The main goal of this study was to assess the cytological evaluation of tracheal aspirate, in order to predict the early diagnosis of bronchopulmonary dysplasia (BPD) in high-risk babies during mechanical ventilation. Method: Samples from 43 infants (aged < 34 weeks and weighed ≤ 1500 g) were examined cytologically; who were admitted to Pediatric Intensive Care Units and diagnosed as respiratory distress syndrome by clinical and radiological criteria. Results: Up to 150 tracheal aspirates from 43 infants were examined to demonstrate cytological changes in bronchial epithelial cells that progressed to dysplasia. It was cytologically designated Class III. According to cytological criteria, all intubated infants had reactive hyperplastic and metaplastic epithelial changes. There were dysplastic changes in 23 out of 33 who developed BPD and 7 out of 40 who had a normal outcome. The variation between the numbers of neonates that had dysplasia in the BPD and the non-BPD group was significant (P < 0.002 by chi-square test). Conclusion: In order to monitor the development of lung injury and repair, tracheal aspirate cytology is simple, non-invasive and fast. Cytological monitoring of respiratory distress infants may be helpful in the early diagnosis and prognosis of the developing BPD. Evaluating new BPD preventive treatment schemes may also be valuable.
| Published in | American Journal of Laboratory Medicine (Volume 4, Issue 3) |
| DOI | 10.11648/j.ajlm.20190403.13 |
| Page(s) | 65-69 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2019. Published by Science Publishing Group |
Bronchopulmonary Dysplasia (BPD), Cytology, Microscopic, Neonates, Predict, Tracheal Aspirate
| [1] | LaGrandeur RG, Singhal M, Bany-Mohammed F, et al. Pilot feasibility study to detect mesenchymal stem cell biomarkers of bronchopulmonary dysplasia in the tracheal aspirate fluid of preterm infants. Journal of Neonatal-Perinatal Medicine 2018; 11 (1) DOI 10.3233/NPM-181722. |
| [2] | Lal CVand Ambalavanan N. Biomarkers Early Diagnosis, and Clinical Predictors of Bronchopulmonary DysplasiaClin Perinatol. 2015; 42 (4): 739˗54. doi: 10.1016/j.clp. 2015.08.004. Epub 2015 Oct 1. |
| [3] | Nickerson BG. Bronchopulmonary dysplasia. Chronic pulmonary disease following neonatal respiratory failure. Chest 1985 Apr; 87 (4): 528-35. |
| [4] | Jobe AH, Bancalari E. Bronchopulmonary dysplasia. Am J Respir Crit Care Med 2001; 163 (7): 1723-9. |
| [5] | Ehrenkranz RA, Walsh MC, Vohr BR, et al. Validation of the National Institutes of Health consensus definition of bronchopulmonary dysplasia. Pediatrics 2005; 116 (6): 1353-60. |
| [6] | Bancalari E, Claure N, Sosenko IR. Bronchopulmonary dysplasia: Changes in pathogenesis, epidemiology and definition. Semin Neonatol 2003; 8 (1): 63-71. |
| [7] | Le Cras TD, Kim DH, Markham NE, Abman AS. Early abnormalities of pulmonary vascular development in the Fawn-Hooded rat raised at Denver’s altitude. Am J Physiol Lung Cell Mol Physiol 2000; 279 (2): L283-91. |
| [8] | Anderson PJ, Doyle LW. Neurodevelopmental outcome of bronchopulmonary dysplasia. Semin Perinatol 2006; 30 (4): 227-32. |
| [9] | Majnemer A, Riley P, Shevell M, Birnbaum R, Greenstone H, Coates AL. Severe bronchopulmonary dysplasia increases risk for later neurological and motor sequelae in preterm survivors. Dev Med Child Neurol 2000; 42 (1): 53-60. |
| [10] | Kinsella JP, Greenough A, Abman SH. Bronchopulmonary dysplasia. Lancet 2006; 367 (9520): 1421-31. |
| [11] | Baveja R, Christou H. Pharmacological strategies in the prevention and management of bronchopulmonary dysplasia. Semin Perinatol 2006; 30 (4): 209-18. |
| [12] | Todd DA, Earl M, Lloyd J, Greenberg M, John E. Cytological changes in endotracheal aspirates associated with chronic lung disease. Early Human Development 1998 51: 13–22. |
| [13] | Merritt TA, Stuard D, Puccia J, et al. Newborn tracheal aspirate cytology: classification during respiratory distress syndrome and bronchopulmonary dysplasia. J Pediatr 198 1; 98: 949-56. |
| [14] | Ambalavanan N and Novak ZE. Peptide Growth Factors in Tracheal Aspirates of Mechanically Ventilated Preterm Neonates. PEDIATRIC RESEARCH 2003; 53 (2): 240-244. |
| [15] | Merritt TA, Puccia JM, Stuard D. Cytologic evaluation of pulmonary effluent in neonates with respiratory distress syndrome and bronchopulmonary dysplasia. Acta Cytol 198 1; 25: 63 1-9. |
| [16] | D 'Ablang G. Bernard B, Zaharov 1, et al. Neonatal pulmonary cytology and bronchopulmonary dysplasia. Acta Cytol 1973; 19: 21-7. |
| [17] | Jacobson W, Morley CJ, South M. Microscopic observations on tracheal aspirates from ventilated neonates II. The onset of bronchopulmonary dysplasia and other changes. Eur J Pediatr 1992; 151: 204-7. |
| [18] | Doshi N, Kanbour A, Fujikura T, Klionsky B. Tracheal aspiration cytology with respiratory distress: histopathologic correlation. Acta Cytol 1982; 26: 15–21. |
| [19] | Noack G, Mortensson W, Robertson B, Nilsson R. Correlations between radiological and cytological findings in early development of bronchopulmonary dysplasia. Eur J Pediatr 1993; 152: 1024–9. |
| [20] | Rothberg AD, Miot A, Leiman G. Tracheal Aspirate Cytology and Bronchopulmonary Dysplasia. Diagnostic Cytopathology 1986; 2 (3): 212-216. |
| [21] | Zhekova N, Perenovska P, Kalaĭdzhieva M, Pramatarova T. [Diagnostic importance of the cytologic examination of tracheal lavage fluid in high-risk newborn infants]. Akush Ginekol (Sofiia). 2007; 46 Suppl 1: 9-13. |
APA Style
Sahar Ali Mohamed, Hossam Eldin Mohammed. (2019). Tracheal Aspirate Cytology of 43 High-Risk Neonates to Predict Bronchopulmonary Dysplasia Early. American Journal of Laboratory Medicine, 4(3), 65-69. https://doi.org/10.11648/j.ajlm.20190403.13
ACS Style
Sahar Ali Mohamed; Hossam Eldin Mohammed. Tracheal Aspirate Cytology of 43 High-Risk Neonates to Predict Bronchopulmonary Dysplasia Early. Am. J. Lab. Med. 2019, 4(3), 65-69. doi: 10.11648/j.ajlm.20190403.13
AMA Style
Sahar Ali Mohamed, Hossam Eldin Mohammed. Tracheal Aspirate Cytology of 43 High-Risk Neonates to Predict Bronchopulmonary Dysplasia Early. Am J Lab Med. 2019;4(3):65-69. doi: 10.11648/j.ajlm.20190403.13
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Download@article{10.11648/j.ajlm.20190403.13,
author = {Sahar Ali Mohamed and Hossam Eldin Mohammed},
title = {Tracheal Aspirate Cytology of 43 High-Risk Neonates to Predict Bronchopulmonary Dysplasia Early},
journal = {American Journal of Laboratory Medicine},
volume = {4},
number = {3},
pages = {65-69},
doi = {10.11648/j.ajlm.20190403.13},
url = {https://doi.org/10.11648/j.ajlm.20190403.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20190403.13},
abstract = {The main goal of this study was to assess the cytological evaluation of tracheal aspirate, in order to predict the early diagnosis of bronchopulmonary dysplasia (BPD) in high-risk babies during mechanical ventilation. Method: Samples from 43 infants (aged < 34 weeks and weighed ≤ 1500 g) were examined cytologically; who were admitted to Pediatric Intensive Care Units and diagnosed as respiratory distress syndrome by clinical and radiological criteria. Results: Up to 150 tracheal aspirates from 43 infants were examined to demonstrate cytological changes in bronchial epithelial cells that progressed to dysplasia. It was cytologically designated Class III. According to cytological criteria, all intubated infants had reactive hyperplastic and metaplastic epithelial changes. There were dysplastic changes in 23 out of 33 who developed BPD and 7 out of 40 who had a normal outcome. The variation between the numbers of neonates that had dysplasia in the BPD and the non-BPD group was significant (P < 0.002 by chi-square test). Conclusion: In order to monitor the development of lung injury and repair, tracheal aspirate cytology is simple, non-invasive and fast. Cytological monitoring of respiratory distress infants may be helpful in the early diagnosis and prognosis of the developing BPD. Evaluating new BPD preventive treatment schemes may also be valuable.},
year = {2019}
}
TY - JOUR T1 - Tracheal Aspirate Cytology of 43 High-Risk Neonates to Predict Bronchopulmonary Dysplasia Early AU - Sahar Ali Mohamed AU - Hossam Eldin Mohammed Y1 - 2019/07/12 PY - 2019 N1 - https://doi.org/10.11648/j.ajlm.20190403.13 DO - 10.11648/j.ajlm.20190403.13 T2 - American Journal of Laboratory Medicine JF - American Journal of Laboratory Medicine JO - American Journal of Laboratory Medicine SP - 65 EP - 69 PB - Science Publishing Group SN - 2575-386X UR - https://doi.org/10.11648/j.ajlm.20190403.13 AB - The main goal of this study was to assess the cytological evaluation of tracheal aspirate, in order to predict the early diagnosis of bronchopulmonary dysplasia (BPD) in high-risk babies during mechanical ventilation. Method: Samples from 43 infants (aged < 34 weeks and weighed ≤ 1500 g) were examined cytologically; who were admitted to Pediatric Intensive Care Units and diagnosed as respiratory distress syndrome by clinical and radiological criteria. Results: Up to 150 tracheal aspirates from 43 infants were examined to demonstrate cytological changes in bronchial epithelial cells that progressed to dysplasia. It was cytologically designated Class III. According to cytological criteria, all intubated infants had reactive hyperplastic and metaplastic epithelial changes. There were dysplastic changes in 23 out of 33 who developed BPD and 7 out of 40 who had a normal outcome. The variation between the numbers of neonates that had dysplasia in the BPD and the non-BPD group was significant (P < 0.002 by chi-square test). Conclusion: In order to monitor the development of lung injury and repair, tracheal aspirate cytology is simple, non-invasive and fast. Cytological monitoring of respiratory distress infants may be helpful in the early diagnosis and prognosis of the developing BPD. Evaluating new BPD preventive treatment schemes may also be valuable. VL - 4 IS - 3 ER -
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