Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.
| Published in | Animal and Veterinary Sciences (Volume 3, Issue 4) |
| DOI | 10.11648/j.avs.20150304.11 |
| Page(s) | 102-105 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2015. Published by Science Publishing Group |
Toxoplasma gondii, Toxoplasmosis, Native Cattle, PCR, Iran
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APA Style
Asghar Arshi, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Doosti. (2015). Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Animal and Veterinary Sciences, 3(4), 102-105. https://doi.org/10.11648/j.avs.20150304.11
ACS Style
Asghar Arshi; Esmaeil Mahmoudi; Mohammad-Saeid Jami; Abbas Doosti. Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Anim. Vet. Sci. 2015, 3(4), 102-105. doi: 10.11648/j.avs.20150304.11
AMA Style
Asghar Arshi, Esmaeil Mahmoudi, Mohammad-Saeid Jami, Abbas Doosti. Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran. Anim Vet Sci. 2015;3(4):102-105. doi: 10.11648/j.avs.20150304.11
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Download@article{10.11648/j.avs.20150304.11,
author = {Asghar Arshi and Esmaeil Mahmoudi and Mohammad-Saeid Jami and Abbas Doosti},
title = {Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran},
journal = {Animal and Veterinary Sciences},
volume = {3},
number = {4},
pages = {102-105},
doi = {10.11648/j.avs.20150304.11},
url = {https://doi.org/10.11648/j.avs.20150304.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150304.11},
abstract = {Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses.},
year = {2015}
}
TY - JOUR T1 - Detection of Toxoplasma gondii from Native Cattle in Southwest of Iran AU - Asghar Arshi AU - Esmaeil Mahmoudi AU - Mohammad-Saeid Jami AU - Abbas Doosti Y1 - 2015/06/12 PY - 2015 N1 - https://doi.org/10.11648/j.avs.20150304.11 DO - 10.11648/j.avs.20150304.11 T2 - Animal and Veterinary Sciences JF - Animal and Veterinary Sciences JO - Animal and Veterinary Sciences SP - 102 EP - 105 PB - Science Publishing Group SN - 2328-5850 UR - https://doi.org/10.11648/j.avs.20150304.11 AB - Infections by protozoan parasite Toxoplasma gondii are prevalent worldwide in animals and human. T. gondii is the causative agent of toxoplasmosis, one of the most prevalent parasitic infections to humans and domestic animals. If first be during pregnancy, T. gondii may be transferred vertically by tachyzoites that are passed to the embryo via the placenta. T. gondii may be transmitted horizontally in three phases of the life cycle, ingesting infectious oocysts from the environment or tissue cysts or tachyzoites which are contained in gastrointestinal of many different animals. Transmission may also occur via tachyzoites contained in blood products, texture transplants or non-pasteurized milk. Like rest of the world toxoplasmosis is prevalent in Iran. The present study aimed to determine T. gondii isolates from native cattle in Chaharmahal Va Bakhtiari province located in south west of Iran by molecular methods. In this study, 155 blood samples were collected from native cattle. Genomic DNA was extracted using DNA extraction Kit (Cinna Gen, Iran) according to the manufacturer protocol and PCR was performed using specific primers (ITS-F and ITS-R). Sixteen (6.95%) cattle were positive to T. gondii infection. The positive control samples showed the excepted amplification product specific for T. gondii (171 bp). Although the present results showed relatively low prevalence of T.gondii infection in Chaharmahal Va Bakhtiari native cattle, control and eradication programs seem to be still necessary to prevent the prevalence of this infection factor and economic losses. VL - 3 IS - 4 ER -
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