Isolation trials from pepper leaves showing bacterial spot collected from Giza, Kalubia and Behera governorates yielded 13,12,9,6 isolates of Bacillus subtilis, Pseudomonas spp., Xanthomonas campestris pv. vesicatoria and P. fluorescens, respectively. The isolated strains of X.c. pv. vesicatoria were yellow Gram negative of short rods and produced Xanthomonadins and the other identification criteria proved that these isolates are X.c. pv. vesicatoria. Pathogenicity test of these isolates on Balady pepper cv. indicated that all of them were pathogenic and isolate No.3 of Kalubia governorate resulted in the highest infection. P. putida was most efficient bioagent in inhibiting of the pathogenic bacterium followed by P. fluorescens, while B. subtilis was the lowest efficient one. The fungicide Efdal Bakirox was the most efficient one in inhibiting growth of the causal bacterium on the medium more than the fungicides Roxil and Tango. The fungicide Tango and Roxil failed to cause any inhibition to the causal bacterium at 100 ppm. Field experiments during 2014 and 2015 growing seasons revealed that spraying pepper plants with the tested bioagents and the fungicides four times resulted in significant reduction to the severity of the natural infection by the causal bacteriaum with significant increase to the produced fruit pods compared with the control. The tested fungicides were more efficient in this regard than the bioagents. Roxil was the most efficient treatment in reducing the severity of the disease and increasing the produced pod yield. Meanwhile, the bioagent B. subtilis was the lowest efficient treatment and the other treatments recorded intermediate figures. Total phenols and vitamin-c content as well as the activity of chitinase, peroxidase and polyphenoloxidase in pepper leaves infected by X.c. pv. vesicatoria were greatly lowered in the infected leaves compared with the uninfected leaves and the tested bioagents and fungicides resulted in considerable increase to these chemicals compared with the infected leaves.
| Published in | American Journal of Bioscience and Bioengineering (Volume 5, Issue 1) |
| DOI | 10.11648/j.bio.20170501.17 |
| Page(s) | 41-49 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Pepper, Bioagents, Fungicides, Enzymes, Management, Phenols, Vitamin-C and Xanthomonas campestris pv. vesicatoria
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APA Style
Eman O. Hassan, Marwa A. Zyton. (2017). Management of Bacterial Spot of Pepper Caused by Xanthomonas campestris pv. vesicatoria. American Journal of Bioscience and Bioengineering, 5(1), 41-49. https://doi.org/10.11648/j.bio.20170501.17
ACS Style
Eman O. Hassan; Marwa A. Zyton. Management of Bacterial Spot of Pepper Caused by Xanthomonas campestris pv. vesicatoria. Am. J. BioSci. Bioeng. 2017, 5(1), 41-49. doi: 10.11648/j.bio.20170501.17
AMA Style
Eman O. Hassan, Marwa A. Zyton. Management of Bacterial Spot of Pepper Caused by Xanthomonas campestris pv. vesicatoria. Am J BioSci Bioeng. 2017;5(1):41-49. doi: 10.11648/j.bio.20170501.17
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Download@article{10.11648/j.bio.20170501.17,
author = {Eman O. Hassan and Marwa A. Zyton},
title = {Management of Bacterial Spot of Pepper Caused by Xanthomonas campestris pv. vesicatoria},
journal = {American Journal of Bioscience and Bioengineering},
volume = {5},
number = {1},
pages = {41-49},
doi = {10.11648/j.bio.20170501.17},
url = {https://doi.org/10.11648/j.bio.20170501.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20170501.17},
abstract = {Isolation trials from pepper leaves showing bacterial spot collected from Giza, Kalubia and Behera governorates yielded 13,12,9,6 isolates of Bacillus subtilis, Pseudomonas spp., Xanthomonas campestris pv. vesicatoria and P. fluorescens, respectively. The isolated strains of X.c. pv. vesicatoria were yellow Gram negative of short rods and produced Xanthomonadins and the other identification criteria proved that these isolates are X.c. pv. vesicatoria. Pathogenicity test of these isolates on Balady pepper cv. indicated that all of them were pathogenic and isolate No.3 of Kalubia governorate resulted in the highest infection. P. putida was most efficient bioagent in inhibiting of the pathogenic bacterium followed by P. fluorescens, while B. subtilis was the lowest efficient one. The fungicide Efdal Bakirox was the most efficient one in inhibiting growth of the causal bacterium on the medium more than the fungicides Roxil and Tango. The fungicide Tango and Roxil failed to cause any inhibition to the causal bacterium at 100 ppm. Field experiments during 2014 and 2015 growing seasons revealed that spraying pepper plants with the tested bioagents and the fungicides four times resulted in significant reduction to the severity of the natural infection by the causal bacteriaum with significant increase to the produced fruit pods compared with the control. The tested fungicides were more efficient in this regard than the bioagents. Roxil was the most efficient treatment in reducing the severity of the disease and increasing the produced pod yield. Meanwhile, the bioagent B. subtilis was the lowest efficient treatment and the other treatments recorded intermediate figures. Total phenols and vitamin-c content as well as the activity of chitinase, peroxidase and polyphenoloxidase in pepper leaves infected by X.c. pv. vesicatoria were greatly lowered in the infected leaves compared with the uninfected leaves and the tested bioagents and fungicides resulted in considerable increase to these chemicals compared with the infected leaves.},
year = {2017}
}
TY - JOUR T1 - Management of Bacterial Spot of Pepper Caused by Xanthomonas campestris pv. vesicatoria AU - Eman O. Hassan AU - Marwa A. Zyton Y1 - 2017/02/23 PY - 2017 N1 - https://doi.org/10.11648/j.bio.20170501.17 DO - 10.11648/j.bio.20170501.17 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 41 EP - 49 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20170501.17 AB - Isolation trials from pepper leaves showing bacterial spot collected from Giza, Kalubia and Behera governorates yielded 13,12,9,6 isolates of Bacillus subtilis, Pseudomonas spp., Xanthomonas campestris pv. vesicatoria and P. fluorescens, respectively. The isolated strains of X.c. pv. vesicatoria were yellow Gram negative of short rods and produced Xanthomonadins and the other identification criteria proved that these isolates are X.c. pv. vesicatoria. Pathogenicity test of these isolates on Balady pepper cv. indicated that all of them were pathogenic and isolate No.3 of Kalubia governorate resulted in the highest infection. P. putida was most efficient bioagent in inhibiting of the pathogenic bacterium followed by P. fluorescens, while B. subtilis was the lowest efficient one. The fungicide Efdal Bakirox was the most efficient one in inhibiting growth of the causal bacterium on the medium more than the fungicides Roxil and Tango. The fungicide Tango and Roxil failed to cause any inhibition to the causal bacterium at 100 ppm. Field experiments during 2014 and 2015 growing seasons revealed that spraying pepper plants with the tested bioagents and the fungicides four times resulted in significant reduction to the severity of the natural infection by the causal bacteriaum with significant increase to the produced fruit pods compared with the control. The tested fungicides were more efficient in this regard than the bioagents. Roxil was the most efficient treatment in reducing the severity of the disease and increasing the produced pod yield. Meanwhile, the bioagent B. subtilis was the lowest efficient treatment and the other treatments recorded intermediate figures. Total phenols and vitamin-c content as well as the activity of chitinase, peroxidase and polyphenoloxidase in pepper leaves infected by X.c. pv. vesicatoria were greatly lowered in the infected leaves compared with the uninfected leaves and the tested bioagents and fungicides resulted in considerable increase to these chemicals compared with the infected leaves. VL - 5 IS - 1 ER -
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