Tuberculosis (TB) is an infectious disease caused mainly by bacillus Mycobacterium tuberculosis and it remains a major public health problem. Globally, 9.6 Million people were ill and 1.5 million were died of Tuberculosis in 2014. In resource limited settings, tuberculosis diagnosis relies on sputum smear microscopy: with low and variable sensitivities, in adequately trained personnel, erratic reagent supplies, and poorly verified equipment. Thus there is a critical need for investment in laboratory capacity building and quality assured service. The objective of this study is to assess the performance and trend of Acid Fast Bacilli (AFB) smear microscopy external quality assessment program in Addis Ababa city Government. For this study a retrospective record review study was carried out among the participated AFB smear microscopy laboratories in Addis Ababa. The data was collected and summarized from 12 Jan. 2014 to March 20, 2015 in Addis Ababa Health research laboratory. The data entered in to EPI data V.3 and analyzed by SPSS version 20. Percentage of agreement for smear positive and negative results between periphery diagnostic health facilities and the regional laboratory was 95% and 99%, respectively. They have an observed agreement (Po) of 0.9918 and an expected agreement (Pe) of 0.8290. Moreover, Calculated kappa value was 0.95 which is almost perfect agreement. The trend of discordant slides decreases as participation in Regional External Quality Assurance System (REQAS) increases. This study concludes with evidences from the result that, Quality assured laboratory is the cornerstone for effective tuberculosis control programme. Continuous participation in REQAS and drilling of laboratory personnel accordingly at regular intervals plays an important role for improving the quality of TB laboratory services.
| Published in | American Journal of Laboratory Medicine (Volume 1, Issue 2) |
| DOI | 10.11648/j.ajlm.20160102.12 |
| Page(s) | 9-15 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2016. Published by Science Publishing Group |
EQA, Blinded Rechecking, AFB, Laboratory
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APA Style
Abay Sisay Misganaw, Mulugeta Tsegaye Abebe, Adino Desale Lulie, Abrham Tesfaye Bika. (2016). External Quality Assessment (EQA) of Randomly Blinded Rechecking Slides on TB AFB Microscopy Laboratories: A Retrospective Study, Addis Ababa, Ethiopia. American Journal of Laboratory Medicine, 1(2), 9-15. https://doi.org/10.11648/j.ajlm.20160102.12
ACS Style
Abay Sisay Misganaw; Mulugeta Tsegaye Abebe; Adino Desale Lulie; Abrham Tesfaye Bika. External Quality Assessment (EQA) of Randomly Blinded Rechecking Slides on TB AFB Microscopy Laboratories: A Retrospective Study, Addis Ababa, Ethiopia. Am. J. Lab. Med. 2016, 1(2), 9-15. doi: 10.11648/j.ajlm.20160102.12
AMA Style
Abay Sisay Misganaw, Mulugeta Tsegaye Abebe, Adino Desale Lulie, Abrham Tesfaye Bika. External Quality Assessment (EQA) of Randomly Blinded Rechecking Slides on TB AFB Microscopy Laboratories: A Retrospective Study, Addis Ababa, Ethiopia. Am J Lab Med. 2016;1(2):9-15. doi: 10.11648/j.ajlm.20160102.12
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Download@article{10.11648/j.ajlm.20160102.12,
author = {Abay Sisay Misganaw and Mulugeta Tsegaye Abebe and Adino Desale Lulie and Abrham Tesfaye Bika},
title = {External Quality Assessment (EQA) of Randomly Blinded Rechecking Slides on TB AFB Microscopy Laboratories: A Retrospective Study, Addis Ababa, Ethiopia},
journal = {American Journal of Laboratory Medicine},
volume = {1},
number = {2},
pages = {9-15},
doi = {10.11648/j.ajlm.20160102.12},
url = {https://doi.org/10.11648/j.ajlm.20160102.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20160102.12},
abstract = {Tuberculosis (TB) is an infectious disease caused mainly by bacillus Mycobacterium tuberculosis and it remains a major public health problem. Globally, 9.6 Million people were ill and 1.5 million were died of Tuberculosis in 2014. In resource limited settings, tuberculosis diagnosis relies on sputum smear microscopy: with low and variable sensitivities, in adequately trained personnel, erratic reagent supplies, and poorly verified equipment. Thus there is a critical need for investment in laboratory capacity building and quality assured service. The objective of this study is to assess the performance and trend of Acid Fast Bacilli (AFB) smear microscopy external quality assessment program in Addis Ababa city Government. For this study a retrospective record review study was carried out among the participated AFB smear microscopy laboratories in Addis Ababa. The data was collected and summarized from 12 Jan. 2014 to March 20, 2015 in Addis Ababa Health research laboratory. The data entered in to EPI data V.3 and analyzed by SPSS version 20. Percentage of agreement for smear positive and negative results between periphery diagnostic health facilities and the regional laboratory was 95% and 99%, respectively. They have an observed agreement (Po) of 0.9918 and an expected agreement (Pe) of 0.8290. Moreover, Calculated kappa value was 0.95 which is almost perfect agreement. The trend of discordant slides decreases as participation in Regional External Quality Assurance System (REQAS) increases. This study concludes with evidences from the result that, Quality assured laboratory is the cornerstone for effective tuberculosis control programme. Continuous participation in REQAS and drilling of laboratory personnel accordingly at regular intervals plays an important role for improving the quality of TB laboratory services.},
year = {2016}
}
TY - JOUR T1 - External Quality Assessment (EQA) of Randomly Blinded Rechecking Slides on TB AFB Microscopy Laboratories: A Retrospective Study, Addis Ababa, Ethiopia AU - Abay Sisay Misganaw AU - Mulugeta Tsegaye Abebe AU - Adino Desale Lulie AU - Abrham Tesfaye Bika Y1 - 2016/09/12 PY - 2016 N1 - https://doi.org/10.11648/j.ajlm.20160102.12 DO - 10.11648/j.ajlm.20160102.12 T2 - American Journal of Laboratory Medicine JF - American Journal of Laboratory Medicine JO - American Journal of Laboratory Medicine SP - 9 EP - 15 PB - Science Publishing Group SN - 2575-386X UR - https://doi.org/10.11648/j.ajlm.20160102.12 AB - Tuberculosis (TB) is an infectious disease caused mainly by bacillus Mycobacterium tuberculosis and it remains a major public health problem. Globally, 9.6 Million people were ill and 1.5 million were died of Tuberculosis in 2014. In resource limited settings, tuberculosis diagnosis relies on sputum smear microscopy: with low and variable sensitivities, in adequately trained personnel, erratic reagent supplies, and poorly verified equipment. Thus there is a critical need for investment in laboratory capacity building and quality assured service. The objective of this study is to assess the performance and trend of Acid Fast Bacilli (AFB) smear microscopy external quality assessment program in Addis Ababa city Government. For this study a retrospective record review study was carried out among the participated AFB smear microscopy laboratories in Addis Ababa. The data was collected and summarized from 12 Jan. 2014 to March 20, 2015 in Addis Ababa Health research laboratory. The data entered in to EPI data V.3 and analyzed by SPSS version 20. Percentage of agreement for smear positive and negative results between periphery diagnostic health facilities and the regional laboratory was 95% and 99%, respectively. They have an observed agreement (Po) of 0.9918 and an expected agreement (Pe) of 0.8290. Moreover, Calculated kappa value was 0.95 which is almost perfect agreement. The trend of discordant slides decreases as participation in Regional External Quality Assurance System (REQAS) increases. This study concludes with evidences from the result that, Quality assured laboratory is the cornerstone for effective tuberculosis control programme. Continuous participation in REQAS and drilling of laboratory personnel accordingly at regular intervals plays an important role for improving the quality of TB laboratory services. VL - 1 IS - 2 ER -
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