Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites

Temitayo Omotunde Olowomofe; Toyin Funmilola Babalola; Oluwabusayo Odunola Oluyide; Aderonke Adeyanju

doi:doi:10.11648/j.fem.20190503.12

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Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites

Temitayo Omotunde Olowomofe, Toyin Funmilola Babalola, Oluwabusayo Odunola Oluyide, Aderonke Adeyanju

Published in Frontiers in Environmental Microbiology (Volume 5, Issue 3)

Received: 6 July 2019 Accepted: 26 August 2019 Published: 20 September 2019

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Abstract

This study evaluates cellulose degrading bacteria isolates from paper mill dumpsites and their cellulolytic activity. Forty-one cellulose-degrading bacteria (CDB) were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. The isolates were identified based on Morphology and Biochemical characterization while the identities of the potent cellulolytic bacteria isolates were confirmed by 16SrRNA sequencing. The optimum pH, temperature and incubation period for cellulose production by the CBD were determined. Isolates EFB3, ADB4, EFB1 and EFB4 exhibited the maximum zone of clearance around the colony with diameter of 50mm, 43mm, 44mm and 54mm and with the hydrolytic value of 10, 10.6, 8.8 and 7.6 respectively. The three selected bacteria with highest cellulose-degrading ability had 96% similarity with Enterobacter soli, Eubacterium ventriosum and Klebsiella michiganensis. Production of cellulase by the bacteria were influenced by environmental factors. Klebsiella michiganensis had optimum activity (38.5U/ml/min) after 72 hours while Enterobacter soli and Eubacterium ventriosum had optimum cellulase activity of 35.5U/ml/min and 32.2U/ml/min respectively after 48 hours. Enterobacter soli, Klebsiella michiganensis and Eubacterium ventriosum had optimum cellulase activity of 38U/ml/min, 35.6U/ml/min and 35.2U/ml/min respectively at pH7 while they recorded lower cellulase activity at acidic and alkaline pH ranges. Eubacterium ventriosum and Klebsiella michiganensis exhibited optimum activity of 25.12 U/ml/min and 20.55 U/ml/min respectively at 40°C. Enterobacter soli had the least cellulase activity with its optimum (15.02U/ml/min) at 35°C. The findings of this study revealed the abundance of bacteria equipped with the essential enzymatic capability to decompose cellulosic materials in the study area. These bacteria can also be used for mass production of cellulase.

Published in	Frontiers in Environmental Microbiology (Volume 5, Issue 3)
DOI	10.11648/j.fem.20190503.12
Page(s)	77-83
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cellulose, Congo-Red Agar, Cellulolytic Activity, Cellulose-degrading Bacteria (CDB)

References

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Temitayo Omotunde Olowomofe, Toyin Funmilola Babalola, Oluwabusayo Odunola Oluyide, Aderonke Adeyanju. (2019). Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites. Frontiers in Environmental Microbiology, 5(3), 77-83. https://doi.org/10.11648/j.fem.20190503.12

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Temitayo Omotunde Olowomofe; Toyin Funmilola Babalola; Oluwabusayo Odunola Oluyide; Aderonke Adeyanju. Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites. Front. Environ. Microbiol. 2019, 5(3), 77-83. doi: 10.11648/j.fem.20190503.12

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AMA Style

Temitayo Omotunde Olowomofe, Toyin Funmilola Babalola, Oluwabusayo Odunola Oluyide, Aderonke Adeyanju. Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites. Front Environ Microbiol. 2019;5(3):77-83. doi: 10.11648/j.fem.20190503.12

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@article{10.11648/j.fem.20190503.12,
  author = {Temitayo Omotunde Olowomofe and Toyin Funmilola Babalola and Oluwabusayo Odunola Oluyide and Aderonke Adeyanju},
  title = {Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites},
  journal = {Frontiers in Environmental Microbiology},
  volume = {5},
  number = {3},
  pages = {77-83},
  doi = {10.11648/j.fem.20190503.12},
  url = {https://doi.org/10.11648/j.fem.20190503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190503.12},
  abstract = {This study evaluates cellulose degrading bacteria isolates from paper mill dumpsites and their cellulolytic activity. Forty-one cellulose-degrading bacteria (CDB) were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. The isolates were identified based on Morphology and Biochemical characterization while the identities of the potent cellulolytic bacteria isolates were confirmed by 16SrRNA sequencing. The optimum pH, temperature and incubation period for cellulose production by the CBD were determined. Isolates EFB3, ADB4, EFB1 and EFB4 exhibited the maximum zone of clearance around the colony with diameter of 50mm, 43mm, 44mm and 54mm and with the hydrolytic value of 10, 10.6, 8.8 and 7.6 respectively. The three selected bacteria with highest cellulose-degrading ability had 96% similarity with Enterobacter soli, Eubacterium ventriosum and Klebsiella michiganensis. Production of cellulase by the bacteria were influenced by environmental factors. Klebsiella michiganensis had optimum activity (38.5U/ml/min) after 72 hours while Enterobacter soli and Eubacterium ventriosum had optimum cellulase activity of 35.5U/ml/min and 32.2U/ml/min respectively after 48 hours. Enterobacter soli, Klebsiella michiganensis and Eubacterium ventriosum had optimum cellulase activity of 38U/ml/min, 35.6U/ml/min and 35.2U/ml/min respectively at pH7 while they recorded lower cellulase activity at acidic and alkaline pH ranges. Eubacterium ventriosum and Klebsiella michiganensis exhibited optimum activity of 25.12 U/ml/min and 20.55 U/ml/min respectively at 40°C. Enterobacter soli had the least cellulase activity with its optimum (15.02U/ml/min) at 35°C. The findings of this study revealed the abundance of bacteria equipped with the essential enzymatic capability to decompose cellulosic materials in the study area. These bacteria can also be used for mass production of cellulase.},
 year = {2019}
}

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TY - JOUR
T1 - Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites
AU - Temitayo Omotunde Olowomofe
AU - Toyin Funmilola Babalola
AU - Oluwabusayo Odunola Oluyide
AU - Aderonke Adeyanju
Y1 - 2019/09/20
PY - 2019
N1 - https://doi.org/10.11648/j.fem.20190503.12
DO - 10.11648/j.fem.20190503.12
T2 - Frontiers in Environmental Microbiology
JF - Frontiers in Environmental Microbiology
JO - Frontiers in Environmental Microbiology
SP - 77
EP - 83
PB - Science Publishing Group
SN - 2469-8067
UR - https://doi.org/10.11648/j.fem.20190503.12
AB - This study evaluates cellulose degrading bacteria isolates from paper mill dumpsites and their cellulolytic activity. Forty-one cellulose-degrading bacteria (CDB) were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. The isolates were identified based on Morphology and Biochemical characterization while the identities of the potent cellulolytic bacteria isolates were confirmed by 16SrRNA sequencing. The optimum pH, temperature and incubation period for cellulose production by the CBD were determined. Isolates EFB3, ADB4, EFB1 and EFB4 exhibited the maximum zone of clearance around the colony with diameter of 50mm, 43mm, 44mm and 54mm and with the hydrolytic value of 10, 10.6, 8.8 and 7.6 respectively. The three selected bacteria with highest cellulose-degrading ability had 96% similarity with Enterobacter soli, Eubacterium ventriosum and Klebsiella michiganensis. Production of cellulase by the bacteria were influenced by environmental factors. Klebsiella michiganensis had optimum activity (38.5U/ml/min) after 72 hours while Enterobacter soli and Eubacterium ventriosum had optimum cellulase activity of 35.5U/ml/min and 32.2U/ml/min respectively after 48 hours. Enterobacter soli, Klebsiella michiganensis and Eubacterium ventriosum had optimum cellulase activity of 38U/ml/min, 35.6U/ml/min and 35.2U/ml/min respectively at pH7 while they recorded lower cellulase activity at acidic and alkaline pH ranges. Eubacterium ventriosum and Klebsiella michiganensis exhibited optimum activity of 25.12 U/ml/min and 20.55 U/ml/min respectively at 40°C. Enterobacter soli had the least cellulase activity with its optimum (15.02U/ml/min) at 35°C. The findings of this study revealed the abundance of bacteria equipped with the essential enzymatic capability to decompose cellulosic materials in the study area. These bacteria can also be used for mass production of cellulase.
VL - 5
IS - 3
ER -

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Author Information

Temitayo Omotunde Olowomofe

Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria
Toyin Funmilola Babalola

Department of Environmental Health Sciences, College of Health Science and Technology, Ijero-Ekiti, Nigeria
Oluwabusayo Odunola Oluyide

Department of Biomedical Engineering, College of Health Science and Technology, Ijero-Ekiti, Nigeria
Aderonke Adeyanju

Department of Microbiology, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria

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Plain Text BibTeX RIS

APA Style

Temitayo Omotunde Olowomofe, Toyin Funmilola Babalola, Oluwabusayo Odunola Oluyide, Aderonke Adeyanju. (2019). Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites. Frontiers in Environmental Microbiology, 5(3), 77-83. https://doi.org/10.11648/j.fem.20190503.12

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Temitayo Omotunde Olowomofe; Toyin Funmilola Babalola; Oluwabusayo Odunola Oluyide; Aderonke Adeyanju. Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites. Front. Environ. Microbiol. 2019, 5(3), 77-83. doi: 10.11648/j.fem.20190503.12

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AMA Style

Temitayo Omotunde Olowomofe, Toyin Funmilola Babalola, Oluwabusayo Odunola Oluyide, Aderonke Adeyanju. Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites. Front Environ Microbiol. 2019;5(3):77-83. doi: 10.11648/j.fem.20190503.12

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@article{10.11648/j.fem.20190503.12,
  author = {Temitayo Omotunde Olowomofe and Toyin Funmilola Babalola and Oluwabusayo Odunola Oluyide and Aderonke Adeyanju},
  title = {Isolation, Screening and Molecular Identification of Cellulose-degrading Bacteria from Paper and Pulp Mill Dumpsites},
  journal = {Frontiers in Environmental Microbiology},
  volume = {5},
  number = {3},
  pages = {77-83},
  doi = {10.11648/j.fem.20190503.12},
  url = {https://doi.org/10.11648/j.fem.20190503.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.fem.20190503.12},
  abstract = {This study evaluates cellulose degrading bacteria isolates from paper mill dumpsites and their cellulolytic activity. Forty-one cellulose-degrading bacteria (CDB) were isolated from soil samples from seven different paper and pulp mill dump sites using enrichment method. The isolates were identified based on Morphology and Biochemical characterization while the identities of the potent cellulolytic bacteria isolates were confirmed by 16SrRNA sequencing. The optimum pH, temperature and incubation period for cellulose production by the CBD were determined. Isolates EFB3, ADB4, EFB1 and EFB4 exhibited the maximum zone of clearance around the colony with diameter of 50mm, 43mm, 44mm and 54mm and with the hydrolytic value of 10, 10.6, 8.8 and 7.6 respectively. The three selected bacteria with highest cellulose-degrading ability had 96% similarity with Enterobacter soli, Eubacterium ventriosum and Klebsiella michiganensis. Production of cellulase by the bacteria were influenced by environmental factors. Klebsiella michiganensis had optimum activity (38.5U/ml/min) after 72 hours while Enterobacter soli and Eubacterium ventriosum had optimum cellulase activity of 35.5U/ml/min and 32.2U/ml/min respectively after 48 hours. Enterobacter soli, Klebsiella michiganensis and Eubacterium ventriosum had optimum cellulase activity of 38U/ml/min, 35.6U/ml/min and 35.2U/ml/min respectively at pH7 while they recorded lower cellulase activity at acidic and alkaline pH ranges. Eubacterium ventriosum and Klebsiella michiganensis exhibited optimum activity of 25.12 U/ml/min and 20.55 U/ml/min respectively at 40°C. Enterobacter soli had the least cellulase activity with its optimum (15.02U/ml/min) at 35°C. The findings of this study revealed the abundance of bacteria equipped with the essential enzymatic capability to decompose cellulosic materials in the study area. These bacteria can also be used for mass production of cellulase.},
 year = {2019}
}

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