Antimicrobial Activity of <i>Aegle marmelos </i>L. Leaf Extract

Shahanaz Khatun; Nasrin Ferdous

doi:doi:10.11648/j.bio.20231105.13

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Antimicrobial Activity of Aegle marmelos L. Leaf Extract

Shahanaz Khatun, Nasrin Ferdous

Published in American Journal of Bioscience and Bioengineering (Volume 11, Issue 5)

Received: 20 June 2023 Accepted: 13 July 2023 Published: 27 September 2023

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Abstract

Ethno medicine has been gaining popularity for years, yet there is still a vast amount of medicinal flora that remains undiscovered through research. The in vitro antimicrobial activity of methanol and water extract from the leaf of medicinal plant Aegle marmelos L. were investigated. The investigation was performed against three Gram positive bacteria (Bacillus megaterium, Bacillus subtilis, and Staphylococcus pneumonia) and five Gram negative (Salmonella typhi, Vibrio parahaemolyticus, Escherichia coli, Shigella dysenteriae and Shigella sonnei) bacteria and eight fungi (T. mentagrophytes, M. canis, T. rubrum, E. floccosum, Penicilium sp, Fusarium species, Aspergillus niger and Mucor) by disc diffusion assay method. The lowest concentration of every extract considered as the minimal inhibitory concentration (MIC) values were determined for both test organisms. Statistical significance was determined with one-way ANOVA and the level of significance was P < 0.05. The water extract of leaves at the concentration of 600 μg/ disc showed the higher activity against S. typhi (13±0.1), and S. dysenteriae (15±0.2) than methanol extract. Methanol extract showed the higher activity against E. coli (18±0.1), V. parahaemolyticus (19±0.2) and S. pneumonia (15±0.3) than water extract. Methanol extract of A. marmelos leaf showed more susceptibility towards skin disease causing fungi like T. mentagrophytes, M. canis, T. rubrum, E. floccosum, than the non-skin disease causing fungi like Penicilium sp, Fusarium species and Mucor. The result implies that the both methanol and water extract of Aegle marmelos, L. has great potential for medicinal purposes due to its antimicrobial quality.

Published in	American Journal of Bioscience and Bioengineering (Volume 11, Issue 5)
DOI	10.11648/j.bio.20231105.13
Page(s)	75-78
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Antimicrobial Activity, Medicinal Plant, Aegle marmelos, Bacteria, Fungi

References

[1]	PANKKAJALAKSHMI, V. V & VENUGOPAL, T. V. (1994) Antidermatophytic activity of neem (Azadiracta indica) leaves in vitro, Indian Journal of Pharmacology, 26: 141-143.
[2]	VADAPUDI, V. & CHANDRASHEKAR, N. K. (2010) In Vitro bioactivity of Indian Medicinal Plant Calotropis Procera (Ait.), Journal of Global Pharma Technology. 2 (2): 43-45.
[3]	ALOM, M. A., HABIB, M. R., NIKKON, F., RAHMAN, M & KARIM, M. R. (2008) Antimicrobial activity of akanda (Calotropis gigantean L.) on some pathogenicbacteria, Bangladesh Journal of Scientific and Industrial Research, 43 (3): 397-404.
[4]	FARRUKH, U., SHAREEF, H., MAHMUD, S., ALI, S. A. and RIZWANI, G. H. (2008) Antimicrobial activities of Coccinia grandis L., Pakistan Journal of Botani, 40 (3): 1259-1262.
[5]	RAHMAN, M. M., SEIKH, M. M. I., SHARMIN, S. A., ISLAM, M. S., RAHMAN, M. A., RAHMAN, M. M. & ALAM, M. F. (2009) Antimicrobial activity of leaf juice and extracts of Moringa oleifera Lam. Against some human pathogenic bacteria. CMU. Journal of Natural Science, 8 (2): 219-227.
[6]	HO, C. H., NORYATI, I., SULAIMAN, S. F. and ROSMA, A. (2010) In vitro antibacterial and antioxidant activities of Orthosiphon stamineus Benth. extracts against food-borne bacteria, Food Chemistry, 122: 1168-1172.
[7]	DALAHAYA, C., RAINFORD, L., NIHOLSON, A., MITCHEL, S., LINDO, J & AHMAD, M. (2009) Antibacterial and antifungal analysis of crude extracts from the leaves of Callistemon viminalis, Journal of Medical and Biological sciences, 3 (1): 1-7.
[8]	KRITZINGER, Q., LAIL, N. & AVELING, T. A. S. (2005) Antimicrobial activity of cowpea (Vigna unguiculata) leaf extracts, South African Journal of botany, 71 (1): 45-48.
[9]	DEWANJEE, S., KUNDU, M., MAITI, A., MAJUMDAR, R., MAJUMDAR, A. & MANDAL, S. C. (2007) In Vitro evaluation of antimicrobial activity of crude extract from plants Diospyros peregrina, Coccinia grandis and Swietenia macrophylla. Tropical Journal of Pharmaceuticcal Research, 6: 773-778.
[10]	HOOKER, J. D. (1975) The flora of British India, Reeve, United Kingdom, Vol. 1, pp 516–517.
[11]	ISLAM, R., HOSSAIN, M., KARIM, M. R. & JOARDER, O. I. (1995) Regeneration of Aegle marmelos (L.) Corr., plantlets in vitro from callus cultures of embryonic tissues, Current Science, 69: 494–5.
[12]	CHOPRA, R. (1982) Indigenous drugs of India Calcutta. Academic publishers, pp 71-76.
[13]	ARUMUGAMA, S., KAVIMANIB, S., KADALMANIC, B., AHMEED, A. B. A., AKBARSHA, M. A. & RAO, M. V. (2008) Antidiabetic activity of leaf and callus extracts of Aegle marmelos in rabbit, Science Asia, 34: 317-321.
[14]	JAGETIA, G. C., VENKATESH, P. & BALLIGA, M. S. (2005) Aegle marmelos (L) Correa inhibits the proliferation of transplanted Ehrlich ascites carcinoma in mice, Biological and Pharmaceutical Bulletin, 28: 58-63.
[15]	ARUL, V., MIYAZAKI, S. & DHANANJAYAN, R. (2005) Studies on the antiinflammatory, antipyretic and analgesic properties of the leaves of Aegle marmelos Correa., Journal of Ethnopharmacology, 96: 159-163.
[16]	MISRA, P., PAL, N. L., GURU, P. Y., KATIYAR, J. C. & TANDON, J. S. (1991) Antimalarial activity of traditional plant against erythrocyte stages of Plasmodium berghei International Journal of Pharmacology. 19: 29-34.
[17]	RAJADURAI, M. & PRINCE, P. S. (2005) Comparative effects of Aegle marmelos extract and alpha-tocopherol on serum lipids, lipid peroxides and cardiac enzyme levels in rats with isoproterenol-induced myocardial infarction, Singapore Medical Journal, 46: 78-82.
[18]	RANA, B. K., SINGH, U. P. & TANEJA, A. (1997) Antifungal activity and kinetics of inhibition by essential oil isolated from leaves of Aegle marmelos, Journal of Ethnopharmacology, 57: 29-34.
[19]	VEERAPPAN, A. K. S. & RENGANATHAN, D. (2000) Cardiotonic effect of Aegle marmelos Corr. On amphibian heart in situ preparation, Proc. 6th Internet World Congress for Biomed. Sci.
[20]	PRAKASH, C. G., DUTTA, B., PANT, D., JOSHI, P. & LOHAR, D. R. (2009) In vitro antibacterial activity of Artemisia annua Linn, Growing in India, International journal of Green Pharmacy, 4: 255-258.
[21]	VANDER, B. D. A. & VLIETNCK, B. (1991) Screening methods of antibacterial and antiviral agents from higher plants. IN: Assay for Bioactivity (K. Hostiettman Academic Press London), pp 47-69.
[22]	ASHRADUZZAMAN, M., MOHAMMAD, A. A., KHATUN, S. & ABSAR, N. (2016) Antimicribial activity of V. unguiculata L. Walp seed oil, International journal of biotechnology for wellness industries. 5: 70-75.
[23]	SIVARAJ, R., BALAKRISHNAN, A., THENMOHI, M. & VENCKATESH, R. (2011) Antimicrobial activity of Aegle marmelos, Ruta graveolens, Opuntia dellini, Euphorbia royleana and Euphorbia antiquorum, Journal of Pharmacy Research, 4 (5): 1507-1508.
[24]	KHATUN, S., KHAN, MMH., ASHRADUZZAMAN, M., PERVIN, F., BARI, L. & ABSAR, N. (2009) Antibacterial activity and cytotoxicity of three lectins purified from Moringa oleifera Lam. Leaves, Journal of Biosciences, 17: 89-94.
[25]	PERUMALSAMY, R., IGNACHIMUTHU, S. & SEN, A. (1988) Screening of 34 Indian Medicinal plants for antimicrobial properities, Journal of Ethnopharmacology, 62: 173-81.
[26]	AHMAD, I., MEHMOOD, Z., MOHAMMAD, P. & AHMED, S. (2000) Antimicrobial potency and synergistic activity of five traditionally used Indian medicinal plants, Journal of Medicinal Aromatic Plant Science, 22: 173-176.
[27]	RANI, S. A. & MURTY, S. U. (2006) Antifungal potential of flower head extract of Spilanthes acmella Linn., African Journal of Biomedical Research, 9: 67-69.
[28]	KHATUN, S., PERVIN, F., KARIM, M. R., ASHRADUZZAMAN, M. & ROSMA, A. (2012) Phytochemical screening and antimicrobial activity of Coccinia cordifolia L. plant Pakistan Journal of Pharmaceutical Sciences, 25 (4): 757-761.

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APA Style

Shahanaz Khatun, Nasrin Ferdous. (2023). Antimicrobial Activity of Aegle marmelos L. Leaf Extract. American Journal of Bioscience and Bioengineering, 11(5), 75-78. https://doi.org/10.11648/j.bio.20231105.13

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ACS Style

Shahanaz Khatun; Nasrin Ferdous. Antimicrobial Activity of Aegle marmelos L. Leaf Extract. Am. J. BioSci. Bioeng. 2023, 11(5), 75-78. doi: 10.11648/j.bio.20231105.13

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AMA Style

Shahanaz Khatun, Nasrin Ferdous. Antimicrobial Activity of Aegle marmelos L. Leaf Extract. Am J BioSci Bioeng. 2023;11(5):75-78. doi: 10.11648/j.bio.20231105.13

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@article{10.11648/j.bio.20231105.13,
  author = {Shahanaz Khatun and Nasrin Ferdous},
  title = {Antimicrobial Activity of Aegle marmelos L. Leaf Extract},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {11},
  number = {5},
  pages = {75-78},
  doi = {10.11648/j.bio.20231105.13},
  url = {https://doi.org/10.11648/j.bio.20231105.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20231105.13},
  abstract = {Ethno medicine has been gaining popularity for years, yet there is still a vast amount of medicinal flora that remains undiscovered through research. The in vitro antimicrobial activity of methanol and water extract from the leaf of medicinal plant Aegle marmelos L. were investigated. The investigation was performed against three Gram positive bacteria (Bacillus megaterium, Bacillus subtilis, and Staphylococcus pneumonia) and five Gram negative (Salmonella typhi, Vibrio parahaemolyticus, Escherichia coli, Shigella dysenteriae and Shigella sonnei) bacteria and eight fungi (T. mentagrophytes, M. canis, T. rubrum, E. floccosum, Penicilium sp, Fusarium species, Aspergillus niger and Mucor) by disc diffusion assay method. The lowest concentration of every extract considered as the minimal inhibitory concentration (MIC) values were determined for both test organisms. Statistical significance was determined with one-way ANOVA and the level of significance was P  S. typhi (13±0.1), and S. dysenteriae (15±0.2) than methanol extract. Methanol extract showed the higher activity against E. coli (18±0.1), V. parahaemolyticus (19±0.2) and S. pneumonia (15±0.3) than water extract. Methanol extract of A. marmelos leaf showed more susceptibility towards skin disease causing fungi like T. mentagrophytes, M. canis, T. rubrum, E. floccosum, than the non-skin disease causing fungi like Penicilium sp, Fusarium species and Mucor. The result implies that the both methanol and water extract of Aegle marmelos, L. has great potential for medicinal purposes due to its antimicrobial quality.},
 year = {2023}
}

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TY - JOUR
T1 - Antimicrobial Activity of Aegle marmelos L. Leaf Extract
AU - Shahanaz Khatun
AU - Nasrin Ferdous
Y1 - 2023/09/27
PY - 2023
N1 - https://doi.org/10.11648/j.bio.20231105.13
DO - 10.11648/j.bio.20231105.13
T2 - American Journal of Bioscience and Bioengineering
JF - American Journal of Bioscience and Bioengineering
JO - American Journal of Bioscience and Bioengineering
SP - 75
EP - 78
PB - Science Publishing Group
SN - 2328-5893
UR - https://doi.org/10.11648/j.bio.20231105.13
AB - Ethno medicine has been gaining popularity for years, yet there is still a vast amount of medicinal flora that remains undiscovered through research. The in vitro antimicrobial activity of methanol and water extract from the leaf of medicinal plant Aegle marmelos L. were investigated. The investigation was performed against three Gram positive bacteria (Bacillus megaterium, Bacillus subtilis, and Staphylococcus pneumonia) and five Gram negative (Salmonella typhi, Vibrio parahaemolyticus, Escherichia coli, Shigella dysenteriae and Shigella sonnei) bacteria and eight fungi (T. mentagrophytes, M. canis, T. rubrum, E. floccosum, Penicilium sp, Fusarium species, Aspergillus niger and Mucor) by disc diffusion assay method. The lowest concentration of every extract considered as the minimal inhibitory concentration (MIC) values were determined for both test organisms. Statistical significance was determined with one-way ANOVA and the level of significance was P S. typhi (13±0.1), and S. dysenteriae (15±0.2) than methanol extract. Methanol extract showed the higher activity against E. coli (18±0.1), V. parahaemolyticus (19±0.2) and S. pneumonia (15±0.3) than water extract. Methanol extract of A. marmelos leaf showed more susceptibility towards skin disease causing fungi like T. mentagrophytes, M. canis, T. rubrum, E. floccosum, than the non-skin disease causing fungi like Penicilium sp, Fusarium species and Mucor. The result implies that the both methanol and water extract of Aegle marmelos, L. has great potential for medicinal purposes due to its antimicrobial quality.
VL - 11
IS - 5
ER -

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Author Information

Shahanaz Khatun

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh
Nasrin Ferdous

Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

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APA Style

Shahanaz Khatun, Nasrin Ferdous. (2023). Antimicrobial Activity of Aegle marmelos L. Leaf Extract. American Journal of Bioscience and Bioengineering, 11(5), 75-78. https://doi.org/10.11648/j.bio.20231105.13

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ACS Style

Shahanaz Khatun; Nasrin Ferdous. Antimicrobial Activity of Aegle marmelos L. Leaf Extract. Am. J. BioSci. Bioeng. 2023, 11(5), 75-78. doi: 10.11648/j.bio.20231105.13

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AMA Style

Shahanaz Khatun, Nasrin Ferdous. Antimicrobial Activity of Aegle marmelos L. Leaf Extract. Am J BioSci Bioeng. 2023;11(5):75-78. doi: 10.11648/j.bio.20231105.13

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@article{10.11648/j.bio.20231105.13,
  author = {Shahanaz Khatun and Nasrin Ferdous},
  title = {Antimicrobial Activity of Aegle marmelos L. Leaf Extract},
  journal = {American Journal of Bioscience and Bioengineering},
  volume = {11},
  number = {5},
  pages = {75-78},
  doi = {10.11648/j.bio.20231105.13},
  url = {https://doi.org/10.11648/j.bio.20231105.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20231105.13},
  abstract = {Ethno medicine has been gaining popularity for years, yet there is still a vast amount of medicinal flora that remains undiscovered through research. The in vitro antimicrobial activity of methanol and water extract from the leaf of medicinal plant Aegle marmelos L. were investigated. The investigation was performed against three Gram positive bacteria (Bacillus megaterium, Bacillus subtilis, and Staphylococcus pneumonia) and five Gram negative (Salmonella typhi, Vibrio parahaemolyticus, Escherichia coli, Shigella dysenteriae and Shigella sonnei) bacteria and eight fungi (T. mentagrophytes, M. canis, T. rubrum, E. floccosum, Penicilium sp, Fusarium species, Aspergillus niger and Mucor) by disc diffusion assay method. The lowest concentration of every extract considered as the minimal inhibitory concentration (MIC) values were determined for both test organisms. Statistical significance was determined with one-way ANOVA and the level of significance was P  S. typhi (13±0.1), and S. dysenteriae (15±0.2) than methanol extract. Methanol extract showed the higher activity against E. coli (18±0.1), V. parahaemolyticus (19±0.2) and S. pneumonia (15±0.3) than water extract. Methanol extract of A. marmelos leaf showed more susceptibility towards skin disease causing fungi like T. mentagrophytes, M. canis, T. rubrum, E. floccosum, than the non-skin disease causing fungi like Penicilium sp, Fusarium species and Mucor. The result implies that the both methanol and water extract of Aegle marmelos, L. has great potential for medicinal purposes due to its antimicrobial quality.},
 year = {2023}
}

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