Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet

Ercan Keskin; Deniz Uluisik; Mehmet Altin

doi:doi:10.11648/j.avs.20150304.13

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Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet

Ercan Keskin, Deniz Uluisik, Mehmet Altin

Published in Animal and Veterinary Sciences (Volume 3, Issue 4)

Received: 9 June 2015 Accepted: 26 June 2015 Published: 7 July 2015

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Abstract

This study was conducted to assess the effects of L-carnitine on antioxidant enzymes in rats fed cholesterol rich diet. A total of 32 healthy male Wistar Albino rats were allocated to four groups. Animals of the first group were fed standard rat pellets, animals of the second group were fed standard rat pellets that contained 7.5 % cholesterol powder, animals of the third group were fed standard rat pellets and water contained 75 mg/l L-carnitine while those of the forth group were fed standard rat pellets that contained 7.5 % cholesterol and water that contained 75 mg/l L-carnitine for 40 days. On the 40 th day of the study, blood samples were taken from all animals and thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels were determined. The results showed that feeding with high cholesterol diet resulted significantly increase in TBARS level and decreases in GSH, SOD, GPx levels when compared to control group (p<0.05). L-carnitine addition to the high cholesterol diet significantly decreased in TBARS level and increased in GSH, SOD levels compared to cholesterol group (p<0.05). In conclusion, our results showed L-carnitine may be useful an antioxidant in hypercholesterolemic condition.

Published in	Animal and Veterinary Sciences (Volume 3, Issue 4)
DOI	10.11648/j.avs.20150304.13
Page(s)	113-116
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

L-carnitine, Cholesterol, Antioxidants, Lipid Peroxidation, Rat

References

[1]	Halliwell, B. and Gutteridge, J. M. (1990). Free radicals in biology and medicine. Oxford, United Kingdom: Clarendon Press.
[2]	Block, G., Dietrich, M., Norkus, E. P., Morrow, J. D., Hudes, M., Caan, B. and Packer, L. (2002). Factors Associated with Oxidative Stress in Human Populations. Am. J. Epidemiol., 156: 274-285.
[3]	Evans, J. L., Goldfine, I. D., Maddux, B. A. and Grodsky, G. M. (2002). Oxidative Stress and Stress-Activated Signaling Pathways: A Unifying Hypothesis of Type 2 Diabetes. Endocrine Reviews, 23(5): 599-622.
[4]	Khan, N. I., Naz, L. and Yasmeen, G. (2006). Obesity: an independent risk factor for systemic oxidative stress. Pakistan J. Pharm. Sci., 19(1): 62-65.
[5]	Marseglia, L., Manti, S., D'Angelo, G., Nicotera, A., Parisi, E., Di Rosa, G., Gitto, E. and Arrigo, T. (2015). Oxidative stress in obesity: a critical component in human diseases. Int. J. Mol. Sci., 16(1): 378-400.
[6]	Keaney, J. F. and Vita, J. A. (1995). Atherosclerosis, Oxidative Stress, and Antioxidant Protection in Endothelium-Derived Relaxing Factor Action. Prog. Cardiovasc. Dis., 38(2): 129-154.
[7]	Polat, M. F., Taysi, S., Gül, M., Çıkman, O., Yılmaz, I., Bakan, E. and Erdogan, F. (2002). Oxidant/antioxidant status in blood of patients with malignant breast tumor and benign breast disease. Cell Biochemistry Function, 20(4): 327-331.
[8]	Taysi, S., Polat, F., Gül, M., Sarı, R. A. and Bakan, E. (2002). Lipid peroxidation, some extracellular antioxidants and antioxidant enzymes in serum of patients with rheumatoid arthritis. Rheumatology International, 21(5): 200-204.
[9]	Taysi, S., Uslu, C., Akçay, F. and Sütbeyaz, M. Y. (2002). Levels of malondialdehyde and nitric oxide in plasma of patients with advanced laryngeal cancer. Surgery Today, 33: 651-654.
[10]	Mansour, H. H. (2006). Protective role of carnitine ester against radiation-induced oxidative stress in rats. Pharmacological Research, 54(3): 165-171.
[11]	Demirdağ, K., Bahcecioğlu, I. H., Ozercan, I. H., Ozden, M., Yilmaz, S. and Kalkan, A. (2004). Role of L-carnitine in the prevention of acute liver damage induced by carbon tetrachloride in rats. Journal of Gastroenterology and Hepatology, 19(3): 333-338.
[12]	Sener, G., Paskaloğlu, K., Satiroglu, H., Alican, I., Kaçmaz, A. and Sakarcan, A. (2004). L-carnitine ameliorates oxidative damage due to chronic renal failure in rats. Journal of Cardiovascular Pharmacology, 43(5): 698-705.
[13]	El-khishin, I. A. and Amer, M. G. (2010). Possible Protective Role of L-carnitine on Diclofenac Induced Hepatotoxicity in Adult Male Albino Rats (Histological, Immunohistochemical and Biochemical Study). Egyptian Journal of Histology, 33(2): 341-352.
[14]	Izgut-Uysal, V. N., Ağaç, A. and Derin, N. (2001). Effect of carnitine on stress-induced lipid peroxidation in rat gastric mucosa. Journal of Gastroenterology, 36(4): 231-236.
[15]	Noeman, S. A., Hamooda, H. E. and Baalash, A. A. (2011). Biochemical Study of Oxidative Stress Markers in the Liver, Kidney and Heart of High Fat Diet Induced Obesity in Rats. Diabetology & Metabolic Syndrome, 3(17): 1-8.
[16]	Mishra, K. P. (2004). Cell membrane oxidative damage induced by gamma radiation and apoptotic sensitivity. Journal of Environmental Pathology, Toxicology and Oncology, 23(1): 61-66.
[17]	Blokhina, O., Virolainen, E. and Fagerstedt, K. V. (2003). Antioxidants, Oxidative Damage and Oxygen Deprivation Stress a Review. Annals Botany, 91: 179-194.
[18]	Sayed-Ahmed, M. M., Khattab, M. M., Gad, M. Z. and Mostafa, N. (2001). L-carnitine prevents the progression of atherosclerotic lesions in hypercholesterolaemic rabbits. Pharmacological Research, 44(3): 235-242.
[19]	Steinberg, D. (1995). Role of oxidized LDL and antioxidants in atherosclerosis. Advances Experimental Medicine and Biology, 369: 39-48.
[20]	Seccombe, D. W., James, L., Hahn, P. and Jones, E. (1987). L-carnitine treatment in the hyperlipidemic rabbit. Metabolism, 35: 1192-1196.
[21]	Spagnoli, L. G., Orlandi, A., Mauriello, A., DeAngelis, C. and Ramacci, M. T. (1995). Propionyl-l-carnitine prevents the progression of atherosclerotic lesions in aged hyperlipemic rabbits. Atherosclerosis, 114: 28-44.
[22]	Hiatt, W. R., Nawaz, D. and Brass, E. P. (1987). Carnitine metabolism during exercise in patients with peripheral vascular disease. J. Appl. Physiol., 62(6): 2383-2387.
[23]	Hiatt, W. R. and Brass, E. P. (1992). Carnitine metabolism in peripheral arterial disease. In: L-carnitine and its role in medicine: from function to therapy. Ferrari, R., Dimauro, S., Sherwood, G., Eds. London NW1 7DX: Academic Press Limited, 354-363.
[24]	Dayanandan, A., Kumar, P. and Panneerselvam, C. (2001). Protective role of L-carnitine on liver and heart lipid peroxidation in atherosclerotic rats. Journal of Nutritional Biochemistry, 12: 254-257.
[25]	Carter, A. L., Abney, P. O. and Lapp, F. D. (1995). Biosynthesis and metabolism of carnitine. Journal of Child Neurology, 10(2): 253-257.
[26]	Flanagan, J. L., Simmons, P. A., Vehige, J., Willcox, M. DP. and Garrett, Q. (2010). Role of carnitine in disease. Nutrition and Metabolism, 7: 1-14.
[27]	Seccombe, D. W., James, L., Peter, H. and Jones, E. (1987). L-carnitine treatment in hyperlipidemic rabbit. Metabolism, 36(12): 1192-1196.
[28]	Rajasekar, P., Ravichandran, M. K. and Anuradha, C. V. (2005). Intraperitoneal L-carnitine regulates lipid metabolism and reduces oxidative stress in fructose-induced hyperlipidemic rats. Diabetologia Croatica, 34(3): 87-95.
[29]	Reznick, A. K., Kagen, V. E., Ransey, R., Tsuchiya, M., Serbinova, E. A. and Packer, L. (1992). Antiradical effect in L-propionyl carnitine protection of the heart against ischemic reperfusion injury. The possible role of ion chelation. Archives Biochemistry Biophysics, 296(2): 394-401.
[30]	SushamaKumari, S., Jayadeep, A., Kumar, J. S. and Menon, V. P. (1989). Effect of carnitine on malondyaldehyde, taurine and glutathione levels in the heart of rats subjected to myocardial stress by isoproteinol. Indian Journal of Experimental Biology, 27(2): 134-137.

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APA Style

Ercan Keskin, Deniz Uluisik, Mehmet Altin. (2015). Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet. Animal and Veterinary Sciences, 3(4), 113-116. https://doi.org/10.11648/j.avs.20150304.13

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ACS Style

Ercan Keskin; Deniz Uluisik; Mehmet Altin. Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet. Anim. Vet. Sci. 2015, 3(4), 113-116. doi: 10.11648/j.avs.20150304.13

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AMA Style

Ercan Keskin, Deniz Uluisik, Mehmet Altin. Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet. Anim Vet Sci. 2015;3(4):113-116. doi: 10.11648/j.avs.20150304.13

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@article{10.11648/j.avs.20150304.13,
  author = {Ercan Keskin and Deniz Uluisik and Mehmet Altin},
  title = {Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {4},
  pages = {113-116},
  doi = {10.11648/j.avs.20150304.13},
  url = {https://doi.org/10.11648/j.avs.20150304.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150304.13},
  abstract = {This study was conducted to assess the effects of L-carnitine on antioxidant enzymes in rats fed cholesterol rich diet. A total of 32 healthy male Wistar Albino rats were allocated to four groups. Animals of the first group were fed standard rat pellets, animals of the second group were fed standard rat pellets that contained 7.5 % cholesterol powder, animals of the third group were fed standard rat pellets and water contained 75 mg/l L-carnitine while those of the forth group were fed standard rat pellets that contained 7.5 % cholesterol and water that contained 75 mg/l L-carnitine for 40 days. On the 40 th day of the study, blood samples were taken from all animals and thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels were determined. The results showed that feeding with high cholesterol diet resulted significantly increase in TBARS level and decreases in GSH, SOD, GPx levels when compared to control group (p<0.05). L-carnitine addition to the high cholesterol diet significantly decreased in TBARS level and increased in GSH, SOD levels compared to cholesterol group (p<0.05). In conclusion, our results showed L-carnitine may be useful an antioxidant in hypercholesterolemic condition.},
 year = {2015}
}

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TY  - JOUR
T1  - Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet
AU  - Ercan Keskin
AU  - Deniz Uluisik
AU  - Mehmet Altin
Y1  - 2015/07/07
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150304.13
DO  - 10.11648/j.avs.20150304.13
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 113
EP  - 116
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150304.13
AB  - This study was conducted to assess the effects of L-carnitine on antioxidant enzymes in rats fed cholesterol rich diet. A total of 32 healthy male Wistar Albino rats were allocated to four groups. Animals of the first group were fed standard rat pellets, animals of the second group were fed standard rat pellets that contained 7.5 % cholesterol powder, animals of the third group were fed standard rat pellets and water contained 75 mg/l L-carnitine while those of the forth group were fed standard rat pellets that contained 7.5 % cholesterol and water that contained 75 mg/l L-carnitine for 40 days. On the 40 th day of the study, blood samples were taken from all animals and thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels were determined. The results showed that feeding with high cholesterol diet resulted significantly increase in TBARS level and decreases in GSH, SOD, GPx levels when compared to control group (p<0.05). L-carnitine addition to the high cholesterol diet significantly decreased in TBARS level and increased in GSH, SOD levels compared to cholesterol group (p<0.05). In conclusion, our results showed L-carnitine may be useful an antioxidant in hypercholesterolemic condition.
VL  - 3
IS  - 4
ER  -

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Author Information

Ercan Keskin

Department of Physiology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey
Deniz Uluisik

Department of Physiology, Faculty of Veterinary Medicine, University of Selcuk, Konya, Turkey
Mehmet Altin

School of Physical Education and Sports, University of Selcuk, Konya, Turkey

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APA Style

Ercan Keskin, Deniz Uluisik, Mehmet Altin. (2015). Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet. Animal and Veterinary Sciences, 3(4), 113-116. https://doi.org/10.11648/j.avs.20150304.13

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ACS Style

Ercan Keskin; Deniz Uluisik; Mehmet Altin. Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet. Anim. Vet. Sci. 2015, 3(4), 113-116. doi: 10.11648/j.avs.20150304.13

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AMA Style

Ercan Keskin, Deniz Uluisik, Mehmet Altin. Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet. Anim Vet Sci. 2015;3(4):113-116. doi: 10.11648/j.avs.20150304.13

Copy | Download

@article{10.11648/j.avs.20150304.13,
  author = {Ercan Keskin and Deniz Uluisik and Mehmet Altin},
  title = {Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {4},
  pages = {113-116},
  doi = {10.11648/j.avs.20150304.13},
  url = {https://doi.org/10.11648/j.avs.20150304.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150304.13},
  abstract = {This study was conducted to assess the effects of L-carnitine on antioxidant enzymes in rats fed cholesterol rich diet. A total of 32 healthy male Wistar Albino rats were allocated to four groups. Animals of the first group were fed standard rat pellets, animals of the second group were fed standard rat pellets that contained 7.5 % cholesterol powder, animals of the third group were fed standard rat pellets and water contained 75 mg/l L-carnitine while those of the forth group were fed standard rat pellets that contained 7.5 % cholesterol and water that contained 75 mg/l L-carnitine for 40 days. On the 40 th day of the study, blood samples were taken from all animals and thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels were determined. The results showed that feeding with high cholesterol diet resulted significantly increase in TBARS level and decreases in GSH, SOD, GPx levels when compared to control group (p<0.05). L-carnitine addition to the high cholesterol diet significantly decreased in TBARS level and increased in GSH, SOD levels compared to cholesterol group (p<0.05). In conclusion, our results showed L-carnitine may be useful an antioxidant in hypercholesterolemic condition.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Antioxidant Effect of L-carnitine in Rats Fed Cholesterol Rich Diet
AU  - Ercan Keskin
AU  - Deniz Uluisik
AU  - Mehmet Altin
Y1  - 2015/07/07
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150304.13
DO  - 10.11648/j.avs.20150304.13
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 113
EP  - 116
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150304.13
AB  - This study was conducted to assess the effects of L-carnitine on antioxidant enzymes in rats fed cholesterol rich diet. A total of 32 healthy male Wistar Albino rats were allocated to four groups. Animals of the first group were fed standard rat pellets, animals of the second group were fed standard rat pellets that contained 7.5 % cholesterol powder, animals of the third group were fed standard rat pellets and water contained 75 mg/l L-carnitine while those of the forth group were fed standard rat pellets that contained 7.5 % cholesterol and water that contained 75 mg/l L-carnitine for 40 days. On the 40 th day of the study, blood samples were taken from all animals and thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) levels were determined. The results showed that feeding with high cholesterol diet resulted significantly increase in TBARS level and decreases in GSH, SOD, GPx levels when compared to control group (p<0.05). L-carnitine addition to the high cholesterol diet significantly decreased in TBARS level and increased in GSH, SOD levels compared to cholesterol group (p<0.05). In conclusion, our results showed L-carnitine may be useful an antioxidant in hypercholesterolemic condition.
VL  - 3
IS  - 4
ER  -

Copy | Download