The presence of ESBLs in many Gram negative strains are of serious concern, since these organisms are the most common cause of different human infections. ESBL positive phenotypically were tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, TEM and SHV genes, then the amplicons were sequenced to characterized gene content. The presence of CTX-M, TEM and SHV genes was confirmed in 65/88 (73.9%) of the isolates. The ESBL genes were detected in 47 Escherichia coli, 14 Klebsiellapneumoniae, 2 Proteus mirabilis, 1 Serratiaodotifera and 1 Enterobactersakasaki. The nucleotide sequences were subjected to BLAST for sequences similarity and homology.
| Published in | European Journal of Clinical and Biomedical Sciences (Volume 3, Issue 6) |
| DOI | 10.11648/j.ejcbs.20170306.11 |
| Page(s) | 101-108 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
E. coli, K. Pneumoniae, ESBLs, CTX-M Genes, TEM Gene, SHV Genes, Sudan
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APA Style
Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. (2017). Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital. European Journal of Clinical and Biomedical Sciences, 3(6), 101-108. https://doi.org/10.11648/j.ejcbs.20170306.11
ACS Style
Abd Elrahman Mustafa Abd Elrahman Osman; Shingray Osman Hashim; Mohammed Abdall Musa; Omer Mohammed Tahir. Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital. Eur. J. Clin. Biomed. Sci. 2017, 3(6), 101-108. doi: 10.11648/j.ejcbs.20170306.11
AMA Style
Abd Elrahman Mustafa Abd Elrahman Osman, Shingray Osman Hashim, Mohammed Abdall Musa, Omer Mohammed Tahir. Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital. Eur J Clin Biomed Sci. 2017;3(6):101-108. doi: 10.11648/j.ejcbs.20170306.11
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Download@article{10.11648/j.ejcbs.20170306.11,
author = {Abd Elrahman Mustafa Abd Elrahman Osman and Shingray Osman Hashim and Mohammed Abdall Musa and Omer Mohammed Tahir},
title = {Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital},
journal = {European Journal of Clinical and Biomedical Sciences},
volume = {3},
number = {6},
pages = {101-108},
doi = {10.11648/j.ejcbs.20170306.11},
url = {https://doi.org/10.11648/j.ejcbs.20170306.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ejcbs.20170306.11},
abstract = {The presence of ESBLs in many Gram negative strains are of serious concern, since these organisms are the most common cause of different human infections. ESBL positive phenotypically were tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, TEM and SHV genes, then the amplicons were sequenced to characterized gene content. The presence of CTX-M, TEM and SHV genes was confirmed in 65/88 (73.9%) of the isolates. The ESBL genes were detected in 47 Escherichia coli, 14 Klebsiellapneumoniae, 2 Proteus mirabilis, 1 Serratiaodotifera and 1 Enterobactersakasaki. The nucleotide sequences were subjected to BLAST for sequences similarity and homology.},
year = {2017}
}
TY - JOUR T1 - Detection of CTX-M, TEM and SHV Genes in Gram Negative Bacteria Isolated from Nosocomial Patients at Port Sudan Teaching Hospital AU - Abd Elrahman Mustafa Abd Elrahman Osman AU - Shingray Osman Hashim AU - Mohammed Abdall Musa AU - Omer Mohammed Tahir Y1 - 2017/11/15 PY - 2017 N1 - https://doi.org/10.11648/j.ejcbs.20170306.11 DO - 10.11648/j.ejcbs.20170306.11 T2 - European Journal of Clinical and Biomedical Sciences JF - European Journal of Clinical and Biomedical Sciences JO - European Journal of Clinical and Biomedical Sciences SP - 101 EP - 108 PB - Science Publishing Group SN - 2575-5005 UR - https://doi.org/10.11648/j.ejcbs.20170306.11 AB - The presence of ESBLs in many Gram negative strains are of serious concern, since these organisms are the most common cause of different human infections. ESBL positive phenotypically were tested for the presence of ESBL encoding genes using PCR with specific primers for the detection of CTX-M, TEM and SHV genes, then the amplicons were sequenced to characterized gene content. The presence of CTX-M, TEM and SHV genes was confirmed in 65/88 (73.9%) of the isolates. The ESBL genes were detected in 47 Escherichia coli, 14 Klebsiellapneumoniae, 2 Proteus mirabilis, 1 Serratiaodotifera and 1 Enterobactersakasaki. The nucleotide sequences were subjected to BLAST for sequences similarity and homology. VL - 3 IS - 6 ER -
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