200601.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijaas.20200601.11},
      abstract = {The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - GhCOR27, Which Encodes a Cold-related Gene, Is Involved in Cotton Tolerance to Cold Stress
    AU  - Jianping Li
    AU  - Xiaoyan Hao
    AU  - Zumuremu Tuerxun
    AU  - Xiaochun Chang
    AU  - Shengqi Gao
    AU  - Wenran Hu
    AU  - Guo Chen
    AU  - Quansheng Huang
    Y1  - 2020/01/08
    PY  - 2020
    N1  - https://doi.org/10.11648/j.ijaas.20200601.11
    DO  - 10.11648/j.ijaas.20200601.11
    T2  - International Journal of Applied Agricultural Sciences
    JF  - International Journal of Applied Agricultural Sciences
    JO  - International Journal of Applied Agricultural Sciences
    SP  - 1
    EP  - 6
    PB  - Science Publishing Group
    SN  - 2469-7885
    UR  - https://doi.org/10.11648/j.ijaas.20200601.11
    AB  - The growth and development of crop plants are greatly affected by various abiotic stresses such as cold stress. Cold stress tolerance is triggered by a sensing mechanism, leading to signaling transduction in plants. Especially in the model species Arabidopsis (Arabidopsis thaliana) it was found that cold-related genes play important roles via cold stress. Cotton is thermophilic and sensitive to cold temperature during its development and growth. However, the mechanism of about cotton how to sense the cold response remains elusive. Here, we isolated a gene from cotton seedling cDNA, which phylogenetic analysis clustered it into the Cold-Related gene member and the protein sequences showed that its closest homolog is Arabidopsis AtCOR27, so we defined it as GhCOR27. Furthermore, the expression pattern analysis via real-time PCR used peculiar GhCOR27 primers demonstrated that GhCOR27 mRNA accumulated abundance was gradually induced at low temperature. In addition, the cotton seedlings of silenced-GhCOR27 through virus-induced gene silencing (VIGS) exhibited a more severe chilling injury phenotype at low temperature. These results suggested that GhCOR27 may play a role in response to cotton cold stress response.
    VL  - 6
    IS  - 1
    ER  - 

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Author Information
  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

  • Institute of Nuclear Technology and Biotechnology, Xinjiang Academy of Agricultural Sciences, Urumqi, China

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