Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats

Yonghua Liu; Zhijuan Zhou; Zhiling Zhu; Wenyi Tang; Liyun Luo; Chen Jian

doi:doi:10.11648/j.ajcem.20190702.12

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Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats

Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, Chen Jian

Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2)

Received: 8 April 2019 Accepted: 9 May 2019 Published: 9 July 2019

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Abstract

[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.

Published in	American Journal of Clinical and Experimental Medicine (Volume 7, Issue 2)
DOI	10.11648/j.ajcem.20190702.12
Page(s)	47-53
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Acute Myocardial Infarction, Angiogenesis, L-Arginine

References

[1]	Singh P, Sethi N, Kaur N, et al. Revascularization in Severe Left Ventricular Dysfunction: Does Myocardial Viability Even Matter [J]? Clin Med Insights Cardiol, 2015, 9 (Suppl 1): 105-9.
[2]	Lassaletta, A. D. Chu, L. M. Sellke, F. W. Therapeutic neovascularization for coronary disease: current state and future prospects [J]. Basic Res Cardiol, 2011, 106 (6): 897-909.
[3]	Takeda Y, Uemura S, Iwama H, et al. Treatment with recombinant placental growth factor (PlGF) enhances both angiogenesis and arteriogenesis and improves survival after myocardial infarction [J]. Circ J, 2009, 73 (9): 1674-82.
[4]	Voisine P, Bianchi C, Khan TA, et al. Normalization of coronary microvascular reactivity and improvement in myocardial perfusion by surgical vascular endothelial growth factor combined with oral supplementation of L-Arginine in a porcine model of endothelial dysfunction. J Thoral cardiocasc Surg, 2005, 129 (6): 1414-20.
[5]	He Jin, Xie Xiumei, Fangye Qing, et al. L-Arginine inhibits the proliferation of endothelial progenitor cells induced by asymmetric dimethylarginine. Chinese Journal of Arteriosclerosis, 2007, 15 (10): 751-754.
[6]	J Chen, S Zheng, H Huang, et al. Mesenchymal Stem Cells Enhanced Cardiac Nerve Sprouting via Nerve Growth Factor in a Rat Model of Myocardial Infarction [J]. Current Pharmaceutical Design, 2014, 20 (12): 2023-2029.
[7]	Wykrzykowska JJ, Rosinberg A, Lee SU, et al. Autologous cardiomyotissue implantation promotes myocardial regeneration, decreases infarct size, and improves left ventricular function [J]. Circulation, 2011, 123 (1): 62-9.
[8]	Chen G, Nayan M, Duong M, et al. Marrow stromal cells for cell-based therapy: the role of antiinflammatory cytokines in cellular cardiomyoplasty [J]. Ann Thorac Surg, 2010, 90 (1): 190-7.
[9]	Li J, Yin FF, Hou YL. Early diagnosis of rats with acute myocardial infarction by measurement of brain natriuretic peptide [J]. Exp Ther Med, 2013, 5 (4): 1201- 1205.
[10]	Rendell MS, Finnegan MF, Pisarri T, et al. et al. A comparison of the cutaneous microvascular properties of the spontaneously hypertensive rat and the Wistar-Kyoto rat [J]. Comp Biochem Physiol A Mol Integr Physiol, 1999, 122 (4): 399-406.
[11]	Voisine P, Bianchi C, Khan TA, et al. Normalization of coronary microvascular reactivity and improvement in myocardial perfusion by surgical vascular endothelial growth factor combined with oral supplementation of L-arginine in a porcine model of endothelial dysfunction [J]. J Thoral cardiocasc Surg, 2005, 129 (6): 1414-20.
[12]	Society of Cardiology, Chinese Medical Association. Guidelines for diagnosis and treatment of acute ST-segment elevation myocardial infarction in 2015. Chinese Journal of Cardiovascular Disease, 2015, 5: 380-393.
[13]	Lu Dongfeng, Longguanzhou, Shi Shumei. Decreased expression of endothelial nitric oxide synthase gene and increased expression of endothelial nitric oxide synthase gene by L-Arginine in rats with acute myocardial infarction. Chinese Journal of Cardiovascular Disease, 2004, 10 (32): 713.
[14]	Gray GA, Patrizio M, Sherry L, et al. Immunolocalisation and activity of DDAH I and II in the heart and modification post-myocardial infarction. Acta Histochem, 2010, 112 (5): 413-23.
[15]	Theilmeier G, Chan JR, Zalpour S, et al. Adhesiveness of Mononuclear Cells in Hyperchole sterolemic Humans is normalized by Dietary L-Arginine. Arteriosclero Thromb Vasc Biol, 1997, 17: 3557–3564.
[16]	Martínez-Romero R, Cañuelo A, Siles E, et al. Nitric oxide modulates hypoxia-inducible factor-1 and poly (ADP-ribose) polymerase-1 cross talk in response to hypobaric hypoxia. J Appl Physiol, 2012, 112 (5): 816-23.
[17]	Fraccarollo D, Widder JD, Galuppo P, et al. Improvement in left ventricular remodeling by the endothelial nitric oxide synthase enhancer AVE9488 after experimental myocardial infarction. Circulation, 2008, 118: 818–827.
[18]	Landmesser U, Engberding N, Bahlmann FH, et al. Statin-induced improvement of endothelial progenitor cell mobilization, myocardial neovascularization, left ventricular function, and survival after experimental myocardial infarction requires endothelial nitric oxide synthase. Circulation, 2004, 110: 1933–1939.
[19]	Bornstein P. Matricellular proteins: an overview [J]. J Cell Commun Signal, 2009, 3 (3-4): 163-5.
[20]	Lu, Z, X. Mao, L. L. Lian, F. et al. Cardioprotective activity of placental growth factor in a rat model of acute myocardial infarction: nanoparticle-based delivery versus direct myocardial injection [J]. BMC Cardiovasc Disord, 2014, 17; 14: 53.
[21]	Antonio D, Lassaletta Louis M, Chu Frank W, et al. Therapeutic neovascularization for coronary disease: current state and future prospects [J]. Basic Res Cardiol, 2011, 106 (6): 897-909.

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APA Style

Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, et al. (2019). Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. American Journal of Clinical and Experimental Medicine, 7(2), 47-53. https://doi.org/10.11648/j.ajcem.20190702.12

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ACS Style

Yonghua Liu; Zhijuan Zhou; Zhiling Zhu; Wenyi Tang; Liyun Luo, et al. Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. Am. J. Clin. Exp. Med. 2019, 7(2), 47-53. doi: 10.11648/j.ajcem.20190702.12

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AMA Style

Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, et al. Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. Am J Clin Exp Med. 2019;7(2):47-53. doi: 10.11648/j.ajcem.20190702.12

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@article{10.11648/j.ajcem.20190702.12,
  author = {Yonghua Liu and Zhijuan Zhou and Zhiling Zhu and Wenyi Tang and Liyun Luo and Chen Jian},
  title = {Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {2},
  pages = {47-53},
  doi = {10.11648/j.ajcem.20190702.12},
  url = {https://doi.org/10.11648/j.ajcem.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190702.12},
  abstract = {[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.},
 year = {2019}
}

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TY  - JOUR
T1  - Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats
AU  - Yonghua Liu
AU  - Zhijuan Zhou
AU  - Zhiling Zhu
AU  - Wenyi Tang
AU  - Liyun Luo
AU  - Chen Jian
Y1  - 2019/07/09
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190702.12
DO  - 10.11648/j.ajcem.20190702.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 47
EP  - 53
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190702.12
AB  - [Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.
VL  - 7
IS  - 2
ER  -

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Author Information

Yonghua Liu

Department of Interventional Medical Center, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Zhijuan Zhou

Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Zhiling Zhu

Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Wenyi Tang

Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Liyun Luo

Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China
Chen Jian

Department of Cardiology, the Fifth Affiliated Hospital of Sun Yat-Sen University, Zhuhai, People's Republic of China

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APA Style

Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, et al. (2019). Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. American Journal of Clinical and Experimental Medicine, 7(2), 47-53. https://doi.org/10.11648/j.ajcem.20190702.12

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ACS Style

Yonghua Liu; Zhijuan Zhou; Zhiling Zhu; Wenyi Tang; Liyun Luo, et al. Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. Am. J. Clin. Exp. Med. 2019, 7(2), 47-53. doi: 10.11648/j.ajcem.20190702.12

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AMA Style

Yonghua Liu, Zhijuan Zhou, Zhiling Zhu, Wenyi Tang, Liyun Luo, et al. Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats. Am J Clin Exp Med. 2019;7(2):47-53. doi: 10.11648/j.ajcem.20190702.12

Copy | Download

@article{10.11648/j.ajcem.20190702.12,
  author = {Yonghua Liu and Zhijuan Zhou and Zhiling Zhu and Wenyi Tang and Liyun Luo and Chen Jian},
  title = {Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {2},
  pages = {47-53},
  doi = {10.11648/j.ajcem.20190702.12},
  url = {https://doi.org/10.11648/j.ajcem.20190702.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190702.12},
  abstract = {[Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - Oral Supplementation of L-Arginine Improves Ventricular Remodeling by Regulating Angiogenesis and Collagen Synthesis in Myocardial Infarction Rats
AU  - Yonghua Liu
AU  - Zhijuan Zhou
AU  - Zhiling Zhu
AU  - Wenyi Tang
AU  - Liyun Luo
AU  - Chen Jian
Y1  - 2019/07/09
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190702.12
DO  - 10.11648/j.ajcem.20190702.12
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 47
EP  - 53
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190702.12
AB  - [Objective] To investigate the effect of angiogenesis and collagen synthesis in peri-infarct area of the L-Arginine therapy for acute myocardial infarction rats. [Methods] The acute myocardial infarction rats model was established by ligation of the left anterior descending of coronary artery. Thirty male Sprague-Dawley rats were randomly divided into three groups: L-Arginine group, sham group, normal saline group (NS group). Four weeks after ligation, cardiac function, scar area, plasma concentration of BNP, angiogenesis and arteriogenesis, myocardial collagen I and eNOS protein, the mRNA expression of collagen I were studied. Echocardiography, Masson staining, enzyme-linked immunosorbent assay (ELISA), immunehistochemistry, western blot and quantitative polymerase chain (qPCR) reaction were performed. [Results] Four weeks after ligation, compared with the control group, LVEF, LVFS were higher in L-Arginine group, While LVEDD and LVESD decreased (P < 0.01). Average scar percentage and plasma concentration of BNP were lower in L-Arginine group (P < 0.01). The CD31-positive microvessels and α-SMA positive microvessels in peri-infarct area were higher in L-Arginine group (P < 0.01), while collagen I protein and mRNA expression was decreased in this group (P < 0.01). [Conclusions] L-Arginine improves cardiac function and reduces infarction size in AMI rats, the possible mechanism is related to dual function of promoting angiogenesis and arteriogenesis, regulating collagen I expression is also one of the important mechanisms.
VL  - 7
IS  - 2
ER  -

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