Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria

Mai-Siyama Bukar Ibrahim; Okon Kenneth Okwong; Adamu Nuhu Bala; Mohammed Abdulrahman; Hamidu Ibrahim; Hambali Idris Umar; Jajere Saleh Mohammed

doi:doi:10.11648/j.avs.20150305.11

Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria

Mai-Siyama Bukar Ibrahim, Okon Kenneth Okwong, Adamu Nuhu Bala, Mohammed Abdulrahman, Hamidu Ibrahim, Hambali Idris Umar, Jajere Saleh Mohammed

Published in Animal and Veterinary Sciences (Volume 3, Issue 5)

Received: 11 May 2015 Accepted: 22 May 2015 Published: 21 July 2015

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Abstract

Coagulase negative staphylococci (CNS) are primary cause of mastitis in ruminant animal, and can either be transmitted by contagious or by environmental means. Therefore, we speculate whether there are similarity in the species distribution of CNS colonsing the anterior nare and milk of ruminant animals that can serve as source of infection. The study examined the species of CNS from the anterior nare of ruminant animals and contact persons. A total of 75 CNS isolates were analysed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), mecA gene detection by PCR assay and antibiotic susceptibility testing according to CSLI guidelines. Overall, four CNS spp were identified, S.sciuri predominate 71 (96%) and were recovered from all samples analysed, while one specie each (1.3%) of S.lentus, S.condementii and S.xylosus (1.3%) respectively. No mecA gene was detected in the CNS spp identified. The CNS spp demonstrated low to moderate resistance pattern (15-30%) to all antibiotics tested. In conclusion, the study provided a baseline epidemiological information on CNS spp colonizing the ruminants ruminant animal and contact persons. In addition, non-detection of mecA gene and high sensitivity pattern demonstrated by the CNS species.

Published in	Animal and Veterinary Sciences (Volume 3, Issue 5)
DOI	10.11648/j.avs.20150305.11
Page(s)	128-131
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Coagulase Negative Staphylococci, Species, Ruminant Animals, Maiduguri

References

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[2]	Bansal BK, Gupta HK, Shafi TA, Sharma S. ’’Comparative antibiogram of coagulase negative staphylococci(CNS) associated with subclinical and clinical mastitis in dairy cow.Vet World, 3, 421-425.2015
[3]	CSLI.’’Perfomance standard for antimicrobial Disk susceptibility Test.Sixteeth International Supplement Clinical and Laboratory Standard Institute’’, Wayne, PA,USA, 2006
[4]	Davidson TJ, Dohoo IR, Donald AW Hariharan H, Collins K, ‘’A cohort study of coagulase negative staphylococcal mastitis in selected dairy herds in Prince Edward Island.’’. Can,J,Vet Res.56,275-280. 1992
[5]	Denis M, Lacy-Hulbert SJ, Buddle BM, WilliansonJH, Wedlock DN,’’Streptococcus uberis-specific-T- cells are present in mammary gland secretion of cow and can be associated to kill S.ubertis. Vet Res Community, 35, 145-150, 2011
[6]	Dubois D, Leyssene D, Chacomac JP, Rostrzewa M,Schmit PO, Talon R, Bonnet R,Dalmas J.’’Idenification of a variety of staphylococcal species by matrix assosciated desorption ionization- time of flight mass spectrometry’’. J.Clin Microbiol. 48,941-945, 2010
[7]	Huber H, Ziegler D, Pfluger V, Vogel G V, Zwelfe C, Stephen R. ‘’Prevalence and characterisitic of methicillin-resistant coagulase-negative staphylococci from livestock, chicken, carcasses, milk, tank milk, minced meat and contact persons.’’.BMC Veterinay Research, 79; 2011.
[8]	Karmano J,Shimizu A, Saitoh Y, Yagi M, Saito T, Okamato R.’’Isolation of methicillin-resistant coagulase negative staphylococci from chicken’’.J.Clin Micribiol.34; 2072-2077, 1996.
[9]	Mai-siyama IB, Okon KO,Adamu NB, Askira UM, Isyaka TM, Adamu SG, Mohammed A. ‘’ Methicllin-resistant Staphylococcus Aureus (MRSA) colonization rate among ruminant animals slaughtered for human consumption and contact persons in Maiduguri, Nigeria’’ AJMR, 8(27),2643-2649, 2014
[10]	Moodley A, Guarddbassi L, ‘’Clonal spread of coagulase negative staphylococci among horses, personnel, and environmental sites of equnine facilities’’.Vet Microbiol.137,397-401, 2009
[11]	Piesseens V, Van Coillie, Verbist B, Supre K, Braem G, van Nuffel A, De Vuyst L, Heyndridex De Vlieghert S. ‘’ Distribution of coagulase-negative staphylococci species from milk and environment of dairy cow differs between herds.’’ J.Dairy Sci.94, 2933-2944, 2011
[12]	Supre K, Haesebronck F, Zadoks,RN Vaneechvutta M, Pieperss V, De Vliegher S.’’ Some CNS species are affecting udder health more than others’’.J.Dairy Sci.94, 2329-2340.
[13]	Taponen S, Bjorroth J Pyorala S’’Coagulase negative staphylococci isolated from bovine extra mammary site and intramammary infection in a single herd.J.Dairy Res.75, 422-429, 2008
[14]	Thorberg BM, Danielssom-Tham ML, Emmanuelson U, Walker KP’’Bovine subclinical mastitis caused by different type of coagulase negative staphylococci.J.Dairy Sci.’’92, 4962-4970, 2009.
[15]	Thorberg BM, Kuhn I, Aarestrup FM, Brandstrom B, Jonsson P, Danielsson-Tharm. ‘’Pheno and genotyping of Staphylococcus epidermidis isolated from bovine milk and human skin’’ Vet Microbiol, 115, 163-172, 2006
[16]	Zhang Y, Agidi S, LeJenune JT.’’Diversity of staphylococcal cassette chromosome in coagulase negative staphylococci of animal origin.’’ Appl.Microbiol,107, 1375-1383,2009.

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APA Style

Mai-Siyama Bukar Ibrahim, Okon Kenneth Okwong, Adamu Nuhu Bala, Mohammed Abdulrahman, Hamidu Ibrahim, et al. (2015). Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria. Animal and Veterinary Sciences, 3(5), 128-131. https://doi.org/10.11648/j.avs.20150305.11

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ACS Style

Mai-Siyama Bukar Ibrahim; Okon Kenneth Okwong; Adamu Nuhu Bala; Mohammed Abdulrahman; Hamidu Ibrahim, et al. Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria. Anim. Vet. Sci. 2015, 3(5), 128-131. doi: 10.11648/j.avs.20150305.11

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AMA Style

Mai-Siyama Bukar Ibrahim, Okon Kenneth Okwong, Adamu Nuhu Bala, Mohammed Abdulrahman, Hamidu Ibrahim, et al. Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria. Anim Vet Sci. 2015;3(5):128-131. doi: 10.11648/j.avs.20150305.11

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@article{10.11648/j.avs.20150305.11,
  author = {Mai-Siyama Bukar Ibrahim and Okon Kenneth Okwong and Adamu Nuhu Bala and Mohammed Abdulrahman and Hamidu Ibrahim and Hambali Idris Umar and Jajere Saleh Mohammed},
  title = {Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {5},
  pages = {128-131},
  doi = {10.11648/j.avs.20150305.11},
  url = {https://doi.org/10.11648/j.avs.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150305.11},
  abstract = {Coagulase negative staphylococci (CNS) are primary cause of mastitis in ruminant animal, and can either be transmitted by contagious or by environmental means. Therefore, we speculate whether there are similarity in the species distribution of CNS colonsing the anterior nare and milk of ruminant animals that can serve as source of infection. The study examined the species of CNS from the anterior nare of ruminant animals and contact persons. A total of 75 CNS isolates were analysed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), mecA gene detection by PCR assay and antibiotic susceptibility testing according to CSLI guidelines. Overall, four CNS spp were identified, S.sciuri predominate 71 (96%) and were recovered from all samples analysed, while one specie each (1.3%) of S.lentus, S.condementii and S.xylosus (1.3%) respectively. No mecA gene was detected in the CNS spp identified. The CNS spp demonstrated low to moderate resistance pattern (15-30%) to all antibiotics tested. In conclusion, the study provided a baseline epidemiological information on CNS spp colonizing the ruminants ruminant animal and contact persons. In addition, non-detection of mecA gene and high sensitivity pattern demonstrated by the CNS species.},
 year = {2015}
}

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TY  - JOUR
T1  - Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria
AU  - Mai-Siyama Bukar Ibrahim
AU  - Okon Kenneth Okwong
AU  - Adamu Nuhu Bala
AU  - Mohammed Abdulrahman
AU  - Hamidu Ibrahim
AU  - Hambali Idris Umar
AU  - Jajere Saleh Mohammed
Y1  - 2015/07/21
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150305.11
DO  - 10.11648/j.avs.20150305.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 128
EP  - 131
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150305.11
AB  - Coagulase negative staphylococci (CNS) are primary cause of mastitis in ruminant animal, and can either be transmitted by contagious or by environmental means. Therefore, we speculate whether there are similarity in the species distribution of CNS colonsing the anterior nare and milk of ruminant animals that can serve as source of infection. The study examined the species of CNS from the anterior nare of ruminant animals and contact persons. A total of 75 CNS isolates were analysed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), mecA gene detection by PCR assay and antibiotic susceptibility testing according to CSLI guidelines. Overall, four CNS spp were identified, S.sciuri predominate 71 (96%) and were recovered from all samples analysed, while one specie each (1.3%) of S.lentus, S.condementii and S.xylosus (1.3%) respectively. No mecA gene was detected in the CNS spp identified. The CNS spp demonstrated low to moderate resistance pattern (15-30%) to all antibiotics tested. In conclusion, the study provided a baseline epidemiological information on CNS spp colonizing the ruminants ruminant animal and contact persons. In addition, non-detection of mecA gene and high sensitivity pattern demonstrated by the CNS species.
VL  - 3
IS  - 5
ER  -

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Author Information

Mai-Siyama Bukar Ibrahim

Department of Veterinary Public Health, University of Maiduguri, Maiduguri, Nigeria
Okon Kenneth Okwong

Department of Medical Microbiology, Federal Medical Centre, Makurdi, Nigeria
Adamu Nuhu Bala

Department of Veterinary Public Health, University of Maiduguri, Maiduguri, Nigeria
Mohammed Abdulrahman

Department of Veterinary Public Health, University of Maiduguri, Maiduguri, Nigeria
Hamidu Ibrahim

Department of Immunology and Infectious diseases, University of Maiduguri Teaching Hospital, Maiduguri, Nigeria
Hambali Idris Umar

Department of Veterinary Public Health, University of Maiduguri, Maiduguri, Nigeria
Jajere Saleh Mohammed

Department of Veterinary Public Health, University of Maiduguri, Maiduguri, Nigeria

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APA Style

Mai-Siyama Bukar Ibrahim, Okon Kenneth Okwong, Adamu Nuhu Bala, Mohammed Abdulrahman, Hamidu Ibrahim, et al. (2015). Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria. Animal and Veterinary Sciences, 3(5), 128-131. https://doi.org/10.11648/j.avs.20150305.11

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ACS Style

Mai-Siyama Bukar Ibrahim; Okon Kenneth Okwong; Adamu Nuhu Bala; Mohammed Abdulrahman; Hamidu Ibrahim, et al. Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria. Anim. Vet. Sci. 2015, 3(5), 128-131. doi: 10.11648/j.avs.20150305.11

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AMA Style

Mai-Siyama Bukar Ibrahim, Okon Kenneth Okwong, Adamu Nuhu Bala, Mohammed Abdulrahman, Hamidu Ibrahim, et al. Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria. Anim Vet Sci. 2015;3(5):128-131. doi: 10.11648/j.avs.20150305.11

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@article{10.11648/j.avs.20150305.11,
  author = {Mai-Siyama Bukar Ibrahim and Okon Kenneth Okwong and Adamu Nuhu Bala and Mohammed Abdulrahman and Hamidu Ibrahim and Hambali Idris Umar and Jajere Saleh Mohammed},
  title = {Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {5},
  pages = {128-131},
  doi = {10.11648/j.avs.20150305.11},
  url = {https://doi.org/10.11648/j.avs.20150305.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150305.11},
  abstract = {Coagulase negative staphylococci (CNS) are primary cause of mastitis in ruminant animal, and can either be transmitted by contagious or by environmental means. Therefore, we speculate whether there are similarity in the species distribution of CNS colonsing the anterior nare and milk of ruminant animals that can serve as source of infection. The study examined the species of CNS from the anterior nare of ruminant animals and contact persons. A total of 75 CNS isolates were analysed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), mecA gene detection by PCR assay and antibiotic susceptibility testing according to CSLI guidelines. Overall, four CNS spp were identified, S.sciuri predominate 71 (96%) and were recovered from all samples analysed, while one specie each (1.3%) of S.lentus, S.condementii and S.xylosus (1.3%) respectively. No mecA gene was detected in the CNS spp identified. The CNS spp demonstrated low to moderate resistance pattern (15-30%) to all antibiotics tested. In conclusion, the study provided a baseline epidemiological information on CNS spp colonizing the ruminants ruminant animal and contact persons. In addition, non-detection of mecA gene and high sensitivity pattern demonstrated by the CNS species.},
 year = {2015}
}

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TY  - JOUR
T1  - Species of Coagulase- Negative Staphylococci Isolated from Anterior Nare and Milk of Ruminant Animals and Contacts Persons in Maiduguri, Nigeria
AU  - Mai-Siyama Bukar Ibrahim
AU  - Okon Kenneth Okwong
AU  - Adamu Nuhu Bala
AU  - Mohammed Abdulrahman
AU  - Hamidu Ibrahim
AU  - Hambali Idris Umar
AU  - Jajere Saleh Mohammed
Y1  - 2015/07/21
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150305.11
DO  - 10.11648/j.avs.20150305.11
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 128
EP  - 131
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150305.11
AB  - Coagulase negative staphylococci (CNS) are primary cause of mastitis in ruminant animal, and can either be transmitted by contagious or by environmental means. Therefore, we speculate whether there are similarity in the species distribution of CNS colonsing the anterior nare and milk of ruminant animals that can serve as source of infection. The study examined the species of CNS from the anterior nare of ruminant animals and contact persons. A total of 75 CNS isolates were analysed using matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), mecA gene detection by PCR assay and antibiotic susceptibility testing according to CSLI guidelines. Overall, four CNS spp were identified, S.sciuri predominate 71 (96%) and were recovered from all samples analysed, while one specie each (1.3%) of S.lentus, S.condementii and S.xylosus (1.3%) respectively. No mecA gene was detected in the CNS spp identified. The CNS spp demonstrated low to moderate resistance pattern (15-30%) to all antibiotics tested. In conclusion, the study provided a baseline epidemiological information on CNS spp colonizing the ruminants ruminant animal and contact persons. In addition, non-detection of mecA gene and high sensitivity pattern demonstrated by the CNS species.
VL  - 3
IS  - 5
ER  -

Copy | Download