Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks

Rui Liu; Jing Wang; Fugui Li

doi:doi:10.11648/j.avs.20190701.12

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Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks

Rui Liu, Jing Wang, Fugui Li

Published in Animal and Veterinary Sciences (Volume 7, Issue 1)

Received: 10 January 2019 Accepted: 27 February 2019 Published: 20 March 2019

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Abstract

The Hydropericardium-hepatitis Syndrome (HHS) caused by Fowl adenovirus 4 (FAV-4) is a new epidemic disease in recent years and firstly reported in Tianjin, 2016. The isolation, identification and pathogenicity of FAV-4 Tianjin strain were studied in the paper for providing the theoretical basis to the law of occurrence, development and termination. The virus was isolated and identified from dead & sick birds on a chicken farm in Tianjin by chicken embryo inoculation with tissue homogenate supernatant, PCR and sequencing experiment with viral allantoic fluid; And the pathogenicity was proceeded by inoculating the isolate into 100 one-day-old chicks, which divided into group A, B, C (experimental group) and group D (control), the symptoms, mortality and morphological changes were observed daily, meanwhile the virus was detected by PCR to be sure the same as inoculating strain. The results show that the isolate was 665bp in electrophoresis, had homology of 98.3%-97.3% with FAV-4 by sequencing analysis and finally named as FAV-4 TJ1607 strain in GenBank; They also show in Pathogenicity research that the symptoms were appeared on the 3nd day, death on the 4th day and the peak of death on the 5th day and end of the 8th day in group A and B; The mortality was 64% in group A and 72% in group B; Hepatic edema & congestion, large amounts of basophilic intranuclear inclusions, hydropericardium, parechymal myocarditis and pulmonary edema were observed obviously on the 4th day in group A and B; ALL above changes were the same in group C just appeared 3 days later except death trend and mortality for 32%; And FAV-4 TJ1607 was detected in all experimental groups. The measures of prevention and treatment will be recommended in poultry industry accordance with the HHS disease model.

Published in	Animal and Veterinary Sciences (Volume 7, Issue 1)
DOI	10.11648/j.avs.20190701.12
Page(s)	12-17
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Isolation, Identification, FAV-4 TJ1607 Strain, Pathogenicity, HHS Disease Model

References

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[3]	Wang Jing, Zhang Wensheng, Liu Rui, Li Fugui. Avian pericardial effusion-hepatitis syndrome in Tianjin and its virus isolation and identification [J]. Veterinary Medicine of China, 2017, 53(3):16-17.
[4]	Xu Jinghui, Lou Shaosong, Tan Qiaoyan, Huang Jiachao. Methods for the establishment of animal models of hepatic injury and progress in molecular mechanisms. Seventh South China Regional Laboratory Animal Science and Technology Exchange Proceedings.
[5]	He Haijian. 2001. Techniques and inspection points of carcass necropsy. Shandong poultry, 1(4), 16-17.
[6]	Li Quan. 2011. Method of chicken body dissection. Pig industry observation (5), 29-30.
[7]	Zhao J, Zhong Q, Zhao Y. Pathogenicity and complete genome characterization of fowl adenoviruses isolated from chickens associated with inclusion body hepatitis and hydropericardium syndrome in China [J]. PLos One, 2015, 10(7): e0133073.
[8]	Yan Youxiang. 2016. I. Prevalence and control of flock adenovirus infection. Northern Herd (10), 21-21.
[9]	Gai Shuwen. 2016. Diagnosis and treatment of broiler chicken Ankara disease-pericardial effusion hepatitis syndrome. Chinese Livestock and Poultry Breeding, 12(9), 154-154.
[10]	Liu Dianzuo. 2015. Prevention and treatment of pericardial hydrocephalus-hepatitis syndrome. China Animal Health (10), 49-50.
[11]	Mcferran J B, Smyth J A. Avian adenoviruses. [J]. Revue Scientifique Et Technique, 2000, 19(2):589-601.
[12]	Saifuddin M and Wilks C R. Pathogenesis of an acute viral hepatitis: inclusion body hepatitis in the chicken. Archives of Virology, 1991, (1):33-43.
[13]	Saifuddin M and Wilks C R. Reproduction of nclusion body hepatitis in conventionally raised chickens inoculated with a New Zealand isolate of avian adenovirus. New Zealand Veterinary Journal, 1990, (2):62.
[14]	Huang Bing. Expression of avian influenza H5 hemagglutinin avian adenovirus recombinant and its characteristics [D]. Nanjing: Nanjing Agricultural University, 2005.
[15]	Abdul-Aziz T A, Hasan S Y. Hydropericardium syndrome in broiler chickens: its contagious nature and pathology [J], Res Vet Sci, 1995, (59):219-221.
[16]	MACFERRAN J B, CALN EK B W, BERAD H J, MCDOUGALD L R. Group I adenovirus infections [M]. Diseases of poultry. 10th ed. Ames: IA Press, 1997:608-620.

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APA Style

Rui Liu, Jing Wang, Fugui Li. (2019). Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks. Animal and Veterinary Sciences, 7(1), 12-17. https://doi.org/10.11648/j.avs.20190701.12

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ACS Style

Rui Liu; Jing Wang; Fugui Li. Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks. Anim. Vet. Sci. 2019, 7(1), 12-17. doi: 10.11648/j.avs.20190701.12

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AMA Style

Rui Liu, Jing Wang, Fugui Li. Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks. Anim Vet Sci. 2019;7(1):12-17. doi: 10.11648/j.avs.20190701.12

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@article{10.11648/j.avs.20190701.12,
  author = {Rui Liu and Jing Wang and Fugui Li},
  title = {Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks},
  journal = {Animal and Veterinary Sciences},
  volume = {7},
  number = {1},
  pages = {12-17},
  doi = {10.11648/j.avs.20190701.12},
  url = {https://doi.org/10.11648/j.avs.20190701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20190701.12},
  abstract = {The Hydropericardium-hepatitis Syndrome (HHS) caused by Fowl adenovirus 4 (FAV-4) is a new epidemic disease in recent years and firstly reported in Tianjin, 2016. The isolation, identification and pathogenicity of FAV-4 Tianjin strain were studied in the paper for providing the theoretical basis to the law of occurrence, development and termination. The virus was isolated and identified from dead & sick birds on a chicken farm in Tianjin by chicken embryo inoculation with tissue homogenate supernatant, PCR and sequencing experiment with viral allantoic fluid; And the pathogenicity was proceeded by inoculating the isolate into 100 one-day-old chicks, which divided into group A, B, C (experimental group) and group D (control), the symptoms, mortality and morphological changes were observed daily, meanwhile the virus was detected by PCR to be sure the same as inoculating strain. The results show that the isolate was 665bp in electrophoresis, had homology of 98.3%-97.3% with FAV-4 by sequencing analysis and finally named as FAV-4 TJ1607 strain in GenBank; They also show in Pathogenicity research that the symptoms were appeared on the 3nd day, death on the 4th day and the peak of death on the 5th day and end of the 8th day in group A and B; The mortality was 64% in group A and 72% in group B; Hepatic edema & congestion, large amounts of basophilic intranuclear inclusions, hydropericardium, parechymal myocarditis and pulmonary edema were observed obviously on the 4th day in group A and B; ALL above changes were the same in group C just appeared 3 days later except death trend and mortality for 32%; And FAV-4 TJ1607 was detected in all experimental groups. The measures of prevention and treatment will be recommended in poultry industry accordance with the HHS disease model.},
 year = {2019}
}

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TY  - JOUR
T1  - Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks
AU  - Rui Liu
AU  - Jing Wang
AU  - Fugui Li
Y1  - 2019/03/20
PY  - 2019
N1  - https://doi.org/10.11648/j.avs.20190701.12
DO  - 10.11648/j.avs.20190701.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 12
EP  - 17
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20190701.12
AB  - The Hydropericardium-hepatitis Syndrome (HHS) caused by Fowl adenovirus 4 (FAV-4) is a new epidemic disease in recent years and firstly reported in Tianjin, 2016. The isolation, identification and pathogenicity of FAV-4 Tianjin strain were studied in the paper for providing the theoretical basis to the law of occurrence, development and termination. The virus was isolated and identified from dead & sick birds on a chicken farm in Tianjin by chicken embryo inoculation with tissue homogenate supernatant, PCR and sequencing experiment with viral allantoic fluid; And the pathogenicity was proceeded by inoculating the isolate into 100 one-day-old chicks, which divided into group A, B, C (experimental group) and group D (control), the symptoms, mortality and morphological changes were observed daily, meanwhile the virus was detected by PCR to be sure the same as inoculating strain. The results show that the isolate was 665bp in electrophoresis, had homology of 98.3%-97.3% with FAV-4 by sequencing analysis and finally named as FAV-4 TJ1607 strain in GenBank; They also show in Pathogenicity research that the symptoms were appeared on the 3nd day, death on the 4th day and the peak of death on the 5th day and end of the 8th day in group A and B; The mortality was 64% in group A and 72% in group B; Hepatic edema & congestion, large amounts of basophilic intranuclear inclusions, hydropericardium, parechymal myocarditis and pulmonary edema were observed obviously on the 4th day in group A and B; ALL above changes were the same in group C just appeared 3 days later except death trend and mortality for 32%; And FAV-4 TJ1607 was detected in all experimental groups. The measures of prevention and treatment will be recommended in poultry industry accordance with the HHS disease model.
VL  - 7
IS  - 1
ER  -

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Author Information

Rui Liu

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China
Jing Wang

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China
Fugui Li

College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China

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APA Style

Rui Liu, Jing Wang, Fugui Li. (2019). Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks. Animal and Veterinary Sciences, 7(1), 12-17. https://doi.org/10.11648/j.avs.20190701.12

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ACS Style

Rui Liu; Jing Wang; Fugui Li. Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks. Anim. Vet. Sci. 2019, 7(1), 12-17. doi: 10.11648/j.avs.20190701.12

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AMA Style

Rui Liu, Jing Wang, Fugui Li. Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks. Anim Vet Sci. 2019;7(1):12-17. doi: 10.11648/j.avs.20190701.12

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@article{10.11648/j.avs.20190701.12,
  author = {Rui Liu and Jing Wang and Fugui Li},
  title = {Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks},
  journal = {Animal and Veterinary Sciences},
  volume = {7},
  number = {1},
  pages = {12-17},
  doi = {10.11648/j.avs.20190701.12},
  url = {https://doi.org/10.11648/j.avs.20190701.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20190701.12},
  abstract = {The Hydropericardium-hepatitis Syndrome (HHS) caused by Fowl adenovirus 4 (FAV-4) is a new epidemic disease in recent years and firstly reported in Tianjin, 2016. The isolation, identification and pathogenicity of FAV-4 Tianjin strain were studied in the paper for providing the theoretical basis to the law of occurrence, development and termination. The virus was isolated and identified from dead & sick birds on a chicken farm in Tianjin by chicken embryo inoculation with tissue homogenate supernatant, PCR and sequencing experiment with viral allantoic fluid; And the pathogenicity was proceeded by inoculating the isolate into 100 one-day-old chicks, which divided into group A, B, C (experimental group) and group D (control), the symptoms, mortality and morphological changes were observed daily, meanwhile the virus was detected by PCR to be sure the same as inoculating strain. The results show that the isolate was 665bp in electrophoresis, had homology of 98.3%-97.3% with FAV-4 by sequencing analysis and finally named as FAV-4 TJ1607 strain in GenBank; They also show in Pathogenicity research that the symptoms were appeared on the 3nd day, death on the 4th day and the peak of death on the 5th day and end of the 8th day in group A and B; The mortality was 64% in group A and 72% in group B; Hepatic edema & congestion, large amounts of basophilic intranuclear inclusions, hydropericardium, parechymal myocarditis and pulmonary edema were observed obviously on the 4th day in group A and B; ALL above changes were the same in group C just appeared 3 days later except death trend and mortality for 32%; And FAV-4 TJ1607 was detected in all experimental groups. The measures of prevention and treatment will be recommended in poultry industry accordance with the HHS disease model.},
 year = {2019}
}

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TY  - JOUR
T1  - Isolation & Identification of FAV-4 Tianjin Strain and Pathogenicity to Chicks
AU  - Rui Liu
AU  - Jing Wang
AU  - Fugui Li
Y1  - 2019/03/20
PY  - 2019
N1  - https://doi.org/10.11648/j.avs.20190701.12
DO  - 10.11648/j.avs.20190701.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 12
EP  - 17
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20190701.12
AB  - The Hydropericardium-hepatitis Syndrome (HHS) caused by Fowl adenovirus 4 (FAV-4) is a new epidemic disease in recent years and firstly reported in Tianjin, 2016. The isolation, identification and pathogenicity of FAV-4 Tianjin strain were studied in the paper for providing the theoretical basis to the law of occurrence, development and termination. The virus was isolated and identified from dead & sick birds on a chicken farm in Tianjin by chicken embryo inoculation with tissue homogenate supernatant, PCR and sequencing experiment with viral allantoic fluid; And the pathogenicity was proceeded by inoculating the isolate into 100 one-day-old chicks, which divided into group A, B, C (experimental group) and group D (control), the symptoms, mortality and morphological changes were observed daily, meanwhile the virus was detected by PCR to be sure the same as inoculating strain. The results show that the isolate was 665bp in electrophoresis, had homology of 98.3%-97.3% with FAV-4 by sequencing analysis and finally named as FAV-4 TJ1607 strain in GenBank; They also show in Pathogenicity research that the symptoms were appeared on the 3nd day, death on the 4th day and the peak of death on the 5th day and end of the 8th day in group A and B; The mortality was 64% in group A and 72% in group B; Hepatic edema & congestion, large amounts of basophilic intranuclear inclusions, hydropericardium, parechymal myocarditis and pulmonary edema were observed obviously on the 4th day in group A and B; ALL above changes were the same in group C just appeared 3 days later except death trend and mortality for 32%; And FAV-4 TJ1607 was detected in all experimental groups. The measures of prevention and treatment will be recommended in poultry industry accordance with the HHS disease model.
VL  - 7
IS  - 1
ER  -

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