Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt

Mohamed Ahmed Abdeldayem; Kadry Abdelkader El-bakry; Abdullah El-Bialy

doi:doi:10.11648/j.ajlm.20190403.11

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Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt

Mohamed Ahmed Abdeldayem, Kadry Abdelkader El-bakry, Abdullah El-Bialy

Published in American Journal of Laboratory Medicine (Volume 4, Issue 3)

Received: 30 April 2019 Accepted: 2 June 2019 Published: 24 June 2019

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Abstract

Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.

Published in	American Journal of Laboratory Medicine (Volume 4, Issue 3)
DOI	10.11648/j.ajlm.20190403.11
Page(s)	53-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Hepatitis C Virus, Occult Hepatitis C Virus, PBMCs, Hepatitis C Virus Core Ag

References

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APA Style

Mohamed Ahmed Abdeldayem, Kadry Abdelkader El-bakry, Abdullah El-Bialy. (2019). Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt. American Journal of Laboratory Medicine, 4(3), 53-59. https://doi.org/10.11648/j.ajlm.20190403.11

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ACS Style

Mohamed Ahmed Abdeldayem; Kadry Abdelkader El-bakry; Abdullah El-Bialy. Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt. Am. J. Lab. Med. 2019, 4(3), 53-59. doi: 10.11648/j.ajlm.20190403.11

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AMA Style

Mohamed Ahmed Abdeldayem, Kadry Abdelkader El-bakry, Abdullah El-Bialy. Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt. Am J Lab Med. 2019;4(3):53-59. doi: 10.11648/j.ajlm.20190403.11

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@article{10.11648/j.ajlm.20190403.11,
  author = {Mohamed Ahmed Abdeldayem and Kadry Abdelkader El-bakry and Abdullah El-Bialy},
  title = {Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt},
  journal = {American Journal of Laboratory Medicine},
  volume = {4},
  number = {3},
  pages = {53-59},
  doi = {10.11648/j.ajlm.20190403.11},
  url = {https://doi.org/10.11648/j.ajlm.20190403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20190403.11},
  abstract = {Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.},
 year = {2019}
}

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TY  - JOUR
T1  - Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt
AU  - Mohamed Ahmed Abdeldayem
AU  - Kadry Abdelkader El-bakry
AU  - Abdullah El-Bialy
Y1  - 2019/06/24
PY  - 2019
N1  - https://doi.org/10.11648/j.ajlm.20190403.11
DO  - 10.11648/j.ajlm.20190403.11
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 53
EP  - 59
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20190403.11
AB  - Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.
VL  - 4
IS  - 3
ER  -

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Author Information

Mohamed Ahmed Abdeldayem

Department of Zoology, Faculty of Science, Damietta University, Damietta, Egypt
Kadry Abdelkader El-bakry

Department of Physiology, Faculty of Science Damietta University, Damietta, Egypt
Abdullah El-Bialy

Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt

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APA Style

Mohamed Ahmed Abdeldayem, Kadry Abdelkader El-bakry, Abdullah El-Bialy. (2019). Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt. American Journal of Laboratory Medicine, 4(3), 53-59. https://doi.org/10.11648/j.ajlm.20190403.11

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ACS Style

Mohamed Ahmed Abdeldayem; Kadry Abdelkader El-bakry; Abdullah El-Bialy. Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt. Am. J. Lab. Med. 2019, 4(3), 53-59. doi: 10.11648/j.ajlm.20190403.11

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AMA Style

Mohamed Ahmed Abdeldayem, Kadry Abdelkader El-bakry, Abdullah El-Bialy. Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt. Am J Lab Med. 2019;4(3):53-59. doi: 10.11648/j.ajlm.20190403.11

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@article{10.11648/j.ajlm.20190403.11,
  author = {Mohamed Ahmed Abdeldayem and Kadry Abdelkader El-bakry and Abdullah El-Bialy},
  title = {Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt},
  journal = {American Journal of Laboratory Medicine},
  volume = {4},
  number = {3},
  pages = {53-59},
  doi = {10.11648/j.ajlm.20190403.11},
  url = {https://doi.org/10.11648/j.ajlm.20190403.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20190403.11},
  abstract = {Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.},
 year = {2019}
}

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TY  - JOUR
T1  - Occult Hepatitis C Virus in Blood Donors in Damietta - Egypt
AU  - Mohamed Ahmed Abdeldayem
AU  - Kadry Abdelkader El-bakry
AU  - Abdullah El-Bialy
Y1  - 2019/06/24
PY  - 2019
N1  - https://doi.org/10.11648/j.ajlm.20190403.11
DO  - 10.11648/j.ajlm.20190403.11
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 53
EP  - 59
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20190403.11
AB  - Egypt maintains the highest prevalence of hepatitis C virus infection, affecting an estimated 15%–20% of the population, new cases reported, so we search about uncommon source of hepatitis C virus infection. Occult hepatitis C Virus infection can be detected and founded in the overall public obviously haven’t any diseases, Detection of viral replication in PBMCs may increase spread and transmission of hepatitis C virus during blood transfusion, hemodialysis, and made liver disease development in occult Hepatitis C Virus infected individual. The present study aimed to detect the occult hepatitis C virus infection in healthy blood donors from Damietta-Egypt, And detection of Hepatitis C Virus core antigen. One hundred and fifty blood donors from El-Azhar University Hospital blood bank in New Damietta City were used into the present study. Detection of Hepatitis C Virus RNA in Peripheral blood mononuclear (PBMCs) and plasma by reverse transcription nested polymerase chain reaction (RT-PCR), and also detection of hepatitis C virus core antigen from plasma by ELISA. We founded that: Detection of hepatitis C virus RNA by nested PCR from PBMC among studied donors are eleven (7.9%) on the other hand Hepatitis C Virus RNA in plasma are two (1.4%) so occult C virus are 9/140 (6.4%). By comparison between results of Hepatitis C Virus core Ag and PCR evidence sensitivity 27%, specificity 98.4% in PBMC and sensitivity 100%, specificity 97.8% in Plasma.
VL  - 4
IS  - 3
ER  -

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