Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey

Volkan Yilmaz

doi:doi:10.11648/j.avs.20160401.11

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Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey

Volkan Yilmaz

Published in Animal and Veterinary Sciences (Volume 4, Issue 1)

Received: 8 December 2015 Accepted: 16 December 2015 Published: 30 December 2015

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Abstract

Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.

Published in	Animal and Veterinary Sciences (Volume 4, Issue 1)
DOI	10.11648/j.avs.20160401.11
Page(s)	1-4
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Calves, Diarrhea, ELISA, RT-PCR

References

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APA Style

Volkan Yilmaz. (2015). Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Animal and Veterinary Sciences, 4(1), 1-4. https://doi.org/10.11648/j.avs.20160401.11

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ACS Style

Volkan Yilmaz. Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Anim. Vet. Sci. 2015, 4(1), 1-4. doi: 10.11648/j.avs.20160401.11

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AMA Style

Volkan Yilmaz. Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Anim Vet Sci. 2015;4(1):1-4. doi: 10.11648/j.avs.20160401.11

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@article{10.11648/j.avs.20160401.11,
  author = {Volkan Yilmaz},
  title = {Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {1},
  pages = {1-4},
  doi = {10.11648/j.avs.20160401.11},
  url = {https://doi.org/10.11648/j.avs.20160401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160401.11},
  abstract = {Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.},
 year = {2015}
}

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TY  - JOUR
T1  - Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey
AU  - Volkan Yilmaz
Y1  - 2015/12/30
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20160401.11
DO  - 10.11648/j.avs.20160401.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 1
EP  - 4
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20160401.11
AB  - Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.
VL  - 4
IS  - 1
ER  -

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Author Information

Volkan Yilmaz

Department of Virology, Faculty of Veterinary Medicine, University of Kafkas, Kars, Turkey

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APA Style

Volkan Yilmaz. (2015). Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Animal and Veterinary Sciences, 4(1), 1-4. https://doi.org/10.11648/j.avs.20160401.11

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ACS Style

Volkan Yilmaz. Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Anim. Vet. Sci. 2015, 4(1), 1-4. doi: 10.11648/j.avs.20160401.11

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AMA Style

Volkan Yilmaz. Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey. Anim Vet Sci. 2015;4(1):1-4. doi: 10.11648/j.avs.20160401.11

Copy | Download

@article{10.11648/j.avs.20160401.11,
  author = {Volkan Yilmaz},
  title = {Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey},
  journal = {Animal and Veterinary Sciences},
  volume = {4},
  number = {1},
  pages = {1-4},
  doi = {10.11648/j.avs.20160401.11},
  url = {https://doi.org/10.11648/j.avs.20160401.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20160401.11},
  abstract = {Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Investigation of Rotavirus Infection in Calves with Diarrhea in Northeast Turkey
AU  - Volkan Yilmaz
Y1  - 2015/12/30
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20160401.11
DO  - 10.11648/j.avs.20160401.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 1
EP  - 4
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20160401.11
AB  - Rotavirus is the most common cause of severe diarrhea in newborn calves. In this study, it was aimed to investigate frequency of Rotavirus infections in calves in Kars region in Northeast Turkey by feces sampling 112 diarrheic calves of 0-6 months old. Feces samples were tested for Rotavirus antigen by Enzyme Linked Immunosorbent Assay (ELISA). Of the 112 feces samples examined by ELISA 10 samples were identified as positive (8.92%). At the molecular stage, reverse transcription polymerase chain reaction (RT-PCR) technique was used to determine the presence of Rotavirus nucleic acid by using specific generic primers for VP6 genetic regions in feces samples. The 10 samples that were rotavirus antigen positive by ELISA were also positive on RT-PCR technique. The proportion of positivity in feces samples suggested that rotavirus infections are common in the Kars region in Northeast Turkey.
VL  - 4
IS  - 1
ER  -

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