This study evaluated the influence of long-term simulated microgravity on oxidative stress and total antioxidant capacity in the plasma and lung tissues of rhesus macaque (-10℃ head-down tilting). Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n=5), groups 2 (head-down tilting for 6 weeks, n=5) and groups 3 (head-down tilting for 6 weeks and recover from 4 weeks, n=5). Oxidative stress was evaluated by critical SOD, GSH, H2O2 in plasma and SOD, GSH in lung tissues. HE staining was used to observe the histopathological structure changes of pulmonary tissues. CAT, SOD1, SOD2, SOD3, GPX1, GPX4, GPX7, PRDX1, HMOX1, ALOX5 and DUOX1 mRNA were measured by real-time PCR. GSH concentration was significantly decreased, whereas H2O2 level was significantly increased in group 2 compared with group 1 and group 3. Compared to group 1, histopathological examination revealed alveolar septal thickening, and alveolar and interstitial lymphocytic infiltration in group 2 and group 3 and the pathological changes in group 3 were smaller than those in group 2. Group 2 and group 3 showed significant up-regulation of SOD3 gene compared with group 1 by real-time PCR. In a long-term simulated microgravity environment, systemic antioxidant level of GSH was reduced but an oxidative stress marker of H2O2 was increased. Meanwhile, long-term simulated microgravity caused lung injury and induced the mRNA of SOD3 expression in lung tissues. But oxidant stress is not a major factor involved in the development of lung damage under simulated microgravity. Further study still clarifies the mechanism about the lung injury under microgravity.
| Published in | American Journal of Laboratory Medicine (Volume 2, Issue 1) |
| DOI | 10.11648/j.ajlm.20170201.11 |
| Page(s) | 1-6 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2017. Published by Science Publishing Group |
Simulated Microgravity, Oxidative Stress, GSH, H2O2
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APA Style
Yang Chen, Ping Wang, Chongyu Xu, Yiling Cai, Huasong Ma. (2017). Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque. American Journal of Laboratory Medicine, 2(1), 1-6. https://doi.org/10.11648/j.ajlm.20170201.11
ACS Style
Yang Chen; Ping Wang; Chongyu Xu; Yiling Cai; Huasong Ma. Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque. Am. J. Lab. Med. 2017, 2(1), 1-6. doi: 10.11648/j.ajlm.20170201.11
AMA Style
Yang Chen, Ping Wang, Chongyu Xu, Yiling Cai, Huasong Ma. Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque. Am J Lab Med. 2017;2(1):1-6. doi: 10.11648/j.ajlm.20170201.11
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Download@article{10.11648/j.ajlm.20170201.11,
author = {Yang Chen and Ping Wang and Chongyu Xu and Yiling Cai and Huasong Ma},
title = {Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque},
journal = {American Journal of Laboratory Medicine},
volume = {2},
number = {1},
pages = {1-6},
doi = {10.11648/j.ajlm.20170201.11},
url = {https://doi.org/10.11648/j.ajlm.20170201.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20170201.11},
abstract = {This study evaluated the influence of long-term simulated microgravity on oxidative stress and total antioxidant capacity in the plasma and lung tissues of rhesus macaque (-10℃ head-down tilting). Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n=5), groups 2 (head-down tilting for 6 weeks, n=5) and groups 3 (head-down tilting for 6 weeks and recover from 4 weeks, n=5). Oxidative stress was evaluated by critical SOD, GSH, H2O2 in plasma and SOD, GSH in lung tissues. HE staining was used to observe the histopathological structure changes of pulmonary tissues. CAT, SOD1, SOD2, SOD3, GPX1, GPX4, GPX7, PRDX1, HMOX1, ALOX5 and DUOX1 mRNA were measured by real-time PCR. GSH concentration was significantly decreased, whereas H2O2 level was significantly increased in group 2 compared with group 1 and group 3. Compared to group 1, histopathological examination revealed alveolar septal thickening, and alveolar and interstitial lymphocytic infiltration in group 2 and group 3 and the pathological changes in group 3 were smaller than those in group 2. Group 2 and group 3 showed significant up-regulation of SOD3 gene compared with group 1 by real-time PCR. In a long-term simulated microgravity environment, systemic antioxidant level of GSH was reduced but an oxidative stress marker of H2O2 was increased. Meanwhile, long-term simulated microgravity caused lung injury and induced the mRNA of SOD3 expression in lung tissues. But oxidant stress is not a major factor involved in the development of lung damage under simulated microgravity. Further study still clarifies the mechanism about the lung injury under microgravity.},
year = {2017}
}
TY - JOUR T1 - Effects of Long-Term Simulated Microgravity on Oxidant and Antioxidant Values in the Plasma and Lung Tissues of Rhesus Macaque AU - Yang Chen AU - Ping Wang AU - Chongyu Xu AU - Yiling Cai AU - Huasong Ma Y1 - 2017/01/18 PY - 2017 N1 - https://doi.org/10.11648/j.ajlm.20170201.11 DO - 10.11648/j.ajlm.20170201.11 T2 - American Journal of Laboratory Medicine JF - American Journal of Laboratory Medicine JO - American Journal of Laboratory Medicine SP - 1 EP - 6 PB - Science Publishing Group SN - 2575-386X UR - https://doi.org/10.11648/j.ajlm.20170201.11 AB - This study evaluated the influence of long-term simulated microgravity on oxidative stress and total antioxidant capacity in the plasma and lung tissues of rhesus macaque (-10℃ head-down tilting). Fifteen healthy male rhesus macaques were randomly divided into groups 1 (control, n=5), groups 2 (head-down tilting for 6 weeks, n=5) and groups 3 (head-down tilting for 6 weeks and recover from 4 weeks, n=5). Oxidative stress was evaluated by critical SOD, GSH, H2O2 in plasma and SOD, GSH in lung tissues. HE staining was used to observe the histopathological structure changes of pulmonary tissues. CAT, SOD1, SOD2, SOD3, GPX1, GPX4, GPX7, PRDX1, HMOX1, ALOX5 and DUOX1 mRNA were measured by real-time PCR. GSH concentration was significantly decreased, whereas H2O2 level was significantly increased in group 2 compared with group 1 and group 3. Compared to group 1, histopathological examination revealed alveolar septal thickening, and alveolar and interstitial lymphocytic infiltration in group 2 and group 3 and the pathological changes in group 3 were smaller than those in group 2. Group 2 and group 3 showed significant up-regulation of SOD3 gene compared with group 1 by real-time PCR. In a long-term simulated microgravity environment, systemic antioxidant level of GSH was reduced but an oxidative stress marker of H2O2 was increased. Meanwhile, long-term simulated microgravity caused lung injury and induced the mRNA of SOD3 expression in lung tissues. But oxidant stress is not a major factor involved in the development of lung damage under simulated microgravity. Further study still clarifies the mechanism about the lung injury under microgravity. VL - 2 IS - 1 ER -
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