The BACTEC MGIT 960 system is widely used for recovery of Mycobacterium tuberculosis, but high contamination rates compromise its diagnostic performance. Efforts to control contamination must balance elimination of contaminating organisms while preserving growth of tuberculous bacilli. Fosfomycin has activity against many bacteria responsible for culture contamination yet is inactive against Mycobacterium tuberculosis. Therefore, it holds potential as a selection agent for the culture of M. tuberculosis clinical samples with the MGIT system. In this study, we assess the ability of fosfomycin supplement to MGIT cultures of clinical sputum specimens. Sputum specimens collected at 62 diagnosis and treatment monitoring visits during a randomized controlled trial of high-dose rifampin (ClinicalTrials.gov NCT01408914) were split: one-half was treated as conventional and the other half was treated with 1mg of fosfomycin. The frequencies of contamination and of recovery of M. tuberculosis were compared using McNemar and Wilcoxon signed-rank test, respectively, with a=0.05. In the fosfomycin-treated samples, 2/62 (3.2%) yielded contaminated cultures while 12/62 (19.4%) of untreated cultures were contaminated (p<0.001, McNemar Test). Detection of M. tuberculosis was not significantly altered by the addition of fosfomycin (Time to positivity, p=0.576, Student’s T Test; log colony forming units), nor the Colony Forming Unit Counts (Wilcoxon signed-rank test p=0.671). These results suggest that the use of fosfomycin to control contamination does not affect the natural growth of MTB. Its use could result in a significant improvement in recovery of M. tuberculosis in MGIT.
| Published in | European Journal of Clinical and Biomedical Sciences (Volume 4, Issue 3) |
| DOI | 10.11648/j.ejcbs.20180403.13 |
| Page(s) | 51-54 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2018. Published by Science Publishing Group |
Fosfomycin, Culture Contamination, Tuberculosis, BACTEC MGITTM 960
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APA Style
Roger Ivan Calderon Espinoza, Maria Belen Arriaga Gutierrez, Kattya Lopez Tamara, Nadia Nilda Barreda Ponce, Carole Diane Mitnick, et al. (2018). Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System. European Journal of Clinical and Biomedical Sciences, 4(3), 51-54. https://doi.org/10.11648/j.ejcbs.20180403.13
ACS Style
Roger Ivan Calderon Espinoza; Maria Belen Arriaga Gutierrez; Kattya Lopez Tamara; Nadia Nilda Barreda Ponce; Carole Diane Mitnick, et al. Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System. Eur. J. Clin. Biomed. Sci. 2018, 4(3), 51-54. doi: 10.11648/j.ejcbs.20180403.13
AMA Style
Roger Ivan Calderon Espinoza, Maria Belen Arriaga Gutierrez, Kattya Lopez Tamara, Nadia Nilda Barreda Ponce, Carole Diane Mitnick, et al. Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System. Eur J Clin Biomed Sci. 2018;4(3):51-54. doi: 10.11648/j.ejcbs.20180403.13
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Download@article{10.11648/j.ejcbs.20180403.13,
author = {Roger Ivan Calderon Espinoza and Maria Belen Arriaga Gutierrez and Kattya Lopez Tamara and Nadia Nilda Barreda Ponce and Carole Diane Mitnick and Geraint Rhys Davies and David John Coleman},
title = {Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System},
journal = {European Journal of Clinical and Biomedical Sciences},
volume = {4},
number = {3},
pages = {51-54},
doi = {10.11648/j.ejcbs.20180403.13},
url = {https://doi.org/10.11648/j.ejcbs.20180403.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20180403.13},
abstract = {The BACTEC MGIT 960 system is widely used for recovery of Mycobacterium tuberculosis, but high contamination rates compromise its diagnostic performance. Efforts to control contamination must balance elimination of contaminating organisms while preserving growth of tuberculous bacilli. Fosfomycin has activity against many bacteria responsible for culture contamination yet is inactive against Mycobacterium tuberculosis. Therefore, it holds potential as a selection agent for the culture of M. tuberculosis clinical samples with the MGIT system. In this study, we assess the ability of fosfomycin supplement to MGIT cultures of clinical sputum specimens. Sputum specimens collected at 62 diagnosis and treatment monitoring visits during a randomized controlled trial of high-dose rifampin (ClinicalTrials.gov NCT01408914) were split: one-half was treated as conventional and the other half was treated with 1mg of fosfomycin. The frequencies of contamination and of recovery of M. tuberculosis were compared using McNemar and Wilcoxon signed-rank test, respectively, with a=0.05. In the fosfomycin-treated samples, 2/62 (3.2%) yielded contaminated cultures while 12/62 (19.4%) of untreated cultures were contaminated (pM. tuberculosis was not significantly altered by the addition of fosfomycin (Time to positivity, p=0.576, Student’s T Test; log colony forming units), nor the Colony Forming Unit Counts (Wilcoxon signed-rank test p=0.671). These results suggest that the use of fosfomycin to control contamination does not affect the natural growth of MTB. Its use could result in a significant improvement in recovery of M. tuberculosis in MGIT.},
year = {2018}
}
TY - JOUR T1 - Fosfomycin to Control Contamination in M. tuberculosis Culture in BACTEC MGIT 960 System AU - Roger Ivan Calderon Espinoza AU - Maria Belen Arriaga Gutierrez AU - Kattya Lopez Tamara AU - Nadia Nilda Barreda Ponce AU - Carole Diane Mitnick AU - Geraint Rhys Davies AU - David John Coleman Y1 - 2018/09/12 PY - 2018 N1 - https://doi.org/10.11648/j.ejcbs.20180403.13 DO - 10.11648/j.ejcbs.20180403.13 T2 - European Journal of Clinical and Biomedical Sciences JF - European Journal of Clinical and Biomedical Sciences JO - European Journal of Clinical and Biomedical Sciences SP - 51 EP - 54 PB - Science Publishing Group SN - 2575-5005 UR - https://doi.org/10.11648/j.ejcbs.20180403.13 AB - The BACTEC MGIT 960 system is widely used for recovery of Mycobacterium tuberculosis, but high contamination rates compromise its diagnostic performance. Efforts to control contamination must balance elimination of contaminating organisms while preserving growth of tuberculous bacilli. Fosfomycin has activity against many bacteria responsible for culture contamination yet is inactive against Mycobacterium tuberculosis. Therefore, it holds potential as a selection agent for the culture of M. tuberculosis clinical samples with the MGIT system. In this study, we assess the ability of fosfomycin supplement to MGIT cultures of clinical sputum specimens. Sputum specimens collected at 62 diagnosis and treatment monitoring visits during a randomized controlled trial of high-dose rifampin (ClinicalTrials.gov NCT01408914) were split: one-half was treated as conventional and the other half was treated with 1mg of fosfomycin. The frequencies of contamination and of recovery of M. tuberculosis were compared using McNemar and Wilcoxon signed-rank test, respectively, with a=0.05. In the fosfomycin-treated samples, 2/62 (3.2%) yielded contaminated cultures while 12/62 (19.4%) of untreated cultures were contaminated (pM. tuberculosis was not significantly altered by the addition of fosfomycin (Time to positivity, p=0.576, Student’s T Test; log colony forming units), nor the Colony Forming Unit Counts (Wilcoxon signed-rank test p=0.671). These results suggest that the use of fosfomycin to control contamination does not affect the natural growth of MTB. Its use could result in a significant improvement in recovery of M. tuberculosis in MGIT. VL - 4 IS - 3 ER -
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