Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens

Mohiuddin Amirul Kabir Chowdhury; Amrita Kumar Dhara; Shivaji Dey; Anirvid Sarkar; Sudipto Haldar; Glenmer Bathan Tactacan

doi:doi:10.11648/j.avs.20210901.13

Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens

Mohiuddin Amirul Kabir Chowdhury, Amrita Kumar Dhara, Shivaji Dey, Anirvid Sarkar, Sudipto Haldar, Glenmer Bathan Tactacan

Published in Animal and Veterinary Sciences (Volume 9, Issue 1)

Received: 3 February 2021 Accepted: 10 February 2021 Published: 17 March 2021

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Abstract

A 42-day trial was conducted to assess the effects of a dietary protease on growth performance and ileum population of selected bacteria in broiler chickens fed standard diets and diets deficient in crude protein and digestible amino acids (CP/AA, ~5%) or apparent metabolizable energy (AME, 50 kcal/kg) or both. Reducing CP/AA and AME negatively affected average daily weight gain (ADG, P < 0.05) and feed conversion ratio (FCR, P < 0.05). Dietary protease improved BW at 42-d (P = 0.021, linear effect) of birds fed both standard and nutrient deficient diets but improved FCR (P = 0.0002) was only observed when supplemented to the standard diet. Serum protein concentration was not affected by the level of CP/AA and AME but decreased linearly with the level of protease (P = 0.02). Numbers of Escherichia coli¸ Salmonella spp. and Clostridium perfringens increased (P = 0.0001) with the decrease of dietary CP/AA and AME. In contrast, the numbers of studied bacteria linearly decreased (P = 0.0001) with dietary protease level. Dietary protease was found to decrease the numbers of Clostridium perfringens (P = 0.0001) when supplemented to either low CP/AA or low AME diets. It can be concluded that protease supplementation sustained the harvesting biomass in the experimental birds fed lower nutrient density diets and decreased the numbers of resident bacteria in the hind gut, some of which might be potential pathogens. Hence, the study documented not only the nutrient sparing effects but also the extra-proteinaceous effect in terms of gut health of dietary protease in broiler chickens.

Published in	Animal and Veterinary Sciences (Volume 9, Issue 1)
DOI	10.11648/j.avs.20210901.13
Page(s)	16-23
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Protease, Amino Acids, Growth Performance, Microbiota, Broiler

References

[1]	Le Heurou-Luron I, Lhoste E, Wicker-Planquarl C, Dakka N, Toullec R, Corring T, et al. Molecular aspects of enzyme synthesis in the exocrine pancreas with emphasis on development and nutritional regulation. Proc. Nutr. Soc. 1993. 52: 301-313.
[2]	Nir I, Nitsan Z, Mahagna M. Comparative growth and development of the digestive organs and of some enzymes in broiler and egg type chicks after hatching. Br. Poult. Sci. 1993. 34: 523-532.
[3]	Parsons CM, Castanon F, Han Y. Protein and amino acid quality of meat and bone meal. Poult. Sci. 1997. 76: 361-368.
[4]	Wang X, Parsons CM. Effect of processing systems on protein quality of feather meals and hog hair meals. Poult. Sci. 1997. 76: 491-496.
[5]	Lemme A, Ravindran V, Bryden WL. Ileal digestibility of amino acids in feed ingredients for broilers. World Poult. Sci. J. 2004. 60: 423-437.
[6]	Angel CR, Saylor W, Vieira SL, Ward N. Effects of a monocomponent protease on performance and protein utilization in 7- to 22-day-old broiler chickens. Poult. Sci. 2011. 90: 2281-2286. https://doi.org/10.3382/ps.2011-01482
[7]	Apajalahti J, Vienola K. Interaction between chicken intestinal microbiota and protein digestion. Ani. Feed. Sci. Tech. 2016. 221: 323-330.
[8]	Wilkie DC, Van Kessel AG, White LJ, Laarveld B, Drew MD. Dietary amino acids affect intestinal Clostridium perfringens populations in broiler chickens. Can. J. Ani. Sci. 2005. 85: 185-193.
[9]	Cowieson AJ, Roos FF. Bioefficacy of a mono-component protease in the diets of pigs and poultry: a meta-analysis of effect on ileal amino acid digestibility. J. Appl. Anim. Nutr. 2014. 2: 13-21.
[10]	Yu B, Wu ST, Liu CC, Gauthier R, Chiou PWS. Effects of enzyme inclusion in a maize-soybean diet on broiler performance. Ani. Feed. Sci. 2007. 134: 283-294. http://dx.doi.org/10.1016/j.anifeedsci.2006.09.017
[11]	Zuo J, Ling B, Long L, Li T, Lahaye L, Yang C, et al. Effect of dietary supplementation with protease on growth performance, nutrient digestibility, intestinal morphology, digestive enzymes and gene expression of weaned piglets. An. Nut. 2015. 1: 276-282.
[12]	Tactacan GB, Oh S, Cho JH, Kim IH. Performance responses, nutrient digestibility, blood characteristics, and measures of gastrointestinal health in weanling pigs fed protease enzyme. Asian-Australas. J. Anim. Sci. 2016. 7: 998-1003.
[13]	Cowieson AJ, Toghyani M, Kheravii SK, Wu SB, Romero LF, Choct M. A mono-component microbial protease improves performance, net energy, and digestibility of amino acids and starch, and upregulates jejunal expression of genes responsible for peptide transport in broilers fed corn/wheat-based diets supplemented with xylanase and phytase. Poult. Sci. 2019. 98: 1321-1332.
[14]	Saleh AA, Dawood MM, Badawi NA, Ebeid TA, Amber KA, Azzam MM. Effect of supplemental serine-protease from Bacillus licheniformis on growth performance and physiological change of broiler chickens. App. Ani. Res. 2020. 48: 86-92.
[15]	Muthusamy N, Haldar S, Ghosh TK, Bedfor MR. Effect of hydrolysed Saccharomyces cerevisiae yeast and yeast cell wall components on live performance, intestinal histomorphology and humoral immune response of broilers. Brit. Poult. Sci. 2011. 52: 694-703.
[16]	Cardinal KM, De Moraes ML, Andretta I, Schirmann GD, Belote BL, Barrios MA, et al. Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease. R. Bras. Zootec. 2019. 48: 1-11. https://doi.org/10.1590/rbz4820180232
[17]	Dessimoni GV, Dalolio FS, Moreira J, Teixeira LV, Bertechini AG, Hermes RG. Protease supplementation under amino acid reduction in diets formulated with different nutritional requirements for broilers. Braz. J. Poult. Sci. 2019. 21 (1): 1-8.
[18]	Al-juboori J. Effect of protease supplementation in broiler feed on growth performance, carcass yield and total nitrogen retention in fecal matter and litter. M. Sc. Thesis. 2017. Stephen S. Austin University. 2017. Available from: https://scholarworks.sfasu.edu/etds/142/
[19]	Taelibhong M, Attamangkune S, Ruangpanit Y. Effect of using alternative protein feedstuffs with protease supplementation in diet on growth performance and apparent ileal protein digestibility of broilers. Mahanakorn Vet. Med. 2019. 14: 93-104.
[20]	Park JH, Kim IH. Effects of a protease and essential oils on growth performance, blood cell profiles, nutrient retention, ileal microbiota, excreta gas emission, and breast meat quality in broiler chicks. Poult. Sci. 2018. 97: 1854-2860.
[21]	Kamel NF, Raaga NM, El-Banna RA, Mohamed FF. Effects of a monocomponent protease on performance parameters and protein digestibility in broiler chickens. Agri. Agrisci. Proc. 2015. 6: 216-225. https://doi.org/10.1016/j.aaspro.2015.08.062
[22]	Naik RP, Reddy AR, Reddy KK, Jyothi J. Effects of encapsulated protease enzyme on the performance, carcass, serum protein and retention of protein and energy in broilers. Int. J. Livest. Res. 2017. 7: 220-227.
[23]	Law FL, Zulkifli I, Soleimani AF, Liang JB, Awad EA. The effects of low-protein diets and protease supplementation on broiler chickens in a hot and humid tropical environment. Asian-Australas. J. Anim. Sci. 2018. 31: 1291-1300.
[24]	Barekatain MR, Antipatis C, Rodger N, Walkden-Brown SW, Iji PA, Choct M. Evaluation of high dietary inclusion of distillers dried grains with solubles and supplementation of protease and xylanase in the diets of broiler chickens under necrotic enteritis challenge. Poult Sci. 2013. 92: 1579-1594.
[25]	Cowieson AJ, Zaefarian F, Knap I, Ravindran V. Interactive effects of dietary protein concentration, a mono-component exogenous protease and ascorbic acid on broiler performance, nutritional status and gut health. Anim. Prod. Sci. 2016. 57: 1058-1068. https://doi.org/10.1071/AN15740
[26]	Yan F, Dibner JJ, Knight CD, Vazquez-Anon M. Effect of carbohydrase and protease on growth performance and gut health of young broilers fed diets containing rye, wheat, and feather meal. Poult. Sci. 2017. 96: 817-828.
[27]	Cowieson AJ, Roos FF. Toward optimal value creation through the application of exogenous mono-component protease in the diets of non-ruminants. Ani. Feed. Sci. Tech. 2016. 221: 331-340.
[28]	Windey K, De Preter V, Verbeke K. Relevance of protein fermentation to gut health. Mol. Nutr. Food Res. 2012. 56: 184-196.
[29]	Liu SY, Selle PH, Court SG, Cowieson AJ. Protease supplementation of sorghum-based broiler diets enhances amino acid digestibility coefficients in four small intestinal sites and accelerates their rates of digestion. Anim. Feed Sci. Technol. 2013. 183: 175-183. https://doi.org/10.1016/j.anifeedsci.2013.05.006
[30]	Giannenas I, Bonos E, Anestis V, Filioussis G, Papanastasiou DK, Bartzanas T, et al. Effects of protease addition and replacement of soybean meal by corn gluten meal on the growth of broilers and on the environmental performances of a broiler production system in Greece. PLoS ONE. 2017. 12 (1): e0169511. doi: 10.1371/journal.pone.0169511.
[31]	Peek HW, Van Der Klis JD, Vermeulen B, Landman WJM. Dietary protease can alleviate negative effects of a coccidiosis infection on production performance in broiler chickens. Anim. Feed Sci. Technol. 2009. 150: 151-159.

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APA Style

Mohiuddin Amirul Kabir Chowdhury, Amrita Kumar Dhara, Shivaji Dey, Anirvid Sarkar, Sudipto Haldar, et al. (2021). Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens. Animal and Veterinary Sciences, 9(1), 16-23. https://doi.org/10.11648/j.avs.20210901.13

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ACS Style

Mohiuddin Amirul Kabir Chowdhury; Amrita Kumar Dhara; Shivaji Dey; Anirvid Sarkar; Sudipto Haldar, et al. Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens. Anim. Vet. Sci. 2021, 9(1), 16-23. doi: 10.11648/j.avs.20210901.13

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AMA Style

Mohiuddin Amirul Kabir Chowdhury, Amrita Kumar Dhara, Shivaji Dey, Anirvid Sarkar, Sudipto Haldar, et al. Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens. Anim Vet Sci. 2021;9(1):16-23. doi: 10.11648/j.avs.20210901.13

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@article{10.11648/j.avs.20210901.13,
  author = {Mohiuddin Amirul Kabir Chowdhury and Amrita Kumar Dhara and Shivaji Dey and Anirvid Sarkar and Sudipto Haldar and Glenmer Bathan Tactacan},
  title = {Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens},
  journal = {Animal and Veterinary Sciences},
  volume = {9},
  number = {1},
  pages = {16-23},
  doi = {10.11648/j.avs.20210901.13},
  url = {https://doi.org/10.11648/j.avs.20210901.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20210901.13},
  abstract = {A 42-day trial was conducted to assess the effects of a dietary protease on growth performance and ileum population of selected bacteria in broiler chickens fed standard diets and diets deficient in crude protein and digestible amino acids (CP/AA, ~5%) or apparent metabolizable energy (AME, 50 kcal/kg) or both. Reducing CP/AA and AME negatively affected average daily weight gain (ADG, P P P = 0.021, linear effect) of birds fed both standard and nutrient deficient diets but improved FCR (P = 0.0002) was only observed when supplemented to the standard diet. Serum protein concentration was not affected by the level of CP/AA and AME but decreased linearly with the level of protease (P = 0.02). Numbers of Escherichia coli¸ Salmonella spp. and Clostridium perfringens increased (P = 0.0001) with the decrease of dietary CP/AA and AME. In contrast, the numbers of studied bacteria linearly decreased (P = 0.0001) with dietary protease level. Dietary protease was found to decrease the numbers of Clostridium perfringens (P = 0.0001) when supplemented to either low CP/AA or low AME diets. It can be concluded that protease supplementation sustained the harvesting biomass in the experimental birds fed lower nutrient density diets and decreased the numbers of resident bacteria in the hind gut, some of which might be potential pathogens. Hence, the study documented not only the nutrient sparing effects but also the extra-proteinaceous effect in terms of gut health of dietary protease in broiler chickens.},
 year = {2021}
}

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TY  - JOUR
T1  - Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens
AU  - Mohiuddin Amirul Kabir Chowdhury
AU  - Amrita Kumar Dhara
AU  - Shivaji Dey
AU  - Anirvid Sarkar
AU  - Sudipto Haldar
AU  - Glenmer Bathan Tactacan
Y1  - 2021/03/17
PY  - 2021
N1  - https://doi.org/10.11648/j.avs.20210901.13
DO  - 10.11648/j.avs.20210901.13
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 16
EP  - 23
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20210901.13
AB  - A 42-day trial was conducted to assess the effects of a dietary protease on growth performance and ileum population of selected bacteria in broiler chickens fed standard diets and diets deficient in crude protein and digestible amino acids (CP/AA, ~5%) or apparent metabolizable energy (AME, 50 kcal/kg) or both. Reducing CP/AA and AME negatively affected average daily weight gain (ADG, P P P = 0.021, linear effect) of birds fed both standard and nutrient deficient diets but improved FCR (P = 0.0002) was only observed when supplemented to the standard diet. Serum protein concentration was not affected by the level of CP/AA and AME but decreased linearly with the level of protease (P = 0.02). Numbers of Escherichia coli¸ Salmonella spp. and Clostridium perfringens increased (P = 0.0001) with the decrease of dietary CP/AA and AME. In contrast, the numbers of studied bacteria linearly decreased (P = 0.0001) with dietary protease level. Dietary protease was found to decrease the numbers of Clostridium perfringens (P = 0.0001) when supplemented to either low CP/AA or low AME diets. It can be concluded that protease supplementation sustained the harvesting biomass in the experimental birds fed lower nutrient density diets and decreased the numbers of resident bacteria in the hind gut, some of which might be potential pathogens. Hence, the study documented not only the nutrient sparing effects but also the extra-proteinaceous effect in terms of gut health of dietary protease in broiler chickens.
VL  - 9
IS  - 1
ER  -

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Author Information

Mohiuddin Amirul Kabir Chowdhury

Jefo Nutrition Inc., Saint-Hyacinthe, Canada
Amrita Kumar Dhara

Agrivet Consultancy P Ltd., Kolkata, India
Shivaji Dey

Agrivet Consultancy P Ltd., Kolkata, India
Anirvid Sarkar

Agrivet Consultancy P Ltd., Kolkata, India
Sudipto Haldar

Agrivet Consultancy P Ltd., Kolkata, India
Glenmer Bathan Tactacan

Jefo Nutrition Inc., Saint-Hyacinthe, Canada

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APA Style

Mohiuddin Amirul Kabir Chowdhury, Amrita Kumar Dhara, Shivaji Dey, Anirvid Sarkar, Sudipto Haldar, et al. (2021). Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens. Animal and Veterinary Sciences, 9(1), 16-23. https://doi.org/10.11648/j.avs.20210901.13

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ACS Style

Mohiuddin Amirul Kabir Chowdhury; Amrita Kumar Dhara; Shivaji Dey; Anirvid Sarkar; Sudipto Haldar, et al. Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens. Anim. Vet. Sci. 2021, 9(1), 16-23. doi: 10.11648/j.avs.20210901.13

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AMA Style

Mohiuddin Amirul Kabir Chowdhury, Amrita Kumar Dhara, Shivaji Dey, Anirvid Sarkar, Sudipto Haldar, et al. Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens. Anim Vet Sci. 2021;9(1):16-23. doi: 10.11648/j.avs.20210901.13

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@article{10.11648/j.avs.20210901.13,
  author = {Mohiuddin Amirul Kabir Chowdhury and Amrita Kumar Dhara and Shivaji Dey and Anirvid Sarkar and Sudipto Haldar and Glenmer Bathan Tactacan},
  title = {Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens},
  journal = {Animal and Veterinary Sciences},
  volume = {9},
  number = {1},
  pages = {16-23},
  doi = {10.11648/j.avs.20210901.13},
  url = {https://doi.org/10.11648/j.avs.20210901.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20210901.13},
  abstract = {A 42-day trial was conducted to assess the effects of a dietary protease on growth performance and ileum population of selected bacteria in broiler chickens fed standard diets and diets deficient in crude protein and digestible amino acids (CP/AA, ~5%) or apparent metabolizable energy (AME, 50 kcal/kg) or both. Reducing CP/AA and AME negatively affected average daily weight gain (ADG, P P P = 0.021, linear effect) of birds fed both standard and nutrient deficient diets but improved FCR (P = 0.0002) was only observed when supplemented to the standard diet. Serum protein concentration was not affected by the level of CP/AA and AME but decreased linearly with the level of protease (P = 0.02). Numbers of Escherichia coli¸ Salmonella spp. and Clostridium perfringens increased (P = 0.0001) with the decrease of dietary CP/AA and AME. In contrast, the numbers of studied bacteria linearly decreased (P = 0.0001) with dietary protease level. Dietary protease was found to decrease the numbers of Clostridium perfringens (P = 0.0001) when supplemented to either low CP/AA or low AME diets. It can be concluded that protease supplementation sustained the harvesting biomass in the experimental birds fed lower nutrient density diets and decreased the numbers of resident bacteria in the hind gut, some of which might be potential pathogens. Hence, the study documented not only the nutrient sparing effects but also the extra-proteinaceous effect in terms of gut health of dietary protease in broiler chickens.},
 year = {2021}
}

Copy | Download

TY  - JOUR
T1  - Protease Complex Reduces Potentially Pathogenic Microbial Populations in the Ileum While Optimizing Performance of Broiler Chickens
AU  - Mohiuddin Amirul Kabir Chowdhury
AU  - Amrita Kumar Dhara
AU  - Shivaji Dey
AU  - Anirvid Sarkar
AU  - Sudipto Haldar
AU  - Glenmer Bathan Tactacan
Y1  - 2021/03/17
PY  - 2021
N1  - https://doi.org/10.11648/j.avs.20210901.13
DO  - 10.11648/j.avs.20210901.13
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 16
EP  - 23
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20210901.13
AB  - A 42-day trial was conducted to assess the effects of a dietary protease on growth performance and ileum population of selected bacteria in broiler chickens fed standard diets and diets deficient in crude protein and digestible amino acids (CP/AA, ~5%) or apparent metabolizable energy (AME, 50 kcal/kg) or both. Reducing CP/AA and AME negatively affected average daily weight gain (ADG, P P P = 0.021, linear effect) of birds fed both standard and nutrient deficient diets but improved FCR (P = 0.0002) was only observed when supplemented to the standard diet. Serum protein concentration was not affected by the level of CP/AA and AME but decreased linearly with the level of protease (P = 0.02). Numbers of Escherichia coli¸ Salmonella spp. and Clostridium perfringens increased (P = 0.0001) with the decrease of dietary CP/AA and AME. In contrast, the numbers of studied bacteria linearly decreased (P = 0.0001) with dietary protease level. Dietary protease was found to decrease the numbers of Clostridium perfringens (P = 0.0001) when supplemented to either low CP/AA or low AME diets. It can be concluded that protease supplementation sustained the harvesting biomass in the experimental birds fed lower nutrient density diets and decreased the numbers of resident bacteria in the hind gut, some of which might be potential pathogens. Hence, the study documented not only the nutrient sparing effects but also the extra-proteinaceous effect in terms of gut health of dietary protease in broiler chickens.
VL  - 9
IS  - 1
ER  -

Copy | Download