The study was investigated to compare the effectiveness of Folltropin and two products (Fostimon and Menogon) of a recombinant human follicle stimulating hormone (rhFSH) on ovarian super-stimulation and embryo production in dairy Holstein cows. Two different doses for each super-ovulatory product were investigated. The relationship between the levels of Anti-Mullerian Hormone (AMH) in the blood serum before super-stimulation initiation and ovarian response and embryos production were also evaluated. At random stage of the estrus cycle, the donor cows were received intra-vaginal controlled internal drug release device (CIDR) and injected with 100μg of gonadotropin-releasing hormone (GnRH). On fourth day of CIDR insertion, cows were divided according to the product types of superovulatory hormones into three equal main groups. According to hormonal dose, each main group was re-divided into two equally sub-groups; low and high dose. Superovulatory hormones was injected IM as decreasing doses; 40%, 30%, 20% and 10% of the total dose twice daily for 4 days. On day 7, CIDRs were removed and each cow received 2 doses of PGF2α with 12hr interval. Based upon heat detection, donor's cows were inseminated with frozen semen three times 8-10hrs interval and concurrent with 100μg GnRH injection at first insemination. Non-surgical embryos recovery was performed on day's 7-8 post insemination and ovarian super-stimulation was checked using sonography to estimate ovarian response. Recovered embryos were morphologically evaluated as grades. Twelve embryos collected from Menogon donors group were transferred to six recipient's cows. The mean numbers of CL, recovered embryo/ova, fertilized ova and transferable embryos are significantly (P<0.01) higher in Folltropin and Menogon treated cows than Fostimon group. Donor cows treated with high doses of Folltropin and Menogon showed higher means for transferable and freezable embryos. Three recipients (50%) who received embryos appeared pregnant and one of them born calf. Significant positive correlations were reported between AMH on one side and CL, total ovarian response, transferable and freezable embryos on the other side, and cows having AMH≥230ng/L exhibited significant higher means. In conclusion: Menogon can be used as rhFSH for embryos production with transferable quality. AMH can be used as indicator to helpful select cow's candidates as donors. More studies are needed using Fostimon accompanied with different levels of LH.
| Published in |
Animal and Veterinary Sciences (Volume 7, Issue 5)
This article belongs to the Special Issue Application of Advanced Reproductive Technologies for Production Transferable Embryos |
| DOI | 10.11648/j.avs.20190705.11 |
| Page(s) | 104-112 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Dairy Cows, Multiple Ovulation and Embryo Transfer (MOET), Recombinant Human Follicle Stimulating Hormone (rhFSH), Anti-müllerian Hormone (AMH)
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APA Style
Kamel Mohammed, Gamal Darwish, Ragab Dohreig, Ahmed Mahmoud, Zaher Rawash, et al. (2019). Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration. Animal and Veterinary Sciences, 7(5), 104-112. https://doi.org/10.11648/j.avs.20190705.11
ACS Style
Kamel Mohammed; Gamal Darwish; Ragab Dohreig; Ahmed Mahmoud; Zaher Rawash, et al. Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration. Anim. Vet. Sci. 2019, 7(5), 104-112. doi: 10.11648/j.avs.20190705.11
AMA Style
Kamel Mohammed, Gamal Darwish, Ragab Dohreig, Ahmed Mahmoud, Zaher Rawash, et al. Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration. Anim Vet Sci. 2019;7(5):104-112. doi: 10.11648/j.avs.20190705.11
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author = {Kamel Mohammed and Gamal Darwish and Ragab Dohreig and Ahmed Mahmoud and Zaher Rawash and Abdel Khaleik Nassra and Magdy Badr and Ayman Hassan and Sayed Essawy},
title = {Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration},
journal = {Animal and Veterinary Sciences},
volume = {7},
number = {5},
pages = {104-112},
doi = {10.11648/j.avs.20190705.11},
url = {https://doi.org/10.11648/j.avs.20190705.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20190705.11},
abstract = {The study was investigated to compare the effectiveness of Folltropin and two products (Fostimon and Menogon) of a recombinant human follicle stimulating hormone (rhFSH) on ovarian super-stimulation and embryo production in dairy Holstein cows. Two different doses for each super-ovulatory product were investigated. The relationship between the levels of Anti-Mullerian Hormone (AMH) in the blood serum before super-stimulation initiation and ovarian response and embryos production were also evaluated. At random stage of the estrus cycle, the donor cows were received intra-vaginal controlled internal drug release device (CIDR) and injected with 100μg of gonadotropin-releasing hormone (GnRH). On fourth day of CIDR insertion, cows were divided according to the product types of superovulatory hormones into three equal main groups. According to hormonal dose, each main group was re-divided into two equally sub-groups; low and high dose. Superovulatory hormones was injected IM as decreasing doses; 40%, 30%, 20% and 10% of the total dose twice daily for 4 days. On day 7, CIDRs were removed and each cow received 2 doses of PGF2α with 12hr interval. Based upon heat detection, donor's cows were inseminated with frozen semen three times 8-10hrs interval and concurrent with 100μg GnRH injection at first insemination. Non-surgical embryos recovery was performed on day's 7-8 post insemination and ovarian super-stimulation was checked using sonography to estimate ovarian response. Recovered embryos were morphologically evaluated as grades. Twelve embryos collected from Menogon donors group were transferred to six recipient's cows. The mean numbers of CL, recovered embryo/ova, fertilized ova and transferable embryos are significantly (P<0.01) higher in Folltropin and Menogon treated cows than Fostimon group. Donor cows treated with high doses of Folltropin and Menogon showed higher means for transferable and freezable embryos. Three recipients (50%) who received embryos appeared pregnant and one of them born calf. Significant positive correlations were reported between AMH on one side and CL, total ovarian response, transferable and freezable embryos on the other side, and cows having AMH≥230ng/L exhibited significant higher means. In conclusion: Menogon can be used as rhFSH for embryos production with transferable quality. AMH can be used as indicator to helpful select cow's candidates as donors. More studies are needed using Fostimon accompanied with different levels of LH.},
year = {2019}
}
TY - JOUR T1 - Impact of Type and Dose of Recombinant Human Follicle Stimulating Hormone on Embryos Production in Dairy Cows and Their Relationship with AMH Concentration AU - Kamel Mohammed AU - Gamal Darwish AU - Ragab Dohreig AU - Ahmed Mahmoud AU - Zaher Rawash AU - Abdel Khaleik Nassra AU - Magdy Badr AU - Ayman Hassan AU - Sayed Essawy Y1 - 2019/11/13 PY - 2019 N1 - https://doi.org/10.11648/j.avs.20190705.11 DO - 10.11648/j.avs.20190705.11 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 104 EP - 112 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20190705.11 AB - The study was investigated to compare the effectiveness of Folltropin and two products (Fostimon and Menogon) of a recombinant human follicle stimulating hormone (rhFSH) on ovarian super-stimulation and embryo production in dairy Holstein cows. Two different doses for each super-ovulatory product were investigated. The relationship between the levels of Anti-Mullerian Hormone (AMH) in the blood serum before super-stimulation initiation and ovarian response and embryos production were also evaluated. At random stage of the estrus cycle, the donor cows were received intra-vaginal controlled internal drug release device (CIDR) and injected with 100μg of gonadotropin-releasing hormone (GnRH). On fourth day of CIDR insertion, cows were divided according to the product types of superovulatory hormones into three equal main groups. According to hormonal dose, each main group was re-divided into two equally sub-groups; low and high dose. Superovulatory hormones was injected IM as decreasing doses; 40%, 30%, 20% and 10% of the total dose twice daily for 4 days. On day 7, CIDRs were removed and each cow received 2 doses of PGF2α with 12hr interval. Based upon heat detection, donor's cows were inseminated with frozen semen three times 8-10hrs interval and concurrent with 100μg GnRH injection at first insemination. Non-surgical embryos recovery was performed on day's 7-8 post insemination and ovarian super-stimulation was checked using sonography to estimate ovarian response. Recovered embryos were morphologically evaluated as grades. Twelve embryos collected from Menogon donors group were transferred to six recipient's cows. The mean numbers of CL, recovered embryo/ova, fertilized ova and transferable embryos are significantly (P<0.01) higher in Folltropin and Menogon treated cows than Fostimon group. Donor cows treated with high doses of Folltropin and Menogon showed higher means for transferable and freezable embryos. Three recipients (50%) who received embryos appeared pregnant and one of them born calf. Significant positive correlations were reported between AMH on one side and CL, total ovarian response, transferable and freezable embryos on the other side, and cows having AMH≥230ng/L exhibited significant higher means. In conclusion: Menogon can be used as rhFSH for embryos production with transferable quality. AMH can be used as indicator to helpful select cow's candidates as donors. More studies are needed using Fostimon accompanied with different levels of LH. VL - 7 IS - 5 ER -
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