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Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia

Received: 16 June 2013     Published: 10 July 2013
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Abstract

Inflammatory process is not limited only local effects but could affect other organs and tissues. In this study, we investigated the influence of experimental tibial osteomyelitis on skin cells in dynamics. Although the skin located at the distance from the infection nidus it supposed to manifest intensive proliferation, reparation and apoptosis under adverse factors. Immunohistochemical assay of apoptotic regulators and proliferation-related proteins was carried out in fibroblasts of stratum reticulare in Wistar rats. Proliferating fibroblasts pool decrease confirmed by Ki67 expression decrease was revealed, overexpression of p53 and Bax was accompanied by Bcl-2 expression decrease. Findings suggest depressed regeneratory potential of fibroblasts, high probability of apoptosis in the skin cells at the persisting osteomyelitis that should be considered in dermatological practice.

Published in American Journal of Internal Medicine (Volume 1, Issue 2)
DOI 10.11648/j.ajim.20130102.11
Page(s) 10-13
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Osteomyelitis, Tibia, Р53, Bax, Bcl-2, Ki-67, Fibroblasts, Stratum Reticulare, Apoptosis, Staphylococcus Аureus

References
[1] R. S. Akhtar, Y. Geng, B. J. Klocke, K. A. Roth, "Neural precursor cells possess multiple p53-dependent apoptotic pathways ", Cell Death Differ., 2006, vol. 13, pp. 1727-1739.
[2] S. A. Arkhipov, V. A. Shkurupy, M. V. Solomatina, E. S. Akhramenko, D. A. Iljine, "Study of macrophages in BCG granulomas in different compartments of the mononuclear phagocyte system", Bull. Exp. Biol. Med., February 2013, vol. 154 (4), pp. 467-470.
[3] F. Bibeau, F. Boissière-Michot, J. C. Sabourin, "Assessment of epidermal growth factor receptor (EGFR) expression in primary colorectal carcinomas and their related metastases on tissue sections and tissue microarray", Virchows Arch., 2006, vol. 449 (3), pp. 281-287.
[4] О. V. Bukharin, "Significance of bacterial pathogens persistence for clinical practice", Russ. Med. News, 2000, vol. 5 (3), pp. 18-25.
[5] C. Franke, M. Nöldner, R. Abdel-Kader, L. N. Johnson-Anuna, W. Gibson Wood, W. E. Müller, G. P. Eckert, "Bcl-2 upregulation and neuroprotection in guinea pig brain following chronic simvastatin treatment", Neurobiol. Dis., 2007, vol. 25 (2), pp. 438-445.
[6] B. N. Green, C. D. Johnson, J. T. Egan, M. Rosenthal, E.A. Griffith, M. W. Evans, "Methicillin-resistant Staphylococcus aureus: an overview for manual therapists", J. Chiropr. Med., 2012, vol. 11 (1), pp. 64-76.
[7] M. Jarvis, U. Schulz, A. M. Dickinson, L .Sviland, G. Jackson, A. Konur, X. N. Wang, I. Hromadnikova, H. J. Kolb, G. Eissner, E. Holler, "The detection of apoptosis in a human in vitro skin explant assay for graft versus host reactions", J. Clin. Pathol., Feb 2002, vol. 55, pp. 127-132.
[8] S. L. Harris, A. J. Levine, "The p53 pathway: positive and negative feedback loops", Oncogene, 2005, vol. 24. pp. 2899-2908.
[9] V. A. Karpin, "General pathology: chronic infectious process", Succes. Nat. Sс., 2005, vol. 4, pp. 17- 20.
[10] P. J. Lindsberg, A. J. Grau "Inflammation and infections as risk factors for ichemic stroke", Stroke, 2003, Oct, vol. 34, pp. 2518-2532.
[11] Mathieu, L. M. Cruz-Orive, H. Hoppeler, E. R. Weibel "Measuring error and sampling variation in stereology: Comparison of the efficiency of various methods of planar image analysis", J. Microsc., 1981, vol. 121 (1). pp. 75-88.
[12] T. J. Pallasch, M. J. Wahl, "The focal infection theory: appraisal and reappraisal", J. Calif. Dent. Ass., 2000, vol. 28 (3), pp. 194-200.
[13] N. A. Pecareva, L. A. Trunova, T. V. Belousova, O. M. Gorbenko, A. P. Shvayuk, A. N. Trunov, "About activity of immune-inflammation in children with chronic pyelonephritis in clinical remission", Bull. Russ. Acad. Med Sc., 2008, vol. 131 (3), pp. 52-55.
[14] J. A. Ramos-Vara, "Technical Aspects of Immunohistochemistry", Vet. Pathol., 2005, vol. 42 (4), pp. 405-426.
[15] M. Schuler, D. R. Green, "Transcription, apoptosis and p53: Catch- 22", Trends Genet., 2005, vol. 21, pp. 182-187.
[16] J. Yu, L. Zhang, "The transcriptional targets of p53 in apoptosis control", Biochem. Biophys. Res. Commun., 2005, vol. 331, pp. 851-858.
[17] P. Zhurakovsky, S. A. Arkhipov, M. G. Pustovetova , T. A. Kunts, M. V. Bitkhaeva, I. O. Marinkin, "Activation of mitochondrial way hepatocyte apoptosis when the persistence of bacterial infection", Zabaik. Med Bull., 2011, vol. 2, pp. 125-131.
[18] P. Zhurakovsky, S. A. Arkhipov, M. G. Pustovetova , T. A. Kunts, M. V. Bitkhaeva, I. O. Marinkin, "Bcl-2 expression changes in rat liver and cytokines levels in blood serum at the persistence of a bacterial infection", Kuban. Med. Sc. Bull., 2012, vol. 2, pp. 84-88.
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  • APA Style

    Igor Zhurakovsky, Sergey Arkhypov, Marya Pustovetova, Igor Marinkin, Tatyana Kunts. (2013). Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia. American Journal of Internal Medicine, 1(2), 10-13. https://doi.org/10.11648/j.ajim.20130102.11

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    ACS Style

    Igor Zhurakovsky; Sergey Arkhypov; Marya Pustovetova; Igor Marinkin; Tatyana Kunts. Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia. Am. J. Intern. Med. 2013, 1(2), 10-13. doi: 10.11648/j.ajim.20130102.11

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    AMA Style

    Igor Zhurakovsky, Sergey Arkhypov, Marya Pustovetova, Igor Marinkin, Tatyana Kunts. Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia. Am J Intern Med. 2013;1(2):10-13. doi: 10.11648/j.ajim.20130102.11

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  • @article{10.11648/j.ajim.20130102.11,
      author = {Igor Zhurakovsky and Sergey Arkhypov and Marya Pustovetova and Igor Marinkin and Tatyana Kunts},
      title = {Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia},
      journal = {American Journal of Internal Medicine},
      volume = {1},
      number = {2},
      pages = {10-13},
      doi = {10.11648/j.ajim.20130102.11},
      url = {https://doi.org/10.11648/j.ajim.20130102.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajim.20130102.11},
      abstract = {Inflammatory process is not limited only local effects but could affect other organs and tissues. In this study, we investigated the influence of experimental tibial osteomyelitis on skin cells in dynamics.  Although the skin located at the distance from the infection nidus it supposed to manifest intensive proliferation, reparation and apoptosis under adverse factors. Immunohistochemical assay of apoptotic regulators and proliferation-related proteins was carried out in fibroblasts of stratum reticulare in Wistar rats. Proliferating fibroblasts pool decrease confirmed by Ki67 expression decrease was revealed, overexpression of p53 and Bax was accompanied by Bcl-2 expression decrease. Findings suggest depressed regeneratory potential of fibroblasts, high probability of apoptosis in the skin cells at the persisting osteomyelitis that should be considered in dermatological practice.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Functional Status of Skin Fibroblasts at Chronic Staphylococcus Aureus Osteomyelitis of the Tibia
    AU  - Igor Zhurakovsky
    AU  - Sergey Arkhypov
    AU  - Marya Pustovetova
    AU  - Igor Marinkin
    AU  - Tatyana Kunts
    Y1  - 2013/07/10
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ajim.20130102.11
    DO  - 10.11648/j.ajim.20130102.11
    T2  - American Journal of Internal Medicine
    JF  - American Journal of Internal Medicine
    JO  - American Journal of Internal Medicine
    SP  - 10
    EP  - 13
    PB  - Science Publishing Group
    SN  - 2330-4324
    UR  - https://doi.org/10.11648/j.ajim.20130102.11
    AB  - Inflammatory process is not limited only local effects but could affect other organs and tissues. In this study, we investigated the influence of experimental tibial osteomyelitis on skin cells in dynamics.  Although the skin located at the distance from the infection nidus it supposed to manifest intensive proliferation, reparation and apoptosis under adverse factors. Immunohistochemical assay of apoptotic regulators and proliferation-related proteins was carried out in fibroblasts of stratum reticulare in Wistar rats. Proliferating fibroblasts pool decrease confirmed by Ki67 expression decrease was revealed, overexpression of p53 and Bax was accompanied by Bcl-2 expression decrease. Findings suggest depressed regeneratory potential of fibroblasts, high probability of apoptosis in the skin cells at the persisting osteomyelitis that should be considered in dermatological practice.
    VL  - 1
    IS  - 2
    ER  - 

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Author Information
  • Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia

  • Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia

  • Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia

  • Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia

  • Central Research Laboratory, Novosibirsk State Medical University, Novosibirsk, Russia

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