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Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae

Received: 3 September 2019     Accepted: 24 September 2019     Published: 7 November 2019
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Abstract

In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.

Published in Ecology and Evolutionary Biology (Volume 4, Issue 4)
DOI 10.11648/j.eeb.20190404.12
Page(s) 55-59
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Cross-amplification, Genetic Marker, Scallop, Species Identification, Simple Sequence Repeats

References
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[3] Slabbert, R., A. E. Bester, and M. E. D'Amato (2017). Analyses of genetic diversity and parentage within a South African hatchery of the abalone Haliotis midae Linnaeus using microsatellite markers. Journal of Shellfish Research 28, 369–375.
[4] Xu, X., G. Wang, C. Zeng and S. Li (2018) Parentage determination of the mud crab Scylla paramamosain using microsatellite markers. Aquaculture Research 49, 217–221.
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[6] Hedgecock, D., G. Li, S. Hubert, K. Bucklin and V. Ribes (2004). Widespread null alleles and poor cross-species amplification of microsatellite DNA loci cloned from the Pacific Oyster, Crassostrea gigas. Journal of Shellfish Research 23, 379–385.
[7] Díaz-viloria, N., R. Pérez-enríquez, G. Fiore-amaral, R. S. Burton and P. Cruz (2008). Isolation and cross-amplification of microsatellites in pink abalone (Haliotis corrugata). Molecular Ecology Resources 8, 701–703.
[8] Zhan A., Z. Bao, X. Hu, S. Wang, W. Peng, M. Wang, J. Hu, C. Liang and Z. Yue (2008). Characterization of 95 novel microsatellite markers for Zhikong scallop (Chlamys farreri) using FIASCO-colony hybridization and EST database mining. Fisheries Science 74, 516–526.
[9] Sun C., A. Zhan, M. Hui, W. Lu, X. Hu, J. Hu and Z. Bao (2007). Characterization of novel microsatellite markers from the Yesso scallop Mizuhopecten yessoensis. Molecular Ecology Notes 7, 106–108.
[10] Hui M., Z. Bao, A. Zhan, X. Hu, W. Lu, D. Chang and J. Hu (2006). Ten polymorphic dinucleotide microsatellite markers of the noble scallop Chlamys nobilis. Molecular Ecology Notes 6, 1033–1035.
[11] Meng X, Wang X, Guo X, M. Chen, Y. Zhang, H. Wang, Y. Chang and X. Qiu (2011). Characterization of novel polymorphic microsatellite markers from the cDNA library of Japanese scallop Mizuhopecten yessoensis and cross-species amplification in bay scallop Argopecten irradians. Journal of Genetics 90, 62–65.
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[13] Zhan, A. B., Z. M. Bao, B. Yao, X. L. Wang, M. Hui and J. J. Hu (2006). Polymorphic microsatellite markers in the Zhikong scallop Chlamys farreri. Molecular Ecology Notes 6, 127–129.
[14] Zhan A., Z. Bao, X. Hu, M. Hui, M. Wang, W. Peng, H. Zhao and J. Hu (2007). Isolation and characterization of 150 novel microsatellite markers for Zhikong scallop (Chlamys farreri). Molecular Ecology Notes 7, 1015–1022.
[15] Zhan A., J. Hu, X.. Wang, W. Lu, M. Hui and Z. Bao (2006). A panel of polymorphic EST-derived microsatellite loci for the bay scallop (Argopecten irradians). Journal of Molluscan Studies 72, 436–438.
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    Min Hui. (2019). Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae. Ecology and Evolutionary Biology, 4(4), 55-59. https://doi.org/10.11648/j.eeb.20190404.12

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    ACS Style

    Min Hui. Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae. Ecol. Evol. Biol. 2019, 4(4), 55-59. doi: 10.11648/j.eeb.20190404.12

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    AMA Style

    Min Hui. Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae. Ecol Evol Biol. 2019;4(4):55-59. doi: 10.11648/j.eeb.20190404.12

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  • @article{10.11648/j.eeb.20190404.12,
      author = {Min Hui},
      title = {Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae},
      journal = {Ecology and Evolutionary Biology},
      volume = {4},
      number = {4},
      pages = {55-59},
      doi = {10.11648/j.eeb.20190404.12},
      url = {https://doi.org/10.11648/j.eeb.20190404.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.eeb.20190404.12},
      abstract = {In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.},
     year = {2019}
    }
    

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    T1  - Cross-species Amplification of Scallop Microsatellites in the Family Pectinidae
    AU  - Min Hui
    Y1  - 2019/11/07
    PY  - 2019
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    DO  - 10.11648/j.eeb.20190404.12
    T2  - Ecology and Evolutionary Biology
    JF  - Ecology and Evolutionary Biology
    JO  - Ecology and Evolutionary Biology
    SP  - 55
    EP  - 59
    PB  - Science Publishing Group
    SN  - 2575-3762
    UR  - https://doi.org/10.11648/j.eeb.20190404.12
    AB  - In the present study, 103 microsatellite markers were selected for cross-species amplification in four marine economic scallop species (Argopecten irradians, Chlamys farreri, Chlamys nobilis, and Mizuhopecten yessoensis) belonging to the family Pectinidae. As a result, in the 72 markers originated from C. farreri, 12 showed amplification products in C. nobilis, 12 in A. irradians and 11 in M. yessoensis. In the 12 markers developed from M. yessoensis, four markers could be amplified successfully in A. irradians, three in C. farreri, and one in C. nobilis. In the nine markers isolated from C. nobilis, two markers could be cross-amplified in A. irradians and one in M. yessoensis, but no marker was likely to be useful in C. farreri. In the nine markers of A. irradians, one marker was potentially workable for C. farreri, one in M. yessoensis, but none in C. nobilis. When the microsatellites were cross-species amplified, most of the PCR products showed low yield and ambiguous bands, while the numbers of alleles also decreased. Finally, three markers (CFMS016, CFCD131 and CFE04) were revealed to be successfully transferred among the four species providing candidate markers for ecological study of scallops, while most of the other markers were unique for one species with poor cross-species amplification, which might be useful for species identification.
    VL  - 4
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Author Information
  • Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China

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