Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins

Verônica Lisboa Santos; Juliana Bueno da Silva; Franciane Cristina de Figueiredo; Carlos Paulo Henrique Ronchi; Edenilse Gopinger

doi:doi:10.11648/j.avs.20221004.11

Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger

Published in Animal and Veterinary Sciences (Volume 10, Issue 4)

Received: 27 June 2022 Accepted: 14 July 2022 Published: 26 July 2022

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.

Published in	Animal and Veterinary Sciences (Volume 10, Issue 4)
DOI	10.11648/j.avs.20221004.11
Page(s)	78-82
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Beta-Glucans, Immunity, Interleukin, Silymarin, Organic Selenium

References

[1]	Aly, E., Madbouly, Y. (2016). The Effect of Mycotoxins on Immune Response of Broilers to the Live ND Vaccines Applied by Different Routes. Middle East Journal of Applied Sciences, 06 (01), 51-58.
[2]	Arseniy E. Y., Anton G. K. (2014). Interleukins in Cancer Biology: Their Heterogeneous Role. Elsevier; London, UK: Academic Press; Cambridge, MA, USA.
[3]	Bello R. O., Chin V. K., Abd Rachman Isnadi MF et al. (2018). The Role, Involvement and Function(s) of Interleukin-35 and Interleukin-37 in Disease Pathogenesis. International Journal of Molecular Sciences. 19 (4).
[4]	Bijak, M. (2017). Silybin, a Major Bioactive Component of Milk Thistle (Silybum marianum L. Gaernt.)—Chemistry, Bioavailability, and Metabolism. Molecules, 22, 1-11.
[5]	Borzecki, P., Borzecka, A., Chylinska-Wrzos, P., Lis-Sochocka, M., Wawryk-Gawda, E., Jodlowska-Jedrych. (2019). Evaluation of interleukin-6 concentration in the liver of Albino Swiss mice after intoxication with various doses of patulin. Curr. Issues Pharm. Med. Sci., 32 (1), 34-39.
[6]	Cho, M. J., Ellebrecht, C. T., Payne, A. S. (2015). The dual nature of interleukin-10 in pemphigus vulgaris. Cytokine, 73 (2), 335-341.
[7]	De Smedt, T., van Mechelen, M., De Becker, G., Urbain, J., Leo, O. and Moser, M. (1997). Effect of interleukin-10 on dendritic cell maturation and function. European Journal of Immunology. 27, 1229-1235.
[8]	Duntas, L. H. (2012). The evolving role of selenium in the treatment of Graves’disease and ophthalmopathy. J Thyroid Res, 736161.
[9]	Duque A. G., & Descoteaux A. (2014). Macrophage Cytokines: Involvement in Immunity and Infectious Diseases. Front. Immunol, 5, 491.
[10]	Esmaeila, N., Anarakib, B. S., Gharagozlooc, M., Moayedia, B. (2017). Silymarin impacts on immune system as an immunomodulator: One key for many locks. International Immunopharmacology, 50, 194-201.
[11]	Hofstetter, U. (2007). The negative effects of various mycotoxins on the immune system of poultry. BIOMIN Newsletter, 5-46.
[12]	Holanda, D. M., Kim, S. W. (2020). Efficacy of Mycotoxin Detoxifiers on Health and Growth of Newly-Weaned Pigs under Chronic Dietary Challenge of Deoxynivalenol. Toxins, 12, 311.
[13]	Iyer, S. S., Cheng, G. (2012). Role of Interleukin 10 Transcriptional Regulation in Inflammation and Autoimmune Disease. Crit Rev Immunol. 32 (1), 23-63.
[14]	Khan, A. Z., Khan, I. U., Khan, S. …Ran L. (2019). Selenium-enriched probiotics improve hepatic protection by regulating pro-inflammatory cytokines and antioxidant capacity in broilers under heat stress conditions Journal of Advanced Veterinary and Animal Research. 6 (3), 355–361.
[15]	Kim, S. H., Oh, D. S., Oh, J. Y., Son, T. G., Yuk, D. Y., Jung, S. (2016). Silymarin prevents restraint stress-induced acute liver injury by ameliorating oxidative stress and reducing inflammatory response. Molecules 21, 443.
[16]	Lauwers, M., Croubels, S., Letor, B., Gougoulias, C., Devreese, M. (2019). Biomarkers for exposure as a tool for efficacy testing of a mycotoxin detoxifier in broiler chickens and pigs. Toxins, 11, 187.
[17]	Livak, K. J., Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2 (-Delta Delta C(T)) Method. Methods, 25 (4), 402-8.
[18]	Liu Y, Liu Q, Ye G, Khan A, Liu J, Gan F, et al. (2015). Protective effects of Selenium-enriched probiotics on carbon tetrachloride-induced liver fibrosis in rats. J Agric Food Chem; 63, 242.
[19]	Mantovani, M. S., Bellini, M. F., Angeli, J. P., Oliveira, R. J., Silva, A. F., Ribeiro, L. R. (2008). Beta-Glucans in promoting health: prevention against mutation and cancer. Mutat Res., 658 (3), 154-61.
[20]	Moore, K. W., Waal Malefyt, R. de, Coffman, R. L., & O’Garra, A. (2001). Interleukin-10 and the interleukin-10 receptor. Annual review of immunology, 19, 683-765.
[21]	Ning X., Jian Z., Wang W. (2015). Low Serum Levels of Interleukin 35 in Patients with Rheumatoid Arthritis. Tohoku J. Exp. Med., 237, 77-82.
[22]	National Research CouncilL. Nutrient requirements of poultry. 9 th ed. Washington, D.C.: National Academy of Science, 1994. 154 p.
[23]	Qu D., Huang X., Han J., Man N. (2017). Efficacy of mixed adsorbent in ameliorating ochratoxicosis in broilers fed ochratoxin A contaminated diets. Ital J Anim Sci. 16, 573–579.
[24]	Sattler N., Monroy R., Soldati T. (2013) Quantitative Analysis of Phagocytosis and Phagosome Maturation. In: Eichinger L., Rivero F. (eds) Dictyostelium discoideum Protocols. Methods in Molecular Biology (Methods and Protocols), vol 983. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-302-2_21
[25]	Shahbazi, F., Dashti-Khavidaki, S., Khalili, H., Lessan-Pezeshki, M., (2012). Potential renoprotective effects of silymarin against nephrotoxic drugs: A review of literature. Journal of Pharmacy and Pharmaceutical Sciences, 15, 112-123.
[26]	Soltanian, S., Stuyven, E., Cox, E., Sorgeloos, P. and Bossier, P. (2009) Beta-glucans as immunostimulant in vertebrates and invertebrates. Crit. Rev. Microbiol., 35, 109-138.
[27]	Trinchieri, G. (2007). Interleukin-10 production by effector T cells: Th1 cells show self-control. The Journal of experimental medicine, 204 (2), 239-43.
[28]	Tzianabos, A. O. (2000). Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function. Clinic. Microbiol., 13, 523-533.
[29]	Wang L, Huang QH, Li YX, et al. Protective effects of silymarin on triptolide-induced acute hepatotoxicity in rats. Mol Med Rep. 2018; 17 (1): 789-800. doi: 10.3892/mmr.2017.7958.
[30]	Yiannikouris A., François J., Poughon L., Dussap C. G., Bertin G., Jeminet G., Jouany J. P. (2004). Adsorption of zearalenone by beta-D-glucans in the Saccharomyces cerevisiae cell wall. J. Food Prot. 67, 1195-1200.
[31]	Zabriskie, Hannah A., Julia C. Blumkaitis, Jessica M. Moon, Brad S. Currier, Riley Stefan, Kayla Ratliff, and Chad M. Kerksick (2020). Yeast Beta-Glucan Supplementation Downregulates Markers of Systemic Inflammation after Heated Treadmill Exercise. Nutrients, 12 (4), 1144.

Cite This Article

Plain Text BibTeX RIS

APA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. (2022). Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Animal and Veterinary Sciences, 10(4), 78-82. https://doi.org/10.11648/j.avs.20221004.11

Copy | Download

ACS Style

Verônica Lisboa Santos; Juliana Bueno da Silva; Franciane Cristina de Figueiredo; Carlos Paulo Henrique Ronchi; Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim. Vet. Sci. 2022, 10(4), 78-82. doi: 10.11648/j.avs.20221004.11

Copy | Download

AMA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim Vet Sci. 2022;10(4):78-82. doi: 10.11648/j.avs.20221004.11

Copy | Download

@article{10.11648/j.avs.20221004.11,
  author = {Verônica Lisboa Santos and Juliana Bueno da Silva and Franciane Cristina de Figueiredo and Carlos Paulo Henrique Ronchi and Edenilse Gopinger},
  title = {Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins},
  journal = {Animal and Veterinary Sciences},
  volume = {10},
  number = {4},
  pages = {78-82},
  doi = {10.11648/j.avs.20221004.11},
  url = {https://doi.org/10.11648/j.avs.20221004.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20221004.11},
  abstract = {Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins
AU  - Verônica Lisboa Santos
AU  - Juliana Bueno da Silva
AU  - Franciane Cristina de Figueiredo
AU  - Carlos Paulo Henrique Ronchi
AU  - Edenilse Gopinger
Y1  - 2022/07/26
PY  - 2022
N1  - https://doi.org/10.11648/j.avs.20221004.11
DO  - 10.11648/j.avs.20221004.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 78
EP  - 82
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20221004.11
AB  - Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.
VL  - 10
IS  - 4
ER  -

Copy | Download

Author Information

Verônica Lisboa Santos
Juliana Bueno da Silva

Yessinergy do Brasil Agroindustrial Ltda, S?o Paulo, Brazil
Franciane Cristina de Figueiredo

Yessinergy do Brasil Agroindustrial Ltda, S?o Paulo, Brazil
Carlos Paulo Henrique Ronchi

Yessinergy do Brasil Agroindustrial Ltda, S?o Paulo, Brazil
Edenilse Gopinger

Zootechnics Department, Federal University of Pelotas, Pelotas, Brazil

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. (2022). Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Animal and Veterinary Sciences, 10(4), 78-82. https://doi.org/10.11648/j.avs.20221004.11

Copy | Download

ACS Style

Verônica Lisboa Santos; Juliana Bueno da Silva; Franciane Cristina de Figueiredo; Carlos Paulo Henrique Ronchi; Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim. Vet. Sci. 2022, 10(4), 78-82. doi: 10.11648/j.avs.20221004.11

Copy | Download

AMA Style

Verônica Lisboa Santos, Juliana Bueno da Silva, Franciane Cristina de Figueiredo, Carlos Paulo Henrique Ronchi, Edenilse Gopinger. Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins. Anim Vet Sci. 2022;10(4):78-82. doi: 10.11648/j.avs.20221004.11

Copy | Download

@article{10.11648/j.avs.20221004.11,
  author = {Verônica Lisboa Santos and Juliana Bueno da Silva and Franciane Cristina de Figueiredo and Carlos Paulo Henrique Ronchi and Edenilse Gopinger},
  title = {Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins},
  journal = {Animal and Veterinary Sciences},
  volume = {10},
  number = {4},
  pages = {78-82},
  doi = {10.11648/j.avs.20221004.11},
  url = {https://doi.org/10.11648/j.avs.20221004.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20221004.11},
  abstract = {Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.},
 year = {2022}
}

Copy | Download

TY  - JOUR
T1  - Immunological Parameters from Broiler Chickens Supplemented with Adsorbents and Challenged with Mycotoxins
AU  - Verônica Lisboa Santos
AU  - Juliana Bueno da Silva
AU  - Franciane Cristina de Figueiredo
AU  - Carlos Paulo Henrique Ronchi
AU  - Edenilse Gopinger
Y1  - 2022/07/26
PY  - 2022
N1  - https://doi.org/10.11648/j.avs.20221004.11
DO  - 10.11648/j.avs.20221004.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 78
EP  - 82
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20221004.11
AB  - Mycotoxins are one of the most immunosupressive factors in animal diets. It is important to consider that the consumption of certain mycotoxins, at levels that are not cause overt clinical mycotoxicosis, suppresses immune function and decrease resistance infectious disease. In this sense, mycotoxin adsorbents that are effective both in combating the damage caused by these metabolites and in protecting and supporting animal health are increasingly essential in the animal nutrition industry. It has been evaluated the effect of mycotoxin adsorbents, with different compositions, on the immune parameters in broiler chickens challenged with mycotoxins. We have utilized 300 broilers, distributed in a completely randomized design with four treatments and six replications. Three mycotoxin adsorbents with different compositions were tested, which were added 2.5kg/kg, in diets that were contaminated with 1.0 pp aflatoxin + 50.0 ppm fumonisin + 25.0 ppm DON. Evaluating a control diet without contamination (T1), contaminated diet + YES - FIX HP (T2), contaminated diet + adsorbent A (T3), contaminated diet + adsorbent B (T4). On the last day of the experimental period, blood samples were collected from eight birds per treatment for the evaluation of phagocytosis by flow cytometry and the cecal tonsils were collected and determined the expression of interleukin 6 pro-inflammatory (IL 6) and interleukin 10 anti-inflammatory (IL 10) by quantifying the gene expression by RT-qPCR. In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6 (pro-inflammatory). In the phagocytosis assay, a significant difference was observed between the control and T4 treatments, with a lower percentage of phagocytic monocytes for treatment 4, which also presented the lowest value of IL-6. Among the adsorbents evaluated, YES FIX HP provided the highest average production of IL-10, which suggests a greater balance in the immune response of animals to the challenge with mycotoxins, possibly contributing to the greater resistance of this group of animals to the main challenges faced in the field and that require a response from the defense system.
VL  - 10
IS  - 4
ER  -

Copy | Download