The Prevalence of <i>Melissococcus plutonius</i> by Polymerase Chain Reaction in Kurdistan apiaries, Iran

Mohammad Khezri; Mojtaba Moharami; Hussein Modirrousta; Zahra Salahi; Babak Rokhzad

doi:doi:10.11648/j.avs.20160406.12

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The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran

Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad

Published in Animal and Veterinary Sciences (Volume 4, Issue 6)

Received: 12 July 2016 Accepted: 26 July 2016 Published: 14 December 2016

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Abstract

European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.

Published in	Animal and Veterinary Sciences (Volume 4, Issue 6)
DOI	10.11648/j.avs.20160406.12
Page(s)	93-96
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

M. plutonius, European foulbrood, PCR, Kurdistan apiaries

References

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APA Style

Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad. (2016). The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Animal and Veterinary Sciences, 4(6), 93-96. https://doi.org/10.11648/j.avs.20160406.12

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ACS Style

Mohammad Khezri; Mojtaba Moharami; Hussein Modirrousta; Zahra Salahi; Babak Rokhzad. The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Anim. Vet. Sci. 2016, 4(6), 93-96. doi: 10.11648/j.avs.20160406.12

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AMA Style

Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad. The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Anim Vet Sci. 2016;4(6):93-96. doi: 10.11648/j.avs.20160406.12

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@article{10.11648/j.avs.20160406.12,
  author = {Mohammad Khezri and Mojtaba Moharami and Hussein Modirrousta and Zahra Salahi and Babak Rokhzad},
  title = {The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {6},
  pages = {93-96},
  doi = {10.11648/j.avs.20160406.12},
  url = {https://doi.org/10.11648/j.avs.20160406.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160406.12},
  abstract = {European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.},
 year = {2016}
}

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TY  - JOUR
T1  - The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran
AU  - Mohammad Khezri
AU  - Mojtaba Moharami
AU  - Hussein Modirrousta
AU  - Zahra Salahi
AU  - Babak Rokhzad
Y1  - 2016/12/14
PY  - 2016
N1  - https://doi.org/10.11648/j.avs.20160406.12
DO  - 10.11648/j.avs.20160406.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 93
EP  - 96
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20160406.12
AB  - European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.
VL  - 4
IS  - 6
ER  -

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Author Information

Mohammad Khezri

Veterinary Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran
Mojtaba Moharami

Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
Hussein Modirrousta

Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
Zahra Salahi

Department of Honeybee, Silkworm and Wildlife Research Disease, Razi Vaccine and Serum Research Institute, AREEO, Karaj, Iran
Babak Rokhzad

Veterinary Research Department, Agricultural and Natural Resources Research and Education Center, AREEO, Sanandaj, Iran

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Plain Text BibTeX RIS

APA Style

Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad. (2016). The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Animal and Veterinary Sciences, 4(6), 93-96. https://doi.org/10.11648/j.avs.20160406.12

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ACS Style

Mohammad Khezri; Mojtaba Moharami; Hussein Modirrousta; Zahra Salahi; Babak Rokhzad. The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Anim. Vet. Sci. 2016, 4(6), 93-96. doi: 10.11648/j.avs.20160406.12

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AMA Style

Mohammad Khezri, Mojtaba Moharami, Hussein Modirrousta, Zahra Salahi, Babak Rokhzad. The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran. Anim Vet Sci. 2016;4(6):93-96. doi: 10.11648/j.avs.20160406.12

Copy | Download

@article{10.11648/j.avs.20160406.12,
  author = {Mohammad Khezri and Mojtaba Moharami and Hussein Modirrousta and Zahra Salahi and Babak Rokhzad},
  title = {The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {6},
  pages = {93-96},
  doi = {10.11648/j.avs.20160406.12},
  url = {https://doi.org/10.11648/j.avs.20160406.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160406.12},
  abstract = {European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - The Prevalence of Melissococcus plutonius by Polymerase Chain Reaction in Kurdistan apiaries, Iran
AU  - Mohammad Khezri
AU  - Mojtaba Moharami
AU  - Hussein Modirrousta
AU  - Zahra Salahi
AU  - Babak Rokhzad
Y1  - 2016/12/14
PY  - 2016
N1  - https://doi.org/10.11648/j.avs.20160406.12
DO  - 10.11648/j.avs.20160406.12
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 93
EP  - 96
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20160406.12
AB  - European foulbrood (EFB) is caused by bacterial agents Melissococcus plutonius and is considered of major importance because it can be spread through many bee products including honey, which is internationally widely traded. A polymerase chain reaction (PCR) molecular diagnosis survey for honey bee pathogen EFB was conducted on European honey bee (EHB), Apis mellifera, population sampled from Kurdistan apiaries (n=100). For multiplex PCR amplification of partial 16S rRNA (= SSU rRNA) gene fragments (486 bp), was conducted on 100 samples. Results showed that of 100 samples only 4 samples (4%) were positive for M. plutonius in Kurdistan apiaries. This is the first study to document the occurrence of EFB by PCR assay in Kurdistan apiaries. It seems that surveys are required to characterize the distribution and prevalence of M. plutonius in different regions of Iran.
VL  - 4
IS  - 6
ER  -

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