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The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats

Received: 8 March 2020     Accepted: 3 April 2020     Published: 30 April 2020
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Abstract

The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.

Published in American Journal of Internal Medicine (Volume 8, Issue 3)
DOI 10.11648/j.ajim.20200803.12
Page(s) 101-106
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Diabetes Mellitus, Type 1, Insulin, Periodontitis, Interleukin-18, Leptin, Biologic Markers

References
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    Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, et al. (2020). The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. American Journal of Internal Medicine, 8(3), 101-106. https://doi.org/10.11648/j.ajim.20200803.12

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    ACS Style

    Eudoxie Pepelassi; Ioanna Xynogala; Despina Perrea; Alkistis Pantopoulou; George Agrogiannis, et al. The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. Am. J. Intern. Med. 2020, 8(3), 101-106. doi: 10.11648/j.ajim.20200803.12

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    AMA Style

    Eudoxie Pepelassi, Ioanna Xynogala, Despina Perrea, Alkistis Pantopoulou, George Agrogiannis, et al. The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats. Am J Intern Med. 2020;8(3):101-106. doi: 10.11648/j.ajim.20200803.12

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  • @article{10.11648/j.ajim.20200803.12,
      author = {Eudoxie Pepelassi and Ioanna Xynogala and Despina Perrea and Alkistis Pantopoulou and George Agrogiannis and Ioannis Vrotsos},
      title = {The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats},
      journal = {American Journal of Internal Medicine},
      volume = {8},
      number = {3},
      pages = {101-106},
      doi = {10.11648/j.ajim.20200803.12},
      url = {https://doi.org/10.11648/j.ajim.20200803.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20200803.12},
      abstract = {The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.},
     year = {2020}
    }
    

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  • TY  - JOUR
    T1  - The Effect of Experimental Periodontitis on the Serum Levels of Leptin and IL-18 in Insulin-Treated Diabetic Rats
    AU  - Eudoxie Pepelassi
    AU  - Ioanna Xynogala
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    T2  - American Journal of Internal Medicine
    JF  - American Journal of Internal Medicine
    JO  - American Journal of Internal Medicine
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    EP  - 106
    PB  - Science Publishing Group
    SN  - 2330-4324
    UR  - https://doi.org/10.11648/j.ajim.20200803.12
    AB  - The aim of this study in rats was to assess the effect of ligature-induced periodontitis on insulin-treated type 1 diabetes in terms of serum levels of leptin and interleukin-18 (IL-18). Twenty five male Wistar rats were studied, out of 40 initially used: (1) 14 rats with insulin-treated diabetes (control, DI) and (2) 11 rats with combination of insulin-treated diabetes and periodontitis (test, DIP). For all rats, type 1 diabetes was streptozotocin-induced and insulin treatment started on day 5. For DIP group, periodontitis was induced by ligation on day 16. Serum levels of leptin and IL-18 were assessed on days 16 (baseline) and 77 (final) by using multiplex immunoassay. All rats were sacrificed on day 77 (end of the study). Insulin treatment in the newly-induced diabetes significantly increased serum leptin levels and significantly reduced serum IL-18 levels. In the newly-induced diabetes, the combination of the continuation of insulin treatment and periodontitis non-significantly increased serum leptin and IL-18 levels. Serum IL-18 levels were significantly higher for the combination of periodontitis and insulin-treated diabetes as compared to insulin-treated diabetes alone. Within its limits, this study in rats showed that experimental periodontitis induced changes in serum biomarker levels suggestive of alterations in the systemic inflammation generated by insulin-treated type 1 diabetes.
    VL  - 8
    IS  - 3
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Author Information
  • Department of Periodontology, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece

  • Department of Periodontology, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece

  • Laboratory for Experimental Surgery & Surgical Research “N. S. Christeas”, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

  • Laboratory for Experimental Surgery & Surgical Research “N. S. Christeas”, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

  • 1st Department of Pathology, School of Medicine, National and Kapodistrian University of Athens, Athens, Greece

  • Department of Periodontology, School of Dentistry, National and Kapodistrian University of Athens, Athens, Greece

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