In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants

Julienne Kuiseu; Pascal Abiodoun Olounlade; Claude Gbemeho Houssoukpe; Tony Taofick Babalola Abiodoun Sounkere; Frejus Tanguy Ablo Zinsou; Basile Saka Boni Konmy; Christian Cocou Dansou; Ibikounle Moudachirou; Severin Babatounde; Sylvie Mawule Hounzangbe-Adote; Patrick Aleodjrodo Edorh

doi:doi:10.11648/j.avs.20221001.11

In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants

Julienne Kuiseu, Pascal Abiodoun Olounlade, Claude Gbemeho Houssoukpe, Tony Taofick Babalola Abiodoun Sounkere, Frejus Tanguy Ablo Zinsou, Basile Saka Boni Konmy, Christian Cocou Dansou, Ibikounle Moudachirou, Severin Babatounde, Sylvie Mawule Hounzangbe-Adote, Patrick Aleodjrodo Edorh

Published in Animal and Veterinary Sciences (Volume 10, Issue 1)

Received: 17 October 2021 Accepted: 25 December 2021 Published: 8 January 2022

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Abstract

This study evaluated the in vitro anthelmintic activity of aqueous and hydroethanolic extracts of Anogeissus leiocarpus and Adansonia digitata leaf powder at 75; 150; 300; 600; 1200 and 2400 µg/mL against H. contortus, using the egg hatch test and the adult worm motility inhibition test. For egg hatch inhibition, the both plants showed significant concentration-dependent efficacy and were significantly more effective (p < 0.001) at the highest concentrations (1200 and 2400 µg/mL) than at the lowest. The hydro ethanolic extract of A. leiocarpus showed the highest inhibition (R² = 0.9963) and the aqueous extract of the same plant showed the lowest inhibition (R² = 0.9742). The 50% inhibitory concentration (IC50) of the A. leiocarpus aqueous and hydro ethanolic extracts (86.19 and 72.5 µg/mL) were 3 times lower than those of the A. digitata extracts (302 and 269.5 µg/mL). Mobility of adult worms was also concentration dependent (p<0.05); and also dependent on incubation time (p<0.01). At 2400 μg/mL, all adult worms were immobile after 6 h of exposure, whereas at the lowest concentration (75 μg/mL), all adult worms were immobile after 36 h of exposure. Extracts from both plants had similar activity. Finally, the results of the present study suggest that the both plants studied have anthelmintic properties in vitro which would explain their use by livestock farmers. However, in vivo tests should be performed to confirm these properties in vivo as well.

Published in	Animal and Veterinary Sciences (Volume 10, Issue 1)
DOI	10.11648/j.avs.20221001.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Haemonchus contortus, Two Life-cycle Stages, Anogeissus leiocarpus, Adansonia digitate, Anthelmintic, In vitro Methods

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Julienne Kuiseu, Pascal Abiodoun Olounlade, Claude Gbemeho Houssoukpe, Tony Taofick Babalola Abiodoun Sounkere, Frejus Tanguy Ablo Zinsou, et al. (2022). In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants. Animal and Veterinary Sciences, 10(1), 1-7. https://doi.org/10.11648/j.avs.20221001.11

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ACS Style

Julienne Kuiseu; Pascal Abiodoun Olounlade; Claude Gbemeho Houssoukpe; Tony Taofick Babalola Abiodoun Sounkere; Frejus Tanguy Ablo Zinsou, et al. In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants. Anim. Vet. Sci. 2022, 10(1), 1-7. doi: 10.11648/j.avs.20221001.11

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AMA Style

Julienne Kuiseu, Pascal Abiodoun Olounlade, Claude Gbemeho Houssoukpe, Tony Taofick Babalola Abiodoun Sounkere, Frejus Tanguy Ablo Zinsou, et al. In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants. Anim Vet Sci. 2022;10(1):1-7. doi: 10.11648/j.avs.20221001.11

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@article{10.11648/j.avs.20221001.11,
  author = {Julienne Kuiseu and Pascal Abiodoun Olounlade and Claude Gbemeho Houssoukpe and Tony Taofick Babalola Abiodoun Sounkere and Frejus Tanguy Ablo Zinsou and Basile Saka Boni Konmy and Christian Cocou Dansou and Ibikounle Moudachirou and Severin Babatounde and Sylvie Mawule Hounzangbe-Adote and Patrick Aleodjrodo Edorh},
  title = {In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants},
  journal = {Animal and Veterinary Sciences},
  volume = {10},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.avs.20221001.11},
  url = {https://doi.org/10.11648/j.avs.20221001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20221001.11},
  abstract = {This study evaluated the in vitro anthelmintic activity of aqueous and hydroethanolic extracts of Anogeissus leiocarpus and Adansonia digitata leaf powder at 75; 150; 300; 600; 1200 and 2400 µg/mL against H. contortus, using the egg hatch test and the adult worm motility inhibition test. For egg hatch inhibition, the both plants showed significant concentration-dependent efficacy and were significantly more effective (p A. leiocarpus showed the highest inhibition (R2 = 0.9963) and the aqueous extract of the same plant showed the lowest inhibition (R2 = 0.9742). The 50% inhibitory concentration (IC50) of the A. leiocarpus aqueous and hydro ethanolic extracts (86.19 and 72.5 µg/mL) were 3 times lower than those of the A. digitata extracts (302 and 269.5 µg/mL). Mobility of adult worms was also concentration dependent (pin vitro which would explain their use by livestock farmers. However, in vivo tests should be performed to confirm these properties in vivo as well.},
 year = {2022}
}

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TY  - JOUR
T1  - In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants
AU  - Julienne Kuiseu
AU  - Pascal Abiodoun Olounlade
AU  - Claude Gbemeho Houssoukpe
AU  - Tony Taofick Babalola Abiodoun Sounkere
AU  - Frejus Tanguy Ablo Zinsou
AU  - Basile Saka Boni Konmy
AU  - Christian Cocou Dansou
AU  - Ibikounle Moudachirou
AU  - Severin Babatounde
AU  - Sylvie Mawule Hounzangbe-Adote
AU  - Patrick Aleodjrodo Edorh
Y1  - 2022/01/08
PY  - 2022
N1  - https://doi.org/10.11648/j.avs.20221001.11
DO  - 10.11648/j.avs.20221001.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20221001.11
AB  - This study evaluated the in vitro anthelmintic activity of aqueous and hydroethanolic extracts of Anogeissus leiocarpus and Adansonia digitata leaf powder at 75; 150; 300; 600; 1200 and 2400 µg/mL against H. contortus, using the egg hatch test and the adult worm motility inhibition test. For egg hatch inhibition, the both plants showed significant concentration-dependent efficacy and were significantly more effective (p A. leiocarpus showed the highest inhibition (R2 = 0.9963) and the aqueous extract of the same plant showed the lowest inhibition (R2 = 0.9742). The 50% inhibitory concentration (IC50) of the A. leiocarpus aqueous and hydro ethanolic extracts (86.19 and 72.5 µg/mL) were 3 times lower than those of the A. digitata extracts (302 and 269.5 µg/mL). Mobility of adult worms was also concentration dependent (pin vitro which would explain their use by livestock farmers. However, in vivo tests should be performed to confirm these properties in vivo as well.
VL  - 10
IS  - 1
ER  -

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Author Information

Julienne Kuiseu

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Pascal Abiodoun Olounlade

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Claude Gbemeho Houssoukpe

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Tony Taofick Babalola Abiodoun Sounkere

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Frejus Tanguy Ablo Zinsou

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Basile Saka Boni Konmy

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Christian Cocou Dansou

Zootechnics and Livestock Systems Research Unit (URZoSE), Animal and Fisheries Sciences Laboratory (LaSAH), Doctoral School of Agronomic and Water Sciences (EDSAE), National University of Agriculture (UNA), Porto-Novo, Republic of Benin
Ibikounle Moudachirou

Zoology Laboratory of the Faculty of Science and Technology, University of Abomey-Calavi (UAC), Cotonou, Republic of Benin
Severin Babatounde

Laboratory of Zootechnics, Faculty of Agronomic Sciences, University of Abomey-Calavi (UAC), Cotonou, Republic of Benin
Sylvie Mawule Hounzangbe-Adote

Laboratory of Ethnopharmacology and Animal Health, Faculty of Agronomic Sciences, University of Abomey Calavi (UAC), Cotonou, Republic of Benin
Patrick Aleodjrodo Edorh

Laboratory of Toxicology and Environmental Health (LATSE), University of Abomey-Calavi (UAC), Cotonou, Republic of Benin

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Julienne Kuiseu, Pascal Abiodoun Olounlade, Claude Gbemeho Houssoukpe, Tony Taofick Babalola Abiodoun Sounkere, Frejus Tanguy Ablo Zinsou, et al. (2022). In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants. Animal and Veterinary Sciences, 10(1), 1-7. https://doi.org/10.11648/j.avs.20221001.11

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ACS Style

Julienne Kuiseu; Pascal Abiodoun Olounlade; Claude Gbemeho Houssoukpe; Tony Taofick Babalola Abiodoun Sounkere; Frejus Tanguy Ablo Zinsou, et al. In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants. Anim. Vet. Sci. 2022, 10(1), 1-7. doi: 10.11648/j.avs.20221001.11

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AMA Style

Julienne Kuiseu, Pascal Abiodoun Olounlade, Claude Gbemeho Houssoukpe, Tony Taofick Babalola Abiodoun Sounkere, Frejus Tanguy Ablo Zinsou, et al. In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants. Anim Vet Sci. 2022;10(1):1-7. doi: 10.11648/j.avs.20221001.11

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@article{10.11648/j.avs.20221001.11,
  author = {Julienne Kuiseu and Pascal Abiodoun Olounlade and Claude Gbemeho Houssoukpe and Tony Taofick Babalola Abiodoun Sounkere and Frejus Tanguy Ablo Zinsou and Basile Saka Boni Konmy and Christian Cocou Dansou and Ibikounle Moudachirou and Severin Babatounde and Sylvie Mawule Hounzangbe-Adote and Patrick Aleodjrodo Edorh},
  title = {In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants},
  journal = {Animal and Veterinary Sciences},
  volume = {10},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.avs.20221001.11},
  url = {https://doi.org/10.11648/j.avs.20221001.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20221001.11},
  abstract = {This study evaluated the in vitro anthelmintic activity of aqueous and hydroethanolic extracts of Anogeissus leiocarpus and Adansonia digitata leaf powder at 75; 150; 300; 600; 1200 and 2400 µg/mL against H. contortus, using the egg hatch test and the adult worm motility inhibition test. For egg hatch inhibition, the both plants showed significant concentration-dependent efficacy and were significantly more effective (p A. leiocarpus showed the highest inhibition (R2 = 0.9963) and the aqueous extract of the same plant showed the lowest inhibition (R2 = 0.9742). The 50% inhibitory concentration (IC50) of the A. leiocarpus aqueous and hydro ethanolic extracts (86.19 and 72.5 µg/mL) were 3 times lower than those of the A. digitata extracts (302 and 269.5 µg/mL). Mobility of adult worms was also concentration dependent (pin vitro which would explain their use by livestock farmers. However, in vivo tests should be performed to confirm these properties in vivo as well.},
 year = {2022}
}

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TY  - JOUR
T1  - In vitro Effects of Anogeissus leiocarpus and Adansonia digitata on Two Life-cycle Stages of Haemonchus contortus, a Gastrointestinal Parasite of Small Ruminants
AU  - Julienne Kuiseu
AU  - Pascal Abiodoun Olounlade
AU  - Claude Gbemeho Houssoukpe
AU  - Tony Taofick Babalola Abiodoun Sounkere
AU  - Frejus Tanguy Ablo Zinsou
AU  - Basile Saka Boni Konmy
AU  - Christian Cocou Dansou
AU  - Ibikounle Moudachirou
AU  - Severin Babatounde
AU  - Sylvie Mawule Hounzangbe-Adote
AU  - Patrick Aleodjrodo Edorh
Y1  - 2022/01/08
PY  - 2022
N1  - https://doi.org/10.11648/j.avs.20221001.11
DO  - 10.11648/j.avs.20221001.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20221001.11
AB  - This study evaluated the in vitro anthelmintic activity of aqueous and hydroethanolic extracts of Anogeissus leiocarpus and Adansonia digitata leaf powder at 75; 150; 300; 600; 1200 and 2400 µg/mL against H. contortus, using the egg hatch test and the adult worm motility inhibition test. For egg hatch inhibition, the both plants showed significant concentration-dependent efficacy and were significantly more effective (p A. leiocarpus showed the highest inhibition (R2 = 0.9963) and the aqueous extract of the same plant showed the lowest inhibition (R2 = 0.9742). The 50% inhibitory concentration (IC50) of the A. leiocarpus aqueous and hydro ethanolic extracts (86.19 and 72.5 µg/mL) were 3 times lower than those of the A. digitata extracts (302 and 269.5 µg/mL). Mobility of adult worms was also concentration dependent (pin vitro which would explain their use by livestock farmers. However, in vivo tests should be performed to confirm these properties in vivo as well.
VL  - 10
IS  - 1
ER  -

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