Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors

Sekongo Yassongui Mamadou; Dasse Sery Romuald; Altemeyer Anaïs; Soraya Amar; Tayou Claude; Anani Ludovic; Kassogue Kadidia; Geisen Cristof; Herbrich Anne; Kouamenan Sidonie; Konate Seidou

doi:doi:10.11648/j.ajcem.20190706.11

Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors

Sekongo Yassongui Mamadou, Dasse Sery Romuald, Altemeyer Anaïs, Soraya Amar, Tayou Claude, Anani Ludovic, Kassogue Kadidia, Geisen Cristof, Herbrich Anne, Kouamenan Sidonie, Konate Seidou

Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 6)

Received: 16 October 2019 Accepted: 6 November 2019 Published: 27 November 2019

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Abstract

To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan population in order to guide the design of the clinical study that will use the INTERCEPT Red Blood System procedure on whole blood for transfusion. to define the starting point in the evaluation of the immunological safety and transfusion safety of the product treated by this process. The objective of this study is to determine the prevalence of AAA among blood donors in sub-Saharan Africa. We conducted a multicenter prospective descriptive study of 902 blood donors collected in Côte d'Ivoire, Benin and Cameroon over the period from June 2015 to January 2017. Blood samples were collected from voluntary blood donors, of any sex, aged between 18 and 65, having given their consent for the study and having participated in the medical consultation for the donation of blood. The samples were analyzed according to the technique of the RAI gel card of the company BIORAD after centrifugation and incubation using test red cells treated with S-303 and glutathione. In the case of positive RAI results, to confirm the presence of anti-acridine, the donor plasma should be incubated with S-300. S-300 is a degradation product of S-303. The donor serum and S-300 are then incubated with the same red test cells. S-300 binds to the antibody and produces a negative result in the gel map in the presence of anti-acridin antibodies. Of the 903 samples tested both at the Abidjan laboratory in Côte D'Ivoire and at the Frankfurt laboratory, we found 1 positive sample and 8 reactive samples (positive for anti-erythrocyte antibodies). Positive donor plasma was incubated with S-300 which is the degradation product of S-303. The result is always positive, whereas according to the instructions of the reference laboratory of Frankfurt, it should be negative in case of presence of Locustacan. The results on AAA testing among 903 donors in three sub-Saharan countries show the absence of AAA in the sample of subjects included in the study according to the hypothesis emitted from this study. This opens the door to the prospect of conducting a clinical study on the inactivation of pathogens by acridine derivatives.

Published in	American Journal of Clinical and Experimental Medicine (Volume 7, Issue 6)
DOI	10.11648/j.ajcem.20190706.11
Page(s)	126-129
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Acridine, Immunization, Subsaharian, Blood Donors

References

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[2]	Mirasol Clinical Evaluation Study Group (2010) A randomized controlled clinical trial evaluating the performance and safety of platelets treated with MIRASOL pathogen reduction technology. Transfusion 2010; 50: 2362-2375.
[3]	Johnson L, Winter KM, Reid S, et al. The effect of pathogen reduction technology (Mirasol) on platelet quality when treated in additive solution with low plasma carryover. Vox Sang 2011; 101: Acridine–Wikipedia. Disponible sur https://fr.wikipedia.org/wiki/Acridine.
[4]	Ramesh Kumar, Mandeep Kaur And Meena Kumari Acridine: A Versatile Heterocyclic Nucleus Acta Poloniae Pharmaceutica Drug Research, 2012; 69 (1): 3-9.
[5]	Mufti NA, Erickson AC, North AK, et al. Treatment of whole blood and red blood cells with S-303 inactivates pathogens and retains in vitro quality of stored RBC. Biologicals 2010; 38: 14-9.
[6]	Henschler R, Seifried E, Mufti N. Development of the S-303 pathogen inactivation technology for red blood cell concentrates. Transfus Med Hemother 2011; 38: 33-42.
[7]	So-Yong Kwon, I S Kim, J. E. Bae and Al. Pathogen inactivation efficacy of Mirasol PRT System and Intercept Blood System for non-leucoreduced platelet-rich plasma-derived platelets suspended in plasma. Vox Sanguinis, 2014; 107 (3).
[8]	Winter KM, Johnson L, Kwok M, Vidovic D, Hyland RA, Mufti N, et al. Red blood cell in vitro quality and function is maintained after S-303 pathogen inactivation treatment. Transfusion 2014; 54 (7): 1798-807.
[9]	Erickson A, Donnelly B, Schott M, et al. In vitro evaluation of pathogen inactivated RBC using the S-303 treatment system. Transfusion 2012; 52: 77A.
[10]	Erickson A, Schott MA, Sherman C, et al. In vitro evaluation of pathogen inactivated RBC using the S-303 treatment system [abstract]. Vox Sang 2011; 101: 78.
[11]	North A, Ciaravino V, Mufti N, et al. Preclinical pharmacokinetic and toxicology assessment of red blood cells prepared with S-303 pathogen inactivation treatment. Transfusion 2011; 51: 2208-18.
[12]	Organic syntheses. In Synthesis of acridone 19: 6; Coll. Vol. 2: 15 orgsyn. org [archives} from o-chlorobenzoic acid and aniline in a Goldberg reaction.
[13]	Organic syntheses. Synthesis of 9-aminoacridine 22: 5; Coll. Vol. 3: 53. orgsyn. org [archive}] from N-phenylanthranilic acid.
[14]	Gerard P. Moloney, David P. Kelly, P. Mack Synthesis of Acridine-based DNA Bis-intercalating Agents Molecules 2001; 6: 230-243.
[15]	C. Geisen, V. Brixner, L. Stempniewski, A. North, A.-H. Kiessling, M. M. Müller, N. Mufti, E. Seifried. Characterization of preexisting antibodies to S-303 pathogen inactivated red blood cells (S-303 RBC) in patient sera. International Society of Blood Transfusion (ISBT) congress, Septembre 2014, Seoul.

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Sekongo Yassongui Mamadou, Dasse Sery Romuald, Altemeyer Anaïs, Soraya Amar, Tayou Claude, et al. (2019). Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors. American Journal of Clinical and Experimental Medicine, 7(6), 126-129. https://doi.org/10.11648/j.ajcem.20190706.11

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ACS Style

Sekongo Yassongui Mamadou; Dasse Sery Romuald; Altemeyer Anaïs; Soraya Amar; Tayou Claude, et al. Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors. Am. J. Clin. Exp. Med. 2019, 7(6), 126-129. doi: 10.11648/j.ajcem.20190706.11

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AMA Style

Sekongo Yassongui Mamadou, Dasse Sery Romuald, Altemeyer Anaïs, Soraya Amar, Tayou Claude, et al. Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors. Am J Clin Exp Med. 2019;7(6):126-129. doi: 10.11648/j.ajcem.20190706.11

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@article{10.11648/j.ajcem.20190706.11,
  author = {Sekongo Yassongui Mamadou and Dasse Sery Romuald and Altemeyer Anaïs and Soraya Amar and Tayou Claude and Anani Ludovic and Kassogue Kadidia and Geisen Cristof and Herbrich Anne and Kouamenan Sidonie and Konate Seidou},
  title = {Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {6},
  pages = {126-129},
  doi = {10.11648/j.ajcem.20190706.11},
  url = {https://doi.org/10.11648/j.ajcem.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190706.11},
  abstract = {To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan population in order to guide the design of the clinical study that will use the INTERCEPT Red Blood System procedure on whole blood for transfusion. to define the starting point in the evaluation of the immunological safety and transfusion safety of the product treated by this process. The objective of this study is to determine the prevalence of AAA among blood donors in sub-Saharan Africa. We conducted a multicenter prospective descriptive study of 902 blood donors collected in Côte d'Ivoire, Benin and Cameroon over the period from June 2015 to January 2017. Blood samples were collected from voluntary blood donors, of any sex, aged between 18 and 65, having given their consent for the study and having participated in the medical consultation for the donation of blood. The samples were analyzed according to the technique of the RAI gel card of the company BIORAD after centrifugation and incubation using test red cells treated with S-303 and glutathione. In the case of positive RAI results, to confirm the presence of anti-acridine, the donor plasma should be incubated with S-300. S-300 is a degradation product of S-303. The donor serum and S-300 are then incubated with the same red test cells. S-300 binds to the antibody and produces a negative result in the gel map in the presence of anti-acridin antibodies. Of the 903 samples tested both at the Abidjan laboratory in Côte D'Ivoire and at the Frankfurt laboratory, we found 1 positive sample and 8 reactive samples (positive for anti-erythrocyte antibodies). Positive donor plasma was incubated with S-300 which is the degradation product of S-303. The result is always positive, whereas according to the instructions of the reference laboratory of Frankfurt, it should be negative in case of presence of Locustacan. The results on AAA testing among 903 donors in three sub-Saharan countries show the absence of AAA in the sample of subjects included in the study according to the hypothesis emitted from this study. This opens the door to the prospect of conducting a clinical study on the inactivation of pathogens by acridine derivatives.},
 year = {2019}
}

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TY  - JOUR
T1  - Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors
AU  - Sekongo Yassongui Mamadou
AU  - Dasse Sery Romuald
AU  - Altemeyer Anaïs
AU  - Soraya Amar
AU  - Tayou Claude
AU  - Anani Ludovic
AU  - Kassogue Kadidia
AU  - Geisen Cristof
AU  - Herbrich Anne
AU  - Kouamenan Sidonie
AU  - Konate Seidou
Y1  - 2019/11/27
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190706.11
DO  - 10.11648/j.ajcem.20190706.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 126
EP  - 129
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190706.11
AB  - To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan population in order to guide the design of the clinical study that will use the INTERCEPT Red Blood System procedure on whole blood for transfusion. to define the starting point in the evaluation of the immunological safety and transfusion safety of the product treated by this process. The objective of this study is to determine the prevalence of AAA among blood donors in sub-Saharan Africa. We conducted a multicenter prospective descriptive study of 902 blood donors collected in Côte d'Ivoire, Benin and Cameroon over the period from June 2015 to January 2017. Blood samples were collected from voluntary blood donors, of any sex, aged between 18 and 65, having given their consent for the study and having participated in the medical consultation for the donation of blood. The samples were analyzed according to the technique of the RAI gel card of the company BIORAD after centrifugation and incubation using test red cells treated with S-303 and glutathione. In the case of positive RAI results, to confirm the presence of anti-acridine, the donor plasma should be incubated with S-300. S-300 is a degradation product of S-303. The donor serum and S-300 are then incubated with the same red test cells. S-300 binds to the antibody and produces a negative result in the gel map in the presence of anti-acridin antibodies. Of the 903 samples tested both at the Abidjan laboratory in Côte D'Ivoire and at the Frankfurt laboratory, we found 1 positive sample and 8 reactive samples (positive for anti-erythrocyte antibodies). Positive donor plasma was incubated with S-300 which is the degradation product of S-303. The result is always positive, whereas according to the instructions of the reference laboratory of Frankfurt, it should be negative in case of presence of Locustacan. The results on AAA testing among 903 donors in three sub-Saharan countries show the absence of AAA in the sample of subjects included in the study according to the hypothesis emitted from this study. This opens the door to the prospect of conducting a clinical study on the inactivation of pathogens by acridine derivatives.
VL  - 7
IS  - 6
ER  -

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Author Information

Sekongo Yassongui Mamadou

Department of Training and Research, National Blood Transfusion Center, Abidjan
Dasse Sery Romuald

Hospitality and University Center of Cocody, Abidjan, C?te D’Ivoire
Altemeyer Anaïs
Soraya Amar

Transfusion Swiss Red Cross, Bernn, Switzerland
Tayou Claude

Haematology and Blood Transfusion Service of University Teaching Hospital, Yaoundé, Cameroon
Anani Ludovic

Blood Transfusion Agency, Cotonou, Benin
Kassogue Kadidia

Department of Training and Research, National Blood Transfusion Center, Abidjan
Geisen Cristof

Laboratory, Institute of Transfusion Medicine and Immunohematology, Frankfurt, German
Herbrich Anne

Laboratory, Institute of Transfusion Medicine and Immunohematology, Frankfurt, German
Kouamenan Sidonie

Department of Training and Research, National Blood Transfusion Center, Abidjan
Konate Seidou

The National Blood Transfusion Center, Abidjan, C?te d’Ivoire

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APA Style

Sekongo Yassongui Mamadou, Dasse Sery Romuald, Altemeyer Anaïs, Soraya Amar, Tayou Claude, et al. (2019). Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors. American Journal of Clinical and Experimental Medicine, 7(6), 126-129. https://doi.org/10.11648/j.ajcem.20190706.11

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ACS Style

Sekongo Yassongui Mamadou; Dasse Sery Romuald; Altemeyer Anaïs; Soraya Amar; Tayou Claude, et al. Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors. Am. J. Clin. Exp. Med. 2019, 7(6), 126-129. doi: 10.11648/j.ajcem.20190706.11

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AMA Style

Sekongo Yassongui Mamadou, Dasse Sery Romuald, Altemeyer Anaïs, Soraya Amar, Tayou Claude, et al. Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors. Am J Clin Exp Med. 2019;7(6):126-129. doi: 10.11648/j.ajcem.20190706.11

Copy | Download

@article{10.11648/j.ajcem.20190706.11,
  author = {Sekongo Yassongui Mamadou and Dasse Sery Romuald and Altemeyer Anaïs and Soraya Amar and Tayou Claude and Anani Ludovic and Kassogue Kadidia and Geisen Cristof and Herbrich Anne and Kouamenan Sidonie and Konate Seidou},
  title = {Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {6},
  pages = {126-129},
  doi = {10.11648/j.ajcem.20190706.11},
  url = {https://doi.org/10.11648/j.ajcem.20190706.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190706.11},
  abstract = {To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan population in order to guide the design of the clinical study that will use the INTERCEPT Red Blood System procedure on whole blood for transfusion. to define the starting point in the evaluation of the immunological safety and transfusion safety of the product treated by this process. The objective of this study is to determine the prevalence of AAA among blood donors in sub-Saharan Africa. We conducted a multicenter prospective descriptive study of 902 blood donors collected in Côte d'Ivoire, Benin and Cameroon over the period from June 2015 to January 2017. Blood samples were collected from voluntary blood donors, of any sex, aged between 18 and 65, having given their consent for the study and having participated in the medical consultation for the donation of blood. The samples were analyzed according to the technique of the RAI gel card of the company BIORAD after centrifugation and incubation using test red cells treated with S-303 and glutathione. In the case of positive RAI results, to confirm the presence of anti-acridine, the donor plasma should be incubated with S-300. S-300 is a degradation product of S-303. The donor serum and S-300 are then incubated with the same red test cells. S-300 binds to the antibody and produces a negative result in the gel map in the presence of anti-acridin antibodies. Of the 903 samples tested both at the Abidjan laboratory in Côte D'Ivoire and at the Frankfurt laboratory, we found 1 positive sample and 8 reactive samples (positive for anti-erythrocyte antibodies). Positive donor plasma was incubated with S-300 which is the degradation product of S-303. The result is always positive, whereas according to the instructions of the reference laboratory of Frankfurt, it should be negative in case of presence of Locustacan. The results on AAA testing among 903 donors in three sub-Saharan countries show the absence of AAA in the sample of subjects included in the study according to the hypothesis emitted from this study. This opens the door to the prospect of conducting a clinical study on the inactivation of pathogens by acridine derivatives.},
 year = {2019}
}

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TY  - JOUR
T1  - Prevalence of Acridine Immunization to Subsaharians Africans Blood Donors
AU  - Sekongo Yassongui Mamadou
AU  - Dasse Sery Romuald
AU  - Altemeyer Anaïs
AU  - Soraya Amar
AU  - Tayou Claude
AU  - Anani Ludovic
AU  - Kassogue Kadidia
AU  - Geisen Cristof
AU  - Herbrich Anne
AU  - Kouamenan Sidonie
AU  - Konate Seidou
Y1  - 2019/11/27
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190706.11
DO  - 10.11648/j.ajcem.20190706.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 126
EP  - 129
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190706.11
AB  - To conduct a study project on the inactivation of pathogens in whole blood using acridine derivatives in Africa, in order to prevent the transmission of pathogens, it is important to look for possible immunization to acridine in the blood donor population. It is therefore important to undertake an estimate of the prevalence in the sub-Saharan population in order to guide the design of the clinical study that will use the INTERCEPT Red Blood System procedure on whole blood for transfusion. to define the starting point in the evaluation of the immunological safety and transfusion safety of the product treated by this process. The objective of this study is to determine the prevalence of AAA among blood donors in sub-Saharan Africa. We conducted a multicenter prospective descriptive study of 902 blood donors collected in Côte d'Ivoire, Benin and Cameroon over the period from June 2015 to January 2017. Blood samples were collected from voluntary blood donors, of any sex, aged between 18 and 65, having given their consent for the study and having participated in the medical consultation for the donation of blood. The samples were analyzed according to the technique of the RAI gel card of the company BIORAD after centrifugation and incubation using test red cells treated with S-303 and glutathione. In the case of positive RAI results, to confirm the presence of anti-acridine, the donor plasma should be incubated with S-300. S-300 is a degradation product of S-303. The donor serum and S-300 are then incubated with the same red test cells. S-300 binds to the antibody and produces a negative result in the gel map in the presence of anti-acridin antibodies. Of the 903 samples tested both at the Abidjan laboratory in Côte D'Ivoire and at the Frankfurt laboratory, we found 1 positive sample and 8 reactive samples (positive for anti-erythrocyte antibodies). Positive donor plasma was incubated with S-300 which is the degradation product of S-303. The result is always positive, whereas according to the instructions of the reference laboratory of Frankfurt, it should be negative in case of presence of Locustacan. The results on AAA testing among 903 donors in three sub-Saharan countries show the absence of AAA in the sample of subjects included in the study according to the hypothesis emitted from this study. This opens the door to the prospect of conducting a clinical study on the inactivation of pathogens by acridine derivatives.
VL  - 7
IS  - 6
ER  -

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