The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis

Rongqin Dai; Yang Chen; Ying Li; Haitao Hu; Jinbiao Zhang

doi:doi:10.11648/j.ajcem.20210901.12

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The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis

Rongqin Dai, Yang Chen, Ying Li, Haitao Hu, Jinbiao Zhang

Published in American Journal of Clinical and Experimental Medicine (Volume 9, Issue 1)

Received: 12 December 2020 Published: 22 January 2021

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Abstract

Background: Hashimoto's Thyroiditis is a common autoimmune thyroid disease of organ specificity mediated by T cells, the abnormal activation of auto-reactive T cells has close relation with the attack of HT and participates in the attack process of HT. TPOAb and TGAb is important symbols of HT thyroiditis. Most of patients have continuous TPOAb and TGAb level rise. Objective: To assess the relationship between T Lymphocyte Subsets and TPOAb & TGAb Level in Patients with Hashimoto's Thyroiditis. Method: 130 clinically diagnosed HT patients are selected as the study object and were divided into high level group and low level group (with 65 cases for each group) as per the expression condition of TPOAb and TGAb; another 40 healthy check-up people are selected as the control group. Flow cytometry is adopted to test the T lymphocyte subsets, and ELISA is adopted to test Th1 cytokine (IL-2, IFN-γ) and Th2cytokine (IL-4, IL-10). Result: CD3⁺T, CD3⁺CD4⁺T and CD4⁺/CD8⁺ of high level TPOAb group and high level TGAb group are obviously higher than that of low level group and the control group, CD3⁺CD8⁺T is obviously lower than that of low level group and the control group; the CD3⁺CD4⁺T of the low level TPOAb group and low level TGAb group is obviously higher than the control group, which has statistic meaning (P<0.05). IL-2 and IFN-γ of the high level TPOAb group and high level TGAb group is obviously lower than the low level group while higher than the control group, and the IL-4 and IL-10 is obviously higher than the low level group and the control group; the IL-2 and IFN-γ of the low level TPOAb group and low level TGAb is obviously higher than that of high level group and the control group, and the IL-4 and IL-10 is obviously lower than that of the high level group and the control group, and the differences all have statistic meaning (P<0.05). Correlation analysis indicates that TPOAb and TGAb are in positive correlation with CD3⁺CD4⁺T and CD4⁺/CD8⁺, while in negative correlation with CD3⁺CD8⁺T. Conclusion: T lymphocyte subsets in the HT patients have obvious correlation with the level of TPOAb and TGAb, and may have close relationship with the occurrence and development of HT.

Published in	American Journal of Clinical and Experimental Medicine (Volume 9, Issue 1)
DOI	10.11648/j.ajcem.20210901.12
Page(s)	8-12
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Hashimoto's Thyroiditis, TPOAb, TGAb, T Lymphocyte Subsets, Cytokines

References

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APA Style

Rongqin Dai, Yang Chen, Ying Li, Haitao Hu, Jinbiao Zhang. (2021). The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis. American Journal of Clinical and Experimental Medicine, 9(1), 8-12. https://doi.org/10.11648/j.ajcem.20210901.12

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ACS Style

Rongqin Dai; Yang Chen; Ying Li; Haitao Hu; Jinbiao Zhang. The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis. Am. J. Clin. Exp. Med. 2021, 9(1), 8-12. doi: 10.11648/j.ajcem.20210901.12

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AMA Style

Rongqin Dai, Yang Chen, Ying Li, Haitao Hu, Jinbiao Zhang. The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis. Am J Clin Exp Med. 2021;9(1):8-12. doi: 10.11648/j.ajcem.20210901.12

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@article{10.11648/j.ajcem.20210901.12,
  author = {Rongqin Dai and Yang Chen and Ying Li and Haitao Hu and Jinbiao Zhang},
  title = {The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {9},
  number = {1},
  pages = {8-12},
  doi = {10.11648/j.ajcem.20210901.12},
  url = {https://doi.org/10.11648/j.ajcem.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20210901.12},
  abstract = {Background: Hashimoto's Thyroiditis is a common autoimmune thyroid disease of organ specificity mediated by T cells, the abnormal activation of auto-reactive T cells has close relation with the attack of HT and participates in the attack process of HT. TPOAb and TGAb is important symbols of HT thyroiditis. Most of patients have continuous TPOAb and TGAb level rise. Objective: To assess the relationship between T Lymphocyte Subsets and TPOAb & TGAb Level in Patients with Hashimoto's Thyroiditis. Method: 130 clinically diagnosed HT patients are selected as the study object and were divided into high level group and low level group (with 65 cases for each group) as per the expression condition of TPOAb and TGAb; another 40 healthy check-up people are selected as the control group. Flow cytometry is adopted to test the T lymphocyte subsets, and ELISA is adopted to test Th1 cytokine (IL-2, IFN-γ) and Th2cytokine (IL-4, IL-10). Result: CD3+T, CD3+CD4+T and CD4+/CD8+ of high level TPOAb group and high level TGAb group are obviously higher than that of low level group and the control group, CD3+CD8+T is obviously lower than that of low level group and the control group; the CD3+CD4+T of the low level TPOAb group and low level TGAb group is obviously higher than the control group, which has statistic meaning (P+CD4+T and CD4+/CD8+, while in negative correlation with CD3+CD8+T. Conclusion: T lymphocyte subsets in the HT patients have obvious correlation with the level of TPOAb and TGAb, and may have close relationship with the occurrence and development of HT.},
 year = {2021}
}

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TY  - JOUR
T1  - The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis
AU  - Rongqin Dai
AU  - Yang Chen
AU  - Ying Li
AU  - Haitao Hu
AU  - Jinbiao Zhang
Y1  - 2021/01/22
PY  - 2021
N1  - https://doi.org/10.11648/j.ajcem.20210901.12
DO  - 10.11648/j.ajcem.20210901.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 8
EP  - 12
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20210901.12
AB  - Background: Hashimoto's Thyroiditis is a common autoimmune thyroid disease of organ specificity mediated by T cells, the abnormal activation of auto-reactive T cells has close relation with the attack of HT and participates in the attack process of HT. TPOAb and TGAb is important symbols of HT thyroiditis. Most of patients have continuous TPOAb and TGAb level rise. Objective: To assess the relationship between T Lymphocyte Subsets and TPOAb & TGAb Level in Patients with Hashimoto's Thyroiditis. Method: 130 clinically diagnosed HT patients are selected as the study object and were divided into high level group and low level group (with 65 cases for each group) as per the expression condition of TPOAb and TGAb; another 40 healthy check-up people are selected as the control group. Flow cytometry is adopted to test the T lymphocyte subsets, and ELISA is adopted to test Th1 cytokine (IL-2, IFN-γ) and Th2cytokine (IL-4, IL-10). Result: CD3+T, CD3+CD4+T and CD4+/CD8+ of high level TPOAb group and high level TGAb group are obviously higher than that of low level group and the control group, CD3+CD8+T is obviously lower than that of low level group and the control group; the CD3+CD4+T of the low level TPOAb group and low level TGAb group is obviously higher than the control group, which has statistic meaning (P+CD4+T and CD4+/CD8+, while in negative correlation with CD3+CD8+T. Conclusion: T lymphocyte subsets in the HT patients have obvious correlation with the level of TPOAb and TGAb, and may have close relationship with the occurrence and development of HT.
VL  - 9
IS  - 1
ER  -

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Author Information

Rongqin Dai

Department of Medical Technology Laboratory, Cangzhou Medical College, Cangzhou, China
Yang Chen

Department of Medical Technology Laboratory, Cangzhou Medical College, Cangzhou, China
Ying Li

Department of Medical Technology Laboratory, Cangzhou Medical College, Cangzhou, China
Haitao Hu

Laboratory Diagnostics Division, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou, China
Jinbiao Zhang

Laboratory Diagnostics Division, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine, Cangzhou, China

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APA Style

Rongqin Dai, Yang Chen, Ying Li, Haitao Hu, Jinbiao Zhang. (2021). The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis. American Journal of Clinical and Experimental Medicine, 9(1), 8-12. https://doi.org/10.11648/j.ajcem.20210901.12

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ACS Style

Rongqin Dai; Yang Chen; Ying Li; Haitao Hu; Jinbiao Zhang. The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis. Am. J. Clin. Exp. Med. 2021, 9(1), 8-12. doi: 10.11648/j.ajcem.20210901.12

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AMA Style

Rongqin Dai, Yang Chen, Ying Li, Haitao Hu, Jinbiao Zhang. The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis. Am J Clin Exp Med. 2021;9(1):8-12. doi: 10.11648/j.ajcem.20210901.12

Copy | Download

@article{10.11648/j.ajcem.20210901.12,
  author = {Rongqin Dai and Yang Chen and Ying Li and Haitao Hu and Jinbiao Zhang},
  title = {The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {9},
  number = {1},
  pages = {8-12},
  doi = {10.11648/j.ajcem.20210901.12},
  url = {https://doi.org/10.11648/j.ajcem.20210901.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20210901.12},
  abstract = {Background: Hashimoto's Thyroiditis is a common autoimmune thyroid disease of organ specificity mediated by T cells, the abnormal activation of auto-reactive T cells has close relation with the attack of HT and participates in the attack process of HT. TPOAb and TGAb is important symbols of HT thyroiditis. Most of patients have continuous TPOAb and TGAb level rise. Objective: To assess the relationship between T Lymphocyte Subsets and TPOAb & TGAb Level in Patients with Hashimoto's Thyroiditis. Method: 130 clinically diagnosed HT patients are selected as the study object and were divided into high level group and low level group (with 65 cases for each group) as per the expression condition of TPOAb and TGAb; another 40 healthy check-up people are selected as the control group. Flow cytometry is adopted to test the T lymphocyte subsets, and ELISA is adopted to test Th1 cytokine (IL-2, IFN-γ) and Th2cytokine (IL-4, IL-10). Result: CD3+T, CD3+CD4+T and CD4+/CD8+ of high level TPOAb group and high level TGAb group are obviously higher than that of low level group and the control group, CD3+CD8+T is obviously lower than that of low level group and the control group; the CD3+CD4+T of the low level TPOAb group and low level TGAb group is obviously higher than the control group, which has statistic meaning (P+CD4+T and CD4+/CD8+, while in negative correlation with CD3+CD8+T. Conclusion: T lymphocyte subsets in the HT patients have obvious correlation with the level of TPOAb and TGAb, and may have close relationship with the occurrence and development of HT.},
 year = {2021}
}

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TY  - JOUR
T1  - The Relationship Between T Lymphocyte Subsets and TPOAb & TGAb Levelin Patients with Hashimoto's Thyroiditis
AU  - Rongqin Dai
AU  - Yang Chen
AU  - Ying Li
AU  - Haitao Hu
AU  - Jinbiao Zhang
Y1  - 2021/01/22
PY  - 2021
N1  - https://doi.org/10.11648/j.ajcem.20210901.12
DO  - 10.11648/j.ajcem.20210901.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 8
EP  - 12
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20210901.12
AB  - Background: Hashimoto's Thyroiditis is a common autoimmune thyroid disease of organ specificity mediated by T cells, the abnormal activation of auto-reactive T cells has close relation with the attack of HT and participates in the attack process of HT. TPOAb and TGAb is important symbols of HT thyroiditis. Most of patients have continuous TPOAb and TGAb level rise. Objective: To assess the relationship between T Lymphocyte Subsets and TPOAb & TGAb Level in Patients with Hashimoto's Thyroiditis. Method: 130 clinically diagnosed HT patients are selected as the study object and were divided into high level group and low level group (with 65 cases for each group) as per the expression condition of TPOAb and TGAb; another 40 healthy check-up people are selected as the control group. Flow cytometry is adopted to test the T lymphocyte subsets, and ELISA is adopted to test Th1 cytokine (IL-2, IFN-γ) and Th2cytokine (IL-4, IL-10). Result: CD3+T, CD3+CD4+T and CD4+/CD8+ of high level TPOAb group and high level TGAb group are obviously higher than that of low level group and the control group, CD3+CD8+T is obviously lower than that of low level group and the control group; the CD3+CD4+T of the low level TPOAb group and low level TGAb group is obviously higher than the control group, which has statistic meaning (P+CD4+T and CD4+/CD8+, while in negative correlation with CD3+CD8+T. Conclusion: T lymphocyte subsets in the HT patients have obvious correlation with the level of TPOAb and TGAb, and may have close relationship with the occurrence and development of HT.
VL  - 9
IS  - 1
ER  -

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