The objective of this study was to characterize the immunohistochemical localization of plasma membrane connexin 43 in the rumen of sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). Using immunohistochemical technique the present study confirmed the existence of plasma membrane connexin 43 in the sheep rumen epithelium. Plasma membrane connexin 43 immunostaining was most intense at the stratum basale and stratum spinosum (suprabasal layer) and decreased iron intensity through stratum spinosum (superficial layers) to stratum granulosum. Meanwhile, stratum corneum was negative. The reaction around the cells gave a syncitial appearance with more apical-immunostaining concentration. Moreover, the present study confirmed a significant effect of concentrate diet on the immunoreactivity of plasma membrane connexin 43 in the rumen of sheep. A very strong degree of antibody reaction was seen in 4 to 12 weeks concentrate-fed groups.
| Published in | Animal and Veterinary Sciences (Volume 4, Issue 1) |
| DOI | 10.11648/j.avs.20160401.13 |
| Page(s) | 11-14 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
Sheep, Rumen, Connexin 43, Feed, Immunohistochemistry
| [1] | Benedetti, E. L. and Emmelot, P. (1965) Electron microscopic observations on negatively stained plasma membranes isolated from rat liver. J. Cell Biol. 26: 299–305. |
| [2] | Gäbel, G.; Martens, H.; Suendermann, M. and Galfi, P. (1987) The effect of diet, intraruminal pH and osmolarity on sodium, chloride and magnesium absorption from the temporarily isolated and washed reticulo-rumen of sheep. Q. J. Exp. Physiol. 72: 501-511. |
| [3] | Graham, C. and Simmons, N. L. (2005) Functional organization of the bovine rumen epithelium. Am. J. Physiol. Regul. Integr. Comp Physiol. 288: 173-181. |
| [4] | Henderson, D.; Eibl, H. and Weber, K. (1979) Structure and biochemistry of mouse hepatic gap junctions. J. Mol. Biol. 132: 193–218 |
| [5] | Hertzberg, E. L. and Gilula, N. B. (1979) Isolation and characterization of gap junctions from rat liver. J. Biol. Chem. 254: 2138–2147 |
| [6] | Kistler, J.; Kirkland, B. and Bullivant, S. (1985) Identification of a 70,000-D protein in lens membrane junctional domains. J. Cell Biol. 101: 28–35. |
| [7] | Mills, J. W.; Ernst, S. A. and DiBona, D. R. (1977) Localization of Na-pump sites in frog skin. J. Cell Biol. 73: 88-110. |
| [8] | Revel, J. P. and Karnovsky, M. J. (1967) Hexagonal array of subunits in intercellular junctions of the mouse heart and liver. J. Cell Biol. 33: C7–C12. |
| [9] | Romeis, B. (1989) Mikroskopische Technik.Verlag Urban und Schwarzenberg, München, Wien und Baltimore, 17. Auflage. |
| [10] | Saez, J. C.; Berthoud, V. M.; Branes, M. C.; Martinez, A. G. and Beyer, E. C. (2003) Plasma membrane channels formed by connexins: Their regulation and functions. Physiol. Rev. 83: 1359-1400. |
| [11] | Salomon, D., Masgrau, E., Vischer, S., Ullrich, S., Dupont, E., Sappino, P., Saurat, J. H. and Meda, P. (1994) Topography of mammalian connexins in human skin. J. Invest. Dermatol. 103: 240-247. |
| [12] | Shahin, S. H. and Blankemeyer, J. T. (1989) Demonstration of gap junctions in frog skin epithelium. Am. J. Physiol. 257: C 658-664. |
| [13] | Tada, J. and Hashimoto, K. (1997) Ultrastructural localization of gap junction protein connexin 43 in normal human skin, basal cell carcinoma and squamous cell carcinoma. J. Cutan. Pathol. 24: 628-635. |
| [14] | White, T. W. (2003) Non redundant gap-junction functions. News Physiol. Sci. 18: 95-99. |
APA Style
Rasha S. Ahmed, Holger Martens, Christoph Muelling. (2016). Diet-Dependent Immunohistochemical Evaluation of Connexin 43 in the Sheep Rumen. Animal and Veterinary Sciences, 4(1), 11-14. https://doi.org/10.11648/j.avs.20160401.13
ACS Style
Rasha S. Ahmed; Holger Martens; Christoph Muelling. Diet-Dependent Immunohistochemical Evaluation of Connexin 43 in the Sheep Rumen. Anim. Vet. Sci. 2016, 4(1), 11-14. doi: 10.11648/j.avs.20160401.13
AMA Style
Rasha S. Ahmed, Holger Martens, Christoph Muelling. Diet-Dependent Immunohistochemical Evaluation of Connexin 43 in the Sheep Rumen. Anim Vet Sci. 2016;4(1):11-14. doi: 10.11648/j.avs.20160401.13
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Download@article{10.11648/j.avs.20160401.13,
author = {Rasha S. Ahmed and Holger Martens and Christoph Muelling},
title = {Diet-Dependent Immunohistochemical Evaluation of Connexin 43 in the Sheep Rumen},
journal = {Animal and Veterinary Sciences},
volume = {4},
number = {1},
pages = {11-14},
doi = {10.11648/j.avs.20160401.13},
url = {https://doi.org/10.11648/j.avs.20160401.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160401.13},
abstract = {The objective of this study was to characterize the immunohistochemical localization of plasma membrane connexin 43 in the rumen of sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). Using immunohistochemical technique the present study confirmed the existence of plasma membrane connexin 43 in the sheep rumen epithelium. Plasma membrane connexin 43 immunostaining was most intense at the stratum basale and stratum spinosum (suprabasal layer) and decreased iron intensity through stratum spinosum (superficial layers) to stratum granulosum. Meanwhile, stratum corneum was negative. The reaction around the cells gave a syncitial appearance with more apical-immunostaining concentration. Moreover, the present study confirmed a significant effect of concentrate diet on the immunoreactivity of plasma membrane connexin 43 in the rumen of sheep. A very strong degree of antibody reaction was seen in 4 to 12 weeks concentrate-fed groups.},
year = {2016}
}
TY - JOUR T1 - Diet-Dependent Immunohistochemical Evaluation of Connexin 43 in the Sheep Rumen AU - Rasha S. Ahmed AU - Holger Martens AU - Christoph Muelling Y1 - 2016/02/26 PY - 2016 N1 - https://doi.org/10.11648/j.avs.20160401.13 DO - 10.11648/j.avs.20160401.13 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 11 EP - 14 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20160401.13 AB - The objective of this study was to characterize the immunohistochemical localization of plasma membrane connexin 43 in the rumen of sheep after changing the diet from hay (ad libitum) to a mixed hay/concentrate diet. A total of 24 sheep were fed mixed hay/concentrate for different periods ranging from 0 weeks (control; hay ad libitum) to 12 weeks (1-1.5 kg hay plus 780 g concentrate per day in two equal portions). Using immunohistochemical technique the present study confirmed the existence of plasma membrane connexin 43 in the sheep rumen epithelium. Plasma membrane connexin 43 immunostaining was most intense at the stratum basale and stratum spinosum (suprabasal layer) and decreased iron intensity through stratum spinosum (superficial layers) to stratum granulosum. Meanwhile, stratum corneum was negative. The reaction around the cells gave a syncitial appearance with more apical-immunostaining concentration. Moreover, the present study confirmed a significant effect of concentrate diet on the immunoreactivity of plasma membrane connexin 43 in the rumen of sheep. A very strong degree of antibody reaction was seen in 4 to 12 weeks concentrate-fed groups. VL - 4 IS - 1 ER -
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