This paper introduces a method for simultaneous detection of 2019 novel coronavirus (2019-nCoV) and influenza virus A and influenza virus B (FluA/B) by dual fluorescent RT-PCR, providing some references for the current clinical first-line practice against the epidemic. More than 12000 samples of nasopharyngeal swabs, sputum and anal swabs were collected. All samples were inactivated at 60°C for 40 min before testing, so as to protect medical examine. Nucleic acid detection kits from two manufacturers of 2019-nCoV and FluA/B were selected for the detection. Carboxyfluorescein (FAM) and green fluorescent protein (VIC) labeled probes were used to achieve simultaneous detection of the four gene targets using a double fluorescent RT-PCR reaction system. According to the results of nucleic acid detection, there is no cross infection between 2019-nCoV and FluA/B. The CT value of novel coronavirus nucleic acid in anal swab greater than CT value of sputum greater than CT value of nasopharyngeal swab in the same patient. This paper introduces a method for rapid and simultaneous detection of 2019-nCoV and FluA/B by dual fluorescent RT-PCR. It was improved the detection efficiency and reduce the cost, which could be used for rapid and emergent detection of 2019-nCoV and FluA/B. Our experiment is fast and accurate, could make reference for nucleic acid detection medical diagnosis for clinical medical staff.
| Published in | American Journal of Laboratory Medicine (Volume 5, Issue 1) |
| DOI | 10.11648/j.ajlm.20200501.16 |
| Page(s) | 42-46 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2020. Published by Science Publishing Group |
2019-nCoV, A/B Influenza Virus, Nucleic Acid Detection, Double Fluorescent RT-PCR
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APA Style
Shasha Zhang, Xiaoli Sun, Wei Guo, Jianfeng Xu. (2020). Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR. American Journal of Laboratory Medicine, 5(1), 42-46. https://doi.org/10.11648/j.ajlm.20200501.16
ACS Style
Shasha Zhang; Xiaoli Sun; Wei Guo; Jianfeng Xu. Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR. Am. J. Lab. Med. 2020, 5(1), 42-46. doi: 10.11648/j.ajlm.20200501.16
AMA Style
Shasha Zhang, Xiaoli Sun, Wei Guo, Jianfeng Xu. Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR. Am J Lab Med. 2020;5(1):42-46. doi: 10.11648/j.ajlm.20200501.16
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Download@article{10.11648/j.ajlm.20200501.16,
author = {Shasha Zhang and Xiaoli Sun and Wei Guo and Jianfeng Xu},
title = {Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR},
journal = {American Journal of Laboratory Medicine},
volume = {5},
number = {1},
pages = {42-46},
doi = {10.11648/j.ajlm.20200501.16},
url = {https://doi.org/10.11648/j.ajlm.20200501.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20200501.16},
abstract = {This paper introduces a method for simultaneous detection of 2019 novel coronavirus (2019-nCoV) and influenza virus A and influenza virus B (FluA/B) by dual fluorescent RT-PCR, providing some references for the current clinical first-line practice against the epidemic. More than 12000 samples of nasopharyngeal swabs, sputum and anal swabs were collected. All samples were inactivated at 60°C for 40 min before testing, so as to protect medical examine. Nucleic acid detection kits from two manufacturers of 2019-nCoV and FluA/B were selected for the detection. Carboxyfluorescein (FAM) and green fluorescent protein (VIC) labeled probes were used to achieve simultaneous detection of the four gene targets using a double fluorescent RT-PCR reaction system. According to the results of nucleic acid detection, there is no cross infection between 2019-nCoV and FluA/B. The CT value of novel coronavirus nucleic acid in anal swab greater than CT value of sputum greater than CT value of nasopharyngeal swab in the same patient. This paper introduces a method for rapid and simultaneous detection of 2019-nCoV and FluA/B by dual fluorescent RT-PCR. It was improved the detection efficiency and reduce the cost, which could be used for rapid and emergent detection of 2019-nCoV and FluA/B. Our experiment is fast and accurate, could make reference for nucleic acid detection medical diagnosis for clinical medical staff.},
year = {2020}
}
TY - JOUR T1 - Simultaneous Detection of 2019 Novel Coronavirus and Influenza Virus by Double Fluorescent RT-PCR AU - Shasha Zhang AU - Xiaoli Sun AU - Wei Guo AU - Jianfeng Xu Y1 - 2020/04/13 PY - 2020 N1 - https://doi.org/10.11648/j.ajlm.20200501.16 DO - 10.11648/j.ajlm.20200501.16 T2 - American Journal of Laboratory Medicine JF - American Journal of Laboratory Medicine JO - American Journal of Laboratory Medicine SP - 42 EP - 46 PB - Science Publishing Group SN - 2575-386X UR - https://doi.org/10.11648/j.ajlm.20200501.16 AB - This paper introduces a method for simultaneous detection of 2019 novel coronavirus (2019-nCoV) and influenza virus A and influenza virus B (FluA/B) by dual fluorescent RT-PCR, providing some references for the current clinical first-line practice against the epidemic. More than 12000 samples of nasopharyngeal swabs, sputum and anal swabs were collected. All samples were inactivated at 60°C for 40 min before testing, so as to protect medical examine. Nucleic acid detection kits from two manufacturers of 2019-nCoV and FluA/B were selected for the detection. Carboxyfluorescein (FAM) and green fluorescent protein (VIC) labeled probes were used to achieve simultaneous detection of the four gene targets using a double fluorescent RT-PCR reaction system. According to the results of nucleic acid detection, there is no cross infection between 2019-nCoV and FluA/B. The CT value of novel coronavirus nucleic acid in anal swab greater than CT value of sputum greater than CT value of nasopharyngeal swab in the same patient. This paper introduces a method for rapid and simultaneous detection of 2019-nCoV and FluA/B by dual fluorescent RT-PCR. It was improved the detection efficiency and reduce the cost, which could be used for rapid and emergent detection of 2019-nCoV and FluA/B. Our experiment is fast and accurate, could make reference for nucleic acid detection medical diagnosis for clinical medical staff. VL - 5 IS - 1 ER -
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