Bees and their larvae are susceptible to various viral diseases, which leads to disruption of the bee family, and sometimes to its death. The Sacbrood virus is especially dangerous for the bee family. Sick families differ sharply in their condition and productivity from healthy ones, develop slowly in the spring, and grow little brood. Therefore, the aim of the study was to identify physiological changes in the intestines of mature uteruses affected by Sacbrood virus or SBV disease. Histological examinations supplemented the data on the pathogenesis of honey bees affected by Sacbrood virus. Data on physiological and morphological changes in the middle part of the intestine of fertile queens are presented. Histological examinations were performed in different parts of the midgut. In patients of queens, in comparison with clinically healthy ones, changes in the function of the pertrophic membrane were revealed. An increase in the length of the midgut fold in the anterior part of the intestine by 23% (p<0.001) was detected. Under the influence of pathogenicity of the virus there is an intensive proliferation of enterocytes in the middle part of the intestine. The defeat of the structural components of the mucosa is evidenced by the absence of a peretrophic membrane in the caudal direction of the midgut of diseased queens where necrotic lesions of the mucous membrane up to the basement membrane were detected.
| Published in | Animal and Veterinary Sciences (Volume 9, Issue 3) |
| DOI | 10.11648/j.avs.20210903.15 |
| Page(s) | 73-79 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Apis mellifera L., Sacbrood virus (SBV), Fertile Queens, Midgut, Physiology Nutrition
| [1] | Gallai N, Salles JM, Settele J, Vaissière B. 2009. Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecol. Econ. 68 (3): 810–821. |
| [2] | Tanada Y, Kaya H. 1992. Insect Pathology. University of California: Academic Press. |
| [3] | Ribière M, Faucon J, Pépin M. 2000. Detection of chronic honey bee (Apis mellifera L.) paralysis virus infection: application to a field survey. Apidologie. 31 (5): 567–577. |
| [4] | Bakonyi T, Grabensteiner E, Kolodziejek J, Rusvai M, Topolska G, Ritter W, Nowotny N. 2002. Phylogenetic analysis of acute bee paralysis virus strains. Appl. Environ. Microbiol. 68 (12): 6446–6450. |
| [5] | Ellis JD, Evans JD, Pettis J. 2010. Colony losses, managed colony population decline, and Colony Collapse Disorder in the United States. J. Apic. Res. 49: 134–136. |
| [6] | Jones B, Shipley E, Kathryn A. 2018. Social immunity in honeybees—Density dependence, diet, and body mass trade-offs. Ecol Evol. 8 (10): 4852–4859. |
| [7] | Evans J, Aronstein K, Chen Y, Hetru C, Imler J, Jiang H, Kanost M, Thompson G, Zou Z, Hultmark D. 2006. Immune pathways and defence mechanisms in honey bees Apis mellifera. Insect Molecular Biology. 15: 645-656. |
| [8] | Alaux C, Crauser D, Pioz M, Saulnier C & Le Conte Y. 2014. Parasitic and immune modulation of flight activity in honey bees tracked with optical counters. Journal of Experimental Biology. 217 (19): 3416–3424. |
| [9] | Bailey L, Gibbs A, Woods R. 1964. Sacbrood virus of the larval honey bee (Apis mellifera Linnaeus). Virology. 23: 425-429. |
| [10] | Bailey L. 1969. The multiplication and spread of Sacbrood virus of bees Apis mellifera. Annals of Applied Biology. 63: 483-491. |
| [11] | Blanchard P, Guillot S, Antunez K, Koeglberger H, Kryger P, De Miranda JR, Franco S, Chauzat MP, Thiery R, Ribiere M. 2014. Development and validation of a real-time two-step RT-qPCR TaqMan (R) assay for quantitation of Sacbrood virus (SBV) and its application to a field survey of symptomatic honey bee colonies. Journal of Virological Methods. 197: 7-13. |
| [12] | Ai H, Yan X, Han R. 2012. Occurrence and prevalence of seven bee viruses in Apis mellifera and Apis cerana apiaries in China. Journal of Invertebrate Pathology. 109: 160-164. |
| [13] | Li JN, Wang T, Evans J, Rose R, Zhao Y, Li Z, Li JL, Huang S, Heerman M, Rodríguez-García C, et al. 2019. The phylogeny and pathogenesis of sacbrood virus (SBV) infection in European Honey Bees, Apis mellifera. Viruses. 11 (1): 61. |
| [14] | Rao K, Katna S, Rana B, Rana R. 2019. Thai sacbrood and sacbrood viruses versus European foulbrood of hive bees in India. A review. Journal of Apicultural Research. 54 (3): 1-8. |
| [15] | Grabensteiner E, Ritter W, Carter M, Davison S, Pechhacker H, Kolodziejek J, Boecking O, Derakhshifar I, Moosbeckhofer R, Licek E, et al. 2001. Sacbrood Virus of the Honeybee (Apis mellifera): Rapid Identification and Phylogenetic Analysis Using Reverse Transcription-PCR. Clinical and Diagnostic Laboratory Immunology. 8 (1): 93–104. |
| [16] | Horal’sʹkyy L, Khomych V, Konons’kyy O. 2005. Osnovy istorychnoyi tekhniky ta morfofunktsional’ni metody doslidzhennya u normi ta pry patolohiyi. Zhytomyr: Polissya. |
| [17] | Plokhynskyy NA. 1969. Rukovodstvo po byometryy dlya zootekhnykov. Moscow: Kolos. p. 25–27. |
| [18] | Snodgrass RE. 1910. The anatomy of the honey bee. Washington: government printing office. |
| [19] | Tentcheva D, Gauthier L, Zappulla N, Dainat B, Cousserans F, Colin ME, Bergoin M. 2004. Prevalence and seasonal variations of six bee viruses in Apis mellifera L. and Varroa destructor mite populations in France. Appl Environ Microbiol. 70: 7185–7191. |
| [20] | Antúnez K, D'Alessandro B, Corbella E, Ramallo G, Zunino P. 2006. Honeybee viruses in Uruguay. J Invertebr Pathol. 93: 67–70. |
| [21] | Liu TP. 1984. Ultrastructure of the midgut of the worker honey bee Apis mellifera heavily infected with Nosema apis. Journal of Invertebrate Pathology. 44 (3): 282-291. |
| [22] | Maiolino P, Iafigliola L, Giovanna De Leva, Rinaldi L. 2014. Histopathological findings of the midgut in European honey bee (Apis Mellifera L.) naturally infected by Nosema spp. Veterinary Medicine and Animal Sciences. 2 (1): 4. |
| [23] | Van Engelsdorp D, Traynor K, Andree M. 2017. Colony Collapse Disorder (CCD) and bee age impact honey bee pathophysiology. PLoS ONE. 12 (7). |
| [24] | Moritz B, Crailsheim K. 1987. Physiology of protein digestion in the midgut of the honeybee (Apis mellifera L.). Journal of Insect Physiology. 33 (12): 923–931. |
| [25] | Stell I. 2014. - Pollen for lunch? Again? Bee Culture. November. |
| [26] | Jimenez DR, Gilliam M. 1989. Age-related changes in midgut ultrastructure and trypsin activity in the honey bee, Apis mellifera. Apidologie. 20: 287-303. |
| [27] | Xia X, Zhou B, Wei T. 2015. Complete genome of Chinese sacbrood virus from Apis cerana and analysis of the 3C-like cysteine protease. Virus Genes. 50 (2): 277-285. |
| [28] | Evans J, Lopez D. 2004. Bacterial Probiotics Induce an Immune Response in the Honey Bee (Hymenoptera: Apidae). Journal of Economic Entomology. 97 (3): 752–756. |
| [29] | Ilyasov R, Gaifullina L, Saltykova E, Poskryakov A, Nikolenko A. 2012. Review of the Expression of Antimicrobial Peptide Defensin in Honey Bees Apis Mellifera L. Journal of Apicultural Science. 56 (1): 115-124. |
| [30] | Mussen E, Furgala В. 1977. Replication of sacbrood virus in larval and adult honeybees, Apis mellifera. Journal of Invertebrate Pathology. 30 (1): 20-34. |
| [31] | Denholm C. 1999. Inducible honey bee viruses associated with Varroa jacobsoni. [dissertation]. Keele University. |
| [32] | Fievet J, Tentcheva D, Gauthier L, Miranda J, Cousserans F, Colin M, Bergoin M. 2006. Localization of deformed wing virus infection in queen and drone Apis mellifera L. Virology Journal. 3 (16): 1-5. |
| [33] | Bull J, Ryabov E, Prince G, Mead A, Zhang C, Baxter L, Pell J, Osborne J, Chandler D. 2012. A strong immune response in young adult honeybees masks their increased susceptibility to infection compared to older bees. Plos Pathogens. 8 (12): 1-11. |
| [34] | Ryabov E, Fannon J, Moore J, Wood G, Evans D. 2016. The Iflaviruses Sacbrood virus and Deformed wing virus evoke different transcriptional responses in the honeybee which may facilitate their horizontal or vertical transmission. PeerJ. 4: 1591. |
| [35] | Norman L, Carreck, Brenda V, Ball, Stephen J, Martin. 2010. The epidemiology of cloudy wing virus infections in honey bee colonies in the UK. Journal of Apicultural Research. 49 (1): 66–71. |
| [36] | Hrobov O, Lykhotyn A. 1989. Bolezny i vredytely pchel. Moscow: Kolos. p. 148–156. |
| [37] | Nazzi F, Pennacchio F. 2014. Disentangling multiple interactions in the hive ecosystem. Trends Parasitol. 30: 556–561. |
| [38] | Deng Y, Zhao H, Shen S, Yang S, Yang D, Deng S and Hou C (2020) Identification of Immune Response to Sacbrood Virus Infection in Apis cerana Under Natural Condition. Front. Genet. 11: 587509. |
| [39] | Jin L., Mehmood S., Zhang G., Song Y., Su S., Huang S., Huang H., Zhang Y., Geng H., Huang W. (2000). Visualizing Sacbrood Virus of Honey Bees via Transformation and Coupling with Enhanced Green Fluorescent Protein. Viruses 2020, 12 (2), 224. |
| [40] | Sun, L., Zhang, X., Xu, S. et al. (2021). Antiviral Activities of a Medicinal Plant Extract Against Sacbrood Virus in Honeybees. Virol J 18, 83. |
| [41] | Salina, M. D., Garcia, M. L. G., Bais, B. et al. (2021). Viruses that affect Argentinian honey bees (Apis mellifera). Arch Virol 166, 1533–1545. |
APA Style
Iurii Kovalskyi, Vasylyna Fedak, Lidiya Kovalska, Andrii Druzhbiak, Yaroslav Vovkun. (2021). Structure of the Midgut of the Queen Bee Apis mellifera L. Naturally Infected with Sacbrood virus (SBV). Animal and Veterinary Sciences, 9(3), 73-79. https://doi.org/10.11648/j.avs.20210903.15
ACS Style
Iurii Kovalskyi; Vasylyna Fedak; Lidiya Kovalska; Andrii Druzhbiak; Yaroslav Vovkun. Structure of the Midgut of the Queen Bee Apis mellifera L. Naturally Infected with Sacbrood virus (SBV). Anim. Vet. Sci. 2021, 9(3), 73-79. doi: 10.11648/j.avs.20210903.15
AMA Style
Iurii Kovalskyi, Vasylyna Fedak, Lidiya Kovalska, Andrii Druzhbiak, Yaroslav Vovkun. Structure of the Midgut of the Queen Bee Apis mellifera L. Naturally Infected with Sacbrood virus (SBV). Anim Vet Sci. 2021;9(3):73-79. doi: 10.11648/j.avs.20210903.15
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Download@article{10.11648/j.avs.20210903.15,
author = {Iurii Kovalskyi and Vasylyna Fedak and Lidiya Kovalska and Andrii Druzhbiak and Yaroslav Vovkun},
title = {Structure of the Midgut of the Queen Bee Apis mellifera L. Naturally Infected with Sacbrood virus (SBV)},
journal = {Animal and Veterinary Sciences},
volume = {9},
number = {3},
pages = {73-79},
doi = {10.11648/j.avs.20210903.15},
url = {https://doi.org/10.11648/j.avs.20210903.15},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20210903.15},
abstract = {Bees and their larvae are susceptible to various viral diseases, which leads to disruption of the bee family, and sometimes to its death. The Sacbrood virus is especially dangerous for the bee family. Sick families differ sharply in their condition and productivity from healthy ones, develop slowly in the spring, and grow little brood. Therefore, the aim of the study was to identify physiological changes in the intestines of mature uteruses affected by Sacbrood virus or SBV disease. Histological examinations supplemented the data on the pathogenesis of honey bees affected by Sacbrood virus. Data on physiological and morphological changes in the middle part of the intestine of fertile queens are presented. Histological examinations were performed in different parts of the midgut. In patients of queens, in comparison with clinically healthy ones, changes in the function of the pertrophic membrane were revealed. An increase in the length of the midgut fold in the anterior part of the intestine by 23% (p<0.001) was detected. Under the influence of pathogenicity of the virus there is an intensive proliferation of enterocytes in the middle part of the intestine. The defeat of the structural components of the mucosa is evidenced by the absence of a peretrophic membrane in the caudal direction of the midgut of diseased queens where necrotic lesions of the mucous membrane up to the basement membrane were detected.},
year = {2021}
}
TY - JOUR T1 - Structure of the Midgut of the Queen Bee Apis mellifera L. Naturally Infected with Sacbrood virus (SBV) AU - Iurii Kovalskyi AU - Vasylyna Fedak AU - Lidiya Kovalska AU - Andrii Druzhbiak AU - Yaroslav Vovkun Y1 - 2021/06/25 PY - 2021 N1 - https://doi.org/10.11648/j.avs.20210903.15 DO - 10.11648/j.avs.20210903.15 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 73 EP - 79 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20210903.15 AB - Bees and their larvae are susceptible to various viral diseases, which leads to disruption of the bee family, and sometimes to its death. The Sacbrood virus is especially dangerous for the bee family. Sick families differ sharply in their condition and productivity from healthy ones, develop slowly in the spring, and grow little brood. Therefore, the aim of the study was to identify physiological changes in the intestines of mature uteruses affected by Sacbrood virus or SBV disease. Histological examinations supplemented the data on the pathogenesis of honey bees affected by Sacbrood virus. Data on physiological and morphological changes in the middle part of the intestine of fertile queens are presented. Histological examinations were performed in different parts of the midgut. In patients of queens, in comparison with clinically healthy ones, changes in the function of the pertrophic membrane were revealed. An increase in the length of the midgut fold in the anterior part of the intestine by 23% (p<0.001) was detected. Under the influence of pathogenicity of the virus there is an intensive proliferation of enterocytes in the middle part of the intestine. The defeat of the structural components of the mucosa is evidenced by the absence of a peretrophic membrane in the caudal direction of the midgut of diseased queens where necrotic lesions of the mucous membrane up to the basement membrane were detected. VL - 9 IS - 3 ER -
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