A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene

Tingting Chen; Hongna Lv; Yanping Xu; Wenjing Du; Rui Zhang

doi:doi:10.11648/j.ajcem.20190701.11

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A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene

Tingting Chen, Hongna Lv, Yanping Xu, Wenjing Du, Rui Zhang

Published in American Journal of Clinical and Experimental Medicine (Volume 7, Issue 1)

Received: 17 January 2019 Published: 1 February 2019

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Abstract

The aim of this paper is to study the mechanism of MLH1 gene expression and regulation in colorectal cancer, and to clarify the impact of 5' end CpG island aberrant methylation on the regulation of MLH1 gene expression. RT-PCR assay was used to detect the expression of MLH1 gene mRNA in 10 colon cancer cell strains. 62 fresh colorectal adenocarcinoma samples and paired distal normal mucosa were selected in our hospital. They comprised 37 males and 25 females aging 32 to 67 years old with the mean of 43.6. The cellular RNA was extracted, and immunohistochemistry assay was used to detect the expression of MLH1 protein in colorectal cancer, and MSP method was utilized to examine the methylation status of the first exon of MLH1 gene in the tissues. In the 10 cell strains, only the MLH1 mRNAs of SW480 and Caco2 were expressed, and no MLH1 mRNA expression was detected in the other eight strains. The expression level of MLH1 mRNA in the 62 cases of cancer tissue was higher than that in the paired normal mucosa, and MLH1 protein was mainly expressed in the cytoplasm. The expression level of MLH1 protein in adenocarcinoma tissues was significantly higher than that in the paired normal tissue (P<0.01), while the methylation level of cancer tissues was statistically significantly lower than that of the paired normal mucosa (P<0.05). Aberrant DNA methylation dominates the regulation of the MLH1 gene expression changes in colorectal cancer. The results herein provide a theoretical basis for clarifying the differential expressions of MLH1 in colorectal cancer in vitro and in vivo.

Published in	American Journal of Clinical and Experimental Medicine (Volume 7, Issue 1)
DOI	10.11648/j.ajcem.20190701.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2019. Published by Science Publishing Group

Keywords

Rectal Cancer, Colon Cancer, MLH1, Gene Expression and Regulation, Aberrant Methylation

References

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APA Style

Tingting Chen, Hongna Lv, Yanping Xu, Wenjing Du, Rui Zhang. (2019). A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene. American Journal of Clinical and Experimental Medicine, 7(1), 1-6. https://doi.org/10.11648/j.ajcem.20190701.11

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ACS Style

Tingting Chen; Hongna Lv; Yanping Xu; Wenjing Du; Rui Zhang. A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene. Am. J. Clin. Exp. Med. 2019, 7(1), 1-6. doi: 10.11648/j.ajcem.20190701.11

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AMA Style

Tingting Chen, Hongna Lv, Yanping Xu, Wenjing Du, Rui Zhang. A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene. Am J Clin Exp Med. 2019;7(1):1-6. doi: 10.11648/j.ajcem.20190701.11

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@article{10.11648/j.ajcem.20190701.11,
  author = {Tingting Chen and Hongna Lv and Yanping Xu and Wenjing Du and Rui Zhang},
  title = {A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajcem.20190701.11},
  url = {https://doi.org/10.11648/j.ajcem.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190701.11},
  abstract = {The aim of this paper is to study the mechanism of MLH1 gene expression and regulation in colorectal cancer, and to clarify the impact of 5' end CpG island aberrant methylation on the regulation of MLH1 gene expression. RT-PCR assay was used to detect the expression of MLH1 gene mRNA in 10 colon cancer cell strains. 62 fresh colorectal adenocarcinoma samples and paired distal normal mucosa were selected in our hospital. They comprised 37 males and 25 females aging 32 to 67 years old with the mean of 43.6. The cellular RNA was extracted, and immunohistochemistry assay was used to detect the expression of MLH1 protein in colorectal cancer, and MSP method was utilized to examine the methylation status of the first exon of MLH1 gene in the tissues. In the 10 cell strains, only the MLH1 mRNAs of SW480 and Caco2 were expressed, and no MLH1 mRNA expression was detected in the other eight strains. The expression level of MLH1 mRNA in the 62 cases of cancer tissue was higher than that in the paired normal mucosa, and MLH1 protein was mainly expressed in the cytoplasm. The expression level of MLH1 protein in adenocarcinoma tissues was significantly higher than that in the paired normal tissue (P<0.01), while the methylation level of cancer tissues was statistically significantly lower than that of the paired normal mucosa (P<0.05). Aberrant DNA methylation dominates the regulation of the MLH1 gene expression changes in colorectal cancer. The results herein provide a theoretical basis for clarifying the differential expressions of MLH1 in colorectal cancer in vitro and in vivo.},
 year = {2019}
}

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TY  - JOUR
T1  - A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene
AU  - Tingting Chen
AU  - Hongna Lv
AU  - Yanping Xu
AU  - Wenjing Du
AU  - Rui Zhang
Y1  - 2019/02/01
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190701.11
DO  - 10.11648/j.ajcem.20190701.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190701.11
AB  - The aim of this paper is to study the mechanism of MLH1 gene expression and regulation in colorectal cancer, and to clarify the impact of 5' end CpG island aberrant methylation on the regulation of MLH1 gene expression. RT-PCR assay was used to detect the expression of MLH1 gene mRNA in 10 colon cancer cell strains. 62 fresh colorectal adenocarcinoma samples and paired distal normal mucosa were selected in our hospital. They comprised 37 males and 25 females aging 32 to 67 years old with the mean of 43.6. The cellular RNA was extracted, and immunohistochemistry assay was used to detect the expression of MLH1 protein in colorectal cancer, and MSP method was utilized to examine the methylation status of the first exon of MLH1 gene in the tissues. In the 10 cell strains, only the MLH1 mRNAs of SW480 and Caco2 were expressed, and no MLH1 mRNA expression was detected in the other eight strains. The expression level of MLH1 mRNA in the 62 cases of cancer tissue was higher than that in the paired normal mucosa, and MLH1 protein was mainly expressed in the cytoplasm. The expression level of MLH1 protein in adenocarcinoma tissues was significantly higher than that in the paired normal tissue (P<0.01), while the methylation level of cancer tissues was statistically significantly lower than that of the paired normal mucosa (P<0.05). Aberrant DNA methylation dominates the regulation of the MLH1 gene expression changes in colorectal cancer. The results herein provide a theoretical basis for clarifying the differential expressions of MLH1 in colorectal cancer in vitro and in vivo.
VL  - 7
IS  - 1
ER  -

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Author Information

Tingting Chen

Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China
Hongna Lv

Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China
Yanping Xu

Department of Gastroenterology, Binzhou People's Hospital, Binzhou City, P. R. China
Wenjing Du

Technical Office, Binzhou Intermediate People's Court, Binzhou City, P. R. China
Rui Zhang

Technical Office, People's Court of Bincheng District, Binzhou City, P. R. China

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APA Style

Tingting Chen, Hongna Lv, Yanping Xu, Wenjing Du, Rui Zhang. (2019). A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene. American Journal of Clinical and Experimental Medicine, 7(1), 1-6. https://doi.org/10.11648/j.ajcem.20190701.11

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ACS Style

Tingting Chen; Hongna Lv; Yanping Xu; Wenjing Du; Rui Zhang. A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene. Am. J. Clin. Exp. Med. 2019, 7(1), 1-6. doi: 10.11648/j.ajcem.20190701.11

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AMA Style

Tingting Chen, Hongna Lv, Yanping Xu, Wenjing Du, Rui Zhang. A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene. Am J Clin Exp Med. 2019;7(1):1-6. doi: 10.11648/j.ajcem.20190701.11

Copy | Download

@article{10.11648/j.ajcem.20190701.11,
  author = {Tingting Chen and Hongna Lv and Yanping Xu and Wenjing Du and Rui Zhang},
  title = {A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {7},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ajcem.20190701.11},
  url = {https://doi.org/10.11648/j.ajcem.20190701.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20190701.11},
  abstract = {The aim of this paper is to study the mechanism of MLH1 gene expression and regulation in colorectal cancer, and to clarify the impact of 5' end CpG island aberrant methylation on the regulation of MLH1 gene expression. RT-PCR assay was used to detect the expression of MLH1 gene mRNA in 10 colon cancer cell strains. 62 fresh colorectal adenocarcinoma samples and paired distal normal mucosa were selected in our hospital. They comprised 37 males and 25 females aging 32 to 67 years old with the mean of 43.6. The cellular RNA was extracted, and immunohistochemistry assay was used to detect the expression of MLH1 protein in colorectal cancer, and MSP method was utilized to examine the methylation status of the first exon of MLH1 gene in the tissues. In the 10 cell strains, only the MLH1 mRNAs of SW480 and Caco2 were expressed, and no MLH1 mRNA expression was detected in the other eight strains. The expression level of MLH1 mRNA in the 62 cases of cancer tissue was higher than that in the paired normal mucosa, and MLH1 protein was mainly expressed in the cytoplasm. The expression level of MLH1 protein in adenocarcinoma tissues was significantly higher than that in the paired normal tissue (P<0.01), while the methylation level of cancer tissues was statistically significantly lower than that of the paired normal mucosa (P<0.05). Aberrant DNA methylation dominates the regulation of the MLH1 gene expression changes in colorectal cancer. The results herein provide a theoretical basis for clarifying the differential expressions of MLH1 in colorectal cancer in vitro and in vivo.},
 year = {2019}
}

Copy | Download

TY  - JOUR
T1  - A Study on the Aberrant Methylation of Colorectal Cancer MLH1 Gene
AU  - Tingting Chen
AU  - Hongna Lv
AU  - Yanping Xu
AU  - Wenjing Du
AU  - Rui Zhang
Y1  - 2019/02/01
PY  - 2019
N1  - https://doi.org/10.11648/j.ajcem.20190701.11
DO  - 10.11648/j.ajcem.20190701.11
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20190701.11
AB  - The aim of this paper is to study the mechanism of MLH1 gene expression and regulation in colorectal cancer, and to clarify the impact of 5' end CpG island aberrant methylation on the regulation of MLH1 gene expression. RT-PCR assay was used to detect the expression of MLH1 gene mRNA in 10 colon cancer cell strains. 62 fresh colorectal adenocarcinoma samples and paired distal normal mucosa were selected in our hospital. They comprised 37 males and 25 females aging 32 to 67 years old with the mean of 43.6. The cellular RNA was extracted, and immunohistochemistry assay was used to detect the expression of MLH1 protein in colorectal cancer, and MSP method was utilized to examine the methylation status of the first exon of MLH1 gene in the tissues. In the 10 cell strains, only the MLH1 mRNAs of SW480 and Caco2 were expressed, and no MLH1 mRNA expression was detected in the other eight strains. The expression level of MLH1 mRNA in the 62 cases of cancer tissue was higher than that in the paired normal mucosa, and MLH1 protein was mainly expressed in the cytoplasm. The expression level of MLH1 protein in adenocarcinoma tissues was significantly higher than that in the paired normal tissue (P<0.01), while the methylation level of cancer tissues was statistically significantly lower than that of the paired normal mucosa (P<0.05). Aberrant DNA methylation dominates the regulation of the MLH1 gene expression changes in colorectal cancer. The results herein provide a theoretical basis for clarifying the differential expressions of MLH1 in colorectal cancer in vitro and in vivo.
VL  - 7
IS  - 1
ER  -

Copy | Download