Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors

Ahmed Hassan; Mostafa Shahata; Elrefaie Refaie; Ragab Ibrahim; Jun Sasaki; Masanubu Goryo

doi:doi:10.11648/j.avs.20150302.13

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Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors

Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, Masanubu Goryo

Published in Animal and Veterinary Sciences (Volume 3, Issue 2)

Received: 18 October 2014 Accepted: 3 November 2014 Published: 28 February 2015

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Abstract

Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.

Published in	Animal and Veterinary Sciences (Volume 3, Issue 2)
DOI	10.11648/j.avs.20150302.13
Page(s)	46-50
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Infectious Bursal Disease Virus, Cell Lines, Histopathological Staining, Immunohistochemistry, Immunofluorescence

References

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APA Style

Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. (2015). Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Animal and Veterinary Sciences, 3(2), 46-50. https://doi.org/10.11648/j.avs.20150302.13

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ACS Style

Ahmed Hassan; Mostafa Shahata; Elrefaie Refaie; Ragab Ibrahim; Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim. Vet. Sci. 2015, 3(2), 46-50. doi: 10.11648/j.avs.20150302.13

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AMA Style

Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim Vet Sci. 2015;3(2):46-50. doi: 10.11648/j.avs.20150302.13

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@article{10.11648/j.avs.20150302.13,
  author = {Ahmed Hassan and Mostafa Shahata and Elrefaie Refaie and Ragab Ibrahim and Jun Sasaki and Masanubu Goryo},
  title = {Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {2},
  pages = {46-50},
  doi = {10.11648/j.avs.20150302.13},
  url = {https://doi.org/10.11648/j.avs.20150302.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150302.13},
  abstract = {Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.},
 year = {2015}
}

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TY  - JOUR
T1  - Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors
AU  - Ahmed Hassan
AU  - Mostafa Shahata
AU  - Elrefaie Refaie
AU  - Ragab Ibrahim
AU  - Jun Sasaki
AU  - Masanubu Goryo
Y1  - 2015/02/28
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150302.13
DO  - 10.11648/j.avs.20150302.13
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 46
EP  - 50
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150302.13
AB  - Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.
VL  - 3
IS  - 2
ER  -

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Author Information

Ahmed Hassan

Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan
Mostafa Shahata

Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt
Elrefaie Refaie

Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt
Ragab Ibrahim

Department of Poultry diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt
Jun Sasaki

Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan
Masanubu Goryo

Department of Veterinary Pathology, Faculty of Agriculture, Iwate University, 3-18-8, Ueda, Morioka, 020-8550, Japan

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APA Style

Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. (2015). Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Animal and Veterinary Sciences, 3(2), 46-50. https://doi.org/10.11648/j.avs.20150302.13

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ACS Style

Ahmed Hassan; Mostafa Shahata; Elrefaie Refaie; Ragab Ibrahim; Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim. Vet. Sci. 2015, 3(2), 46-50. doi: 10.11648/j.avs.20150302.13

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AMA Style

Ahmed Hassan, Mostafa Shahata, Elrefaie Refaie, Ragab Ibrahim, Jun Sasaki, et al. Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors. Anim Vet Sci. 2015;3(2):46-50. doi: 10.11648/j.avs.20150302.13

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@article{10.11648/j.avs.20150302.13,
  author = {Ahmed Hassan and Mostafa Shahata and Elrefaie Refaie and Ragab Ibrahim and Jun Sasaki and Masanubu Goryo},
  title = {Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors},
  journal = {Animal and Veterinary Sciences},
  volume = {3},
  number = {2},
  pages = {46-50},
  doi = {10.11648/j.avs.20150302.13},
  url = {https://doi.org/10.11648/j.avs.20150302.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.avs.20150302.13},
  abstract = {Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.},
 year = {2015}
}

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TY  - JOUR
T1  - Growth of Different Infectious Bursal Disease Virus Strains in Cell Lines from Origin of Lymphoid Leukosis Tumors
AU  - Ahmed Hassan
AU  - Mostafa Shahata
AU  - Elrefaie Refaie
AU  - Ragab Ibrahim
AU  - Jun Sasaki
AU  - Masanubu Goryo
Y1  - 2015/02/28
PY  - 2015
N1  - https://doi.org/10.11648/j.avs.20150302.13
DO  - 10.11648/j.avs.20150302.13
T2  - Animal and Veterinary Sciences
JF  - Animal and Veterinary Sciences
JO  - Animal and Veterinary Sciences
SP  - 46
EP  - 50
PB  - Science Publishing Group
SN  - 2328-5850
UR  - https://doi.org/10.11648/j.avs.20150302.13
AB  - Growth and propagation of infectious bursal disease virus (IBDV) in chicken embryo is time consuming and costly, and an appropriate serological test is required to detect and identify IBDV strains, therefore, a suitable cell lines in which different IBDV strains can grow well has been needed. The aim of the present work was to study the growth of different IBDV strains in cell lines from lymphoid leukosis tumors using histopathological staining, indirect immunofluorescence, immunohistochemistry and transmission electron microscopic examination. In conclusion, cell lines from origin of lymphoid leukosis tumors; LSCC-BK3 and LSCC-CU10 are suitable for growth and propagation of different IBDV strains. IBDV strains resulted into histopathological lesions of variable severity differ according to the patho-type of IBDV and time after virus inoculation. Indirect immunofluorescent test could be used for detection and differentiation of IBDV strains inoculated into cell lines from origin of lymphoid leukosis tumors. Trials of immunohistochemistry technique for detection of different IBDV strains in cell lines, were unsuccessful. Using transmission electron microscopy, IBDV particles could be detected in all infected cell lines.
VL  - 3
IS  - 2
ER  -

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