Food grade proteases are proteyolytic enzymes having application in baking, food processing, protein modification etc. As a commodity product, pressure on protease market is on prize reduction and increasing performance. Hence our objective was to isolate a potent protease-producing microorganism and formulate a cost effective medium for neutral protease synthesis by the potent microbial culture. In order to achieve the objective, a proteolytic bacterium was isolated from soil using milk agar medium and the bacteria was identified as Bacillus sp. by morphological and biochemical characterization. Dairy industry effluent was then studied as a medium for neutral protease synthesis by the potent bacteria. Supplementation of mineral salt to the medium did not show profound influence of environmental factors such as medium pH, incubation temperature, agitation rate and incubation time on enzyme production. Optimum enzyme titers were found at pH7 when incubated at 37°C and 120 rpm 48 h. Dairy industry effluent was thus found to be a cost effective medium for neutral protease synthesis by Bacillus sp.
| Published in | American Journal of Bioscience and Bioengineering (Volume 4, Issue 6) |
| DOI | 10.11648/j.bio.20160406.15 |
| Page(s) | 90-95 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Dairy Industry, Dairy Effluent, Bacillus sp., Neutral Protease, Milk Agar, Mineral Salt Medium
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APA Style
P. C. Madhu. (2017). Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp.. American Journal of Bioscience and Bioengineering, 4(6), 90-95. https://doi.org/10.11648/j.bio.20160406.15
ACS Style
P. C. Madhu. Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp.. Am. J. BioSci. Bioeng. 2017, 4(6), 90-95. doi: 10.11648/j.bio.20160406.15
AMA Style
P. C. Madhu. Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp.. Am J BioSci Bioeng. 2017;4(6):90-95. doi: 10.11648/j.bio.20160406.15
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Download@article{10.11648/j.bio.20160406.15,
author = {P. C. Madhu},
title = {Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp.},
journal = {American Journal of Bioscience and Bioengineering},
volume = {4},
number = {6},
pages = {90-95},
doi = {10.11648/j.bio.20160406.15},
url = {https://doi.org/10.11648/j.bio.20160406.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bio.20160406.15},
abstract = {Food grade proteases are proteyolytic enzymes having application in baking, food processing, protein modification etc. As a commodity product, pressure on protease market is on prize reduction and increasing performance. Hence our objective was to isolate a potent protease-producing microorganism and formulate a cost effective medium for neutral protease synthesis by the potent microbial culture. In order to achieve the objective, a proteolytic bacterium was isolated from soil using milk agar medium and the bacteria was identified as Bacillus sp. by morphological and biochemical characterization. Dairy industry effluent was then studied as a medium for neutral protease synthesis by the potent bacteria. Supplementation of mineral salt to the medium did not show profound influence of environmental factors such as medium pH, incubation temperature, agitation rate and incubation time on enzyme production. Optimum enzyme titers were found at pH7 when incubated at 37°C and 120 rpm 48 h. Dairy industry effluent was thus found to be a cost effective medium for neutral protease synthesis by Bacillus sp.},
year = {2017}
}
TY - JOUR T1 - Utilization of Dairy Effluent for Food Grade Protease Production Using Bacillus sp. AU - P. C. Madhu Y1 - 2017/01/24 PY - 2017 N1 - https://doi.org/10.11648/j.bio.20160406.15 DO - 10.11648/j.bio.20160406.15 T2 - American Journal of Bioscience and Bioengineering JF - American Journal of Bioscience and Bioengineering JO - American Journal of Bioscience and Bioengineering SP - 90 EP - 95 PB - Science Publishing Group SN - 2328-5893 UR - https://doi.org/10.11648/j.bio.20160406.15 AB - Food grade proteases are proteyolytic enzymes having application in baking, food processing, protein modification etc. As a commodity product, pressure on protease market is on prize reduction and increasing performance. Hence our objective was to isolate a potent protease-producing microorganism and formulate a cost effective medium for neutral protease synthesis by the potent microbial culture. In order to achieve the objective, a proteolytic bacterium was isolated from soil using milk agar medium and the bacteria was identified as Bacillus sp. by morphological and biochemical characterization. Dairy industry effluent was then studied as a medium for neutral protease synthesis by the potent bacteria. Supplementation of mineral salt to the medium did not show profound influence of environmental factors such as medium pH, incubation temperature, agitation rate and incubation time on enzyme production. Optimum enzyme titers were found at pH7 when incubated at 37°C and 120 rpm 48 h. Dairy industry effluent was thus found to be a cost effective medium for neutral protease synthesis by Bacillus sp. VL - 4 IS - 6 ER -
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