Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki

Adeoti Olatunde Micheal; Akinbo Kehinde Oluwabusayo; Adesina David Ademola; Olufemi Samson Olutope

doi:doi:10.11648/j.ejcbs.20210701.12

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Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope

Published in European Journal of Clinical and Biomedical Sciences (Volume 7, Issue 1)

Received: 24 December 2019 Accepted: 16 January 2020 Published: 28 January 2021

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Abstract

Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjected to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10^-5 from river water followed by rain water with the lowest of 0.1×10⁵ CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.

Published in	European Journal of Clinical and Biomedical Sciences (Volume 7, Issue 1)
DOI	10.11648/j.ejcbs.20210701.12
Page(s)	6-11
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Fecal Coliform, Aseptically, Blasted, Enterobacter Kobeii, Water-borne Diseases, Well Water

References

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[7]	Pelezer, M. J., R. G. D & R. D. (2012). Microbiology (3^rd edition) M. C Grawhill book company Pp 143-767.
[8]	Shibata, T. Solo-Gabriele, H. M. Fleming, BLE, Elmir S. (2004) Monitoring marine recreational water quality using multiple microbial indicators in a urban tropical environment. Water Res 38: 3119-31.
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[16]	Ahmod, N. Z., Gupta, R. S., & Shah, H. N. (2011). J. M microbiology. Methods 87, 278-285 doi: 10./j.mimet2011.08.015.
[17]	WHO, U. (2000). Global water supply and sanitation assessment 2000 report. Geneva: World Health Organization.
[18]	WHO, U. (2014) Progress in drinking water and Sanitation: 2014 update. World.
[19]	Nuria, M. C., Rosyid, A., Sumantri, S. (2009). UjikandunganbakteriEscherichia colipada air minumisiulangdari depot air miniumisiulang di kabupatenrembang testing of Escherichia coli bacteria content drinking water refill from drinking water refill depot in Rembang sub-district. MEDIAGRO 5.
[20]	Boria, A. &Mitarb [2009]. Inter-countries epidemic as a result of contamination of drinking water with E. coli strain 0124. Abstract 2bi. Pp. 178-180.
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APA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. (2021). Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. European Journal of Clinical and Biomedical Sciences, 7(1), 6-11. https://doi.org/10.11648/j.ejcbs.20210701.12

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ACS Style

Adeoti Olatunde Micheal; Akinbo Kehinde Oluwabusayo; Adesina David Ademola; Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Eur. J. Clin. Biomed. Sci. 2021, 7(1), 6-11. doi: 10.11648/j.ejcbs.20210701.12

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AMA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Eur J Clin Biomed Sci. 2021;7(1):6-11. doi: 10.11648/j.ejcbs.20210701.12

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@article{10.11648/j.ejcbs.20210701.12,
  author = {Adeoti Olatunde Micheal and Akinbo Kehinde Oluwabusayo and Adesina David Ademola and Olufemi Samson Olutope},
  title = {Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {7},
  number = {1},
  pages = {6-11},
  doi = {10.11648/j.ejcbs.20210701.12},
  url = {https://doi.org/10.11648/j.ejcbs.20210701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20210701.12},
  abstract = {Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjected to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.},
 year = {2021}
}

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TY  - JOUR
T1  - Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki
AU  - Adeoti Olatunde Micheal
AU  - Akinbo Kehinde Oluwabusayo
AU  - Adesina David Ademola
AU  - Olufemi Samson Olutope
Y1  - 2021/01/28
PY  - 2021
N1  - https://doi.org/10.11648/j.ejcbs.20210701.12
DO  - 10.11648/j.ejcbs.20210701.12
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 6
EP  - 11
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20210701.12
AB  - Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjected to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.
VL  - 7
IS  - 1
ER  -

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Author Information

Adeoti Olatunde Micheal

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic, Saki, Nigeria
Akinbo Kehinde Oluwabusayo

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic, Saki, Nigeria
Adesina David Ademola

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic, Saki, Nigeria
Olufemi Samson Olutope

Department of Science Laboratory Technology (Microbiology Option), The Oke Ogun Polytechnic, Saki, Nigeria

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APA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. (2021). Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. European Journal of Clinical and Biomedical Sciences, 7(1), 6-11. https://doi.org/10.11648/j.ejcbs.20210701.12

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ACS Style

Adeoti Olatunde Micheal; Akinbo Kehinde Oluwabusayo; Adesina David Ademola; Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Eur. J. Clin. Biomed. Sci. 2021, 7(1), 6-11. doi: 10.11648/j.ejcbs.20210701.12

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AMA Style

Adeoti Olatunde Micheal, Akinbo Kehinde Oluwabusayo, Adesina David Ademola, Olufemi Samson Olutope. Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki. Eur J Clin Biomed Sci. 2021;7(1):6-11. doi: 10.11648/j.ejcbs.20210701.12

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@article{10.11648/j.ejcbs.20210701.12,
  author = {Adeoti Olatunde Micheal and Akinbo Kehinde Oluwabusayo and Adesina David Ademola and Olufemi Samson Olutope},
  title = {Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki},
  journal = {European Journal of Clinical and Biomedical Sciences},
  volume = {7},
  number = {1},
  pages = {6-11},
  doi = {10.11648/j.ejcbs.20210701.12},
  url = {https://doi.org/10.11648/j.ejcbs.20210701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20210701.12},
  abstract = {Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjected to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.},
 year = {2021}
}

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TY  - JOUR
T1  - Molecular Screening for Potential Pathogenic Organisms in Some Six Major Representative Sources of Water in Saki
AU  - Adeoti Olatunde Micheal
AU  - Akinbo Kehinde Oluwabusayo
AU  - Adesina David Ademola
AU  - Olufemi Samson Olutope
Y1  - 2021/01/28
PY  - 2021
N1  - https://doi.org/10.11648/j.ejcbs.20210701.12
DO  - 10.11648/j.ejcbs.20210701.12
T2  - European Journal of Clinical and Biomedical Sciences
JF  - European Journal of Clinical and Biomedical Sciences
JO  - European Journal of Clinical and Biomedical Sciences
SP  - 6
EP  - 11
PB  - Science Publishing Group
SN  - 2575-5005
UR  - https://doi.org/10.11648/j.ejcbs.20210701.12
AB  - Water security and safety is of vital concern in Saki and the rest of the world. The aim of this was to screen major sources of water in Saki of potential pathogens of public health importance. Water samples of six different water sources were purposely collected aseptically from six different locations in Saki (well water, river water, rain water, borehole water, sachet water and bottled water); these were subjected to standard laboratory analysis. Four highly populated plates were sent for molecular characterization for Polymerase Chain Reaction and species sequencing for molecular identification. The highest total colony count was 70.0×10-5 from river water followed by rain water with the lowest of 0.1×105 CFU from borehole water. Molecular analysis identified amplification of isolates genes at 15000 base pair for river water and well water samples. The analysis showed that Klebsiella pneumonia (MN208186) and Enterobacter kobeii (KM593928) were the organisms found in rain water and river water respectively. The resulting nucleotide sequences was blasted at NCBI for pairwise comparison with existing data in the GenBank and their similarity was noted. The pathogenicity of the two isolates have been well-documented, making some of the water sources tested so unfit for human usage. Large-scale treatment of water sources are recommended before consumption viz-a-vis hygienic practices around water bodies.
VL  - 7
IS  - 1
ER  -

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