Advances in bioreactor technologies are transforming sustainable aquaculture water treatment by improving pollutant removal and supporting environmental conservation and resource recovery. Recirculating Aquaculture Systems (RAS) represent a leading sustainable approach by integrating physical, chemical, and biological treatment processes to recycle water within the system, minimizing freshwater consumption and effluent discharge. Innovative biological systems integrated with Recirculating Aquaculture Systems (RAS) including Moving Bed Biofilm Reactors, membrane bioreactors, anaerobic digesters, photobioreactors, and biofloc efficiently reduce nitrogen, phosphorus, organic matter, and other pollutants using diverse microbial communities without harmful chemicals. Recent developments feature microalgae cultivation for carbon capture and nutrient recycling, nanotechnology to boost microbial performance, and hybrid treatment methods for enhanced effectiveness. While Moving Bed Biofilm Reactors offer high ammonia and organic removal in compact setups, anaerobic bioreactors provide cost-effective nitrate reduction, and constructed wetlands effectively remove organics and phosphorus with more space needs. These bioreactors technology enhance aquaculture sustainability by reducing pollutant loads, mitigating eutrophication risks, and improving fish health through optimized water quality. Despite operational and cost challenges, these technologies promote water reuse, lower pollutant discharge, and enable circular economy practices like bioenergy production. Future research focuses on tailored, integrated treatments, engineered microbes, and resource-loop closing frameworks to bolster sustainability, regulatory compliance, and economic viability in intensive aquaculture. The aim of this review article is to examine recent innovations and developments in bioreactor technologies applied to aquaculture wastewater treatment.
Published in | American Journal of Bioscience and Bioengineering (Volume 13, Issue 5) |
DOI | 10.11648/j.bio.20251305.11 |
Page(s) | 92-98 |
Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
Copyright |
Copyright © The Author(s), 2025. Published by Science Publishing Group |
Bioreactor Technologies, Sustainable Aquaculture, Recirculating Aquaculture Systems (RAS), Nutrient Removal, Microbial Treatment, Resource Recovery, Circular Economy
COD | Chemical Oxygen Demand |
MBBRs | Moving Bed Biofilm Reactors |
MBRs | Membrane Bioreactors |
RAS | Recirculating Aquaculture Systems |
SBRs | Sequencing Batch Reactors |
SBRs | Sequencing Batch Reactors |
TAN | Total Ammonia Nitrogen |
[1] | A. B. Dauda, A. Ajadi, A. S. Tola-Fabunmi, and A. O. Akinwole, “Waste production in aquaculture: Sources, components and managements in different culture systems,” Aquac. Fish., vol. 4, no. 3, pp. 81–88, 2019, |
[2] | L. Q. Viet, T. N. Hai, N. T. Em, D. T. M. Han, T. Emi, and N. Q. Thinh, “Effect of different biofilter media in recirculating aquaculture system application for whiteleg shrimp (Litopenaeus vannamei Boone, 1931) farming,” AACL Bioflux, vol. 18, no. 1, pp. 433–443, 2025. |
[3] | S. Tabrett, I. Ramsay, B. Paterson, and M. A. Burford, “A review of the benefits and limitations of waste nutrient treatment in aquaculture pond facilities,” Rev. Aquac., vol. 16, no. 4, pp. 1766–1786, 2024, |
[4] | S. Naz et al., “Innovative Approaches in Wastewater Management: A Comprehensive Review,” Int. J. Curr. Microbiol. Appl. Sci., vol. 14, no. 3, pp. 140–164, 2025, |
[5] | Z. Li et al., “Strengthening and microbial regulation mechanism of Bacillus on purification device for grass carp culture wastewater,” Front. Environ. Sci., vol. 11, no. January, pp. 1–12, 2023, |
[6] | S. Saini, S. Tewari, J. Dwivedi, and V. Sharma, “Biofilm-mediated wastewater treatment: a comprehensive review,” Mater. Adv., vol. 4, no. 6, pp. 1415–1443, 2023, |
[7] | M. S. Najafinejad, S. Chianese, A. Fenti, P. Iovino, and D. Musmarra, “Application of Electrochemical Oxidation for Water and Wastewater Treatment: An Overview,” Molecules, vol. 28, no. 10, pp. 1–24, 2023, |
[8] | R. Ben-Asher, Y. Gendel, and O. Lahav, “Electrochemical applications in RAS: A review,” Rev. Aquac., vol. 16, no. 1, pp. 86–105, 2024, |
[9] | N. K. Yadav et al., “The role of biofloc technology in sustainable aquaculture: nutritional insights and system efficiency,” Blue Biotechnol., vol. 2, no. 1, 2025, |
[10] | B. Delaide, H. Monsees, A. Gross, and S. Goddek, “Aerobic and Anaerobic Treatments for Aquaponic Sludge Reduction and Mineralisation,” Aquaponics Food Prod. Syst. Comb. Aquac. Hydroponic Prod. Technol. Futur., pp. 247–266, 2019, |
[11] | O. M. Wrightwood, M. E. Hattaway, T. M. Young, and H. N. Bischel, “Assessment of Woodchip Bioreactor Characteristics and Their Influences on Joint Nitrate and Pesticide Removal,” ACS Environ. Sci. Technol. Water, vol. 2, no. 1, pp. 106–116, 2022, |
[12] | D. hui Liang, Y. Hu, D. Liang, J. Chenga, and Y. Chena, “Bioaugmentation of Moving Bed Biofilm Reactor (MBBR) with Achromobacter JL9 for enhanced sulfamethoxazole (SMX) degradation in aquaculture wastewater,” Ecotoxicol. Environ. Saf., vol. 207, no. September 2020, p. 111258, 2021, |
[13] | L. H. Anh and V. T. K. Khuyen, “Biotreatment of aquaculture wastewater with chlorella sp. in tubular photo-bioreactors,” Int. J. Sci. Technol. Res., vol. 9, no. 4, pp. 1008–1013, 2020. |
[14] | P. Jakhwal et al., “Nutrient removal and biomass production of marine microalgae cultured in recirculating aquaculture systems (RAS) water with low phosphate concentration,” J. Environ. Manage., vol. 358, no. April, p. 120859, 2024, |
[15] | H. R. Mian, S. H. Hussain, and Gul-E-hina, “Optimization of sequencing batch reactor for wastewater treatment using chemically enhanced primary treatment as a pre-treatment,” Water SA, vol. 44, no. 3, pp. 399–405, 2018, |
[16] | C. Kern and R. Boopathy, “Use of Sequencing Batch Reactor in the Treatment of Shrimp Aquaculture Wastewater,” J. Water Sustain., vol. 2, no. 4, pp. 221–232, 2012. |
[17] | B. Raza, Z. Zheng, and W. Yang, “A Review on Biofloc System Technology, History, Types, and Future Economical Perceptions in Aquaculture,” Animals, vol. 14, no. 10, 2024, |
[18] | M. Aliyu-paiko, A. K. Mohammed, M. Jibrin, and M. Road, “JOURNAL OF BIOCHEMISTRY, MICROBIOLOGY Biofloc Technology as a Sustainable Alternative for Managing Aquaculture Wastewater,” vol. 12, no. 1, pp. 44–53, 2024. |
[19] | H. M. Hwihy, A. F. Zeina, and K. A. El-Damhougy, “Influence of biofloc technology on economic evaluation of culturing oreochromis niloticus reared at different stocking densities and feeding rates.,” Egypt. J. Aquat. Biol. Fish., vol. 25, no. 1, pp. 737–748, 2021, |
[20] | Z. Li et al., “Water Treatment Effect, Microbial Community Structure, and Metabolic Characteristics in a Field-Scale Aquaculture Wastewater Treatment System,” Front. Microbiol., vol. 11, no. June, pp. 1–13, 2020, |
[21] | T. Ma, Q. He, G. Cao, and X. Li, “Enhanced Nitrogen Removal from a Recirculating Aquaculture System Using a Calcined FeSx-Packed Denitrification Bioreactor,” ACS Omega, vol. 9, no. 52, pp. 51089–51097, 2024, |
[22] | Y. Xia et al., “Microorganism-mediated denitrogenation of aquaculture systems provoked by poly-β-hydroxybutyrate (PHB),” npj Clean Water, vol. 7, no. 1, pp. 1–11, 2024, |
[23] | S. Niu et al., “Nitrification and denitrification processes in a zero-water exchange aquaculture system: characteristics of the microbial community and potential rates,” Front. Mar. Sci., vol. 10, no. 3, pp. 1–14, 2023, |
[24] | M. Bilal, T. Rasheed, J. E. Sosa‐Hernández, A. Raza, F. Nabeel, and H. M. N. Iqbal, “Biosorption: An interplay between marine algae and potentially toxic elements—A review,” Mar. Drugs, vol. 16, no. 2, pp. 1–16, 2018, |
[25] | C. Filote et al., “Sustainable application of biosorption and bioaccumulation of persistent pollutants in wastewater treatment: Current practice,” Processes, vol. 9, no. 10, pp. 1–39, 2021, |
[26] | S. Mustafa, H. N. Bhatti, M. Maqbool, and M. Iqbal, “Microalgae biosorption, bioaccumulation and biodegradation efficiency for the remediation of wastewater and carbon dioxide mitigation: Prospects, challenges and opportunities,” J. Water Process Eng., vol. 41, no. November 2020, p. 102009, 2021, |
[27] | Y. Hou, R. Jia, P. Ji, B. Li, and J. Zhu, “Organic matter degradation and bacterial communities in surface sediment influenced by Procambarus clarkia,” Front. Microbiol., vol. 13, no. October, pp. 1–16, 2022, |
[28] | S. Ende, J. Henjes, M. Spiller, M. Elshobary, D. Hanelt, and A. Abomohra, “Recent advances in recirculating aquaculture systems and role of microalgae to close system loop,” Bioresour. Technol., vol. 407, no. July, p. 131107, 2024, |
[29] | I. Ambat, S. Bec, E. Peltomaa, V. Srivastava, A. Ojala, and M. Sillanpää, “A synergic approach for nutrient recovery and biodiesel production by the cultivation of microalga species in the fertilizer plant wastewater,” Sci. Rep., vol. 9, no. 1, pp. 1–9, 2019, |
[30] | J. Segura, “Recirculating Aquaculture Systems (RAS): A Solution for Water Efficiency,” Pre QC No. P, vol. 9, 2023, |
[31] | C. Viegas, L. Gouveia, and M. Gonçalves, “Aquaculture wastewater treatment through microalgal. Biomass potential applications on animal feed, agriculture, and energy,” J. Environ. Manage., vol. 286, no. January, 2021, |
[32] | X. Christodoulou et al., “Design and development of pilot photobioreactor for simultaneous microalgae cultivation and aquaculture wastewater treatment,” Process Biochem., vol. 153, no. November 2024, pp. 182–190, 2025, |
[33] | W. Farooq, “Maximizing Energy Content and CO2 Bio-fixation Efficiency of an Indigenous Isolated Microalga Parachlorella kessleri HY-6 Through Nutrient Optimization and Water Recycling During Cultivation,” Front. Bioeng. Biotechnol., vol. 9, no. February, pp. 1–12, 2022, |
[34] | J. Sen Tan et al., “A review on microalgae cultivation and harvesting, and their biomass extraction processing using ionic liquids,” Bioengineered, vol. 11, no. 1, pp. 116–129, 2020, |
[35] | S. Z. Khan, A. A. Zaidi, M. N. Naseer, and H. AlMohamadi, “Nanomaterials for biogas augmentation towards renewable and sustainable energy production: A critical review,” Front. Bioeng. Biotechnol., vol. 10, no. September, pp. 1–29, 2022, |
[36] | M. N. Pervez et al., “A critical review on nanomaterials membrane bioreactor (NMS-MBR) for wastewater treatment,” npj Clean Water, vol. 3, no. 1, 2020, |
[37] | J. M. S. Nanomater and M. Khushnood, “Journal of Materials Science and Nanomaterials Nanomaterials : Surface Area to Volume Ratio,” vol. 8, no. 5, pp. 5–7, 2024, |
[38] | C. Grandclément et al., “From the conventional biological wastewater treatment to hybrid processes, the evaluation of organic micropollutant removal: A review,” Water Res., vol. 111, pp. 297–317, 2017, |
[39] | L. D. Justin and D. O. Olukanni, “Efficiency evaluation of wastewater treatment by three macrophytes using a pilot-constructed wetland system in Ota, Nigeria,” J. Water Health, vol. 22, no. 11, pp. 2040–2053, 2024, |
[40] | J. Jayaraman et al., “Wastewater treatment by algae-based membrane bioreactors: a review of the arrangement of a membrane reactor, physico-chemical properties, advantages and challenges,” RSC Adv., vol. 14, no. 47, pp. 34769–34790, 2024, |
[41] | A. Kouanda and G. Hua, “Determination of nitrate removal kinetics model parameters in woodchip bioreactors,” Water Res., vol. 195, p. 116974, 2021, |
[42] | F. Plauborg, M. H. Skjødt, J. Audet, C. C. Hoffmann, and B. H. Jacobsen, “Cost effectiveness, nitrogen, and phosphorus removal in field-based woodchip bioreactors treating agricultural drainage water,” Environ. Monit. Assess., vol. 195, no. 7, 2023, |
[43] | A. Tsitouras, N. Al-ghussain, J. Butcher, A. Stintzi, and R. Delatolla, “sequencing batch moving bed biofilm reactor treating cheese production wastewater,” vol. 89, no. 12, pp. 1–18. |
[44] | G. Proano-Pena, A. L. Carrano, and D. M. Blersch, “Analysis of very-high surface area 3D-printed media in a moving bed biofilm reactor for wastewater treatment,” PLoS One, vol. 15, no. 8 August, pp. 1–17, 2020, |
[45] | M. E. Rahman et al., “Design, operation and optimization of constructed wetland for removal of pollutant,” Int. J. Environ. Res. Public Health, vol. 17, no. 22, pp. 1–40, 2020, |
[46] | J. Nsanzabaganwa et al., “Impact analysis of constructed wetland for wastewater management in Rwanda,” Front. Environ. Sci., vol. 13, no. April, pp. 1–10, 2025, |
[47] | S. K. Amponsah et al., “Performance of a Horizontal Subsurface Flow Constructed Wetland in Treating Aquaculture Wastewater,” J. Ecol. Eng., vol. 25, no. 10, pp. 53–61, 2024, |
[48] | “Nitrification process in recirculating aquaculture system Mashkhura Babadjanova Zagreb, June, 2017,” 2025. |
[49] | K. Ghyselbrecht et al., “The fate of nitrite and nitrate during anaerobic digestion,” Environ. Technol. (United Kingdom), vol. 40, no. 8, pp. 1013–1026, 2019, |
[50] | A. P. Tom, J. S. Jayakumar, M. Biju, J. Somarajan, and M. A. Ibrahim, “Aquaculture wastewater treatment technologies and their sustainability: A review,” Energy Nexus, vol. 4, no. July, p. 100022, 2021, |
[51] | K. Saas, “The Environmental Benefits of Closed-Loop Aquaculture Systems,” vol. 14, no. 1000798, p. 1000798, 2023, |
[52] | S. B. Kurniawan, A. Ahmad, M. F. Imron, S. R. S. Abdullah, A. R. Othman, and H. A. Hasan, “Achieving a Biocircular Economy in the Aquaculture Sector Through Waste Valorization,” Toxics, vol. 13, no. 2, pp. 1–27, 2025, |
[53] | A. Shitu et al., “Recent advances in application of moving bed bioreactors for wastewater treatment from recirculating aquaculture systems: A review,” Aquac. Fish., vol. 7, no. 3, pp. 244–258, 2022, |
[54] | R. Wang, Z. Pan, Y. Chen, Z. Tan, and J. Zhang, “Influent quality and quantity prediction in wastewater treatment plant: Model construction and evaluation,” Polish J. Environ. Stud., vol. 30, no. 5, pp. 4267–4276, 2021, |
[55] | F. Kaiser and H. Harbach, “InnoFish-innovative adaptation of integrated aquaculture in an established extensive fish farm,” AACL Bioflux, vol. 15, no. 2, pp. 873–877, 2022. |
[56] | B. Geng, Y. Li, X. Liu, J. Ye, and W. Guo, “Effective treatment of aquaculture wastewater with mussel/microalgae/bacteria complex ecosystem: a pilot study,” Sci. Rep., vol. 12, no. 1, pp. 1–12, 2022, |
[57] | J. Wu, S. W. Rogers, R. Schaummann, C. Higgins, and N. Price, “Bioextractive aquaculture as an alternative nutrient management strategy for water resource recovery facilities,” Water Res., vol. 212, no. May 2021, p. 118092, 2022, |
APA Style
Molla, A. (2025). Advances in Bioreactor Technologies for Sustainable Aquaculture Water Treatment. American Journal of Bioscience and Bioengineering, 13(5), 92-98. https://doi.org/10.11648/j.bio.20251305.11
ACS Style
Molla, A. Advances in Bioreactor Technologies for Sustainable Aquaculture Water Treatment. Am. J. BioSci. Bioeng. 2025, 13(5), 92-98. doi: 10.11648/j.bio.20251305.11
@article{10.11648/j.bio.20251305.11, author = {Alebachew Molla}, title = {Advances in Bioreactor Technologies for Sustainable Aquaculture Water Treatment}, journal = {American Journal of Bioscience and Bioengineering}, volume = {13}, number = {5}, pages = {92-98}, doi = {10.11648/j.bio.20251305.11}, url = {https://doi.org/10.11648/j.bio.20251305.11}, eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20251305.11}, abstract = {Advances in bioreactor technologies are transforming sustainable aquaculture water treatment by improving pollutant removal and supporting environmental conservation and resource recovery. Recirculating Aquaculture Systems (RAS) represent a leading sustainable approach by integrating physical, chemical, and biological treatment processes to recycle water within the system, minimizing freshwater consumption and effluent discharge. Innovative biological systems integrated with Recirculating Aquaculture Systems (RAS) including Moving Bed Biofilm Reactors, membrane bioreactors, anaerobic digesters, photobioreactors, and biofloc efficiently reduce nitrogen, phosphorus, organic matter, and other pollutants using diverse microbial communities without harmful chemicals. Recent developments feature microalgae cultivation for carbon capture and nutrient recycling, nanotechnology to boost microbial performance, and hybrid treatment methods for enhanced effectiveness. While Moving Bed Biofilm Reactors offer high ammonia and organic removal in compact setups, anaerobic bioreactors provide cost-effective nitrate reduction, and constructed wetlands effectively remove organics and phosphorus with more space needs. These bioreactors technology enhance aquaculture sustainability by reducing pollutant loads, mitigating eutrophication risks, and improving fish health through optimized water quality. Despite operational and cost challenges, these technologies promote water reuse, lower pollutant discharge, and enable circular economy practices like bioenergy production. Future research focuses on tailored, integrated treatments, engineered microbes, and resource-loop closing frameworks to bolster sustainability, regulatory compliance, and economic viability in intensive aquaculture. The aim of this review article is to examine recent innovations and developments in bioreactor technologies applied to aquaculture wastewater treatment.}, year = {2025} }
TY - JOUR T1 - Advances in Bioreactor Technologies for Sustainable Aquaculture Water Treatment AU - Alebachew Molla Y1 - 2025/10/09 PY - 2025 N1 - https://doi.org/10.11648/j.bio.20251305.11 DO - 10.11648/j.bio.20251305.11 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 92 EP - 98 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20251305.11 AB - Advances in bioreactor technologies are transforming sustainable aquaculture water treatment by improving pollutant removal and supporting environmental conservation and resource recovery. Recirculating Aquaculture Systems (RAS) represent a leading sustainable approach by integrating physical, chemical, and biological treatment processes to recycle water within the system, minimizing freshwater consumption and effluent discharge. Innovative biological systems integrated with Recirculating Aquaculture Systems (RAS) including Moving Bed Biofilm Reactors, membrane bioreactors, anaerobic digesters, photobioreactors, and biofloc efficiently reduce nitrogen, phosphorus, organic matter, and other pollutants using diverse microbial communities without harmful chemicals. Recent developments feature microalgae cultivation for carbon capture and nutrient recycling, nanotechnology to boost microbial performance, and hybrid treatment methods for enhanced effectiveness. While Moving Bed Biofilm Reactors offer high ammonia and organic removal in compact setups, anaerobic bioreactors provide cost-effective nitrate reduction, and constructed wetlands effectively remove organics and phosphorus with more space needs. These bioreactors technology enhance aquaculture sustainability by reducing pollutant loads, mitigating eutrophication risks, and improving fish health through optimized water quality. Despite operational and cost challenges, these technologies promote water reuse, lower pollutant discharge, and enable circular economy practices like bioenergy production. Future research focuses on tailored, integrated treatments, engineered microbes, and resource-loop closing frameworks to bolster sustainability, regulatory compliance, and economic viability in intensive aquaculture. The aim of this review article is to examine recent innovations and developments in bioreactor technologies applied to aquaculture wastewater treatment. VL - 13 IS - 5 ER -