In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.
| Published in | Ecology and Evolutionary Biology (Volume 4, Issue 4) |
| DOI | 10.11648/j.eeb.20190404.12 |
| Page(s) | 55-59 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Cross-amplification, Genetic Marker, Scallop, Species Identification, Simple Sequence Repeats
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APA Style
Min Hui. (2019). Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae. Ecology and Evolutionary Biology, 4(4), 55-59. https://doi.org/10.11648/j.eeb.20190404.12
ACS Style
Min Hui. Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae. Ecol. Evol. Biol. 2019, 4(4), 55-59. doi: 10.11648/j.eeb.20190404.12
AMA Style
Min Hui. Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae. Ecol Evol Biol. 2019;4(4):55-59. doi: 10.11648/j.eeb.20190404.12
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Download@article{10.11648/j.eeb.20190404.12,
author = {Min Hui},
title = {Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae},
journal = {Ecology and Evolutionary Biology},
volume = {4},
number = {4},
pages = {55-59},
doi = {10.11648/j.eeb.20190404.12},
url = {https://doi.org/10.11648/j.eeb.20190404.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.eeb.20190404.12},
abstract = {In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.},
year = {2019}
}
TY - JOUR T1 - Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae AU - Min Hui Y1 - 2019/11/07 PY - 2019 N1 - https://doi.org/10.11648/j.eeb.20190404.12 DO - 10.11648/j.eeb.20190404.12 T2 - Ecology and Evolutionary Biology JF - Ecology and Evolutionary Biology JO - Ecology and Evolutionary Biology SP - 55 EP - 59 PB - Science Publishing Group SN - 2575-3762 UR - https://doi.org/10.11648/j.eeb.20190404.12 AB - In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification. VL - 4 IS - 4 ER -
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