Transcriptome Analysis Reveals Multiple Pathways of <i>Lobelia chinensis</i> in Inhibiting <i>Streptococcus pyogenes</i>

Xiaoying Lin; Xiangyu Kong; Chengping Wen; Zhixing He

doi:doi:10.11648/j.ajcem.20180602.13

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Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes

Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He

Published in American Journal of Clinical and Experimental Medicine (Volume 6, Issue 2)

Received: 27 March 2018 Accepted: 15 April 2018 Published: 19 May 2018

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Abstract

Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.

Published in	American Journal of Clinical and Experimental Medicine (Volume 6, Issue 2)
DOI	10.11648/j.ajcem.20180602.13
Page(s)	46-57
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Lobelia chinensis, Streptococcus pyogenes, Penicillin, Transcriptome

References

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APA Style

Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He. (2018). Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. American Journal of Clinical and Experimental Medicine, 6(2), 46-57. https://doi.org/10.11648/j.ajcem.20180602.13

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ACS Style

Xiaoying Lin; Xiangyu Kong; Chengping Wen; Zhixing He. Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. Am. J. Clin. Exp. Med. 2018, 6(2), 46-57. doi: 10.11648/j.ajcem.20180602.13

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AMA Style

Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He. Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. Am J Clin Exp Med. 2018;6(2):46-57. doi: 10.11648/j.ajcem.20180602.13

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@article{10.11648/j.ajcem.20180602.13,
  author = {Xiaoying Lin and Xiangyu Kong and Chengping Wen and Zhixing He},
  title = {Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {6},
  number = {2},
  pages = {46-57},
  doi = {10.11648/j.ajcem.20180602.13},
  url = {https://doi.org/10.11648/j.ajcem.20180602.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180602.13},
  abstract = {Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.},
 year = {2018}
}

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TY  - JOUR
T1  - Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes
AU  - Xiaoying Lin
AU  - Xiangyu Kong
AU  - Chengping Wen
AU  - Zhixing He
Y1  - 2018/05/19
PY  - 2018
N1  - https://doi.org/10.11648/j.ajcem.20180602.13
DO  - 10.11648/j.ajcem.20180602.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 46
EP  - 57
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20180602.13
AB  - Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.
VL  - 6
IS  - 2
ER  -

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Author Information

Xiaoying Lin

College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China
Xiangyu Kong

College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China
Chengping Wen

College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China
Zhixing He

College of Basic Medical Science, Zhejiang Chinese Medical University, Hangzhou, China

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Plain Text BibTeX RIS

APA Style

Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He. (2018). Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. American Journal of Clinical and Experimental Medicine, 6(2), 46-57. https://doi.org/10.11648/j.ajcem.20180602.13

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ACS Style

Xiaoying Lin; Xiangyu Kong; Chengping Wen; Zhixing He. Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. Am. J. Clin. Exp. Med. 2018, 6(2), 46-57. doi: 10.11648/j.ajcem.20180602.13

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AMA Style

Xiaoying Lin, Xiangyu Kong, Chengping Wen, Zhixing He. Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes. Am J Clin Exp Med. 2018;6(2):46-57. doi: 10.11648/j.ajcem.20180602.13

Copy | Download

@article{10.11648/j.ajcem.20180602.13,
  author = {Xiaoying Lin and Xiangyu Kong and Chengping Wen and Zhixing He},
  title = {Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {6},
  number = {2},
  pages = {46-57},
  doi = {10.11648/j.ajcem.20180602.13},
  url = {https://doi.org/10.11648/j.ajcem.20180602.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20180602.13},
  abstract = {Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.},
 year = {2018}
}

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TY  - JOUR
T1  - Transcriptome Analysis Reveals Multiple Pathways of Lobelia chinensis in Inhibiting Streptococcus pyogenes
AU  - Xiaoying Lin
AU  - Xiangyu Kong
AU  - Chengping Wen
AU  - Zhixing He
Y1  - 2018/05/19
PY  - 2018
N1  - https://doi.org/10.11648/j.ajcem.20180602.13
DO  - 10.11648/j.ajcem.20180602.13
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 46
EP  - 57
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20180602.13
AB  - Clinically, Lobelia chinensis has the potential to treat Streptococcus pyogenes (GAS) infections. This study demonstrated that Lobelia chinensis and penicillin have comparative inhibitory effects when their concentration was 12 mg/mL. To uncover the possible pathways of inhibition of GAS by Lobelia chinensis, transcriptome analysis was used to explore significantly changed genes when GAS was cultured under Lobelia chinensi. Lobelia chinensis could induce alterations of 366 genes in expression level, mainly involving biosynthesis process, translation, cytoplasm, and lipid, carbohydrate metabolic process. In addition, penicillin only induced 17 genes alteration and no GO/KEGG pathway enrichment. Therefore, Lobelia chinensis showed more modes of regulating GAS than penicillin. The regulatory modes of Lobelia chinensis may be the inhibition of cell replication and growth of GAS. This study indicated that Lobelia chinens is a potential drug for the treatment of GAS infection due to its considerable inhibition effects and multiple inhibition modes.
VL  - 6
IS  - 2
ER  -

Copy | Download