Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes

Tingting Chen; Donghui Tian; Yurong Zhang

doi:doi:10.11648/j.ajcem.20190702.13

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Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes

Tingting Chen, Donghui Tian, Yurong Zhang

Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2)

Received: 29 May 2019 Published: 29 July 2019

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Abstract

Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.

Published in	American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2)
DOI	10.11648/j.ajcem.20190702.13
Page(s)	54-60
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Epstein-Barr Virus, Gastric Cancer, Methylation, Tumor Suppressor Gene

References

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APA Style

Tingting Chen, Donghui Tian, Yurong Zhang. (2019). Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. American Journal of Clinical and Experimental Medicine, 7(2), 54-60. https://doi.org/10.11648/j.ajcem.20190702.13

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ACS Style

Tingting Chen; Donghui Tian; Yurong Zhang. Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. Am. J. Clin. Exp. Med. 2019, 7(2), 54-60. doi: 10.11648/j.ajcem.20190702.13

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AMA Style

Tingting Chen, Donghui Tian, Yurong Zhang. Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. Am J Clin Exp Med. 2019;7(2):54-60. doi: 10.11648/j.ajcem.20190702.13

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@article{10.11648/j.ajcem.20190702.13,
  author = {Tingting Chen and Donghui Tian and Yurong Zhang},
  title = {Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {2},
  pages = {54-60},
  doi = {10.11648/j.ajcem.20190702.13},
  url = {https://doi.org/10.11648/j.ajcem.20190702.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190702.13},
  abstract = {Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.},
 year = {2019}
}

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TY  - JOUR
T1  - Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes
AU  - Tingting Chen
AU  - Donghui Tian
AU  - Yurong Zhang
Y1  - 2019/07/29
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190702.13
DO  - 10.11648/j.ajcem.20190702.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 54
EP  - 60
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190702.13
AB  - Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.
VL  - 7
IS  - 2
ER  -

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Author Information

Tingting Chen

Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China
Donghui Tian

Department of Neurology, Binzhou People's Hospital, Binzhou City, P. R. China
Yurong Zhang

Department of Hepatobiliary Surgery, Binzhou People's Hospital, Binzhou City, P. R. China

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APA Style

Tingting Chen, Donghui Tian, Yurong Zhang. (2019). Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. American Journal of Clinical and Experimental Medicine, 7(2), 54-60. https://doi.org/10.11648/j.ajcem.20190702.13

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ACS Style

Tingting Chen; Donghui Tian; Yurong Zhang. Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. Am. J. Clin. Exp. Med. 2019, 7(2), 54-60. doi: 10.11648/j.ajcem.20190702.13

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AMA Style

Tingting Chen, Donghui Tian, Yurong Zhang. Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes. Am J Clin Exp Med. 2019;7(2):54-60. doi: 10.11648/j.ajcem.20190702.13

Copy | Download

@article{10.11648/j.ajcem.20190702.13,
  author = {Tingting Chen and Donghui Tian and Yurong Zhang},
  title = {Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {2},
  pages = {54-60},
  doi = {10.11648/j.ajcem.20190702.13},
  url = {https://doi.org/10.11648/j.ajcem.20190702.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190702.13},
  abstract = {Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.},
 year = {2019}
}

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TY  - JOUR
T1  - Methylation of Epstein-Barr Virus-Associated Gastric Cancer Suppressor Genes
AU  - Tingting Chen
AU  - Donghui Tian
AU  - Yurong Zhang
Y1  - 2019/07/29
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190702.13
DO  - 10.11648/j.ajcem.20190702.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 54
EP  - 60
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190702.13
AB  - Objective: To identify the methylation silenced tumor suppressor genes in Epstein-Barr virus (EBV)-associated gastric cancer (EBVaGC). Methods: EBV-positive (GT38, PT and SNU719) and negative (SGC7901) gastric cancer cell lines were selected and treated with 5-Aza-CdR. Then real-time fluorescence quantitative PCR was used to validate the results of microarray, and methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were adopted to detect the CpG island methylation levels of gene promoters. Results: The expression levels of 6 differentially expressed genes (H19, LOXL1, ARMCX2, LXN, CDH3 and MMP7) before and after 5-Aza-CdR treatment were confirmed by real-time qPCR, which are consistent with the results of microarray analysis. There were different degrees of methylation in LOXL1 gene promoter in EBVaGC. GT38 and PT were fully methylated, and SGC7901 and HGC-27 was unmethylated, suggesting that this gene is a candidate methylation silenced tumor suppressor gene. The methylation rate of LOXL1 in EBVaGC was significantly higher than that in EBV-negative gastric cancer (EBVnGC). Conclusion: The promoter region of candidate tumor suppressor gene LOXL1 shows high methylation status, indicating that EBV critically accounts for the methylation of LOXL1 gene regulatory region. EBV is involved in the pathogensis of EBVaGC that aberrant methylation occurred in promoter CpG island, which inactivates tumor suppressor genes.
VL  - 7
IS  - 2
ER  -

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