Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (<i>Acipenser schrenckii</i>)

Xiujuan Zhang; Jinge Ma; Linmiao Li; Haiying Jiang; Jinping Chen

doi:doi:10.11648/j.avs.20180604.11

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Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii)

Xiujuan Zhang, Jinge Ma, Linmiao Li, Haiying Jiang, Jinping Chen

Published in Animal and Veterinary Sciences (Volume 6, Issue 4)

Received: 4 August 2018 Accepted: 4 September 2018 Published: 13 October 2018

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Abstract

Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.

Published in	Animal and Veterinary Sciences (Volume 6, Issue 4)
DOI	10.11648/j.avs.20180604.11
Page(s)	51-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Acipenser schrenckii, Cryoprotectant, Sperm Cryopreservation, Embryo Development

References

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[2]	Wei, Q.W., Zou, Y., Li, P., and Li, L. 2011. Sturgeon aquaculture in China: progress, strategies and prospects assessed on the basis of nation-wide surveys (2007-2009). Journal of Applied Ichthyology, 27: 162-168.
[3]	Li, D., Liu, Z., and Xie, C. 2012. Effect of stocking density on growth and serum concentrations of thyroid hormones and cortisol in Amur sturgeon, Acipenser schrenckii. Fish Physiology & Biochemistry, 38: 511-520.
[4]	Arakawa, T., Carpenter, J. F., Kita, Y. A., and Crowe, J. H. 1990. The basis for toxicity of certain cryoprotectants: A hypothesis. Cryobiology, 27:401-415.
[5]	Jun, L. I., Liu, Q., and Zhang, S. 2006. Evaluation of the damage in fish spermatozoa cryopreservation. Chinese Journal of Oceanology and Limnology, 24: 370-377.
[6]	Glogowski, J., Kolman, R., Szczepkowski, M., Horváth, Á., Urbányi, B., Sieczyński, P., et al. 2002. Fertilization rate of Siberian sturgeon (Acipenser baeri, Brandt) milt cryopreserved with methanol. Aquaculture, 211: 367-373.
[7]	Dzyuba, B., Boryshpolets, S., Shaliutina, A., Rodina, M., Yamaner, G., Gela, D., et al. 2012. Spermatozoa motility, cryoresistance, and fertilizing ability in sterlet Acipenser ruthenus during sequential stripping. Aquaculture, 356-357: 272-278.
[8]	Aramli, M. S., Golshahi, K., Nazari, R. M., and Aramli, S. 2015. Effect of freezing rate on motility, adenosine triphosphate content and fertilizability in beluga sturgeon (Huso huso) spermatozoa. Cryobiology, 70: 170-174.
[9]	Aramli, M. S., Golshahi, K., Nazari, R. M., Golpour, A., Aramli, S. 2016. Influence of Glutamine Supplementation on Motility and Fertilization Success of Frozen-Thawed Persian Sturgeon (Acipenser persicus) Sperm. Reprod. Domest. Anim., 51: 474-477.
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[12]	Liu, Q., Li, J., Zhang, S., Ding, F., Xu, X., Xiao, Z., et al. 2006. An Efficient Methodology for Cryopreservation of Spermatozoa of Red Seabream, Pagrus major, with 2-mL Cryovials. Journal of the World Aquaculture Society, 37: 289–297.
[13]	Klimowicz, M. D., Nizanski, W., Batkowski, F., and Savic, M.A. 2008. The comparison of assessment of pigeon semen motility and sperm concentration by conventional methods and the CASA system (HTM IVOS). Theriogenology, 70: 77.
[14]	Dreanno C., Suquet M., Quemener L., Cosson J., Fierville F., Normant Y., and Billard. 1997. Cryopreservation of turbot (Scophthalmus maximus) spermatozoa. Theriogenology, 48: 589.
[15]	Horvath, A., Wayman, W. R., Urbanyi, B., Ware, K. M., Dean, J.C., and Tiersch, T. R. 2005. The relationship of the cryoprotectants methanol and dimethyl sulfoxide and hyperosmotic extenders on sperm cryopreservation of two North-American sturgeon species. Aquaculture, 247: 243-251.
[16]	Fabbrocini, A., Lavadera, S.L., Rispoli, S., Sansone, G. 2002. Cryopreservation of seabream (Sparus aurata) spermatozoa. Cryobiology, 40: 46.
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APA Style

Xiujuan Zhang, Jinge Ma, Linmiao Li, Haiying Jiang, Jinping Chen. (2018). Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii). Animal and Veterinary Sciences, 6(4), 51-57. https://doi.org/10.11648/j.avs.20180604.11

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ACS Style

Xiujuan Zhang; Jinge Ma; Linmiao Li; Haiying Jiang; Jinping Chen. Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii). Anim. Vet. Sci. 2018, 6(4), 51-57. doi: 10.11648/j.avs.20180604.11

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AMA Style

Xiujuan Zhang, Jinge Ma, Linmiao Li, Haiying Jiang, Jinping Chen. Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii). Anim Vet Sci. 2018;6(4):51-57. doi: 10.11648/j.avs.20180604.11

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@article{10.11648/j.avs.20180604.11,
  author = {Xiujuan Zhang and Jinge Ma and Linmiao Li and Haiying Jiang and Jinping Chen},
  title = {Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii)},
  journal = {Animal and Veterinary Sciences},
  volume = {6},
  number = {4},
  pages = {51-57},
  doi = {10.11648/j.avs.20180604.11},
  url = {https://doi.org/10.11648/j.avs.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180604.11},
  abstract = {Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.},
 year = {2018}
}

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TY  - JOUR
T1  - Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii)
AU  - Xiujuan Zhang
AU  - Jinge Ma
AU  - Linmiao Li
AU  - Haiying Jiang
AU  - Jinping Chen
Y1  - 2018/10/13
PY  - 2018
N1  - https://doi.org/10.11648/j.avs.20180604.11
DO  - 10.11648/j.avs.20180604.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 51
EP  - 57
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20180604.11
AB  - Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.
VL  - 6
IS  - 4
ER  -

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Author Information

Xiujuan Zhang

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Jinge Ma

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Linmiao Li

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Haiying Jiang

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China
Jinping Chen

Guangdong Key Laboratory of Animal Conservation and Resource Utilization, Guangdong Public Laboratory of Wild Animal Conservation and Utilization, Guangdong Institute of Applied Biological Resources, Guangzhou, China

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APA Style

Xiujuan Zhang, Jinge Ma, Linmiao Li, Haiying Jiang, Jinping Chen. (2018). Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii). Animal and Veterinary Sciences, 6(4), 51-57. https://doi.org/10.11648/j.avs.20180604.11

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ACS Style

Xiujuan Zhang; Jinge Ma; Linmiao Li; Haiying Jiang; Jinping Chen. Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii). Anim. Vet. Sci. 2018, 6(4), 51-57. doi: 10.11648/j.avs.20180604.11

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AMA Style

Xiujuan Zhang, Jinge Ma, Linmiao Li, Haiying Jiang, Jinping Chen. Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii). Anim Vet Sci. 2018;6(4):51-57. doi: 10.11648/j.avs.20180604.11

Copy | Download

@article{10.11648/j.avs.20180604.11,
  author = {Xiujuan Zhang and Jinge Ma and Linmiao Li and Haiying Jiang and Jinping Chen},
  title = {Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii)},
  journal = {Animal and Veterinary Sciences},
  volume = {6},
  number = {4},
  pages = {51-57},
  doi = {10.11648/j.avs.20180604.11},
  url = {https://doi.org/10.11648/j.avs.20180604.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20180604.11},
  abstract = {Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.},
 year = {2018}
}

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TY  - JOUR
T1  - Effects of Different Cytoprotectants Combination on Sperm Survival, Fertility and Embryo Development in Amur Sturgeon (Acipenser schrenckii)
AU  - Xiujuan Zhang
AU  - Jinge Ma
AU  - Linmiao Li
AU  - Haiying Jiang
AU  - Jinping Chen
Y1  - 2018/10/13
PY  - 2018
N1  - https://doi.org/10.11648/j.avs.20180604.11
DO  - 10.11648/j.avs.20180604.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 51
EP  - 57
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20180604.11
AB  - Development of artificial propagation technology is becoming increasingly important in sturgeon aquaculture whether species recovery efforts or commercial production. The cryopreservation technique of high-quality semen collected during the spawning season could extremely improve reproductive efficiency for year-round availability, especially for off-season use. However, the cryopreservation technique of sperm in Amur sturgeon (Acipenser schrenckii) has not been effectively developed. In the present study, we firstly tested the cryopreservations combination effects of three cryoprotectants, including methanol, dimethyl sulfoxide (DMSO), and propylene glycol (PG) and fresh yolk (Y) addition on sperm motility, fertilization and embryo development in Amur sturgeon. The results indicated that sperm motility was still more than 60% in the MT group but that of the control group was sharply decreased to 4.5% after 72 h of in vitro storage (4°C). The post-thawed sperm motility analysis showed that there was no significant difference between the MT+DMSO group and MT group, but the fertility rate in the MT group was significantly higher with a value of 42.30±2.57(%) than any other experimental groups, including the MT+DMSO group (P<0.05). Meanwhile, we also found that there was no significantly positive effect on post-thawed sperm motility with Y addition. Interestingly, although the results showed that the MT+DMSO group and MT group had similar effects on the post-thawed sperm motility, the MT+DMSO group had higher hatching rate compared to any other tested groups, including the MT group. Meanwhile, we also found that PG as cryoprotectant was unsuitable for sperm cryopreservation of Amur sturgeon. In conclusion, our results provides invaluable basis in further studies for the optimization technology of artificial propagation in Amur sturgeon.
VL  - 6
IS  - 4
ER  -

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