@article{10.11648/j.cnn.20220604.11,
  author = {Jean Pierre Omam Omam and Andre Hamadou and Samuel Mbouh and Juliette Koube and Dang Bouba Kadjou and Mireille Delphine Ze Minkoulou and Fleur Clarisse Moto Okomolo and Elisabeth Ngo Bum},
  title = {Anxiolytic and Antioxydant Effects of Aqueous Extract of Hiptis spicigera Lam in Mice Exposed to Classical Paradigms and Chronic Immobilisation Test},
  journal = {Clinical Neurology and Neuroscience},
  volume = {6},
  number = {4},
  pages = {50-61},
  doi = {10.11648/j.cnn.20220604.11},
  url = {https://doi.org/10.11648/j.cnn.20220604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cnn.20220604.11},
  abstract = {Anxiety is defined as an exaggerated feeling of apprehension, uncertainty and fear. The pharmacological treatments used for this disease show many side effects, which has limited their use by the patients. However, it is worth mentioning that in Africa medicinal plants are invaluable resources for the vast majority of rural populations, where more than 80% use them for their primary health care. This reality could justify the use of plants in African traditional medicine to treat neuropsychiatric diseases. The aim of this study is to assess the anxiolytic properties of Hyptis spicigera Lam leaves decoction on chronic immobilisation after treated by classical tests as EPM, Open Field and Hole Board. In order to assess the anxiolytic activity of the decoction on acute and chronic anxiety, the mice were divided by groups and treated with distilled water, Diazepam and different doses of the decoction (22.5mg/kg, 56.25mg/kg, 112.5mg/kg and 225mg/kg). Finally, some oxidative stress parameters such like catalase, sulfoxide dismutase, reduced glutathione, nitrite and malondialdehyde were measured. Concerning EPM test, the figure 1A and B showed a significant increase respectively of number of entries 6.8±1 and time spent in the open arms 94 ± 22.74 s from the negative control to 13.6 ± 2.3 and 210 ± 11 s in 225mg/kg dose of plant. Also, the figure 1C showed a significant decrease of the percentage of time spent in the closed arms from 68.7 to 30%. These parameters showed the reduction of the level of anxiety in these mice. The Open Field and Hole Board tests, by their parameters also showed that the Hyptis spicigera decoction would have anxiolytic properties. This could be justified may be by the presence of secondary metabolites such as saponins and flavonoids. The assay of oxidative stress markers showed that mice group which received the decoction and diazepam, had elevated catalase, sulfoxide dismutase, reduced glutathione and nitrites, but their malondialdehyde was low. The results of our study have shown that the decoction of Hyptis spicigera Lam would have anxiolytic and antioxydant properties.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Anxiolytic and Antioxydant Effects of Aqueous Extract of Hiptis spicigera Lam in Mice Exposed to Classical Paradigms and Chronic Immobilisation Test
AU  - Jean Pierre Omam Omam
AU  - Andre Hamadou
AU  - Samuel Mbouh
AU  - Juliette Koube
AU  - Dang Bouba Kadjou
AU  - Mireille Delphine Ze Minkoulou
AU  - Fleur Clarisse Moto Okomolo
AU  - Elisabeth Ngo Bum
Y1  - 2022/12/08
PY  - 2022
N1  - https://doi.org/10.11648/j.cnn.20220604.11
DO  - 10.11648/j.cnn.20220604.11
T2  - Clinical Neurology and Neuroscience
JF  - Clinical Neurology and Neuroscience
JO  - Clinical Neurology and Neuroscience
SP  - 50
EP  - 61
PB  - Science Publishing Group
SN  - 2578-8930
UR  - https://doi.org/10.11648/j.cnn.20220604.11
AB  - Anxiety is defined as an exaggerated feeling of apprehension, uncertainty and fear. The pharmacological treatments used for this disease show many side effects, which has limited their use by the patients. However, it is worth mentioning that in Africa medicinal plants are invaluable resources for the vast majority of rural populations, where more than 80% use them for their primary health care. This reality could justify the use of plants in African traditional medicine to treat neuropsychiatric diseases. The aim of this study is to assess the anxiolytic properties of Hyptis spicigera Lam leaves decoction on chronic immobilisation after treated by classical tests as EPM, Open Field and Hole Board. In order to assess the anxiolytic activity of the decoction on acute and chronic anxiety, the mice were divided by groups and treated with distilled water, Diazepam and different doses of the decoction (22.5mg/kg, 56.25mg/kg, 112.5mg/kg and 225mg/kg). Finally, some oxidative stress parameters such like catalase, sulfoxide dismutase, reduced glutathione, nitrite and malondialdehyde were measured. Concerning EPM test, the figure 1A and B showed a significant increase respectively of number of entries 6.8±1 and time spent in the open arms 94 ± 22.74 s from the negative control to 13.6 ± 2.3 and 210 ± 11 s in 225mg/kg dose of plant. Also, the figure 1C showed a significant decrease of the percentage of time spent in the closed arms from 68.7 to 30%. These parameters showed the reduction of the level of anxiety in these mice. The Open Field and Hole Board tests, by their parameters also showed that the Hyptis spicigera decoction would have anxiolytic properties. This could be justified may be by the presence of secondary metabolites such as saponins and flavonoids. The assay of oxidative stress markers showed that mice group which received the decoction and diazepam, had elevated catalase, sulfoxide dismutase, reduced glutathione and nitrites, but their malondialdehyde was low. The results of our study have shown that the decoction of Hyptis spicigera Lam would have anxiolytic and antioxydant properties.
VL  - 6
IS  - 4
ER  - 

Copy | Download